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CARCINOGEN-RELATED CYP1A1 AND GSTM1 GENETIC POLYMORPHISMS IN ORAL CANCER AND ORAL POTENTIALLY MALIGNANT DISORDERS
ABSTRACTS
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Objective: To evaluate genotypic characteristics of patients with oral cancer (OC) in order to develop a statistical risk prediction model for Argentine populations, and to improve their prevention, treatment, and follow-up care. Methods: A case-control study of adult patients, both sexes, attended by spontaneous demand, was carried out. Exclusion criteria: Treatment with corticoids or chemotherapies, systemic diseases, chronic alcoholism, drug consumption, and cancers from other sites (protocol approved by the ethics committee: 1379). The prognostic factors were assessed by logistic regression and Irwin-Fisher test; it was fixed P < .05 for statistical significance. Results: p53 R72P: Significant associations between controls and genotype GG; and between OC with the GC and CC genotypes. XPDLys751 Gln showed a significant association between OC and the polymorphic variant. There was a significant association between the genotype XRCC3 Thr241 Met and its polymorphic variant. Conclusions: Polymorphisms play different roles in the development of cancer in different populations; therefore, it is possible that the variations are due to geographic and racial differences plus other risk factors. This work shows our preliminary results that should be confirmed in studies that include more patients belonging to different areas of Argentina.
CARCINOGEN-RELATED CYP1A1 AND GSTM1 GENETIC POLYMORPHISMS IN ORAL CANCER AND ORAL POTENTIALLY MALIGNANT DISORDERS. VICTIRUANO ANDRES CARRICA, IRAZUZTA GRACIANA, UNAMUNO VICTORIA, PANICO RENE, GALINDEZ COSTA MARIA FERNANDA, JOSE L. BARRA and, ANA M. ZARATE Objective: To assess carcinogen related CYP1A1 and GSTM1 genetic polymorphisms in oral cancer (OC) and oral potentially malignant disorders (OPMD). Study Design: One hundred smokers were studied: OC = 30, OPMD = 45 (leukoplakia and oral lichen planus) and 25 controls (Con) by means of biopsy and exfoliative cytology (EC) of the lesion and clinically normal mucosa of a contralateral site. After extracting DNA from EC, polymorphisms study of CYP1A1 using polymerase chain reaction (PCR)-retriction fragment length polymorphism (RFLP) and GSTM1 using conventional PCR were done. Results: The CYP1A1 polymorphisms found were (m1/ m1), (m1/m2) and (m2/m2). All control showed m1/m1 (wildtype), whereas 80% of OC had (m1/m2) and (m2/m2) genotype. 68% of the OPMD displayed m1/m1. Regarding GSTM1, (no null) wild-type and (null) genotypes were studied; 75% of control had no null, 73% of OC showed null and in OPMD there were no significant differences in the null and no null appearance. Conclusions: The modified genotypes of CYP1A1 and GSTM1 could indicate a predisposition for oral cancer development.
MSH2 EXPRESSION IN ORAL SQUAMOUS CELL CARCINOMA. NATALIA DA SILVA MIGUEL, PATRICIA PIMENTEL DE BARROS, CELINA FAIG LIMA CARTA, JOSE FRANCISCO DE SALES CHAGAS, CELSO MULLER BANDEIRA, JANETE DIAS ALMEIDA and, MONICA GHISLAINE OLIVEIRA ALVES
OOOO January 2020 Objective: The objective of the present work was to evaluate the DNA repair gene MSH2 expression in oral squamous cell carcinoma (OSCC). Study Design: The carcinoma group was composed of 32 OSCC samples and the control group by 15 samples of tissue borders of benign lesions removed from the oral cavity of patients equivalent in age, sex, and smoking habits to the patients of OSCC group. Gene expression was assessed by quantitative reverse transcription polymerase chain reaction (RT-qPCR). In addition, clinical data, such as age, amount of cigarettes per day, time of tobacco consumption, smoking history (pack-years), degree of nicotine dependence, risk of alcohol use, and neoplasm staging were evaluated. The groups were compared using the Student t test and the correlation of the expression levels and clinical data were explored using the Pearson correlation coefficient, with P < .05 indicating significant difference. Results: It was observed higher prevalence of males, with a mean age in years § SD of 57.44 § 13.32. The MSH2 gene was downregulated (1.13 fold decrease) in the OSCC group compared to the control with significant results (P = .0380). There was no significant correlation between MSH2 expression and the clinical variables studied. Conclusions: MSH2 expression in OSCC was reduced.
COMPARATIVE STUDY BETWEEN CRYOSURGERY AND CONVENTIONAL SURGERY IN THE TREATMENT OF ORAL MUCOCELES. CAMILA CARLA MARIA XIMENES DE OLIVEIRA, INGRID CARLA GUEDES DA SILVA, MARIANA DE MORAES CORREA PEREZ, SARAH RACHEL CAVALCANTE BEZERRA MELO, LUIZ ALCINO MONTEIRO GUEIROS, JAIR CARNEIRO LEAO and, ALESSANDRA DE ALBUQUERQUE TAVARES CARVALHO Objective: To compare trans- and postsurgical results of patients undergoing excision of lesions clinically compatible with oral mucoceles through 2 forms of treatment: Conventional surgery using scalpel and cryosurgery with liquid nitrogen. Study design: A randomized clinical trial was performed with 17 patients with a clinical diagnosis of oral mucoceles. Patients were divided into 2 groups: 7 patients of group 1 underwent surgical excision and 10 patients of group 2 (G2) had lesions treated by cryosurgery. The parameters used in the comparison were: Need for anesthesia; intensity of transoperative bleeding; need for suture; postoperative pain, and need for analgesic administration. Results: The variables transsurgical bleeding, need for anesthesia, and need for suturing are totally different procedures among the treatments, since patients of group 1 presents 100.0% of scarce bleeding, 100.0% needed anesthesia and 100, 0% needed suture while in group 2 all these percentage was zero. Conclusions: Cryosurgery is an effective method for the treatment of oral mucoceles, and can offer considerable advantages because it is a simple and painless technique, in which sutures are unnecessary, as the bleeding is nonexistent.
CLINICOPATHOLOGIC PROFILE OF NONNEOPLASTIC AND NEOPLASTIC LESIONS OF SALIVARY GLANDS. JULIANNA MENDES ^ DO SANTOS, MANOEL SALES, JOSE WITTOR DE MACE PEREIRA DE LIMA and, MANUEL ANTONIO GORDONe NEZ NU
e154
Objective: To evaluate genotypic characteristics of patients with oral cancer (OC) in order to develop a statistical risk prediction model for Argentine populations, and to improve their prevention, treatment, and follow-up care. Methods: A case-control study of adult patients, both sexes, attended by spontaneous demand, was carried out. Exclusion criteria: Treatment with corticoids or chemotherapies, systemic diseases, chronic alcoholism, drug consumption, and cancers from other sites (protocol approved by the ethics committee: 1379). The prognostic factors were assessed by logistic regression and Irwin-Fisher test; it was fixed P < .05 for statistical significance. Results: p53 R72P: Significant associations between controls and genotype GG; and between OC with the GC and CC genotypes. XPDLys751 Gln showed a significant association between OC and the polymorphic variant. There was a significant association between the genotype XRCC3 Thr241 Met and its polymorphic variant. Conclusions: Polymorphisms play different roles in the development of cancer in different populations; therefore, it is possible that the variations are due to geographic and racial differences plus other risk factors. This work shows our preliminary results that should be confirmed in studies that include more patients belonging to different areas of Argentina.
CARCINOGEN-RELATED CYP1A1 AND GSTM1 GENETIC POLYMORPHISMS IN ORAL CANCER AND ORAL POTENTIALLY MALIGNANT DISORDERS. VICTIRUANO ANDRES CARRICA, IRAZUZTA GRACIANA, UNAMUNO VICTORIA, PANICO RENE, GALINDEZ COSTA MARIA FERNANDA, JOSE L. BARRA and, ANA M. ZARATE Objective: To assess carcinogen related CYP1A1 and GSTM1 genetic polymorphisms in oral cancer (OC) and oral potentially malignant disorders (OPMD). Study Design: One hundred smokers were studied: OC = 30, OPMD = 45 (leukoplakia and oral lichen planus) and 25 controls (Con) by means of biopsy and exfoliative cytology (EC) of the lesion and clinically normal mucosa of a contralateral site. After extracting DNA from EC, polymorphisms study of CYP1A1 using polymerase chain reaction (PCR)-retriction fragment length polymorphism (RFLP) and GSTM1 using conventional PCR were done. Results: The CYP1A1 polymorphisms found were (m1/ m1), (m1/m2) and (m2/m2). All control showed m1/m1 (wildtype), whereas 80% of OC had (m1/m2) and (m2/m2) genotype. 68% of the OPMD displayed m1/m1. Regarding GSTM1, (no null) wild-type and (null) genotypes were studied; 75% of control had no null, 73% of OC showed null and in OPMD there were no significant differences in the null and no null appearance. Conclusions: The modified genotypes of CYP1A1 and GSTM1 could indicate a predisposition for oral cancer development.
MSH2 EXPRESSION IN ORAL SQUAMOUS CELL CARCINOMA. NATALIA DA SILVA MIGUEL, PATRICIA PIMENTEL DE BARROS, CELINA FAIG LIMA CARTA, JOSE FRANCISCO DE SALES CHAGAS, CELSO MULLER BANDEIRA, JANETE DIAS ALMEIDA and, MONICA GHISLAINE OLIVEIRA ALVES
OOOO January 2020 Objective: The objective of the present work was to evaluate the DNA repair gene MSH2 expression in oral squamous cell carcinoma (OSCC). Study Design: The carcinoma group was composed of 32 OSCC samples and the control group by 15 samples of tissue borders of benign lesions removed from the oral cavity of patients equivalent in age, sex, and smoking habits to the patients of OSCC group. Gene expression was assessed by quantitative reverse transcription polymerase chain reaction (RT-qPCR). In addition, clinical data, such as age, amount of cigarettes per day, time of tobacco consumption, smoking history (pack-years), degree of nicotine dependence, risk of alcohol use, and neoplasm staging were evaluated. The groups were compared using the Student t test and the correlation of the expression levels and clinical data were explored using the Pearson correlation coefficient, with P < .05 indicating significant difference. Results: It was observed higher prevalence of males, with a mean age in years § SD of 57.44 § 13.32. The MSH2 gene was downregulated (1.13 fold decrease) in the OSCC group compared to the control with significant results (P = .0380). There was no significant correlation between MSH2 expression and the clinical variables studied. Conclusions: MSH2 expression in OSCC was reduced.
COMPARATIVE STUDY BETWEEN CRYOSURGERY AND CONVENTIONAL SURGERY IN THE TREATMENT OF ORAL MUCOCELES. CAMILA CARLA MARIA XIMENES DE OLIVEIRA, INGRID CARLA GUEDES DA SILVA, MARIANA DE MORAES CORREA PEREZ, SARAH RACHEL CAVALCANTE BEZERRA MELO, LUIZ ALCINO MONTEIRO GUEIROS, JAIR CARNEIRO LEAO and, ALESSANDRA DE ALBUQUERQUE TAVARES CARVALHO Objective: To compare trans- and postsurgical results of patients undergoing excision of lesions clinically compatible with oral mucoceles through 2 forms of treatment: Conventional surgery using scalpel and cryosurgery with liquid nitrogen. Study design: A randomized clinical trial was performed with 17 patients with a clinical diagnosis of oral mucoceles. Patients were divided into 2 groups: 7 patients of group 1 underwent surgical excision and 10 patients of group 2 (G2) had lesions treated by cryosurgery. The parameters used in the comparison were: Need for anesthesia; intensity of transoperative bleeding; need for suture; postoperative pain, and need for analgesic administration. Results: The variables transsurgical bleeding, need for anesthesia, and need for suturing are totally different procedures among the treatments, since patients of group 1 presents 100.0% of scarce bleeding, 100.0% needed anesthesia and 100, 0% needed suture while in group 2 all these percentage was zero. Conclusions: Cryosurgery is an effective method for the treatment of oral mucoceles, and can offer considerable advantages because it is a simple and painless technique, in which sutures are unnecessary, as the bleeding is nonexistent.
CLINICOPATHOLOGIC PROFILE OF NONNEOPLASTIC AND NEOPLASTIC LESIONS OF SALIVARY GLANDS. JULIANNA MENDES ^ DO SANTOS, MANOEL SALES, JOSE WITTOR DE MACE PEREIRA DE LIMA and, MANUEL ANTONIO GORDONe NEZ NU