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Culture of animal cells: A manual of basic technique
JOURNAL OF IMMUNOlO6ICAL METHODS ELSEVIER
Journal
of Immunological
Methods
183 (1995) 291-292
Book reviews Freshney, R.I., Culture of Animal Cells: A Manual of Basic Technique, 3rd edn., 496 pp., WileyLiss, New York, 1993. USD 69.95, ISBN 0-47158966-7 This is a revised and expanded version of the widely known manual of techniques for culturing mammalian cells. It provides the necessary information for tissue culture work, from biology of the cultured cell, laboratory design and equipment, and the principles of aseptic technique, through safety precautions, media preparation and sterilization, tissue disaggregation and primary culture, culture maintenance, cloning and selection of cell strains, to the physical methods of cell separation, induction of differentiation, quantitation, viability and cytotoxicity measure-
Ceriani, R.L. (Ed.), Antigen and Antibody Molecular Engineering in Breast Cancer Diagnosis and Treatment, xii + 222 pp. Plenum Press, New York, 1994. USD 75.00, ISBN o-306-44720-7. This book contains proceedings of the 5th International Workshop on Breast Cancer Research and Immunology, held November 16-17, 1992, in San Francisco, CA, with the participation of biomedical scientists from the USA, UK, Canada, Israel, Australia, Japan, Italy, and Finland. The objective of the conference was to facilitate the exchange of recent findings and ideas in the immunological research on breast cancer. 0022-1759/95/$09.50 0 1995 Elsevier SSDI 0022-1759(95)00062-3
Science
ments, tumour tissue culture, and other special techniques. The text is accompanied by numerous photographs, diagrams, and graphs. Appended are a list of required reagents, a list of the suppliers of materials, a glossary of terms, a list of references, and a subject index. Immunologists may be interested to know that the author, a member of the Department of Medical Oncology at the University of Glasgow, coedited the book on the Culture of Hematopoietic Cells, published by Wiley-Liss in 1994. In view of the abundant information it contains, this volume can be recommended for use as a reference textbook for biomedical researchers. F. BOREK
The reports herein deal mainly with the immunologically significant substance present on the surface of cancer cells, breast cancer mucin (BCM). This antigen, recognized by most monoclonal antibodies against human breast carcinomas, is secreted by the cells and can be detected in the sera of such patients. The opening paper elucidates the glycoprotein structure of BCM and identifies its peptide epitopes, which also form the subject of the next report. Some of the contributions examine the evidence for the functions of BCM in modulating signal transmission through the cell membrane, in carbohydrate-mediated cell adhesion, and as a prognostic marker in predicting tumour recurrence. Other authors evaluate
B.V. All rights reserved
Journal
of Immunological
Methods
183 (1995) 291-292
Book reviews Freshney, R.I., Culture of Animal Cells: A Manual of Basic Technique, 3rd edn., 496 pp., WileyLiss, New York, 1993. USD 69.95, ISBN 0-47158966-7 This is a revised and expanded version of the widely known manual of techniques for culturing mammalian cells. It provides the necessary information for tissue culture work, from biology of the cultured cell, laboratory design and equipment, and the principles of aseptic technique, through safety precautions, media preparation and sterilization, tissue disaggregation and primary culture, culture maintenance, cloning and selection of cell strains, to the physical methods of cell separation, induction of differentiation, quantitation, viability and cytotoxicity measure-
Ceriani, R.L. (Ed.), Antigen and Antibody Molecular Engineering in Breast Cancer Diagnosis and Treatment, xii + 222 pp. Plenum Press, New York, 1994. USD 75.00, ISBN o-306-44720-7. This book contains proceedings of the 5th International Workshop on Breast Cancer Research and Immunology, held November 16-17, 1992, in San Francisco, CA, with the participation of biomedical scientists from the USA, UK, Canada, Israel, Australia, Japan, Italy, and Finland. The objective of the conference was to facilitate the exchange of recent findings and ideas in the immunological research on breast cancer. 0022-1759/95/$09.50 0 1995 Elsevier SSDI 0022-1759(95)00062-3
Science
ments, tumour tissue culture, and other special techniques. The text is accompanied by numerous photographs, diagrams, and graphs. Appended are a list of required reagents, a list of the suppliers of materials, a glossary of terms, a list of references, and a subject index. Immunologists may be interested to know that the author, a member of the Department of Medical Oncology at the University of Glasgow, coedited the book on the Culture of Hematopoietic Cells, published by Wiley-Liss in 1994. In view of the abundant information it contains, this volume can be recommended for use as a reference textbook for biomedical researchers. F. BOREK
The reports herein deal mainly with the immunologically significant substance present on the surface of cancer cells, breast cancer mucin (BCM). This antigen, recognized by most monoclonal antibodies against human breast carcinomas, is secreted by the cells and can be detected in the sera of such patients. The opening paper elucidates the glycoprotein structure of BCM and identifies its peptide epitopes, which also form the subject of the next report. Some of the contributions examine the evidence for the functions of BCM in modulating signal transmission through the cell membrane, in carbohydrate-mediated cell adhesion, and as a prognostic marker in predicting tumour recurrence. Other authors evaluate
B.V. All rights reserved