- Email: [email protected]
Cytological Investigation of Mammary Gland Tumours in the Queen and Bitch
352
ESVP/ECVP Proceedings 2010
143:4, 2010
P9
P10
HETEROTOPIC SALIVARY GLAND TISSUE IN THE RETROBULBAR SPACE IN A DOG L. Frgelecova, P. Fictum and M. Skoric University of Veterinary and Pharmaceutical Sciences, Czech Republic Introduction: A 10-year-old female German shepherd dog was presented with a retrobulbar mass compressing the eyeball. Histopathological examination showed differentiated seromucinous gland tissue, morphologically consistent with salivary gland. Materials and Methods: Biopsy samples were collected for microscopical examination, fixed in 10% neutral buffered formalin, dehydrated, embedded in paraffin wax, sectioned (4 mm) and stained with HE. For comparison, samples of normal salivary gland, lachrymal gland and third eyelid gland were studied. Results: Microscopical examination of the retrobulbar mass revealed seromucinous tubuloalveolar glands with predominance of a mucinous component and presence of intercalated and striated ducts. In some parts of the parenchyma, cystic dilatation of alveoli and ducts was observed. There were no signs of neoplastic changes. Conclusions: A diagnosis of salivary gland heterotopia in the retrobulbar space was made.
CYTOLOGICAL INVESTIGATION OF MAMMARY GLAND TUMOURS IN THE QUEEN AND BITCH M. Militaru, E. Ciobotaru, G. Dinescu, A. Diaconescu, I. Ionascu and D. Cranganu Faculty of Veterinary Medicine, Romania Introduction: The role of cytological examination of mammary gland tumours is controversial. The aim of this study was to establish the degree of correlation between cytological and histopathological examination of such lesions. Materials and Methods: Mammary gland tumours from 39 queens and 88 bitches were investigated. The diagnosis was established by cytological (fine needle aspiration and imprint cytology stained by MGG) and histopathological (Masson’s trichrome stain) methods. Histopathological diagnoses were made according to the WHO classification. Results: The cytological criteria evaluated included morphology, presence of secretory and ductal epithelial cells, mesenchymal cells with and without metaplasia, the presence of inflammatory cells and the background content. The disparity in diagnosis was 7.7% of feline tumours and it was correlated with the presence of cellular disruption with free nuclei, the presence of an inflammatory process and the lack of clinical data. The disparity in diagnosis was 10.2% of canine tumours and it was correlated with the presence of an inflammatory process, stromal metaplasia and the structural complexity of the tumours. Conclusions: Cytological diagnosis of mammary gland tumours is more accurate in the queen than the bitch. In dogs, diagnostic cytology via fine needle aspiration has a limited value, but it is a useful method for determining lymph node metastasis.
P11
P12
POULTRY HEPATOPATHIES AND THEIR CORRELATION WITH FOOD SAFETY M. Militaru, L. Tudor, M.O. Ghimpeteanu, E. Ciobotaru and G. Dinescu Faculty of Veterinary Medicine, Romania Introduction: The nature of liver lesions and their correlation with the isolation of potentially pathogenic microorganisms can provide information about the general health status of a poultry flock and the potential danger to the health of consumers. The aim of this study was to evaluate the association between different types of hepatopathy diagnosed in rejected organs with the presence of some microorganisms. Materials and Methods: Livers (n 5 411) from young abattoirslaughtered poultry were investigated. Cytological (May Gr€ unwald Giemsa stain), histological (Masson’s trichrome and Congo red stains) and microbiological examinations were performed on 22 livers considered representative of different gross lesions. Results: The two major types of lesion were non-specific hepatopathy with diffuse degenerative features associated with vascular reactions and multifocal miliary necrotic hepatitis. Cytologically, the degree of hepatocyte degeneration and the nature of the inflammatory infiltrates were investigated. Histologically, different types of lesions were described including hepatosis, miliary necrotic hepatitis, diffuse interstitial infiltration and amyloidosis. The most common microorganisms isolated were E. coli, Pseudomonas spp. and yeasts. Conclusions: 94.4% of the samples had non-specific hepatopathy and 5.6% had multifocal miliary necrotic hepatitis. Miliary necrotic hepatitis was associated with Pseudomonas spp. (5/7) and non-specific hepatopathy was correlated with isolation of E. coli (8/15), yeasts (8/15) and Enterococcus spp. (7/15).
OBSERVATIONS CONCERNING CADMIUM AS A POLLUTANT ELEMENT IN SOIL AND FORAGES AND ITS INFLUENCE ON ANIMALS IN THE SOUTHERN AREA OF BUCHAREST E. Mitranescu, M. Militaru, L. Tudor, E. Ciobotaru and F. Furnaris Faculty of Veterinary Medicine, Bucharest, Romania Introduction: Cadmium is one of the pollutant elements emitted in the eastern area of Bucharest. It may accumulate in the liver, kidney, heart and brain. Materials and Methods: Soil samples were collected from different depths and distances from the source of pollution, and from forage samples and organs collected from horses, pigs and ruminants. The cadmium concentration was analyzed by atomic absorption spectrophotometry. Animals were subjected to clinical examination followed by gross necropsy examination and microscopical evaluation of tissue sections. Results: Cadmium concentrations exceeded the maximum permitted limits by 2.3 times in soil samples collected from depths under 10 cm and at 200 m distance from the pollution source; by 3.57 times in forage samples; and in organs the limits were exceeded by nine times in horse liver and almost 40 times in horse kidney. High concentrations of cadmium were related to clinical signs (mainly digestive and respiratory) and to major lesions of the kidney and liver (nephritis and hepatosteatosis). Conclusion: The correlation between cadmium concentration in soil, plants and organs and the clinical and pathological findings is consistent with chronic cadmium toxicosis in animals in the eastern area of Bucharest.
ESVP/ECVP Proceedings 2010
143:4, 2010
P9
P10
HETEROTOPIC SALIVARY GLAND TISSUE IN THE RETROBULBAR SPACE IN A DOG L. Frgelecova, P. Fictum and M. Skoric University of Veterinary and Pharmaceutical Sciences, Czech Republic Introduction: A 10-year-old female German shepherd dog was presented with a retrobulbar mass compressing the eyeball. Histopathological examination showed differentiated seromucinous gland tissue, morphologically consistent with salivary gland. Materials and Methods: Biopsy samples were collected for microscopical examination, fixed in 10% neutral buffered formalin, dehydrated, embedded in paraffin wax, sectioned (4 mm) and stained with HE. For comparison, samples of normal salivary gland, lachrymal gland and third eyelid gland were studied. Results: Microscopical examination of the retrobulbar mass revealed seromucinous tubuloalveolar glands with predominance of a mucinous component and presence of intercalated and striated ducts. In some parts of the parenchyma, cystic dilatation of alveoli and ducts was observed. There were no signs of neoplastic changes. Conclusions: A diagnosis of salivary gland heterotopia in the retrobulbar space was made.
CYTOLOGICAL INVESTIGATION OF MAMMARY GLAND TUMOURS IN THE QUEEN AND BITCH M. Militaru, E. Ciobotaru, G. Dinescu, A. Diaconescu, I. Ionascu and D. Cranganu Faculty of Veterinary Medicine, Romania Introduction: The role of cytological examination of mammary gland tumours is controversial. The aim of this study was to establish the degree of correlation between cytological and histopathological examination of such lesions. Materials and Methods: Mammary gland tumours from 39 queens and 88 bitches were investigated. The diagnosis was established by cytological (fine needle aspiration and imprint cytology stained by MGG) and histopathological (Masson’s trichrome stain) methods. Histopathological diagnoses were made according to the WHO classification. Results: The cytological criteria evaluated included morphology, presence of secretory and ductal epithelial cells, mesenchymal cells with and without metaplasia, the presence of inflammatory cells and the background content. The disparity in diagnosis was 7.7% of feline tumours and it was correlated with the presence of cellular disruption with free nuclei, the presence of an inflammatory process and the lack of clinical data. The disparity in diagnosis was 10.2% of canine tumours and it was correlated with the presence of an inflammatory process, stromal metaplasia and the structural complexity of the tumours. Conclusions: Cytological diagnosis of mammary gland tumours is more accurate in the queen than the bitch. In dogs, diagnostic cytology via fine needle aspiration has a limited value, but it is a useful method for determining lymph node metastasis.
P11
P12
POULTRY HEPATOPATHIES AND THEIR CORRELATION WITH FOOD SAFETY M. Militaru, L. Tudor, M.O. Ghimpeteanu, E. Ciobotaru and G. Dinescu Faculty of Veterinary Medicine, Romania Introduction: The nature of liver lesions and their correlation with the isolation of potentially pathogenic microorganisms can provide information about the general health status of a poultry flock and the potential danger to the health of consumers. The aim of this study was to evaluate the association between different types of hepatopathy diagnosed in rejected organs with the presence of some microorganisms. Materials and Methods: Livers (n 5 411) from young abattoirslaughtered poultry were investigated. Cytological (May Gr€ unwald Giemsa stain), histological (Masson’s trichrome and Congo red stains) and microbiological examinations were performed on 22 livers considered representative of different gross lesions. Results: The two major types of lesion were non-specific hepatopathy with diffuse degenerative features associated with vascular reactions and multifocal miliary necrotic hepatitis. Cytologically, the degree of hepatocyte degeneration and the nature of the inflammatory infiltrates were investigated. Histologically, different types of lesions were described including hepatosis, miliary necrotic hepatitis, diffuse interstitial infiltration and amyloidosis. The most common microorganisms isolated were E. coli, Pseudomonas spp. and yeasts. Conclusions: 94.4% of the samples had non-specific hepatopathy and 5.6% had multifocal miliary necrotic hepatitis. Miliary necrotic hepatitis was associated with Pseudomonas spp. (5/7) and non-specific hepatopathy was correlated with isolation of E. coli (8/15), yeasts (8/15) and Enterococcus spp. (7/15).
OBSERVATIONS CONCERNING CADMIUM AS A POLLUTANT ELEMENT IN SOIL AND FORAGES AND ITS INFLUENCE ON ANIMALS IN THE SOUTHERN AREA OF BUCHAREST E. Mitranescu, M. Militaru, L. Tudor, E. Ciobotaru and F. Furnaris Faculty of Veterinary Medicine, Bucharest, Romania Introduction: Cadmium is one of the pollutant elements emitted in the eastern area of Bucharest. It may accumulate in the liver, kidney, heart and brain. Materials and Methods: Soil samples were collected from different depths and distances from the source of pollution, and from forage samples and organs collected from horses, pigs and ruminants. The cadmium concentration was analyzed by atomic absorption spectrophotometry. Animals were subjected to clinical examination followed by gross necropsy examination and microscopical evaluation of tissue sections. Results: Cadmium concentrations exceeded the maximum permitted limits by 2.3 times in soil samples collected from depths under 10 cm and at 200 m distance from the pollution source; by 3.57 times in forage samples; and in organs the limits were exceeded by nine times in horse liver and almost 40 times in horse kidney. High concentrations of cadmium were related to clinical signs (mainly digestive and respiratory) and to major lesions of the kidney and liver (nephritis and hepatosteatosis). Conclusion: The correlation between cadmium concentration in soil, plants and organs and the clinical and pathological findings is consistent with chronic cadmium toxicosis in animals in the eastern area of Bucharest.