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Greenlandic pregnant women: Food intake and Serum Persistent Organic Pollutants
S96
Abstracts / Toxicology Letters 238S (2015) S56–S383
P03-027 Occupational exposure to aflatoxin B1 in poultry slaughterhouses S. Viegas 1,2,∗ , L. Veiga 3 , A. Almeida 3 , M. Dos Santos 1 1
ESTeSL-IPL, Environmental Health RG, Lisboa, Portugal Centro de Investigac¸ão em Saúde Pública (CISP/ENSP/UNL), Lisbon, Portugal 3 ESTeSL-IPL, Lisbon, Portugal 2
Exposure to mycotoxins is not usually identified as a risk factor present in occupational settings. This is probably due to the inexistence of limits regarding concentration of airborne mycotoxins and, also, due to the fact that these compounds are rarely monitored in occupational environments. Recently, published research work claimed attention to occupational exposure to aflatoxin B1 (AFB1 ) in poultry and swine production. Considering previous studies, it was developed a study in Portugal aiming to assess occupational exposure to AFB1 in one poultry slaughterhouse. Occupational exposure assessment started to measure AFB1 in workers serum by enzyme-linked immunosorbent assay (ELISA). Thirty workers from the slaughterhouse were enrolled in this study. A control group (n = 30) was also considered in order to compare with the AFB1 background levels for the Portuguese population. In the workers group, 14 individuals (47%) showed detectable levels of AFB1 with values ranging from 1.1 ng/ml to 4 ng/ml with a mean value of 1.8 ± 0.8 ng/ml. All of the controls showed values below the method’s detection limit (LOD = 1 ng/ml). Results reveal occupational exposure to AFB1 and exposure is probably occurring by inhalation. Possibly there are some workplaces that involve specific conditions that promote exposure such as: high particulate matter contamination and suitable temperatures for fungal development and mycotoxins production.
Results: A trend of higher intake of marine mammals in the East and North was reflected by a higher n-3/n-6 fatty acids ratio. The East participants tended also to have higher intake of terrestrial species. A significant higher seabird intake was seen for pregnant women in the Mid-West region. Significant regional differences were found for blood concentrations of PCBs, OCPs, PFAS and mercury, with higher levels in the North and East regions. POPs were positively correlated to the n-3/n-6 ratio. The PFAS were significantly associated with the PCBs and OCPs in most regions. Moreover, in the North region the PFAS were associated with both selenium and mercury. No significant regional difference was observed for PBDEs. Discussion: The regional difference of POPs and mercury levels were related to marine food intake and plasma n-3/n-6 ratio. Compared to earlier reports of pregnant women, decreased levels of legacy POPs, Hg, Pb and perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA) were observed. But the level of other PFAS congeners such as perfluorohexane sulfonate (PFHxS), perfluorononanoic acid (PFNA) sustained. The detection of POPs and heavy metals in the maternal blood indicate that the fetus is exposed to these compounds that can influence the fetus development. The levels of contaminants in pregnant Inuit women will be compared with levels observed for Danish, Norwegian and Chinese pregnant women in the same time period. http://dx.doi.org/10.1016/j.toxlet.2015.08.319
P03-029 Greenlandic pregnant women: Serum Persistent Organic Pollutants interfere with sex hormone receptor transactivities E.C. Bonefeld-Jørgensen ∗ , L.S. Kjeldsen, C.B. Olesen, M. Long
http://dx.doi.org/10.1016/j.toxlet.2015.08.318
Aarhus University, Centre for Arctic Health & Unit of Cellular and Molecular Toxicology, Aarhus, Denmark
P03-028 Greenlandic pregnant women: Food intake and Serum Persistent Organic Pollutants
Background: High concentrations of persistent organic pollutants (POPs) in the Arctic population have previously been associated with the intake of traditional marine food. Several studies have documented endocrine-disrupting effects of POPs and their impact on fetal and child developmental health. Aim: We assessed the geographical variance in POP levels and the effect of the lipophilic POPs on sex hormone receptor function in Greenlandic pregnant women. Methods: Blood samples and questionnaire data were collected from 189 pregnant women during 2010–2011 in five Greenlandic regions (North, Disco Bay, Mid-West, South and East). The lipophilic POPs, including organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), were extracted from serum using solid phase extraction (SPE) followed by high performance liquid chromatography (HPLC). The SPE-HPLC F1 serum fraction, free of endogenous hormones, containing the actual lipophilic POP mixture were analyzed for the capacity to affect estrogen- (ER), androgen (AR)-receptor transactivities using reporter-gene assays in mammalian cell culture systems. Results: Pregnant women living in the North and the East region had higher serum levels of PCBs and OCPs. No significant regional difference was observed for PBDEs. In overall approximately 50% of the lipophilic POP-F1 extracts induced the ER and the AR transactivities. Furthermore, upon co-exposure with the potential receptor ligands, 17% of the POP-F1 extracts further increased the 17estradiol induced ER-transactivity, whereas 60% of the POP-F1 extracts antagonized the dihydrotestosterone induced AR transactivity. A tendency of inverse correlation between serum POP levels
M. Long 1,∗ , A.-K.S. Knudsen 1 , H.S. Pedersen 2 , E.C. Bonefeld-Jørgensen 1 1 Aarhus University, Centre for Arctic Health & Unit of Cellular and Molecular Toxicology, Aarhus, Denmark 2 Primary Health Care Center, Nuuk, Greenland
Background: The Greenlandic Inuit have high blood concentrations of environmental persistent organic pollutants (POPs), reported to be associated with age, smoking and intake of marine food. Studies have indicated that exposure to POPs during pregnancy can have adverse effect on fetal and child developmental health. Purpose: To assess the geographical differences in the exposure to POPs of pregnant women in Greenland. Methods: The 207 pregnant women were, for this ACCEPT substudy, enrolled during 2010–11 and a few from 2013. Questionnaire data and blood samples were collected from the pregnant women living in five Greenlandic regions (North, Disco Bay, Mid-West, South, East). Blood samples were analyzed for 11 organochlorine pesticides (OCPs), 14 polychlorinated biphenyls (PCBs), 5 polybrominated diphenyl ethers (PBDEs), 15 perfluoroalkylated substances (PFAS) and 63 metals.
Abstracts / Toxicology Letters 238S (2015) S56–S383
P03-027 Occupational exposure to aflatoxin B1 in poultry slaughterhouses S. Viegas 1,2,∗ , L. Veiga 3 , A. Almeida 3 , M. Dos Santos 1 1
ESTeSL-IPL, Environmental Health RG, Lisboa, Portugal Centro de Investigac¸ão em Saúde Pública (CISP/ENSP/UNL), Lisbon, Portugal 3 ESTeSL-IPL, Lisbon, Portugal 2
Exposure to mycotoxins is not usually identified as a risk factor present in occupational settings. This is probably due to the inexistence of limits regarding concentration of airborne mycotoxins and, also, due to the fact that these compounds are rarely monitored in occupational environments. Recently, published research work claimed attention to occupational exposure to aflatoxin B1 (AFB1 ) in poultry and swine production. Considering previous studies, it was developed a study in Portugal aiming to assess occupational exposure to AFB1 in one poultry slaughterhouse. Occupational exposure assessment started to measure AFB1 in workers serum by enzyme-linked immunosorbent assay (ELISA). Thirty workers from the slaughterhouse were enrolled in this study. A control group (n = 30) was also considered in order to compare with the AFB1 background levels for the Portuguese population. In the workers group, 14 individuals (47%) showed detectable levels of AFB1 with values ranging from 1.1 ng/ml to 4 ng/ml with a mean value of 1.8 ± 0.8 ng/ml. All of the controls showed values below the method’s detection limit (LOD = 1 ng/ml). Results reveal occupational exposure to AFB1 and exposure is probably occurring by inhalation. Possibly there are some workplaces that involve specific conditions that promote exposure such as: high particulate matter contamination and suitable temperatures for fungal development and mycotoxins production.
Results: A trend of higher intake of marine mammals in the East and North was reflected by a higher n-3/n-6 fatty acids ratio. The East participants tended also to have higher intake of terrestrial species. A significant higher seabird intake was seen for pregnant women in the Mid-West region. Significant regional differences were found for blood concentrations of PCBs, OCPs, PFAS and mercury, with higher levels in the North and East regions. POPs were positively correlated to the n-3/n-6 ratio. The PFAS were significantly associated with the PCBs and OCPs in most regions. Moreover, in the North region the PFAS were associated with both selenium and mercury. No significant regional difference was observed for PBDEs. Discussion: The regional difference of POPs and mercury levels were related to marine food intake and plasma n-3/n-6 ratio. Compared to earlier reports of pregnant women, decreased levels of legacy POPs, Hg, Pb and perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA) were observed. But the level of other PFAS congeners such as perfluorohexane sulfonate (PFHxS), perfluorononanoic acid (PFNA) sustained. The detection of POPs and heavy metals in the maternal blood indicate that the fetus is exposed to these compounds that can influence the fetus development. The levels of contaminants in pregnant Inuit women will be compared with levels observed for Danish, Norwegian and Chinese pregnant women in the same time period. http://dx.doi.org/10.1016/j.toxlet.2015.08.319
P03-029 Greenlandic pregnant women: Serum Persistent Organic Pollutants interfere with sex hormone receptor transactivities E.C. Bonefeld-Jørgensen ∗ , L.S. Kjeldsen, C.B. Olesen, M. Long
http://dx.doi.org/10.1016/j.toxlet.2015.08.318
Aarhus University, Centre for Arctic Health & Unit of Cellular and Molecular Toxicology, Aarhus, Denmark
P03-028 Greenlandic pregnant women: Food intake and Serum Persistent Organic Pollutants
Background: High concentrations of persistent organic pollutants (POPs) in the Arctic population have previously been associated with the intake of traditional marine food. Several studies have documented endocrine-disrupting effects of POPs and their impact on fetal and child developmental health. Aim: We assessed the geographical variance in POP levels and the effect of the lipophilic POPs on sex hormone receptor function in Greenlandic pregnant women. Methods: Blood samples and questionnaire data were collected from 189 pregnant women during 2010–2011 in five Greenlandic regions (North, Disco Bay, Mid-West, South and East). The lipophilic POPs, including organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), were extracted from serum using solid phase extraction (SPE) followed by high performance liquid chromatography (HPLC). The SPE-HPLC F1 serum fraction, free of endogenous hormones, containing the actual lipophilic POP mixture were analyzed for the capacity to affect estrogen- (ER), androgen (AR)-receptor transactivities using reporter-gene assays in mammalian cell culture systems. Results: Pregnant women living in the North and the East region had higher serum levels of PCBs and OCPs. No significant regional difference was observed for PBDEs. In overall approximately 50% of the lipophilic POP-F1 extracts induced the ER and the AR transactivities. Furthermore, upon co-exposure with the potential receptor ligands, 17% of the POP-F1 extracts further increased the 17estradiol induced ER-transactivity, whereas 60% of the POP-F1 extracts antagonized the dihydrotestosterone induced AR transactivity. A tendency of inverse correlation between serum POP levels
M. Long 1,∗ , A.-K.S. Knudsen 1 , H.S. Pedersen 2 , E.C. Bonefeld-Jørgensen 1 1 Aarhus University, Centre for Arctic Health & Unit of Cellular and Molecular Toxicology, Aarhus, Denmark 2 Primary Health Care Center, Nuuk, Greenland
Background: The Greenlandic Inuit have high blood concentrations of environmental persistent organic pollutants (POPs), reported to be associated with age, smoking and intake of marine food. Studies have indicated that exposure to POPs during pregnancy can have adverse effect on fetal and child developmental health. Purpose: To assess the geographical differences in the exposure to POPs of pregnant women in Greenland. Methods: The 207 pregnant women were, for this ACCEPT substudy, enrolled during 2010–11 and a few from 2013. Questionnaire data and blood samples were collected from the pregnant women living in five Greenlandic regions (North, Disco Bay, Mid-West, South, East). Blood samples were analyzed for 11 organochlorine pesticides (OCPs), 14 polychlorinated biphenyls (PCBs), 5 polybrominated diphenyl ethers (PBDEs), 15 perfluoroalkylated substances (PFAS) and 63 metals.