Detection and analysis of cancer-related human papilloma virus DNA in bowenoid papulosis by a series of polymerase chain reactions

203 THE EFFECT OF TOPICAL CAPSAICIN ON IRRITANT ALLERGIC CONTACT DERMATITIS IN MICE N YAMASHITA, M NATSUAKI AND S SAGAMI, of dermatology, Hyogo College of MedIcme, l-l cho, Nishinomiya-shi, Hyogo, Japan

AND

Department Mukogawa-

Toplcal capsalcin treatment is known to deplete neuropeptides released from cutaneous sensory nerves We studled the effect of topical capsalcin on primary irritant dermatitis and allergic contact sensitivity in mice. Capsaicin pretreatment caused signlflcant inhlbition of ear swelling as pnmary irritation induced by toplcal application with croton oil. In contrast, capsaicin pretreatment had no Inhibitory effect on allergic contact hypersensitivity reaction to oxazolone These results Indicate that neuropeptides in sensory nerves are Involved In the pathogenesis of primary irritant dermatitis, but not in allergic contact dermatitis.

205

206

REGULATION SENSTIVITY

OF

S.YAXUAKI,

T.SATO,

Departmenr University, After the

LANGERHANS

H.YOKOZEKI,

application

regulatory has

MIGRATION

WRESSION IIFTlPE VII CDLL\CEN \YD Nil-T?-BMZ \NTlGEN l\i~tilIICS ShlI TLMORS

IN CONTACT

I.KATAYAMA

and

'I.'(SAX\ ', h. hl',lEE'.K. TWKI-, '.I. FIJiICE', 1. !SHIBASHI' Uepart.men!of Dermato!og,, \amanashi Yedical l'nlwrsltk. Tsmaho, Yakatxma, 'ramanashl,and 'Department of Dermatologi, "n'!PrSlt:;3f Tokyo, Hongo. BunkJo-ku, Tokyo, Japan

K.NISHIOKA,

of Dermatology, Tokyo Medical and Dental l-S-45, Yushima, Bunkyoku, Tokyo, Japan.

epidermis

(LN)

CELL

of hapten

to

the

mechanism remained

effect

of

hapten

painted

of

Halb/c

chamber

method.

24 hour

after

In

culture

LC

TKCB

skin,

lymph

LC migration

unclear.

Ln‘ cell

to the

regional

study,

mouse

on to

painting

but

LC

(CS),

chemotaxis

CS not

we

obtained

the

lymph

node

examined

the

obtained using from

to control

from

However,

to regional

this

supernatant

migrated

LC migrate

nodes.

from Boyden

mouse

at

medium.

207 DETECTION

AND ANALYSIS

VIRUS

DNA IN BOWENOID

CHAIN

REACTIONS.

SHINICHIRO

YASUMOTO,

Department

of Dermatology

Kyushu

University,

and Department

samples

chain reaction(PCR)s,

DNA was detected when

EG-E7regions

were

of HPVIG.

18, 31.

were posttive in PCR using

I” one of these cases

PCR using GP were applied.

that the transfomatlan-related the cell genome

to detect

wus(HPV)DNA,

prwners (GP) to detect Li region of HPV6.11,16,

16, 31 and 33. Two cases with BP in aged patients

out to be negative

HORI,

Faculty of Medicine,

which are designed

human papllloma

PCR were I) consensus primers(CP)to amplify

HPVIG

THO’ AND YOSHIAKI

of Virology’,

from 4 cases of bowenoid papulosis(BP).Primers used for

33, 52b and 56. and P)general

CP and

PAPILLOMA

612. Japan

relatively broad range of cancer-related applledtothe

HUMAN

BY A SERIES OF POLYMERASE

KOHEI OHTA, YASUSHI

Fukuoka

A series of polymerase

OF CANCER-RELATED PAPULOSIS

Both of them turned

These results suggests

regions of HPV DNA may be integrated

with the deletion

of late regons

into

in some cases wth BP

'Ihewtaneous basement membrane zone (BMZ) Includes a veri IUSE sumber ,I'molecular campownts. 'iehave rewnt):. reported that one of the CDlb monoc!onal antibodies i%Ab), VU-TZ, reacted to the lowrmost lamma Iticidaof the BMZ. In order to further eluctdate the biclogical prnpertles of the WTFBMZ sntigen WE lnvesti:ated the expression of \ll-T?-BMZantigen and type VII collagen (bell identified component 01 :ucharmg fibrlls) in skin tumors such as basal cell epithelioma (BCE), squamous cell carcinoma, Mown's disease. actlnli:keratas~s. scborrheic icratoSLs, soft fibrnma, and mscosis fungoides. #oib agamst r::peill ~:olIageoshowd reduced staining uf the BNZ m LICE.'4oibagainst 'Il-T? !'alledto rt'actto the BMZ in BCE. Both antigens, howb6xr, wre bell !jrererred in the BMZ of ather SK," tumors as did in the normal adwxnt skin. Furthwmare, i;pr VII collagen \1oAudemonstrated tne clear Intral.wou.1 staining in BCE. Thus, abnormal PYP~ESSIO" of YL-T7-YMZ ,:ntigenand Is-peill collagen erasobserred in BCE.