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Evaluation of transgenic fish for detecting in vivo mutations
Marine Environmental Research 50 (2000) 591±592 www.elsevier.com/locate/marenvrev
Abstracts
Evaluation of transgenic ®sh for detecting in vivo mutations R. Winn a, M. Norris a, K. Brayer a, J. Kind b, C. Dallas b a
Warnell School of Forest Resources, University of Georgia, Athens GA 30606, USA b College of Pharmacy, University of Georgia, Athens, GA 30606, USA
Abstract Transgenic medaka and mummichog were developed that carry multiple copies of either lambda bacteriophage, or plasmid vectors that harbor target genes (lacZ, lacI, or cII) for mutation detection. The common approach used in the three mutation assays entails exposing ®sh to a potential mutagen, followed by recovering the vector from the genomic DNA, and quantifying mutations in the target genes via indicator bacteria. A number of features of the ®sh mutation assays were evaluated related to their potential utility in genotoxicology. The vectors were recovered from transgenic ®sh genomic DNA with eciencies that surpassed those of transgenic mice that carry identical vectors. The spontaneous mutation frequencies of the three target genes obtained from the ®sh were comparable to one another, as well as, to transgenic mice. The induction of mutations following chemical mutagen exposure indicated predictable and sensitive responses. Individual mutations were examined to provide spontaneous and induced mutational spectra. These analyses demonstrate that the fundamental requirements for mutation detection using transgenic ®sh have been met. PII: S0141-1136(00)00242-7
DNA adducts in northern pike (Esox lucius) exposed to benzo[a]pyrene, benzo[k]¯ouranthene and 7H-dibenzo[c,g]carbazole G. Ericson, E. Noaksson, L. Balk Laboratory for Aquatic Ecotoxicology, Institute of Applied Environmental Research, Stockholm University, S-611 82 NykoÈping, Sweden
Abstracts
Evaluation of transgenic ®sh for detecting in vivo mutations R. Winn a, M. Norris a, K. Brayer a, J. Kind b, C. Dallas b a
Warnell School of Forest Resources, University of Georgia, Athens GA 30606, USA b College of Pharmacy, University of Georgia, Athens, GA 30606, USA
Abstract Transgenic medaka and mummichog were developed that carry multiple copies of either lambda bacteriophage, or plasmid vectors that harbor target genes (lacZ, lacI, or cII) for mutation detection. The common approach used in the three mutation assays entails exposing ®sh to a potential mutagen, followed by recovering the vector from the genomic DNA, and quantifying mutations in the target genes via indicator bacteria. A number of features of the ®sh mutation assays were evaluated related to their potential utility in genotoxicology. The vectors were recovered from transgenic ®sh genomic DNA with eciencies that surpassed those of transgenic mice that carry identical vectors. The spontaneous mutation frequencies of the three target genes obtained from the ®sh were comparable to one another, as well as, to transgenic mice. The induction of mutations following chemical mutagen exposure indicated predictable and sensitive responses. Individual mutations were examined to provide spontaneous and induced mutational spectra. These analyses demonstrate that the fundamental requirements for mutation detection using transgenic ®sh have been met. PII: S0141-1136(00)00242-7
DNA adducts in northern pike (Esox lucius) exposed to benzo[a]pyrene, benzo[k]¯ouranthene and 7H-dibenzo[c,g]carbazole G. Ericson, E. Noaksson, L. Balk Laboratory for Aquatic Ecotoxicology, Institute of Applied Environmental Research, Stockholm University, S-611 82 NykoÈping, Sweden