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stocks of Zea mays L, plant DNA variation and stress, emergence of new genes, organization of mutant genes in mouse t-haplotypes, genetic analysis of morphogenesis, cell lineage and cell interactions in the determination of developmental cell fates, structures and regulated transcription of DIRS-1, a novel Dictyostelium discoideum transposable element, developmental constraints and evolutionary saltations; intraspecies genomic variation, structure and function of the human retroviruses, the T-DNA genes of Agrobacterium plasmids appear to be of a complex evolutionary origin). G.D. Hanes and S.J. Higgins (Eds.): Nucleic Acid Hybridisation: A Practical Approach. IRL Press, Oxford, Washington, DC, 1985, xv + 245 pp. $40.00/f22.00 softbound, ($25.00/f14.00 hardbound), ISBN O-947946-23-3. [ 14 contributors; eight chapters; four appendices. Hybridization strategy (R.J. Britten and E.H. Davidson); Preparation of probes (J.E. Arrand); solution hybridization (B.D. Young and M.L.M. Anderson); filter hybridization (M.L.M. Anderson and B.D. Young); hybridization of recombinant DNA (P.J. Mason and J.G. Williams); hybridization of RNA (J.G. Williams and P.J. Mason); electron microscopic visualization of hybrids (P. Oudet and C. Schatz); in situ hybridization (M.L. Pardue). Appendices : restriction enzymes (R.J. Roberts); nucleic acid size markers (S.J. Minter, P.G. Sealey and J. Arrand); computer analysis of hybridization data (B.D. Young and M.L.M. Anderson); suppliers of specialized items]. D.R. Helinski, S.N. Cohen, D.B. Clewell, D.A. Jackson and A. Hollaender (Eds.): Plasmids in Bacteria. Plenum Press, New York and London, 1985, xv + 995 pp. ($110.00) ISBN o-306-41901-7.
[227 authors of 59 articles; 102 poster abstracts. Structure and evolution of plasmids (plasmids as organisms; structure and function; transposition in bacteria; transcriptional elements; IS 1; TnZ271; repeated sequences; mercury resistance transposon Tn813; plasmids in unusual systems). Replication, incompatibility and partition (the pi initiation protein; ori of E. coli; ori of adv; broad-host-range plasmid; copy number control; partition functions of Pl, P7 and F miniplasmids; replication regulon; control of chromosome replication; RNA 1 mutants of ColEl; DNA-protein interaction at ori; transcription initiation on TOL plasmid). Plasmid transfer (linear plasmids with terminal inverted repeats; conjugal plasmid transfer; stable inheritance of mini-F plasmid; sex pheromones; general genetic recombination; origin of DNA transfer; genetics of clindamycin resistance in Bacteroides; plasmid DNA primase; sex plasmids in Streptomyces). Specialized functions, structure and evolution (shuttle vector; degradation of chlorinated aromatic compounds; mer operon; iron uptake systems; iron sequestering; exotoxin A; hemolysin; virulence plasmid; plant cell transformation; regulation of CAT; larval toxin). Posters (replication, partition, specialized functions, structure and evolution, transfer, vectors)]. M.L. Hooper: Mammalian Cell Genetics. Wiley and Sons, New York, 1985, xiv + 175 pp. ($49.95) ISBN o-471-89201-7. (The cultured mammalian cell; selective techniques; cell variants; cell heterokaryons and hybrids; molecular somatic cell genetics; cell genetics and the germline; a rather superficial monograph with 817 references but without authors index; many important earlier references are missing; not all areas of the field are adequately covered). Prepared by A.J. Podhajska.