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Comparison of glycopeptide in lung cancer tissues of different histological types
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Characterization of a malignant phenotype-associated cell surface glycoprotein common to various human tumor cells and preferentially exprwed on adenocarcinoma of the lung. Motte P, Takahashi H, Ozturk M, Wilson BE, Wands JR. Molecular HepalologyLoborarory,CancerCenter,Massach~ettsGeneralHospital. Charlestown, MA 02129. Cancer Res 1989;49:1349-56.
Since both the liver and lung are derived from the endoderm, common antigens may appear on both tissues during malignant transformation. In an attempt to delineate cell surface alterations associated with the neoplastic transformation of these tissues, we have produced a library of monoclonal antibodies against a human hepatoma cell line termed FOCUS.Oneofthesemonoclonalantibodies,designatedAF-IO,recognized an antigen preferentially expressed on human lung adenocarcinema cells, both in vitro and in viva. This antigen has been character-
izedusing Westernimmunoblotanalysisandimmunoprecipitationfrom surface-iodinated or metabolically labeled cells. The mature antigen is a cell surface glycoprotein with a core polypeptide with a molecular weight of 75,000 bearing N-glycosylation units. This protein migrates in sodium dodecyl sulfate-polyacrylamide gel electrophoresis with an apparent molecular weight of lOO,OoO-115,000in reducing conditions and M(r) ll5,000-130,000 in nonreducing conditions. The epitope recognized by monoclonal antibody AF- IO is borne by the core protein. Thisantigenisshedfromthecellsurfaceandwasidentifiedintheculture supernatant from lung adenocarcinoma cell lines. Studies of the biodistribution of the AF- 10 antigen showed that it was also expressed at low levels in normal human small intestine and kidney. The AF-10 monoclonal antibody may be useful for the study of the antigen expression between normal lung and the transformed phenotype.
Pathology Cytologic presentation of malignant mesotbelioma in pleural effusions. Pedio G, Landolt-Weber U. Instiwe of Pathology, Deparmenr of Cytology. University of Zurich, CH-8091 Zurich. Exp Cell Biol 1988;56:211-6. I.601 Pleural effusions were found to be malignant between 1976 and 1987. Among these were 26 (I .6% of the malignant effusions) mesothelioma. Only 2 cases showed pronounced cytologic features that made a definite diagnosis possible on cytologic criteria alone. In 20 cases diagnosis of mesothelioma was strongly suggested by the patients’ history and cytology of the effusion was compatible with mesothelioma. Intheother4casesspecialexaminations(histo-andimmunohistochemistry, electron microscopy) led to the final diagnosis. The cytologic features of mesothelioma and other examination techniques, needed to resolve the differential diagnosis of mesothelioma versus other neoplasm in pleural effusions, are discussed. Incidence of lung cancer by histological type from a populationhased registry. Anton-Culver H, Culver BD, Kumsaki T, Osann KE, Lee JB. Department ofCommunityandEnvironmen~alMedicine, UniversityofCalifornia, Irvine, CA 92717. Cancer Res 1988;48:6580-3.
Using data from a population-based registry, the Cancer Surveillance Program of Grange County, we examined patterns in lung cancer incidence by histological type for 1984 in Grange County, CA. Ageadjusted incidence rates per 100,ooO population are 66.4 for men and 34.1 forwomen.Compared to 1983ratesforwhitesfrom allSEERweas combined, Orange County incidence rates are lower for men but equal for women. Squamous cell carcinoma incidence shows a strong male predominance [male/female 3.4; 95% confidence interval = (2.6,4.4)1, whereas the male/ female incidence ratios for adenocarcinoma [male/ female 1.4; 95% confidence interval = (1.1, 1.8)) and small cell carcinoma [male/female = 1.8; 95% confidence interval = 1.3,2.4)1 are closertounity.Smokinghabitswereabstractedfrommedicalrecordsfor 79% of cases. Only 8% of lung cancer cases (5% of men and 12% of women) with known smoking habits are nonsmokers. Adenocarcinoma is the most common cell type among women smokers and nonsmokers, while squamous cell carcinoma predominates in both male smokers and nonsmokers. Cases who smoked were younger at diagnosis than nonsmokers (P
Occupalional
and
Environmental
Health,
Yaharanishi-ku,
Kita!w.shu-City, Fukuoka 807. Biochem Med Metab 1988;40:253-9.
The glycopeptide was studied in the pulmonary cancer tissues of three different histological types including squamous cell carcinoma, undifferentiated cell carcinoma, and adenocarcinoma. The glycopcptide prepartion was madeby digesting the lung cancer tissue with pmteolytic enzymes followed by removal of enzymes and undigested protein. The COmpariwn of the relative proportion of the glycopeptide, which migrated toward the anode slower than hyaluronic acid, and was stained with both alcian blue and PAS, and contained higher sialic acid, was carried out among three different histologic types of lung cancer. This glycopeptide was highest in adenocarcinoma, followed by undifferentiated cell carcinoma, and was lowest in squamous cell carcinoma.
Lung cancer cell type and mining exposure. DaviesJCA,LandauSP,GodsmithC.NationalCenrreforOccupafional Health, Deparrment ofNational Health and Population Developmenr, Johannesburg. S Afr Med J 1988;74:607-IO.
It is frequently stated that the most common primary lung cancer cell type in asbestos-exposed persons is adenocarcinoma. Recently a contrary opinion has been expressed. This study compares the cell types recorded among deceased South African miners whose lungs were examinedatthe National Centre forOccupational Health. It supports the opinion that adenocarcinoma is not associated with asbestos exposure. From the large database available, there is no evidence of a consistent association between any particular cell type and specific mining exposure. False positive diagnosis of broncbogenic carcinoma based on bronchoscopic brushing and sputum cytology. A surgical point of view. Lodi M, Susa A, Cavallini G. Isfiuto di Malatrie dell’Appararo Respiralorio e Tisiologia, Universira di Ferrara. Ferrara. Ital J Surg Sci 1988;18:385-8. The problem of false positive diagnosis of bronchogenic carcinoma basedonbronchoscopichmshingandsputumcytologyisanalysed. 1071 patients were reviewed, 230 of whom underwent ‘radical’ resection. Diagnoses obtained from bronchoscopic brushing, sputum cytology and microscopic examination of surgical specimens were compared. The statistical analysis of the obtained results showed that ‘false positives’ should be thoroughly evaluated. The concept of cancer in situ and the hypothesis of a multi-step evolution of bronchogenic carcinoma are briefly discussed.
Malignant change in a benign pulmonary bamartoma. Basile A, Gregoris A. Antoci B, Romanelli M. Divisione di Medicine Interna, 33078 San Vito al Tagliamento. norax 1989;44:232-3. Sarcomatous hansformation in a long-standing, clinically silent hamartoma is reported. Malignant change occurred shortly after wettion, suggesting that surgical manipulation may have played a part.
Characterization of a malignant phenotype-associated cell surface glycoprotein common to various human tumor cells and preferentially exprwed on adenocarcinoma of the lung. Motte P, Takahashi H, Ozturk M, Wilson BE, Wands JR. Molecular HepalologyLoborarory,CancerCenter,Massach~ettsGeneralHospital. Charlestown, MA 02129. Cancer Res 1989;49:1349-56.
Since both the liver and lung are derived from the endoderm, common antigens may appear on both tissues during malignant transformation. In an attempt to delineate cell surface alterations associated with the neoplastic transformation of these tissues, we have produced a library of monoclonal antibodies against a human hepatoma cell line termed FOCUS.Oneofthesemonoclonalantibodies,designatedAF-IO,recognized an antigen preferentially expressed on human lung adenocarcinema cells, both in vitro and in viva. This antigen has been character-
izedusing Westernimmunoblotanalysisandimmunoprecipitationfrom surface-iodinated or metabolically labeled cells. The mature antigen is a cell surface glycoprotein with a core polypeptide with a molecular weight of 75,000 bearing N-glycosylation units. This protein migrates in sodium dodecyl sulfate-polyacrylamide gel electrophoresis with an apparent molecular weight of lOO,OoO-115,000in reducing conditions and M(r) ll5,000-130,000 in nonreducing conditions. The epitope recognized by monoclonal antibody AF- IO is borne by the core protein. Thisantigenisshedfromthecellsurfaceandwasidentifiedintheculture supernatant from lung adenocarcinoma cell lines. Studies of the biodistribution of the AF- 10 antigen showed that it was also expressed at low levels in normal human small intestine and kidney. The AF-10 monoclonal antibody may be useful for the study of the antigen expression between normal lung and the transformed phenotype.
Pathology Cytologic presentation of malignant mesotbelioma in pleural effusions. Pedio G, Landolt-Weber U. Instiwe of Pathology, Deparmenr of Cytology. University of Zurich, CH-8091 Zurich. Exp Cell Biol 1988;56:211-6. I.601 Pleural effusions were found to be malignant between 1976 and 1987. Among these were 26 (I .6% of the malignant effusions) mesothelioma. Only 2 cases showed pronounced cytologic features that made a definite diagnosis possible on cytologic criteria alone. In 20 cases diagnosis of mesothelioma was strongly suggested by the patients’ history and cytology of the effusion was compatible with mesothelioma. Intheother4casesspecialexaminations(histo-andimmunohistochemistry, electron microscopy) led to the final diagnosis. The cytologic features of mesothelioma and other examination techniques, needed to resolve the differential diagnosis of mesothelioma versus other neoplasm in pleural effusions, are discussed. Incidence of lung cancer by histological type from a populationhased registry. Anton-Culver H, Culver BD, Kumsaki T, Osann KE, Lee JB. Department ofCommunityandEnvironmen~alMedicine, UniversityofCalifornia, Irvine, CA 92717. Cancer Res 1988;48:6580-3.
Using data from a population-based registry, the Cancer Surveillance Program of Grange County, we examined patterns in lung cancer incidence by histological type for 1984 in Grange County, CA. Ageadjusted incidence rates per 100,ooO population are 66.4 for men and 34.1 forwomen.Compared to 1983ratesforwhitesfrom allSEERweas combined, Orange County incidence rates are lower for men but equal for women. Squamous cell carcinoma incidence shows a strong male predominance [male/female 3.4; 95% confidence interval = (2.6,4.4)1, whereas the male/ female incidence ratios for adenocarcinoma [male/ female 1.4; 95% confidence interval = (1.1, 1.8)) and small cell carcinoma [male/female = 1.8; 95% confidence interval = 1.3,2.4)1 are closertounity.Smokinghabitswereabstractedfrommedicalrecordsfor 79% of cases. Only 8% of lung cancer cases (5% of men and 12% of women) with known smoking habits are nonsmokers. Adenocarcinoma is the most common cell type among women smokers and nonsmokers, while squamous cell carcinoma predominates in both male smokers and nonsmokers. Cases who smoked were younger at diagnosis than nonsmokers (P
Occupalional
and
Environmental
Health,
Yaharanishi-ku,
Kita!w.shu-City, Fukuoka 807. Biochem Med Metab 1988;40:253-9.
The glycopeptide was studied in the pulmonary cancer tissues of three different histological types including squamous cell carcinoma, undifferentiated cell carcinoma, and adenocarcinoma. The glycopcptide prepartion was madeby digesting the lung cancer tissue with pmteolytic enzymes followed by removal of enzymes and undigested protein. The COmpariwn of the relative proportion of the glycopeptide, which migrated toward the anode slower than hyaluronic acid, and was stained with both alcian blue and PAS, and contained higher sialic acid, was carried out among three different histologic types of lung cancer. This glycopeptide was highest in adenocarcinoma, followed by undifferentiated cell carcinoma, and was lowest in squamous cell carcinoma.
Lung cancer cell type and mining exposure. DaviesJCA,LandauSP,GodsmithC.NationalCenrreforOccupafional Health, Deparrment ofNational Health and Population Developmenr, Johannesburg. S Afr Med J 1988;74:607-IO.
It is frequently stated that the most common primary lung cancer cell type in asbestos-exposed persons is adenocarcinoma. Recently a contrary opinion has been expressed. This study compares the cell types recorded among deceased South African miners whose lungs were examinedatthe National Centre forOccupational Health. It supports the opinion that adenocarcinoma is not associated with asbestos exposure. From the large database available, there is no evidence of a consistent association between any particular cell type and specific mining exposure. False positive diagnosis of broncbogenic carcinoma based on bronchoscopic brushing and sputum cytology. A surgical point of view. Lodi M, Susa A, Cavallini G. Isfiuto di Malatrie dell’Appararo Respiralorio e Tisiologia, Universira di Ferrara. Ferrara. Ital J Surg Sci 1988;18:385-8. The problem of false positive diagnosis of bronchogenic carcinoma basedonbronchoscopichmshingandsputumcytologyisanalysed. 1071 patients were reviewed, 230 of whom underwent ‘radical’ resection. Diagnoses obtained from bronchoscopic brushing, sputum cytology and microscopic examination of surgical specimens were compared. The statistical analysis of the obtained results showed that ‘false positives’ should be thoroughly evaluated. The concept of cancer in situ and the hypothesis of a multi-step evolution of bronchogenic carcinoma are briefly discussed.
Malignant change in a benign pulmonary bamartoma. Basile A, Gregoris A. Antoci B, Romanelli M. Divisione di Medicine Interna, 33078 San Vito al Tagliamento. norax 1989;44:232-3. Sarcomatous hansformation in a long-standing, clinically silent hamartoma is reported. Malignant change occurred shortly after wettion, suggesting that surgical manipulation may have played a part.