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Biomedical and biological applications of isotachophoresis
Journal of Chroiiatogmphy. 225 (1981) Biomedical Applkations Elsevier Scientific Publishmg Company,
CHROMBIO.
Book
539-540 Amsterdam
-Printed
in The Netherlands
968
Review
.and biological applications of isotachophoresis (Froc. 1st Syrnp., Baconfoy, May 4-F&1979) (Analytical Chemistry Symposia Series, Vol. 5), edited by A- Adam and C. Schots, Elsevier, Amsterdam, Oxford, New York, 1980, VII + 278 pp., price Dfl. 120.00, US$58.50, ISBN 0-444-41891-l.
Biomedical
Biochemistry and the biological sciences including medicine require increasingly demanding and more numerous tasks in the analysis of ionogenic components of biological samples. High accuracy and speed in quantitative determinations are especially required, with a simultaneous high degree of qualitative resolution of trace amounts of various compounds. In this respect isotachophoresis has been used increasingly in recent years in addition to classical electromigration methods. The specific advantage of isotachophoresis of the carrierless type consists in its simple quantitative interpretation together with the possibility of automation of the microanalytical separation process. Isotachophoresis, especially in its capillary form, is used in inorganic and organic analysis, including, microanalysis of ionogenic biomacromolecules. In order to extend the application of isotachophoresis it is necessary to become acquainted not only with the principle of the technique, but also with the possibilities of using various electrolyte systems and the way of selecting them. Therefore the publication of Biochemical and Biological Applications of Isotachophoresis should be welcomed_ This book surveys and collects for the first time data on the present-day use of isotachophoresis in biochemistry, biology, pharmacology and toxicology_ The 278 pages of text comprise 24 review articles of which the majority (21 articles) are devoted to practical applications of capillary isotachophoresis. In the introductory part a survey of indispensable theoretical information is presented in an accessible form; this can be recommended especially to those readers who are meeting the method for the first time and/or intend to make active use of it, The description of the newly conceived equipment and the adaptations of commercial capillary apparatus are presented in two articles. This part is suitable for those who are interested in the processing of samples with a high content of salts. The technique of preparative and analytical isotachophoresis in gel is discussed in two articles.
A large part of the text (10 articles) is devoted to the analysis of the proteins or their interaction with drugs. For doctors the analyses of the celebrospinal fluid will surely be of great interest. Five articles are devoted to the analysis of urine
components; the rest of the text includes kinetic studies of enzymatic processes in -ribosornal liver fractions, the analysis of nucleic acid components, lowmolecular-weight carboxylic acids, etc. The electrolyte systems suitable for the analysis of the above-mentioned groups of substances given in this book are of especial value. The book describes a relatively large number of analytical application possibilities with the accent on practical microanalytical use of the available commercial instruments for capilkuy isotachophoresis. It is a pity that the organizers of the symposium have not provided the book with a subject index to ease the reader’s rapid orientation in the problems of this relatively young methodological approach_ The book can provide a good source of information both for scientists and for those working in biochemistry, biology, pharma cy and medicine who intend to introduce isotachophoresis into their laboratories as a routine microanalytical method_ Prague (Czechoslovakia)
Z_ PRUSfK
CHROMBIO.
Book
539-540 Amsterdam
-Printed
in The Netherlands
968
Review
.and biological applications of isotachophoresis (Froc. 1st Syrnp., Baconfoy, May 4-F&1979) (Analytical Chemistry Symposia Series, Vol. 5), edited by A- Adam and C. Schots, Elsevier, Amsterdam, Oxford, New York, 1980, VII + 278 pp., price Dfl. 120.00, US$58.50, ISBN 0-444-41891-l.
Biomedical
Biochemistry and the biological sciences including medicine require increasingly demanding and more numerous tasks in the analysis of ionogenic components of biological samples. High accuracy and speed in quantitative determinations are especially required, with a simultaneous high degree of qualitative resolution of trace amounts of various compounds. In this respect isotachophoresis has been used increasingly in recent years in addition to classical electromigration methods. The specific advantage of isotachophoresis of the carrierless type consists in its simple quantitative interpretation together with the possibility of automation of the microanalytical separation process. Isotachophoresis, especially in its capillary form, is used in inorganic and organic analysis, including, microanalysis of ionogenic biomacromolecules. In order to extend the application of isotachophoresis it is necessary to become acquainted not only with the principle of the technique, but also with the possibilities of using various electrolyte systems and the way of selecting them. Therefore the publication of Biochemical and Biological Applications of Isotachophoresis should be welcomed_ This book surveys and collects for the first time data on the present-day use of isotachophoresis in biochemistry, biology, pharmacology and toxicology_ The 278 pages of text comprise 24 review articles of which the majority (21 articles) are devoted to practical applications of capillary isotachophoresis. In the introductory part a survey of indispensable theoretical information is presented in an accessible form; this can be recommended especially to those readers who are meeting the method for the first time and/or intend to make active use of it, The description of the newly conceived equipment and the adaptations of commercial capillary apparatus are presented in two articles. This part is suitable for those who are interested in the processing of samples with a high content of salts. The technique of preparative and analytical isotachophoresis in gel is discussed in two articles.
A large part of the text (10 articles) is devoted to the analysis of the proteins or their interaction with drugs. For doctors the analyses of the celebrospinal fluid will surely be of great interest. Five articles are devoted to the analysis of urine
components; the rest of the text includes kinetic studies of enzymatic processes in -ribosornal liver fractions, the analysis of nucleic acid components, lowmolecular-weight carboxylic acids, etc. The electrolyte systems suitable for the analysis of the above-mentioned groups of substances given in this book are of especial value. The book describes a relatively large number of analytical application possibilities with the accent on practical microanalytical use of the available commercial instruments for capilkuy isotachophoresis. It is a pity that the organizers of the symposium have not provided the book with a subject index to ease the reader’s rapid orientation in the problems of this relatively young methodological approach_ The book can provide a good source of information both for scientists and for those working in biochemistry, biology, pharma cy and medicine who intend to introduce isotachophoresis into their laboratories as a routine microanalytical method_ Prague (Czechoslovakia)
Z_ PRUSfK