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Bronchial basement membrane thickening and increased laminin deposition in children with chronic obstructive respiratory diseases
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Posters / Paediatric Respiratory Reviews 14S2 (2013) S55–S85
the paucity of evidence-based data. Consensus guidelines for the preoperative management of asthmatic children are needed. A03-17 Alveolar nitric oxide contribution to assess asthma control 1
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O. Sardon , P. Corcuera , A. Aldasoro , J. Korta , J. Mintegui , J.I. Emparanza3 , E.G. Perez ´ Yarza1 . 1 Department of Pediatrics, Division of Respiratory Medicine, University Hospital Donostia; Medical School (U.D. San Sebastian), University of the Basque Country, Department of Pediatrics, San Sebastian, Spain; 2 Department of Pediatrics, Division of Respiratory Medicine, University Hospital Donostia, San Sebastian, Spain; 3 University Donostia Hospital Epidemiology Unit (CIBER-ESP), San Sebastian, Spain Objective: Alveolar nitric oxide measurement to study the association among clinical symptoms, lung function and nitric oxide (NO) in asthmatic children. Materials and Methods: A cross-sectional study with prospective data collection was performed in healthy and asthmatic children aged between 6–16 years old. Exhaled NO at multiple flows (50, 100 and 200 ml/s) was carried out by CLD88sp® analyzer. According to the two-compartment model, alveolar NO concentration (CANO ) and maximum airway NO flux (J awNO ) were calculated. In asthmatic patients, a questionnaire for asthma control (CAN) was completed and a forced spirometry was performed. Quantitative variables were FENO,50 , CANO , J awNO , CAN, FEV1 , FVC, FEV1 /FVC and FEF25–75 . The association among FENO,50 , CANO , J awNO , FEV1 and CAN was studied using Spearman’s rho and the degree of agreement for asthma control was studied using Cohen’s kappa. Results: We studied 162 children; 49 were healthy (group 1), 23 were asthmatic without treatment (group 2), 62 were asthmatic treated with inhaled corticosteroid (ICS) (group 3) and 18 were asthmatic treated with long-acting b2 -agonist and ICS (group 4). CANO (ppb) was 2.3 (0.1–6.7); 3 (0.2–9.2); 2.45 (0.1–24) and 2.1 (0.5–7.3) respectively. J awNO (pl/s) was 540 (98.33–1983); 2356.67 (120–6110); 1445.83 (156–11805) and 1282.5 (250–5636) respectively. The degree of agreement between FENO,50 and J awNO was substantial in all groups. No agreement was found to establish the degree of asthma control among the other variables. Conclusions: The results for CANO and J awNO in healthy children were similar to the findings of other studies published. There was no agreement among the three measurement instruments analyzed to assess the degree of asthma control. A04-180 Analysis of allergen in children with asthma in Changchun city H. Cheng, L. Liu, Y.-N. Li, H.-J. Cheng. First Hospital of Jilin University, Changchun, China Objective: To explore the distribution and character of allergens in children with asthma in Changchun. Method: 610 cases of asthma patients (3–14years old) who admitted to the pediatric pulmonary department of Jilin University in 2006– 2011 were detected the concentration of specific IgE by Allergy Screen method. Results: 290 in 610 got positive results in the test of 14 allergens, the total positive rate was 47.54%. As to the common allergens the positive rate in mites was the highest: 21.31%. Close after that was cat fur, dog fur and grass: 20%. Mold, beef and lamb, and milk also took a good part of it: 13%, 11% and 8%. Among the inhale groups, preschool-age children and school-age children had little difference (P > 0.05). Among the food allergen groups, infants in the rate of allergen would be higher (P > 0.01). The positive rate of allergen of preschool-age children had no difference between inhalational and food allergens groups, while in school-age children, the positive rate of inhalational allergens group was higher. Conclusions: The main allergen of asthma children in Changchun was mite, followed by cat fur, dog fur, grass, mould, beef and lamb
and milk. There was certain difference between preschool-age and school-age children, and no difference between sexes. Keywords: asthma in children; allergen; specific IgE A05-40 Bronchial basement membrane thickening and increased laminin deposition in children with chronic obstructive respiratory diseases L. Honkov ˇ a´ 1 , K. Berankov ´ a´ 1 , J. Uhl´ık2 , P. Pohunek1 . 1 Department of Paediatrics, 2nd Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czech Republic; 2 Department of Histology and Embryology, 2nd Faculty of Medicine, Charles University, Prague, Czech Republic Aim: The aim of the study was to compare histological changes of bronchial mucosa in children with bronchial asthma (AB), cystic fibrosis (CF), primary ciliary dyskinesia (PCD), and healthy subjects and to decide whether these changes are specific for AB or they may be present in other chronic obstructive respiratory diseases as well. Methods: Thirty subjects, aged 4 to 19 years (10y5m ±3.52), undergoing bronchoscopic examination for various clinical reasons (e.g. chronic cough) were included in the study. There were 6 children (8y8m ±2.06) in the control group, 14 with AB (9y10m ±1.7), 5 with CF (11y1m ±3.11), and 5 children with PCD (12y0m ±7.22). An endobronchial biopsy was taken from each patient. We studied the thickness of the basement membrane (BM) and the laminin-positive layer of the BM by light microscopy, using histological and immunohistochemical staining. Results: We found significant BM thickening (7.21±1.09 vs. 4.33±0.43, p < 0.01) and significant increase in the width of the laminin-positive layer (2.69±0.41 vs. 1.78±0.17, p < 0.01) in children with chronic obstructive respiratory diseases compared to controls. We did not find any significant differences between AB, CF and PCD groups. Conclusion: Significant increase in the thickness of bronchial basement membrane and its laminin-positive layer can be observed in children with chronic respiratory diseases. This finding is not specific for AB, similar changes are observed in children with PCD and CF. Supported by GAUK 340911 and IGA MZ NT/11444. A06-178 CD4+ memory T cells numbers in asthmatic mice and normal mice H.-J. Cheng1 , Y. Li1 , L. Liu1 , H. Cai2 , W.-X. Li1 . 1 First Hospital of Jilin University, Changchun, China; 2 Central Hospital of Changchun City Clinical laboratory, Changchun, China Objective: To understand the numbers of CD4+ memory T cells in asthmatic and normal mice. Method: Construct bronchial asthma models with female BALB/c mice. Mice were sacrificed at given time after the last challenge, and their spleens and lungs were made into single cell suspension. We detected the ratio of CD4+ memory T cells which membrane surface markers are CD3+CD4+CD44HighCD62L− by using flow cytometry. Results: In normal control mice, the ratios of CD4+ memory T cells in CD4+ T cells in spleens and lungs were 20.48% and 11.32%, respectively, while in asthmatic mice (48 hours after the last challenge), they were much higher with 30.215% and 45.545%, respectively; the ratios of CD4+ memory T cells in total cells in spleens and lungs in normal control mice were 5.41% and 3.38%, while in asthmatic mice, they were 8.545% and 9.185%, respectively. The ratios of CD4+ memory T cells in total cells reached peak value with 11.46% in spleens and 9.77% in lungs 1 week after the last challenge, while the ratios of CD4+ memory T cells in CD4+ T cells reached peak value with over 30% on the 3rd day in spleens and 50% on the 5th day in lungs. In both groups, no matter in spleens or lungs, CD4+ T cells mainly consisted of CD44LowCD62L+
Posters / Paediatric Respiratory Reviews 14S2 (2013) S55–S85
the paucity of evidence-based data. Consensus guidelines for the preoperative management of asthmatic children are needed. A03-17 Alveolar nitric oxide contribution to assess asthma control 1
2
2
1
2
O. Sardon , P. Corcuera , A. Aldasoro , J. Korta , J. Mintegui , J.I. Emparanza3 , E.G. Perez ´ Yarza1 . 1 Department of Pediatrics, Division of Respiratory Medicine, University Hospital Donostia; Medical School (U.D. San Sebastian), University of the Basque Country, Department of Pediatrics, San Sebastian, Spain; 2 Department of Pediatrics, Division of Respiratory Medicine, University Hospital Donostia, San Sebastian, Spain; 3 University Donostia Hospital Epidemiology Unit (CIBER-ESP), San Sebastian, Spain Objective: Alveolar nitric oxide measurement to study the association among clinical symptoms, lung function and nitric oxide (NO) in asthmatic children. Materials and Methods: A cross-sectional study with prospective data collection was performed in healthy and asthmatic children aged between 6–16 years old. Exhaled NO at multiple flows (50, 100 and 200 ml/s) was carried out by CLD88sp® analyzer. According to the two-compartment model, alveolar NO concentration (CANO ) and maximum airway NO flux (J awNO ) were calculated. In asthmatic patients, a questionnaire for asthma control (CAN) was completed and a forced spirometry was performed. Quantitative variables were FENO,50 , CANO , J awNO , CAN, FEV1 , FVC, FEV1 /FVC and FEF25–75 . The association among FENO,50 , CANO , J awNO , FEV1 and CAN was studied using Spearman’s rho and the degree of agreement for asthma control was studied using Cohen’s kappa. Results: We studied 162 children; 49 were healthy (group 1), 23 were asthmatic without treatment (group 2), 62 were asthmatic treated with inhaled corticosteroid (ICS) (group 3) and 18 were asthmatic treated with long-acting b2 -agonist and ICS (group 4). CANO (ppb) was 2.3 (0.1–6.7); 3 (0.2–9.2); 2.45 (0.1–24) and 2.1 (0.5–7.3) respectively. J awNO (pl/s) was 540 (98.33–1983); 2356.67 (120–6110); 1445.83 (156–11805) and 1282.5 (250–5636) respectively. The degree of agreement between FENO,50 and J awNO was substantial in all groups. No agreement was found to establish the degree of asthma control among the other variables. Conclusions: The results for CANO and J awNO in healthy children were similar to the findings of other studies published. There was no agreement among the three measurement instruments analyzed to assess the degree of asthma control. A04-180 Analysis of allergen in children with asthma in Changchun city H. Cheng, L. Liu, Y.-N. Li, H.-J. Cheng. First Hospital of Jilin University, Changchun, China Objective: To explore the distribution and character of allergens in children with asthma in Changchun. Method: 610 cases of asthma patients (3–14years old) who admitted to the pediatric pulmonary department of Jilin University in 2006– 2011 were detected the concentration of specific IgE by Allergy Screen method. Results: 290 in 610 got positive results in the test of 14 allergens, the total positive rate was 47.54%. As to the common allergens the positive rate in mites was the highest: 21.31%. Close after that was cat fur, dog fur and grass: 20%. Mold, beef and lamb, and milk also took a good part of it: 13%, 11% and 8%. Among the inhale groups, preschool-age children and school-age children had little difference (P > 0.05). Among the food allergen groups, infants in the rate of allergen would be higher (P > 0.01). The positive rate of allergen of preschool-age children had no difference between inhalational and food allergens groups, while in school-age children, the positive rate of inhalational allergens group was higher. Conclusions: The main allergen of asthma children in Changchun was mite, followed by cat fur, dog fur, grass, mould, beef and lamb
and milk. There was certain difference between preschool-age and school-age children, and no difference between sexes. Keywords: asthma in children; allergen; specific IgE A05-40 Bronchial basement membrane thickening and increased laminin deposition in children with chronic obstructive respiratory diseases L. Honkov ˇ a´ 1 , K. Berankov ´ a´ 1 , J. Uhl´ık2 , P. Pohunek1 . 1 Department of Paediatrics, 2nd Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czech Republic; 2 Department of Histology and Embryology, 2nd Faculty of Medicine, Charles University, Prague, Czech Republic Aim: The aim of the study was to compare histological changes of bronchial mucosa in children with bronchial asthma (AB), cystic fibrosis (CF), primary ciliary dyskinesia (PCD), and healthy subjects and to decide whether these changes are specific for AB or they may be present in other chronic obstructive respiratory diseases as well. Methods: Thirty subjects, aged 4 to 19 years (10y5m ±3.52), undergoing bronchoscopic examination for various clinical reasons (e.g. chronic cough) were included in the study. There were 6 children (8y8m ±2.06) in the control group, 14 with AB (9y10m ±1.7), 5 with CF (11y1m ±3.11), and 5 children with PCD (12y0m ±7.22). An endobronchial biopsy was taken from each patient. We studied the thickness of the basement membrane (BM) and the laminin-positive layer of the BM by light microscopy, using histological and immunohistochemical staining. Results: We found significant BM thickening (7.21±1.09 vs. 4.33±0.43, p < 0.01) and significant increase in the width of the laminin-positive layer (2.69±0.41 vs. 1.78±0.17, p < 0.01) in children with chronic obstructive respiratory diseases compared to controls. We did not find any significant differences between AB, CF and PCD groups. Conclusion: Significant increase in the thickness of bronchial basement membrane and its laminin-positive layer can be observed in children with chronic respiratory diseases. This finding is not specific for AB, similar changes are observed in children with PCD and CF. Supported by GAUK 340911 and IGA MZ NT/11444. A06-178 CD4+ memory T cells numbers in asthmatic mice and normal mice H.-J. Cheng1 , Y. Li1 , L. Liu1 , H. Cai2 , W.-X. Li1 . 1 First Hospital of Jilin University, Changchun, China; 2 Central Hospital of Changchun City Clinical laboratory, Changchun, China Objective: To understand the numbers of CD4+ memory T cells in asthmatic and normal mice. Method: Construct bronchial asthma models with female BALB/c mice. Mice were sacrificed at given time after the last challenge, and their spleens and lungs were made into single cell suspension. We detected the ratio of CD4+ memory T cells which membrane surface markers are CD3+CD4+CD44HighCD62L− by using flow cytometry. Results: In normal control mice, the ratios of CD4+ memory T cells in CD4+ T cells in spleens and lungs were 20.48% and 11.32%, respectively, while in asthmatic mice (48 hours after the last challenge), they were much higher with 30.215% and 45.545%, respectively; the ratios of CD4+ memory T cells in total cells in spleens and lungs in normal control mice were 5.41% and 3.38%, while in asthmatic mice, they were 8.545% and 9.185%, respectively. The ratios of CD4+ memory T cells in total cells reached peak value with 11.46% in spleens and 9.77% in lungs 1 week after the last challenge, while the ratios of CD4+ memory T cells in CD4+ T cells reached peak value with over 30% on the 3rd day in spleens and 50% on the 5th day in lungs. In both groups, no matter in spleens or lungs, CD4+ T cells mainly consisted of CD44LowCD62L+