- Email: [email protected]
Antiproliferative effects of Evernic acid on A549 and healthy human cells: An in vitro study
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Abstracts / Journal of Biotechnology 208 (2015) S5–S120
model system for screening antivirals among plant extracts using forager honeybees naturally infected with Black Queen Cell Virus (BQCV). BQCV is the most prevalent virus that persists as a covert infection in honeybees. This positive-sense ssRNA virus belongs to the well-known Dicistroviridae family, sharing common features with hepatitis C virus. We developed studies on the antiviral role of European propolis (bee glue, plant resins) and Artemisia absinthium, Laurus nobilis leaf extracts that are rich in bioactive compounds and were proven to have antiviral activities in other systems. Among the tested extracts, the Artemisia extract did not show any antiviral effect and propolis extracts showed only a weak trend in the reduction of the virus. Just the Laurus extract led to a significant decrease of virus replication and overall virus titer in forager honeybees. We conclude that using our newly developed BQCV infection-forager bee assay is a time saving and stress reducing approach for bees that can be applied up to a medium and high-throughput screening model system for antiviral drugs under natural conditions. Moreover, using insects as model system brings valuable information regarding the impact of different xenobiotics on a living organism. http://dx.doi.org/10.1016/j.jbiotec.2015.06.073 Antiproliferative effects of Evernic acid on A549 and healthy human cells: An in vitro study Hamit Emre Kizil 1,∗ , Guleray Agar 3 , Mustafa Anar 2 1 Vocational School of Health Services, Bayburt University, Bayburt, Turkey 2 Department of Molecular Biology and Genetics, Ataturk University, Erzurum, Turkey 3 Department of Biology, Ataturk University, Erzurum, Turkey
E-mail address: [email protected] (H. E. Kizil). In this study, we aimed to determine the anticancer effects of different doses of various lichen’s bioactive secondary metabolite evernic acid in A549 cancer cell line and healthy HUVEC cells. RTCA (RealTime Cell Analyser) – SP (Single Plate) system was used to assess the cell viability of HUVEC cell line to be used as control. Proliferative effects of evernic acid were evaluated by WST-1 (water-soluble tetrazolium salts) test. Due to the RTCA-SP results, after the incubation of HUVEC cells, 12.5 g/mL, 25 g/mL, 50 g/mL, 100 g/mL, 200 g/mL doses of evernic acid were added to wells and it was observed that whole doses of evernic acid did not show significant decrease of cell proliferation on healthy human cells. WST-1 assay was performed on A549 cells and observed that 12.5 g/mL, 25 g/mL, 50 g/mL, and 100 g/mL doses were decreased the cell proliferation. These results indicate that 12.5 g/mL, 25 g/mL, 50 g/mL and 100 g/mL were appropriate doses for treatment. Results revealed that evernic acid inhibited the cell proliferation on cancer cells and 12.5 g/mL, 25 g/mL, 50 g/mL and 100 g/mL concentrations are optimum for treatment. Finally, we can assert evernic acid can be an anticancer agent and it will be a candidate compound for the new advanced cancer treatment methods. http://dx.doi.org/10.1016/j.jbiotec.2015.06.074
On-line prediction of antibody titer by Raman spectroscopy and chemometrics Silvère André 1,∗ , Lydia Saint Cristau 2 , Sabine Gaillard 2 , Olivier Devos 1 , Éric Calvosa 2 , Ludovic Duponchel 1 1 Laboratoire de Spectrochimie Infrarouge et Raman, Université de Lille, Villeneuve d’Ascq, France 2 Sanofi Pasteur, Marcy-l’Étoile, France
E-mail address: [email protected] (S. André). In 2004, the Food and Drug Administration started up the Process Analytical Technology (PAT) to encourage industries to use new technologies in order to control every step of their processes. This initiative leads to develop innovative measurement systems, in particular, spectroscopic techniques coupled with chemometrics, which offer short analysis time and on-line measurements. Raman spectroscopy has been successfully applied in biotechnology, but the monitoring of recombinant antibody concentration stays a real challenge for vaccine manufacturers. The current ways to measure antibody titers prevent any feedback on the culture since the reference methods are off-line. In that way, a method to determine on-line the antibody titer could bring a real improvement in cell culture. The present work is supported by the CellPAT project. We have developed a chemometric model, based on in-situ Raman spectra which predict the recombinant antibody titer in real time throughout the cell culture with comparable accuracy with reference methods. http://dx.doi.org/10.1016/j.jbiotec.2015.06.075 Measuring the cytotoxicity of bioactive N-heterocyclic compounds obtained via enzymatic catalysis Ioana Otilia Ghinea 1,∗ , Andreea Veronica Dediu 2 , Claudia Ungureanu 2 , Bianca Furdui 1 , Gabriela Bahrim 2 , Rodica Mihaela Dinica 1 1 Department of Chemistry, Physics and Environment, “Dunarea de Jos” University, Galati, Romania 2 Department of Food Science, Food Engineering and Applied Biotechnology, “Dunarea de Jos” University, Galati, Romania
E-mail address: [email protected] (I. O. Ghinea). The purpose of this study was to evaluate the cytotoxicity induced by the N-heterocyclic compounds through survival tests on Saccharomyces cerevisiae, a microorganism which has several genetic and biochemical characteristics similar to human cells. The tested compounds were synthesized via enzymatic catalysis and were shown to have antioxidant, antibacterial and anti-acetylcholinesterase activity. In this study, we used a pure culture of Saccharomyces cerevisiae MIUG D 27. Two groups of compounds were evaluated: quaternary pyridinium salts and indolizine derivatives obtained through biocatalytic cyclization reactions. Biological models have long been used to determine the cytotoxicity and the cytostatic activity of many chemical compounds natural and/or synthetic. The multiplication, budding and fermentation process of yeast cells were evaluated during submerged cultivation in culture medium containing different concentrations of chemical agents. The results showed that the test was able to clearly differentiate the
Abstracts / Journal of Biotechnology 208 (2015) S5–S120
model system for screening antivirals among plant extracts using forager honeybees naturally infected with Black Queen Cell Virus (BQCV). BQCV is the most prevalent virus that persists as a covert infection in honeybees. This positive-sense ssRNA virus belongs to the well-known Dicistroviridae family, sharing common features with hepatitis C virus. We developed studies on the antiviral role of European propolis (bee glue, plant resins) and Artemisia absinthium, Laurus nobilis leaf extracts that are rich in bioactive compounds and were proven to have antiviral activities in other systems. Among the tested extracts, the Artemisia extract did not show any antiviral effect and propolis extracts showed only a weak trend in the reduction of the virus. Just the Laurus extract led to a significant decrease of virus replication and overall virus titer in forager honeybees. We conclude that using our newly developed BQCV infection-forager bee assay is a time saving and stress reducing approach for bees that can be applied up to a medium and high-throughput screening model system for antiviral drugs under natural conditions. Moreover, using insects as model system brings valuable information regarding the impact of different xenobiotics on a living organism. http://dx.doi.org/10.1016/j.jbiotec.2015.06.073 Antiproliferative effects of Evernic acid on A549 and healthy human cells: An in vitro study Hamit Emre Kizil 1,∗ , Guleray Agar 3 , Mustafa Anar 2 1 Vocational School of Health Services, Bayburt University, Bayburt, Turkey 2 Department of Molecular Biology and Genetics, Ataturk University, Erzurum, Turkey 3 Department of Biology, Ataturk University, Erzurum, Turkey
E-mail address: [email protected] (H. E. Kizil). In this study, we aimed to determine the anticancer effects of different doses of various lichen’s bioactive secondary metabolite evernic acid in A549 cancer cell line and healthy HUVEC cells. RTCA (RealTime Cell Analyser) – SP (Single Plate) system was used to assess the cell viability of HUVEC cell line to be used as control. Proliferative effects of evernic acid were evaluated by WST-1 (water-soluble tetrazolium salts) test. Due to the RTCA-SP results, after the incubation of HUVEC cells, 12.5 g/mL, 25 g/mL, 50 g/mL, 100 g/mL, 200 g/mL doses of evernic acid were added to wells and it was observed that whole doses of evernic acid did not show significant decrease of cell proliferation on healthy human cells. WST-1 assay was performed on A549 cells and observed that 12.5 g/mL, 25 g/mL, 50 g/mL, and 100 g/mL doses were decreased the cell proliferation. These results indicate that 12.5 g/mL, 25 g/mL, 50 g/mL and 100 g/mL were appropriate doses for treatment. Results revealed that evernic acid inhibited the cell proliferation on cancer cells and 12.5 g/mL, 25 g/mL, 50 g/mL and 100 g/mL concentrations are optimum for treatment. Finally, we can assert evernic acid can be an anticancer agent and it will be a candidate compound for the new advanced cancer treatment methods. http://dx.doi.org/10.1016/j.jbiotec.2015.06.074
On-line prediction of antibody titer by Raman spectroscopy and chemometrics Silvère André 1,∗ , Lydia Saint Cristau 2 , Sabine Gaillard 2 , Olivier Devos 1 , Éric Calvosa 2 , Ludovic Duponchel 1 1 Laboratoire de Spectrochimie Infrarouge et Raman, Université de Lille, Villeneuve d’Ascq, France 2 Sanofi Pasteur, Marcy-l’Étoile, France
E-mail address: [email protected] (S. André). In 2004, the Food and Drug Administration started up the Process Analytical Technology (PAT) to encourage industries to use new technologies in order to control every step of their processes. This initiative leads to develop innovative measurement systems, in particular, spectroscopic techniques coupled with chemometrics, which offer short analysis time and on-line measurements. Raman spectroscopy has been successfully applied in biotechnology, but the monitoring of recombinant antibody concentration stays a real challenge for vaccine manufacturers. The current ways to measure antibody titers prevent any feedback on the culture since the reference methods are off-line. In that way, a method to determine on-line the antibody titer could bring a real improvement in cell culture. The present work is supported by the CellPAT project. We have developed a chemometric model, based on in-situ Raman spectra which predict the recombinant antibody titer in real time throughout the cell culture with comparable accuracy with reference methods. http://dx.doi.org/10.1016/j.jbiotec.2015.06.075 Measuring the cytotoxicity of bioactive N-heterocyclic compounds obtained via enzymatic catalysis Ioana Otilia Ghinea 1,∗ , Andreea Veronica Dediu 2 , Claudia Ungureanu 2 , Bianca Furdui 1 , Gabriela Bahrim 2 , Rodica Mihaela Dinica 1 1 Department of Chemistry, Physics and Environment, “Dunarea de Jos” University, Galati, Romania 2 Department of Food Science, Food Engineering and Applied Biotechnology, “Dunarea de Jos” University, Galati, Romania
E-mail address: [email protected] (I. O. Ghinea). The purpose of this study was to evaluate the cytotoxicity induced by the N-heterocyclic compounds through survival tests on Saccharomyces cerevisiae, a microorganism which has several genetic and biochemical characteristics similar to human cells. The tested compounds were synthesized via enzymatic catalysis and were shown to have antioxidant, antibacterial and anti-acetylcholinesterase activity. In this study, we used a pure culture of Saccharomyces cerevisiae MIUG D 27. Two groups of compounds were evaluated: quaternary pyridinium salts and indolizine derivatives obtained through biocatalytic cyclization reactions. Biological models have long been used to determine the cytotoxicity and the cytostatic activity of many chemical compounds natural and/or synthetic. The multiplication, budding and fermentation process of yeast cells were evaluated during submerged cultivation in culture medium containing different concentrations of chemical agents. The results showed that the test was able to clearly differentiate the