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Gel Electrophoresis of Nucleic Acids: A Practical Approach
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and its effect on their hydration and configuration. Two articles deal with polymeric or polyionic autolysis. Seventeeen articles discuss conformations of protein polypeptides and polynucleic acids, their dynamics, their fluctuations and kinetics of their changes. Ten articles discuss membrane structure potential and ionic conductance. The subject of five other articles is membrane energetics, including chemoreception energy transduction and redox reaction. The last four articles in the morphogenesis section discuss electrical field modulated membrane structure, selfassembly and stability. It is a pity that a very long time has elapsed between the writing of the manuscripts and the publication. This does not make the book obsolete, but it is not exactly up to date. I. MILLER Weizmann Institute, Membrane Laboratory, Rehovot
Gel Electrophoresis of Nucleic Acids: A Practical Approach. D. Rickwood and B. Hames. (Editors). IRL Press, Oxford, England, 1982, xv + 242 pp., 70 figs., 15 X 23 cm, B.50, $18.00. This comprehensive working manual dealing with one of the most important separation methods in biochemistry contains chapters from different authors on the following subjects: (1) gel electrophoresis of RNA; (2) electrophoresis of DNA, (3) two-dimensional gel electrophoresis of nucleic acids; (4) DNA sequencing; (5) RNA sequencing; (6) electrophoresis of nucleoproteins. Appendix I deals with nucleic acid molecular weight markers, and Appendix II with suppliers (from different authors) of specialist items for electrophoresis. In every case very detailed descriptions of the horizontal and vertical chamber, the preparation of gels, electrophoresis conditions and the further possibilities of analysis are given. Another important point deals with the good recovery rate, especially for sophisticated techniques. Appendix III deals with examples of application for DNA destruction fragments, and nucleosomes show the sensitivity of this method. The lists of sizes of destruction fragments and of molecular weight markers for gel electrophoresis of RNA in Appendix I are valuable for tests. This book is as useful as its companion volume, Gel Electrophoresis of Proteins, by the same authors. H. BERG Institute of Microbiology, Academy of Sciences, Jena
and its effect on their hydration and configuration. Two articles deal with polymeric or polyionic autolysis. Seventeeen articles discuss conformations of protein polypeptides and polynucleic acids, their dynamics, their fluctuations and kinetics of their changes. Ten articles discuss membrane structure potential and ionic conductance. The subject of five other articles is membrane energetics, including chemoreception energy transduction and redox reaction. The last four articles in the morphogenesis section discuss electrical field modulated membrane structure, selfassembly and stability. It is a pity that a very long time has elapsed between the writing of the manuscripts and the publication. This does not make the book obsolete, but it is not exactly up to date. I. MILLER Weizmann Institute, Membrane Laboratory, Rehovot
Gel Electrophoresis of Nucleic Acids: A Practical Approach. D. Rickwood and B. Hames. (Editors). IRL Press, Oxford, England, 1982, xv + 242 pp., 70 figs., 15 X 23 cm, B.50, $18.00. This comprehensive working manual dealing with one of the most important separation methods in biochemistry contains chapters from different authors on the following subjects: (1) gel electrophoresis of RNA; (2) electrophoresis of DNA, (3) two-dimensional gel electrophoresis of nucleic acids; (4) DNA sequencing; (5) RNA sequencing; (6) electrophoresis of nucleoproteins. Appendix I deals with nucleic acid molecular weight markers, and Appendix II with suppliers (from different authors) of specialist items for electrophoresis. In every case very detailed descriptions of the horizontal and vertical chamber, the preparation of gels, electrophoresis conditions and the further possibilities of analysis are given. Another important point deals with the good recovery rate, especially for sophisticated techniques. Appendix III deals with examples of application for DNA destruction fragments, and nucleosomes show the sensitivity of this method. The lists of sizes of destruction fragments and of molecular weight markers for gel electrophoresis of RNA in Appendix I are valuable for tests. This book is as useful as its companion volume, Gel Electrophoresis of Proteins, by the same authors. H. BERG Institute of Microbiology, Academy of Sciences, Jena