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Detection of HPV in fresh tissue samples, saliva, plasma and oral exfoliated cells extracted from patients with oral leukoplakia
OOOO Volume 114, Number 4 from past 5 years following treatment with phenytoin and phenobarbital drugs for tonic-clonic seizures. Study design: Patient had no other systemic disease and was partially edentulous with no history of prosthodontic treatment. Intraoral examination revealed abundant calculus, depressible mobility of teeth along with lobulated and fibrotic consistency gingival overgrowth in dentate and edentulous regions of upper and lower arches. For treatment, full mouth extraction and surgical excision of overgrowths was performed along with drug replacement with sodium valproate. Results: Histologically, the lesion showed fibro-epithelial hyperplasia. Clinical results after six months demonstrated almost complete resolution of gingival overgrowth. Conclusions: The findings of present case suggest that gingival overgrowth can occur even in partially edentulous ridges (not exposed to denture wear) that could be due to persistence of gingival overgrowth, which may not resolve completely following tooth extraction or occurs because of incorporation of specific subpopulation of gingival fibroblasts in alveolar ridge mucosa.
PREVENTION AND EARLY DETECTION OF ORAL CANCER IN BRAZIL-PARANA STATE BETWEEN 2008-2011: PERIOD: V Sassi LM1,2, Dissenha JL1, Guebur MI1, Zanferrari FL1, Nascimento L3, Oliveira BV4, Orlandi D5 - 1HOSPITAL ERASTO GAERTNER DE CURITIBA - SERVIÇO DE CIRURGIA BUCO-MAXILO-FACIAL/HOSPITAL ERASTO GAERTNER, 2HOSPITAL ERASTO GAERTNER DE CURITIBA - CHEFE DO SERVIÇO DE CIRURGIA BUCOMAXILO-FACIAL, 3HOSPITAL ERASTO GAERTNER/ UFPR - SERVIÇO DE CIRURGIA BUCO-MAXILOFACIAL/HOSPITAL ERASTO GAERTNER, 4HOSPITAL ERASTO GAERTNER DE CURITIBA - CHEFE DO SERVIÇO DE CIRURGIA CABEÇA E PESCOÇO, 5HOSPITAL ERASTO GAERTNER DE CURITIBA - ESTATISTICA/ REGISTRO HOSPITALAR Preventing oral cancer in the period 1989 to 2011, broken out of 5 in 5 years. Objectives: Prevention of cancer in the community. Method 21,018 patients in 98 Municipalities in Paraná examined between 1989 to 2011. This period from 2008 to 2011. Results: We studied 4.240 individuals, 61,3% female 82,5% Caucasian, family income ⬍2 minimum (Under-paid (U$ 300.00) wages was reported by 60,2%; Poorly-educated (primary school or below, 465(59,8%), illiterate, 102(13,1%), High School 143(16,4%), University 48(19,8%). Were found and sent to municipal ambulatories 778(18,3%) patients with lesions aged between 31 and 70 were 76%. Of these, 206(26,5%)were inflammatory, trauma 336(43,2%), and 52(6,7%), leukoplakia, other 76(9,8. Dentist Frequency With pain 209(26,9%), Don’t go 181(23,3%), Without teeth 149(19,2%), 1x/year 139(17,9,1%), 1x /2year 67(8,6%), 35,9% Regular oral hygiene, alcoholism 17.0%; smoking 24.5%; 42,8% consumption of matei; Had never undergone preventive exams 54(6,9%), Make 78(10%), Didn’t 615(79,0%). Conclusion: New health care policies should be made
POTENTIAL ROLE OF SET PROTEIN IN ORAL SQUAMOUS CELL CARCINOMA TUMORIGENESIS Sobral LM1, Sousa LO1, Coletta RD2, Gutkind S3, Leopoldino AM1 1 FAC. DE CIÊNCIAS FARMACÊUTICAS DE RIBEIRÃO
ORAL ABSTRACT Abstracts e121 PRETO- USP - DEPARTAMENTO DE ANÁLISES CLÍNICAS, TOXICOLÓGICAS E BROMATOLÓGICAS, 2FAC. DE ODONTOLOGIA DE PIRACICABA-UNICAMP - DEPARTAMENTO DE DIAGNÓSTICO ORAL, PATOLOGIA ORAL, 3 NATIONAL INSTITUTE OF DENTAL AND CRANIOFACIAL RESEARCH, NATIONAL INSTITUTES OF HEALTH ORAL AND PHARYNGEAL CANCER BRANCH Objectives: To assess the potential role of SET protein in oral squamous cell carcinoma (OSCC) tumorigenesis, in vitro and in vivo. Study design: SET knockdown was stably obtained in HN12 cells. Cell migration and invasion were analyzed through Boyden chamber assay and proliferation through BrdU. In vivo, Balb/C nude mice (n⫽10) were injected in the flank with shControl or shSET HN12 cells to obtain xenograft tumors. These tumors were characterized histopathologically and analyzed by immunohistochemistry for SET, E-cadherin and alpha-SMA. Results: SET knockdown increased HN12 cell migration and invasion but did not affect cell proliferation in vitro. Xenograft tumors derived from shSET HN12 cells showed fast growing with large amount of necrosis and were histopathologically more aggressive than ones derived from shControl. Conclusion: SET levels have implication in tumor differentiation in OSCC. Financial support: FAPESP and CNPq.
METHYLATION PROFILE OF CCNA1, P16, HIC1, TIMP3, MGMT AND DCC GENES IN HEAD AND NECK CANCER Arantes LMRB1, de Carvalho AC2, Tajara EH1, Carvalho AL3, Goloni-Bertollo EM1, GENCAPO - 1SÃO JOSÉ DO RIO PRETO MEDICAL SCHOOL (FAMERP) DEPARTMENT OF MOLECULAR BIOLOGY, 2FEDERAL UNIVERSITY OF SÃO PAULO (UNIFESP) - LABORATORY FOR MOLECULAR CANCER BIOLOGY, DEPARTMENT OF BIOCHEMISTRY, 3BARRETOS CANCER HOSPITALPIO XII - CENTER FOR RESEARCH IN MOLECULAR ONCOLOGY Background: Head and neck carcinomas are among the most frequent tumor types worldwide with around 200,000 new cases diagnosed per year. The most common variant is squamous cell carcinoma (HNSCC), representing 90% of these malignancies. Activation of proto-oncogenes and inactivation of tumor suppressor genes are the major genetic alterations involved in carcinogenesis. Several tumor suppressor genes and genes regulating growth and development are silenced in cancer by aberrant gene methylation. Objective: To determinate the hypermethylation profile of CCNA1, P16, HIC1, TIMP3, MGMT and DCC. Study design: Nine DNA samples from HNSCC tumors were evaluated using Q-MSP. Results: HIC1 showed promoter hypermethylation in 78%, MGMT in 75%, TIMP3 in 63%, CCNA1 in 44%, DCC in 37% and P16 in 11% of the tumors. Conclusion: These results show that the methylation of tumor suppressor genes is a common event in HNSCC. More samples should be evaluated to suggest an association with clinical features.
DETECTION OF HPV IN FRESH TISSUE SAMPLES, SALIVA, PLASMA AND ORAL EXFOLIATED CELLS EX-
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Abstracts
TRACTED FROM PATIENTS WITH ORAL LEUKOPLAKIA Ferreira LL1, Soares GR1, Veronese LA2, Nunes CM3, Bernabé DG1, Biasoli ER1, Miyahara GI1 - 1FACULDADE DE ODONTOLOGIA DE ARAÇATUBA- UNESP - CENTRO DE ONCOLOGIA BUCAL, 2VERONESE PATOLOGIA E CITOLOGIA, 3FACULDADE DE MEDICINA VETERINÁRIA DE ARAÇATUBA- UNESP - DEPARTAMENTO DE APOIO, PRODUÇÃO E SAÚDE ANIMAL Background: The oral leukoplakia is considered as a premalignant lesion for the development of the oral squamous cell carcinoma, and several risk factors can be related to this carcinogenesis, including the human papillomavirus (HPV). Objectives: The aims of this study was to detect the presence of HPV DNA in fresh tissue samples, plasma and oral exfoliated cells extracted from patients with oral leukoplakia. Study design: These biological materials sources were analyzed through the technique of nPCR and make a comparison among them from 8 patients with oral leukoplakia. It was performed the amplification of the human -globin gene in all samples to confirm the presence and integrity of DNA. Results: Nested PCR assays revealed the presence of HPV in 37.5% of fresh tissue, 50.0% of plasma, 62.5% of oral exfoliated cells extracted from patients. Conclusion: HPV could potentially be an etiologic co-factor in the pathogenesis of oral leukoplakia.
PROTEOMIC PROFILE IN SERUM OF HAMSTER DURING DMBA-INDUCED ORAL SQUAMOUS CELL CARCINOGENESIS LM Lin1,2,3,4,5,6,7 - 1LI-MIN LIN - KAOHSIUNG MEDICAL UNIVERSITY HOSPITAL, DEPARTMENTS OF ORAL PATHOLOGY AND ORAL AND MAXILLOFACIAL IMAGING CENTER, 2YAN-HSIUNG WANG SCHOOL OF DENTISTRY, 3WEN-CHEN WANG - DEPARTMENTS OF ORAL PATHOLOGY AND ORAL AND MAXILLOFACIAL IMAGING CENTER, 4ANDY TENG - SCHOOL OF DENTISTRY, 5SHIH-HSIEN CHEN - DEPARTMENTS OF RADIOLOGY, 6CHING-YI CHEN - DEPARTMENTS OF ORAL PATHOLOGY AND ORAL AND MAXILLOFACIAL IMAGING CENTER, 7YUK-KWAN CHEN - DEPARTMENTS OF ORAL PATHOLOGY AND ORAL AND MAXILLOFACIAL IMAGING CENTER Background: In our previous report, we found that some proteomic biomarkers like transthyretin & paraoxonase 1 were strongly decrease after tumor inoculation in the serum during the development of Vx2-induced rabbit OSCC. Objectives: The purpose of study is to use a proteomic analysis in serum of hamsters during DMBA-induced OSCC carcinogenesis in order to observe the difference between early and late stage of tumors, ultimately to identify novel-invasive serum biomarkers of OSCC. Study design: Twelve adult Syrian golden hamsters were divided into one control and three experimental groups, each group containing three animals. In the experimental groups, 0.5% DMBA in heavy mineral oil was painted at hamster right cheek pouches for 4, 9 and 13 weeks period respectively. All serum samples were collected and stored at ⫺80 °C before 2D gel electrophoresis analysis. Fifteen protein spots were visualized by silver staining and identified by liquid chromatography/tandem mass spectrometry, such as C-reactive protein, complement factor B, complement C3, immunoglobulin kappa-chain, liver carboxylesterase, and haptoglobin.
October 2012 Results and conclusion: The protein level of C-reactive protein and haptoglobin were decreased at 4 weeks and then increased at 9 and 13 weeks treatment. Other proteins were decreased with time. The relationship between these biomarkers and OSCC development remains to be further tested in cancer patients.
ODONTOLOGIC URGENCY AFTER TOOTH EXTRACTION IN A PATIENT WITH PLATELET HYPOAGGREGATION: CASE REPORT Alexandre CN, Silveira EO, Assunção MCS, Queiroz PRK, Silva JF, Teixeira L, Rego LCB The platelets disorders causes signals and symptoms characteristic of hemostasis defects and it can cause abnormal bleeding during the surgical procedure, for example tooth extraction. Patient, male, 14 years old, was forwarded to a dental assessment in a reference Center to Treatment of Hematological Diseases, with alveolar bleeding intense, immediately after being submitted of a extraction lower molar. It was done local hemostatic maneuvers as urgency procedure by simple suture stabilizing clot associated to tranexamic acid in paste form (1 pill 250mg crushed in solution 250mg/5mL) which was placed under the surgical wound. These measurements were sufficient to provide the control of bleeding site. Blood tests were required to analyze a possibility of coagulations disorders. The hematologic exams showed: normal hemogram and normal platelet count, moreover a normality of factories V, VIII, IX, X, XII, Von Willebrand, however the aggregation test (plates aggregate with adrenaline) showed platelet hypo aggregation. REFERÊNCIAS: Luz Ld, Sankarankutty A, Passos E, Rizoli S, Fraga GP, Nascimento Jr B. Tranexamic acid for traumatic hemorrhage. Rev. Col. Bras. Cir. 2012; 39(1): 77-80. Adeyemo TA, Adeyemo WL, Adediran A, Akinbami AJ, Akanmu AS. Orofacial manifestations of hematological disorders: anemia and hemostatic disorders. Indian J Dent Res. 2011; 22(3):454-61. Lambert MP. What to do when you suspect an inherited platelet disorder. Hematology Am Soc Hematol Educ Program. 2011; 2011:377-83. Brasil. Manual de Tratamento das Coagulopatias Hereditárias. Ministério da Saúde, Secretaria de Atenção à Saúde, Departamento de Atenção Especializada. Ministério da Saúde. 2006 Brasil. Manual de Atendimento Odontológico a Pacientes com Coagulopatias Hereditárias. Ministério da Saúde, Secretaria de Atenção à Saúde, Departamento de Atenção Especializada. Ministério da Saúde. 2008
ANALYSIS OF THE EFFECTS OF ORLISTAT ON THE IMMUNE RESPONSE AGAINST EXPERIMENTAL MELANOMAS Almeida LY1, Mariano FS1, Coletta RD1, Agostini M2, Bastos DC1, Seguin F1, Graner E1 - 1PIRACICABA DENTAL SCHOOL, STATE UNIVERSITY OF CAMPINAS (UNICAMP) - ORAL DIAGNOSIS DEPARTMENT, 2SCHOOL OF DENTISTRY, FEDERAL UNIVERSITY OF RIO DE JANEIRO (UFRJ) - DEPARTMENT OF PATHOLOGY AND ORAL DIAGNOSIS Background: Orlistat (Xenical®) has anti-neoplastic properties by irreversibly inhibiting fatty acid synthase (FASN). Objective: Our work aimed to study the effect of Orlistat on the percentage and activation of CD8⫹ T lymphocytes
PREVENTION AND EARLY DETECTION OF ORAL CANCER IN BRAZIL-PARANA STATE BETWEEN 2008-2011: PERIOD: V Sassi LM1,2, Dissenha JL1, Guebur MI1, Zanferrari FL1, Nascimento L3, Oliveira BV4, Orlandi D5 - 1HOSPITAL ERASTO GAERTNER DE CURITIBA - SERVIÇO DE CIRURGIA BUCO-MAXILO-FACIAL/HOSPITAL ERASTO GAERTNER, 2HOSPITAL ERASTO GAERTNER DE CURITIBA - CHEFE DO SERVIÇO DE CIRURGIA BUCOMAXILO-FACIAL, 3HOSPITAL ERASTO GAERTNER/ UFPR - SERVIÇO DE CIRURGIA BUCO-MAXILOFACIAL/HOSPITAL ERASTO GAERTNER, 4HOSPITAL ERASTO GAERTNER DE CURITIBA - CHEFE DO SERVIÇO DE CIRURGIA CABEÇA E PESCOÇO, 5HOSPITAL ERASTO GAERTNER DE CURITIBA - ESTATISTICA/ REGISTRO HOSPITALAR Preventing oral cancer in the period 1989 to 2011, broken out of 5 in 5 years. Objectives: Prevention of cancer in the community. Method 21,018 patients in 98 Municipalities in Paraná examined between 1989 to 2011. This period from 2008 to 2011. Results: We studied 4.240 individuals, 61,3% female 82,5% Caucasian, family income ⬍2 minimum (Under-paid (U$ 300.00) wages was reported by 60,2%; Poorly-educated (primary school or below, 465(59,8%), illiterate, 102(13,1%), High School 143(16,4%), University 48(19,8%). Were found and sent to municipal ambulatories 778(18,3%) patients with lesions aged between 31 and 70 were 76%. Of these, 206(26,5%)were inflammatory, trauma 336(43,2%), and 52(6,7%), leukoplakia, other 76(9,8. Dentist Frequency With pain 209(26,9%), Don’t go 181(23,3%), Without teeth 149(19,2%), 1x/year 139(17,9,1%), 1x /2year 67(8,6%), 35,9% Regular oral hygiene, alcoholism 17.0%; smoking 24.5%; 42,8% consumption of matei; Had never undergone preventive exams 54(6,9%), Make 78(10%), Didn’t 615(79,0%). Conclusion: New health care policies should be made
POTENTIAL ROLE OF SET PROTEIN IN ORAL SQUAMOUS CELL CARCINOMA TUMORIGENESIS Sobral LM1, Sousa LO1, Coletta RD2, Gutkind S3, Leopoldino AM1 1 FAC. DE CIÊNCIAS FARMACÊUTICAS DE RIBEIRÃO
ORAL ABSTRACT Abstracts e121 PRETO- USP - DEPARTAMENTO DE ANÁLISES CLÍNICAS, TOXICOLÓGICAS E BROMATOLÓGICAS, 2FAC. DE ODONTOLOGIA DE PIRACICABA-UNICAMP - DEPARTAMENTO DE DIAGNÓSTICO ORAL, PATOLOGIA ORAL, 3 NATIONAL INSTITUTE OF DENTAL AND CRANIOFACIAL RESEARCH, NATIONAL INSTITUTES OF HEALTH ORAL AND PHARYNGEAL CANCER BRANCH Objectives: To assess the potential role of SET protein in oral squamous cell carcinoma (OSCC) tumorigenesis, in vitro and in vivo. Study design: SET knockdown was stably obtained in HN12 cells. Cell migration and invasion were analyzed through Boyden chamber assay and proliferation through BrdU. In vivo, Balb/C nude mice (n⫽10) were injected in the flank with shControl or shSET HN12 cells to obtain xenograft tumors. These tumors were characterized histopathologically and analyzed by immunohistochemistry for SET, E-cadherin and alpha-SMA. Results: SET knockdown increased HN12 cell migration and invasion but did not affect cell proliferation in vitro. Xenograft tumors derived from shSET HN12 cells showed fast growing with large amount of necrosis and were histopathologically more aggressive than ones derived from shControl. Conclusion: SET levels have implication in tumor differentiation in OSCC. Financial support: FAPESP and CNPq.
METHYLATION PROFILE OF CCNA1, P16, HIC1, TIMP3, MGMT AND DCC GENES IN HEAD AND NECK CANCER Arantes LMRB1, de Carvalho AC2, Tajara EH1, Carvalho AL3, Goloni-Bertollo EM1, GENCAPO - 1SÃO JOSÉ DO RIO PRETO MEDICAL SCHOOL (FAMERP) DEPARTMENT OF MOLECULAR BIOLOGY, 2FEDERAL UNIVERSITY OF SÃO PAULO (UNIFESP) - LABORATORY FOR MOLECULAR CANCER BIOLOGY, DEPARTMENT OF BIOCHEMISTRY, 3BARRETOS CANCER HOSPITALPIO XII - CENTER FOR RESEARCH IN MOLECULAR ONCOLOGY Background: Head and neck carcinomas are among the most frequent tumor types worldwide with around 200,000 new cases diagnosed per year. The most common variant is squamous cell carcinoma (HNSCC), representing 90% of these malignancies. Activation of proto-oncogenes and inactivation of tumor suppressor genes are the major genetic alterations involved in carcinogenesis. Several tumor suppressor genes and genes regulating growth and development are silenced in cancer by aberrant gene methylation. Objective: To determinate the hypermethylation profile of CCNA1, P16, HIC1, TIMP3, MGMT and DCC. Study design: Nine DNA samples from HNSCC tumors were evaluated using Q-MSP. Results: HIC1 showed promoter hypermethylation in 78%, MGMT in 75%, TIMP3 in 63%, CCNA1 in 44%, DCC in 37% and P16 in 11% of the tumors. Conclusion: These results show that the methylation of tumor suppressor genes is a common event in HNSCC. More samples should be evaluated to suggest an association with clinical features.
DETECTION OF HPV IN FRESH TISSUE SAMPLES, SALIVA, PLASMA AND ORAL EXFOLIATED CELLS EX-
OOOO
e122
Abstracts
TRACTED FROM PATIENTS WITH ORAL LEUKOPLAKIA Ferreira LL1, Soares GR1, Veronese LA2, Nunes CM3, Bernabé DG1, Biasoli ER1, Miyahara GI1 - 1FACULDADE DE ODONTOLOGIA DE ARAÇATUBA- UNESP - CENTRO DE ONCOLOGIA BUCAL, 2VERONESE PATOLOGIA E CITOLOGIA, 3FACULDADE DE MEDICINA VETERINÁRIA DE ARAÇATUBA- UNESP - DEPARTAMENTO DE APOIO, PRODUÇÃO E SAÚDE ANIMAL Background: The oral leukoplakia is considered as a premalignant lesion for the development of the oral squamous cell carcinoma, and several risk factors can be related to this carcinogenesis, including the human papillomavirus (HPV). Objectives: The aims of this study was to detect the presence of HPV DNA in fresh tissue samples, plasma and oral exfoliated cells extracted from patients with oral leukoplakia. Study design: These biological materials sources were analyzed through the technique of nPCR and make a comparison among them from 8 patients with oral leukoplakia. It was performed the amplification of the human -globin gene in all samples to confirm the presence and integrity of DNA. Results: Nested PCR assays revealed the presence of HPV in 37.5% of fresh tissue, 50.0% of plasma, 62.5% of oral exfoliated cells extracted from patients. Conclusion: HPV could potentially be an etiologic co-factor in the pathogenesis of oral leukoplakia.
PROTEOMIC PROFILE IN SERUM OF HAMSTER DURING DMBA-INDUCED ORAL SQUAMOUS CELL CARCINOGENESIS LM Lin1,2,3,4,5,6,7 - 1LI-MIN LIN - KAOHSIUNG MEDICAL UNIVERSITY HOSPITAL, DEPARTMENTS OF ORAL PATHOLOGY AND ORAL AND MAXILLOFACIAL IMAGING CENTER, 2YAN-HSIUNG WANG SCHOOL OF DENTISTRY, 3WEN-CHEN WANG - DEPARTMENTS OF ORAL PATHOLOGY AND ORAL AND MAXILLOFACIAL IMAGING CENTER, 4ANDY TENG - SCHOOL OF DENTISTRY, 5SHIH-HSIEN CHEN - DEPARTMENTS OF RADIOLOGY, 6CHING-YI CHEN - DEPARTMENTS OF ORAL PATHOLOGY AND ORAL AND MAXILLOFACIAL IMAGING CENTER, 7YUK-KWAN CHEN - DEPARTMENTS OF ORAL PATHOLOGY AND ORAL AND MAXILLOFACIAL IMAGING CENTER Background: In our previous report, we found that some proteomic biomarkers like transthyretin & paraoxonase 1 were strongly decrease after tumor inoculation in the serum during the development of Vx2-induced rabbit OSCC. Objectives: The purpose of study is to use a proteomic analysis in serum of hamsters during DMBA-induced OSCC carcinogenesis in order to observe the difference between early and late stage of tumors, ultimately to identify novel-invasive serum biomarkers of OSCC. Study design: Twelve adult Syrian golden hamsters were divided into one control and three experimental groups, each group containing three animals. In the experimental groups, 0.5% DMBA in heavy mineral oil was painted at hamster right cheek pouches for 4, 9 and 13 weeks period respectively. All serum samples were collected and stored at ⫺80 °C before 2D gel electrophoresis analysis. Fifteen protein spots were visualized by silver staining and identified by liquid chromatography/tandem mass spectrometry, such as C-reactive protein, complement factor B, complement C3, immunoglobulin kappa-chain, liver carboxylesterase, and haptoglobin.
October 2012 Results and conclusion: The protein level of C-reactive protein and haptoglobin were decreased at 4 weeks and then increased at 9 and 13 weeks treatment. Other proteins were decreased with time. The relationship between these biomarkers and OSCC development remains to be further tested in cancer patients.
ODONTOLOGIC URGENCY AFTER TOOTH EXTRACTION IN A PATIENT WITH PLATELET HYPOAGGREGATION: CASE REPORT Alexandre CN, Silveira EO, Assunção MCS, Queiroz PRK, Silva JF, Teixeira L, Rego LCB The platelets disorders causes signals and symptoms characteristic of hemostasis defects and it can cause abnormal bleeding during the surgical procedure, for example tooth extraction. Patient, male, 14 years old, was forwarded to a dental assessment in a reference Center to Treatment of Hematological Diseases, with alveolar bleeding intense, immediately after being submitted of a extraction lower molar. It was done local hemostatic maneuvers as urgency procedure by simple suture stabilizing clot associated to tranexamic acid in paste form (1 pill 250mg crushed in solution 250mg/5mL) which was placed under the surgical wound. These measurements were sufficient to provide the control of bleeding site. Blood tests were required to analyze a possibility of coagulations disorders. The hematologic exams showed: normal hemogram and normal platelet count, moreover a normality of factories V, VIII, IX, X, XII, Von Willebrand, however the aggregation test (plates aggregate with adrenaline) showed platelet hypo aggregation. REFERÊNCIAS: Luz Ld, Sankarankutty A, Passos E, Rizoli S, Fraga GP, Nascimento Jr B. Tranexamic acid for traumatic hemorrhage. Rev. Col. Bras. Cir. 2012; 39(1): 77-80. Adeyemo TA, Adeyemo WL, Adediran A, Akinbami AJ, Akanmu AS. Orofacial manifestations of hematological disorders: anemia and hemostatic disorders. Indian J Dent Res. 2011; 22(3):454-61. Lambert MP. What to do when you suspect an inherited platelet disorder. Hematology Am Soc Hematol Educ Program. 2011; 2011:377-83. Brasil. Manual de Tratamento das Coagulopatias Hereditárias. Ministério da Saúde, Secretaria de Atenção à Saúde, Departamento de Atenção Especializada. Ministério da Saúde. 2006 Brasil. Manual de Atendimento Odontológico a Pacientes com Coagulopatias Hereditárias. Ministério da Saúde, Secretaria de Atenção à Saúde, Departamento de Atenção Especializada. Ministério da Saúde. 2008
ANALYSIS OF THE EFFECTS OF ORLISTAT ON THE IMMUNE RESPONSE AGAINST EXPERIMENTAL MELANOMAS Almeida LY1, Mariano FS1, Coletta RD1, Agostini M2, Bastos DC1, Seguin F1, Graner E1 - 1PIRACICABA DENTAL SCHOOL, STATE UNIVERSITY OF CAMPINAS (UNICAMP) - ORAL DIAGNOSIS DEPARTMENT, 2SCHOOL OF DENTISTRY, FEDERAL UNIVERSITY OF RIO DE JANEIRO (UFRJ) - DEPARTMENT OF PATHOLOGY AND ORAL DIAGNOSIS Background: Orlistat (Xenical®) has anti-neoplastic properties by irreversibly inhibiting fatty acid synthase (FASN). Objective: Our work aimed to study the effect of Orlistat on the percentage and activation of CD8⫹ T lymphocytes