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Genital HPVs in premalignant and malignant skin lesions
S196
ESDR I JSID I SID Abstracts
1174
1171 THE POSSIBLE ROLES OF STAPHYLOCOCCAL ENTEROTOXINS ON ATOPIC DERMATITIS. Joii Tada, Yoshiko Morishita, Yoichiro Toi. Hisanori Akivama, Jir6 Arata and Hiroshi Kate*, Department of Dermatology, Okayama University Medical School, Okayama, Japan, and *Nippon DPC Corporation, Chiba, Japan. Skin lesions of atopic dermatitis (AD) are densely colonized with Staphylococcus aweus. We investigated the presence of specific IgE antibodies to staphylococcal enterotoxins (SEA, SEB) in AD patients, the adhesion molecule-expression of keratinocytes in organ cultured normal skin treated with SEA or SEB, and the localization of SEB in the lesional skin. The amount of anti-SEA and-SEB IgE antibodies were measured by liquid-phase enzyme immunoassay in 149 patients. Eighty-one (54.4%) patients had specific IgE antibosdies against SEA and 97 (65.1%) had against SEB. Only three of 28 control subjects had specific antibodies to SEA or SEB. In organ cultured skin incubated with SEA or SEB, the epidermal keratinocytes expressed HLA-DR and ICAM-1. The localization of SEB in the lesional skin was detected on the dermal eosinophils by double immunofluorescence staining. These results suggest that staphylococcal enterotoxins may play important roles, through IgE-mediated response, in the exacerbation and prolongation of AD, and furthermore infiltrated eosinophils may play an impotant role in its pathogen&c process.
1172
1175
BFURGDO~~~PCRwrraDlerrERENTP~SFROMS~BlOPSIESANDURME COMPABBDTOCUI.TUBEANDSEROLOGYMSI‘INBOBBELIOSIS. He~deloreHcbmann, Hxald BmckLwer, Bea,e Heuser and Svlvm Bre,tsclme,der. Dept ofDermatology and Allergy, Techmcal University Mdnchen, Germany Skm biouws of 46 oattents wth skm tarrehasm tewthema nwmns IEMl, muhmle ewthemata
GENITAL HPVs IN PREMALIGNANT AND MALIGNANT SKlN LESIONS, m Mever. Institute Of Immunology, Pathology and Molecular Ecology, Hamburg, Department of Dermatology, University of I&l, Germany. Based on the as~ww.tmn wdh chntcal leaons human pap~llomavrws (HPV) are usualiy grouped mto cutanecms and mucasal or gemtal HPV types. While the etmloglc role of HPV infection for gemtal dysplastic and neoplastic lesions 1s generally accepted for several years, the assocmtion of HPV mfectmn with development of non-melanoma skm cancers was considered only recently. Many of the HPV types detected m s$n tunmrs belong to the group of mucosal HPVs, suggestmg a less stnngent tlssw tropism of these HPV types. To mvest@e the pathogentc role of gemtal HPVs m skm t~nmrs, affected and nwmal skm t~ssucs of patxnts wth premalignant (M. Bowen, Actmlc Keratosis) and malignant skm tumxs (Basal Cell Carctnoma - BCC, Squamous Cell Carcmoma SCC) were analyzed for the presence of viral DNA. HPV DNA was detected usmg Ll-consensus pnmers m standard and nested PCR assays. Typmg of amphtied HPV DNA was performed by RFLP analysts and subsequent hybrldzatmn wth a gmenc ol,gonucleot,de probe. HPV DNA was detected m 40% (Actmx Ker&os,s), 30% (M. Bowcn), 37% (BCC) and 38% (SCC) ofspecimens. In contrast, only one of I4 (7%) normal skm specmxns was HP” DNA pcwbve (abmned from a pabent wth SCC). The spectrum af HPV types ldentlf,ed comprised HP” types 6, 11, 16, 31,35,39,40,58 and 70. The presence of oncogenic HPVs (HPV 16, II, 35,39,58) ma subset of specimens and the nwre frequent detectmn of gemtal HPVa m affected t,ssws as compared to normal skm spemmens may indicate involvement af these vimses m tumongenesis in mdwldual cases.
1176 EMBEDDED BtopsI~s OF KAposI's SARCOMA OF HWSER~POSIT~ -SERONEGATNEPATlENTSBYPOLYMERASECHAINREACTION G. Bezold”,
G. Mesw’,
AND
U. Puchta.‘, R.U. Peter’, P. Kind’, C. Sander’
’ Department of Dermatology, ’ Department of Dermatology,
University of Ulm, D-8908 I Ulm Ludwig-Maximilians-University, D-80337 Munich
Human herpes virus 8 (HHVS) DNA has repeatedly been reported in Kaposi’s sarcoma (KS) as a causative agent. The purpose of this study was the quantify the HHV8 viral load in KS of patients seropositive and semnegative for HlV. As KS in HIV seronegative patients IS rather rare, 27 archived paraffin-embedded samples were used After DNA extraction HHVS DNA fragments (233 bp) were competitively amplified by PCR using an internal quantitation standard (mimic). D~wstream primers were labeled with digoxigenin. Serial dilutions of HHV8 and mimic PCR products were quantified by ELISA. To wrrect for different extent of DNA degradatton, i.e., different attmunts of amplifiable DNA, also human E globin fragments of similar length (268 bp) were quantitated in every sample. Results were expressed as HEW8 copies per 1000 R-globin copier. In 13 biopsies fmm HIV-seropositive patlentS 14.8 i 19.6 HHV8 per 1000 D-globin-copies were found versus 18.0 * 23.5 in I4 biopsies from HIV-seronegatlve patxnts, mean f standard deviation. The difference was not significant (Mann Whithney Rank Sum Test). The amwnt of HHVS in KS is quite low as only few cells are infected. The same viral load in HIV-negative and HIV-positive samples was quite surprising. More studies have to be conducted to further investigate the importance of HHV8 and its viral load in Kawsi s sarcoma. and ts being discussed
THE DIAGNOSTIC SlGNlFICANCE OF EPSTEIN-BARR VIRUS INFECTION IN SUBCUTANEOUS LYMPHOMA: A COMPARATIVE STUDY WITH PANNICULITIC DISORDERS. Miki&&&&Keili.Lwatsuki. and Fumio Kaneko. Department of Dermatology, Fukushbna Medical College, Fukushima, Japan Cytophagic hisfiocytic panniculitis (CHP) is characterized by lobular panniculitis associated with hemophagocytosis, and its histologic features are often indistinguishable from those of an early lesion of subcutaneous lymphoma. Recently, latent Epstein-Barr virus (EBV) infection has been found in various types of cutaneous lymphoproliferative disorders including subcutaneous lymphoma. We studied the diagnostic significance of latent EBV infection in malignant and benign panniculitic disorders by means of in situ hybridization (ISH) and polymerase chain reaction(PCR). Both EBV genomes and EBV-encoded small RNAs (EBERs) were present in 3 of 4 patients with subcutaneous lymphomas. These patients presented with subcutaneous panniculitic lesions containing phagocytizing histiocytes, ie. beanbag cells, to various degrees. Despite the presence of hemophagocytic figures in the cutaneous lesions, none of 5 patients with CHP successfully treated with conventional medications had latent EBV infection. Neither EBV genomes nor EBERs were detected in 5 cases with erythema nodosum, and 3 cases with lupus erythematosus profundus. These findings indicate that latent EBV infection is more frequently involved in subcutaneous lymphoma associated with hemophagocytosis,
which
might
be distinct
from
benign
panniculitis.
ESDR I JSID I SID Abstracts
1174
1171 THE POSSIBLE ROLES OF STAPHYLOCOCCAL ENTEROTOXINS ON ATOPIC DERMATITIS. Joii Tada, Yoshiko Morishita, Yoichiro Toi. Hisanori Akivama, Jir6 Arata and Hiroshi Kate*, Department of Dermatology, Okayama University Medical School, Okayama, Japan, and *Nippon DPC Corporation, Chiba, Japan. Skin lesions of atopic dermatitis (AD) are densely colonized with Staphylococcus aweus. We investigated the presence of specific IgE antibodies to staphylococcal enterotoxins (SEA, SEB) in AD patients, the adhesion molecule-expression of keratinocytes in organ cultured normal skin treated with SEA or SEB, and the localization of SEB in the lesional skin. The amount of anti-SEA and-SEB IgE antibodies were measured by liquid-phase enzyme immunoassay in 149 patients. Eighty-one (54.4%) patients had specific IgE antibosdies against SEA and 97 (65.1%) had against SEB. Only three of 28 control subjects had specific antibodies to SEA or SEB. In organ cultured skin incubated with SEA or SEB, the epidermal keratinocytes expressed HLA-DR and ICAM-1. The localization of SEB in the lesional skin was detected on the dermal eosinophils by double immunofluorescence staining. These results suggest that staphylococcal enterotoxins may play important roles, through IgE-mediated response, in the exacerbation and prolongation of AD, and furthermore infiltrated eosinophils may play an impotant role in its pathogen&c process.
1172
1175
BFURGDO~~~PCRwrraDlerrERENTP~SFROMS~BlOPSIESANDURME COMPABBDTOCUI.TUBEANDSEROLOGYMSI‘INBOBBELIOSIS. He~deloreHcbmann, Hxald BmckLwer, Bea,e Heuser and Svlvm Bre,tsclme,der. Dept ofDermatology and Allergy, Techmcal University Mdnchen, Germany Skm biouws of 46 oattents wth skm tarrehasm tewthema nwmns IEMl, muhmle ewthemata
GENITAL HPVs IN PREMALIGNANT AND MALIGNANT SKlN LESIONS, m Mever. Institute Of Immunology, Pathology and Molecular Ecology, Hamburg, Department of Dermatology, University of I&l, Germany. Based on the as~ww.tmn wdh chntcal leaons human pap~llomavrws (HPV) are usualiy grouped mto cutanecms and mucasal or gemtal HPV types. While the etmloglc role of HPV infection for gemtal dysplastic and neoplastic lesions 1s generally accepted for several years, the assocmtion of HPV mfectmn with development of non-melanoma skm cancers was considered only recently. Many of the HPV types detected m s$n tunmrs belong to the group of mucosal HPVs, suggestmg a less stnngent tlssw tropism of these HPV types. To mvest@e the pathogentc role of gemtal HPVs m skm t~nmrs, affected and nwmal skm t~ssucs of patxnts wth premalignant (M. Bowen, Actmlc Keratosis) and malignant skm tumxs (Basal Cell Carctnoma - BCC, Squamous Cell Carcmoma SCC) were analyzed for the presence of viral DNA. HPV DNA was detected usmg Ll-consensus pnmers m standard and nested PCR assays. Typmg of amphtied HPV DNA was performed by RFLP analysts and subsequent hybrldzatmn wth a gmenc ol,gonucleot,de probe. HPV DNA was detected m 40% (Actmx Ker&os,s), 30% (M. Bowcn), 37% (BCC) and 38% (SCC) ofspecimens. In contrast, only one of I4 (7%) normal skm specmxns was HP” DNA pcwbve (abmned from a pabent wth SCC). The spectrum af HPV types ldentlf,ed comprised HP” types 6, 11, 16, 31,35,39,40,58 and 70. The presence of oncogenic HPVs (HPV 16, II, 35,39,58) ma subset of specimens and the nwre frequent detectmn of gemtal HPVa m affected t,ssws as compared to normal skm spemmens may indicate involvement af these vimses m tumongenesis in mdwldual cases.
1176 EMBEDDED BtopsI~s OF KAposI's SARCOMA OF HWSER~POSIT~ -SERONEGATNEPATlENTSBYPOLYMERASECHAINREACTION G. Bezold”,
G. Mesw’,
AND
U. Puchta.‘, R.U. Peter’, P. Kind’, C. Sander’
’ Department of Dermatology, ’ Department of Dermatology,
University of Ulm, D-8908 I Ulm Ludwig-Maximilians-University, D-80337 Munich
Human herpes virus 8 (HHVS) DNA has repeatedly been reported in Kaposi’s sarcoma (KS) as a causative agent. The purpose of this study was the quantify the HHV8 viral load in KS of patients seropositive and semnegative for HlV. As KS in HIV seronegative patients IS rather rare, 27 archived paraffin-embedded samples were used After DNA extraction HHVS DNA fragments (233 bp) were competitively amplified by PCR using an internal quantitation standard (mimic). D~wstream primers were labeled with digoxigenin. Serial dilutions of HHV8 and mimic PCR products were quantified by ELISA. To wrrect for different extent of DNA degradatton, i.e., different attmunts of amplifiable DNA, also human E globin fragments of similar length (268 bp) were quantitated in every sample. Results were expressed as HEW8 copies per 1000 R-globin copier. In 13 biopsies fmm HIV-seropositive patlentS 14.8 i 19.6 HHV8 per 1000 D-globin-copies were found versus 18.0 * 23.5 in I4 biopsies from HIV-seronegatlve patxnts, mean f standard deviation. The difference was not significant (Mann Whithney Rank Sum Test). The amwnt of HHVS in KS is quite low as only few cells are infected. The same viral load in HIV-negative and HIV-positive samples was quite surprising. More studies have to be conducted to further investigate the importance of HHV8 and its viral load in Kawsi s sarcoma. and ts being discussed
THE DIAGNOSTIC SlGNlFICANCE OF EPSTEIN-BARR VIRUS INFECTION IN SUBCUTANEOUS LYMPHOMA: A COMPARATIVE STUDY WITH PANNICULITIC DISORDERS. Miki&&&&Keili.Lwatsuki. and Fumio Kaneko. Department of Dermatology, Fukushbna Medical College, Fukushima, Japan Cytophagic hisfiocytic panniculitis (CHP) is characterized by lobular panniculitis associated with hemophagocytosis, and its histologic features are often indistinguishable from those of an early lesion of subcutaneous lymphoma. Recently, latent Epstein-Barr virus (EBV) infection has been found in various types of cutaneous lymphoproliferative disorders including subcutaneous lymphoma. We studied the diagnostic significance of latent EBV infection in malignant and benign panniculitic disorders by means of in situ hybridization (ISH) and polymerase chain reaction(PCR). Both EBV genomes and EBV-encoded small RNAs (EBERs) were present in 3 of 4 patients with subcutaneous lymphomas. These patients presented with subcutaneous panniculitic lesions containing phagocytizing histiocytes, ie. beanbag cells, to various degrees. Despite the presence of hemophagocytic figures in the cutaneous lesions, none of 5 patients with CHP successfully treated with conventional medications had latent EBV infection. Neither EBV genomes nor EBERs were detected in 5 cases with erythema nodosum, and 3 cases with lupus erythematosus profundus. These findings indicate that latent EBV infection is more frequently involved in subcutaneous lymphoma associated with hemophagocytosis,
which
might
be distinct
from
benign
panniculitis.