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PROTEIN EXPRESSION IN SALIVA SAMPLES FROM PATIENTS WITH ORAL MALIGNANT LESIONS
ABSTRACTS
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Results: Of these patients, 91% were male and 8.5% female, 94.6% were white, and 29.8% were rural workers with mean age of 49.3 years. Eighty-five percent of the patients were smokers and 56.3% were alcohol drinkers. The painful complaint was present in 82% of the patients. Mulberry-like ulcers was the most frequent lesion 87%, healing in 8 weeks in average. The main clinical signs were swelling 88%, bleeding 37%, and erythema 14%. The time until to patient came to the clinic was 14 weeks on average. The most affected sites were tongue 47%, buccal mucosa and palate 41%, and lips 35%. Conclusions: PCM occur predominantly in white men, and the main clinical aspect was painful mulberry-like ulcer on the tongue. The recognition of oral lesions is important because sometimes they are the first manifestation of PCM, frequently preceding even pulmonary lesions.
MIR-24 EXPRESSION IN SALIVA SAMPLES FROM PATIENTS WITH ORAL MALIGNANT LESIONS. CLAUDIA REBECCA COSTA CAVALCANTE SILVA, MIRELA GODOI NUNES DE OLIVEIRA, LAURA FREIRE DE CARVALHO, JESSICA RAYANE OLIVEIRA MELO, VANESSA DE CARLA BATISTA DOS SANTOS, SONIA MARIA SOARES FERREIRA and, CARLOS ARTHUR CARDOSO ALMEIDA Objective: To evaluate the expression of miR-24 in saliva collected from patients diagnosed with oral cancer. Study Design: Plasma and saliva samples were spontaneously collected from 03 healthy donors (controls) and 8 patients with oral cancer. MicroRNA extraction was done using trizol reagent and quantified by spectrophotometry. cDNA was synthesized using the GoScriptT Reverse Transcription kit (PROMEGA). Then, miR-24 expression was analyzed using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay. b-globin was used as reference gene. Results: Seven patients were diagnosed with oral squamous cell carcinoma and 1 with myoepithelial carcinoma. Patients presented single and ulcerated lesions and absence of metastasis. Most of them were men (62.5%) whose average age was 64.5 years. In plasma samples, there were no difference in expression levels between controls and patients samples; however, in saliva, miR-24 showed overexpression (4.0-fold; P < .05) in patients with oral cancer in comparison with healthy controls. Conclusions: (1) These results suggest that the miR-24 might be a possible candidate as molecular biomarker to diagnose oral cancer through saliva; however, further studies need to be done in order to validate these results and (2) Saliva is an important source of biomolecules suitable to be validated as molecular markers to diagnose oral diseases.
PROTEIN EXPRESSION IN SALIVA SAMPLES FROM PATIENTS WITH ORAL MALIGNANT LESIONS. CLAUDIA REBECCA COSTA CAVALCANTE SILVA, MIRELA GODOI NUNES DE OLIVEIRA, IVAN JOSE CORREIA NETO, VANESSA DE CARLA BATISTA DOS SANTOS, SONIA MARIA SOARES FERREIRA, TICIANO GOMES DO NASCIMENTO and, CARLOS ARTHUR CARDOSO ALMEIDA Objective: To identify proteins expressed in saliva from patients diagnosed with oral cancer (OC) using high-
OOOO January 2020 performance liquid chromatography with ultraviolet diode array detection (HPLC-UV-DAD-Fluor). Study Design: Saliva samples were collected from 3 healthy donors (controls) and 7 patients with OC. After collecting the samples, were stored at -80˚C for posterior analysis. Samples from both groups were prepared and injected into the high performance liquid chromatography coulpled to UV-DAD and fluorescence system, which had acetonitrile and trifluoroacetic acid as mobile phase solvents in gradient mode. Results: Seven patients had oral squamous cell carcinoma and 1 had myoepithelial carcinoma. The patients presented single and ulcerated lesions and absence of metastasis. Most of them were men (62.5%) with average age of 64.5 years old. Corresponding peaks were detected in control and patients’ samples into the time interval 0 to 2.5 minutes of the chromatogram. Interval 2.5 to 5 minutes presented peaks only in patients’ samples and 5 to 7.5 minutes displayed divergent and higher intensity peaks in patients’ saliva samples. Conclusions: (1) HPLC-UV-DAD-Fluor can be a useful method to screen protein expression in saliva; (2) The HPLCUV-DAD-Fluor analysis showed different protein expression in saliva from controls and from patients suggesting that these proteins may possibly be important candidates for biomarkers to diagnose early OC through saliva.
RECOUNT AND DIVERSITY OF YEAST SPECIES OF GENUS CANDIDA AND ITS ASSOCIATION WITH SALIVARY PH IN PATIENTS WITH TYPE 2 DIABETES. JUAN AITKEN, ANDRESSA WENNESHEIMER, JAIME GONZALEZ and, SANDRA TARQUINIO Objective: To determine number and species of Candida present in saliva according to the glycated hemoglobin (HbA1c) values and salivary pH in patients with type 2 diabetes mellitus (DM2). Material and Methods: Samples of unstimulated saliva were collected from 52 patients diagnosed with DM2. Glycated hemoglobin was obtained from medical records. The pH was measured and the saliva was grown in the sabouraud agar. The values of the colony-forming units (CFU/mL) were calculated and the species of Candida were presumptively identified using Chromagar Candida plaques. The identification was confirmed by polymerase chain reaction (PCR). Results were expressed as median and interquartile range using Kruskal-Wallis test. Results: Amount of CFUs was higher in decompensated patients (P = .025). The most isolated species in the sample was C. albicans (66.1%) followed by C. glabrata (20.7%). Predominant species in compensated patients was C. tropicalis, while in decompensated patients, were C. albicans and C. krusei. There was an inverse association between the value of HbA1c and salivary pH. At lower values of salivary pH, more diversity and quantity of Candida yeasts was observed. Conclusions: Changes in oral microbiota are related to changes in the salivary pH associated with metabolic control of type 2 diabetes.
ADENOMATOID ODONTOGENIC TUMOR. A CLINICAL-PATHOLOGIC STUDY OF 27 CASES. DIANA GABRIELA MORI ALIAGA, ALONDRA HORMAZABAL HEVIA, IRIS ESPINOZA GOMEZ SANTANDER, ANA ORTEGA PINTO, FERNAN
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Results: Of these patients, 91% were male and 8.5% female, 94.6% were white, and 29.8% were rural workers with mean age of 49.3 years. Eighty-five percent of the patients were smokers and 56.3% were alcohol drinkers. The painful complaint was present in 82% of the patients. Mulberry-like ulcers was the most frequent lesion 87%, healing in 8 weeks in average. The main clinical signs were swelling 88%, bleeding 37%, and erythema 14%. The time until to patient came to the clinic was 14 weeks on average. The most affected sites were tongue 47%, buccal mucosa and palate 41%, and lips 35%. Conclusions: PCM occur predominantly in white men, and the main clinical aspect was painful mulberry-like ulcer on the tongue. The recognition of oral lesions is important because sometimes they are the first manifestation of PCM, frequently preceding even pulmonary lesions.
MIR-24 EXPRESSION IN SALIVA SAMPLES FROM PATIENTS WITH ORAL MALIGNANT LESIONS. CLAUDIA REBECCA COSTA CAVALCANTE SILVA, MIRELA GODOI NUNES DE OLIVEIRA, LAURA FREIRE DE CARVALHO, JESSICA RAYANE OLIVEIRA MELO, VANESSA DE CARLA BATISTA DOS SANTOS, SONIA MARIA SOARES FERREIRA and, CARLOS ARTHUR CARDOSO ALMEIDA Objective: To evaluate the expression of miR-24 in saliva collected from patients diagnosed with oral cancer. Study Design: Plasma and saliva samples were spontaneously collected from 03 healthy donors (controls) and 8 patients with oral cancer. MicroRNA extraction was done using trizol reagent and quantified by spectrophotometry. cDNA was synthesized using the GoScriptT Reverse Transcription kit (PROMEGA). Then, miR-24 expression was analyzed using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay. b-globin was used as reference gene. Results: Seven patients were diagnosed with oral squamous cell carcinoma and 1 with myoepithelial carcinoma. Patients presented single and ulcerated lesions and absence of metastasis. Most of them were men (62.5%) whose average age was 64.5 years. In plasma samples, there were no difference in expression levels between controls and patients samples; however, in saliva, miR-24 showed overexpression (4.0-fold; P < .05) in patients with oral cancer in comparison with healthy controls. Conclusions: (1) These results suggest that the miR-24 might be a possible candidate as molecular biomarker to diagnose oral cancer through saliva; however, further studies need to be done in order to validate these results and (2) Saliva is an important source of biomolecules suitable to be validated as molecular markers to diagnose oral diseases.
PROTEIN EXPRESSION IN SALIVA SAMPLES FROM PATIENTS WITH ORAL MALIGNANT LESIONS. CLAUDIA REBECCA COSTA CAVALCANTE SILVA, MIRELA GODOI NUNES DE OLIVEIRA, IVAN JOSE CORREIA NETO, VANESSA DE CARLA BATISTA DOS SANTOS, SONIA MARIA SOARES FERREIRA, TICIANO GOMES DO NASCIMENTO and, CARLOS ARTHUR CARDOSO ALMEIDA Objective: To identify proteins expressed in saliva from patients diagnosed with oral cancer (OC) using high-
OOOO January 2020 performance liquid chromatography with ultraviolet diode array detection (HPLC-UV-DAD-Fluor). Study Design: Saliva samples were collected from 3 healthy donors (controls) and 7 patients with OC. After collecting the samples, were stored at -80˚C for posterior analysis. Samples from both groups were prepared and injected into the high performance liquid chromatography coulpled to UV-DAD and fluorescence system, which had acetonitrile and trifluoroacetic acid as mobile phase solvents in gradient mode. Results: Seven patients had oral squamous cell carcinoma and 1 had myoepithelial carcinoma. The patients presented single and ulcerated lesions and absence of metastasis. Most of them were men (62.5%) with average age of 64.5 years old. Corresponding peaks were detected in control and patients’ samples into the time interval 0 to 2.5 minutes of the chromatogram. Interval 2.5 to 5 minutes presented peaks only in patients’ samples and 5 to 7.5 minutes displayed divergent and higher intensity peaks in patients’ saliva samples. Conclusions: (1) HPLC-UV-DAD-Fluor can be a useful method to screen protein expression in saliva; (2) The HPLCUV-DAD-Fluor analysis showed different protein expression in saliva from controls and from patients suggesting that these proteins may possibly be important candidates for biomarkers to diagnose early OC through saliva.
RECOUNT AND DIVERSITY OF YEAST SPECIES OF GENUS CANDIDA AND ITS ASSOCIATION WITH SALIVARY PH IN PATIENTS WITH TYPE 2 DIABETES. JUAN AITKEN, ANDRESSA WENNESHEIMER, JAIME GONZALEZ and, SANDRA TARQUINIO Objective: To determine number and species of Candida present in saliva according to the glycated hemoglobin (HbA1c) values and salivary pH in patients with type 2 diabetes mellitus (DM2). Material and Methods: Samples of unstimulated saliva were collected from 52 patients diagnosed with DM2. Glycated hemoglobin was obtained from medical records. The pH was measured and the saliva was grown in the sabouraud agar. The values of the colony-forming units (CFU/mL) were calculated and the species of Candida were presumptively identified using Chromagar Candida plaques. The identification was confirmed by polymerase chain reaction (PCR). Results were expressed as median and interquartile range using Kruskal-Wallis test. Results: Amount of CFUs was higher in decompensated patients (P = .025). The most isolated species in the sample was C. albicans (66.1%) followed by C. glabrata (20.7%). Predominant species in compensated patients was C. tropicalis, while in decompensated patients, were C. albicans and C. krusei. There was an inverse association between the value of HbA1c and salivary pH. At lower values of salivary pH, more diversity and quantity of Candida yeasts was observed. Conclusions: Changes in oral microbiota are related to changes in the salivary pH associated with metabolic control of type 2 diabetes.
ADENOMATOID ODONTOGENIC TUMOR. A CLINICAL-PATHOLOGIC STUDY OF 27 CASES. DIANA GABRIELA MORI ALIAGA, ALONDRA HORMAZABAL HEVIA, IRIS ESPINOZA GOMEZ SANTANDER, ANA ORTEGA PINTO, FERNAN