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Heat shock proteins in cultured human skin fibroblasts
573
574
DIPEPTI9YL OF x-pro PURIFICATION AMINOPEPTIDASE FROM RAT SKIN HIPOSHI SHUNJI NAKANO, MASAHIR" KUSUHARA, HACilISUKA AND YOICHIRO SASAI University of Dermatoloqy, Kurume Department School of Med1clnc, Kurume A" enzyme with the speclflclty of a X-?rO purlfief+ fron rllpfptlclyl amlnopeptldase was rat Si;lii. 7-iGly-Pro)-4-methylCOumarin~mide was used for deternlnation of (Gly-Pro-YCA) has P pH The enzyme actLvity. rhe enzyme weight r,pt3m1,m of 5.0 to 5.5 and a molecular The enzyme t3y gel flltratio". aholit llO,Ofli? actl\Tlty 1s strongly inhlhited by the serl"e SETI protease InhIbitor DFP and PMSF. NEM a"d functlo" of ?Llave no effect on activity. The of enzyme mlqht hr the degradation this i-ub?tance r.
‘THE ELE;VATED LEVEL OF KED BLOOD CELL SUPEROXIDE ANION IN PATIENTS WITH SYSTEMIC SCLEROSISISSI SI1ICEO KONDOU, KAZUE SUGAYA, AlSUKO SEKI?JE. HlhARU SHIGEO NISHIYPSV1A, KL’NIE -* ETO, HARUE ARAI, Departments of Dermatology and Molecular Biology*, ti~tasaro University School of MedIci”?. Sagamlhara
PARTIAL
!‘i’e concluded
levels. suprronlde ‘lpproacl
a"l"n
anlo” level study the pathogenesla
the m~asu,-ement 01 that I” RBC would bc a” useful Involvement of suprronldes of SS.
576
575 jl!i'l;KOXlfl~ UISHLIASE SKIN
I,
LO the
IN NOREIAL HUMAN SKIN .PSOKIASIS.AND
EXPRESSION OF HEAT SHOCK PROTEIN HUMAN SKIN
IlMOl69N IHHtiNOHlSTOCHEMlCAL STUDY.
14KASlll KOCAYASHI.ilII'SLHIKOHATSUMOTO.HhJl~E
72 IN NSF3AL
YAMAJI, TSUTOClU MURAMATSU, HIDEYUKI TADA, MASAC NOBUHIKO KOBAYASHI, TOSHIHIKO SHIRAI AND TAKE0 OJ
1lZUKA and
Compared with other organs, human skin 1s considered to be more iniluenced by environmental 6tressei; such as heat and ultravlolet(UV). To date, there has been few reports of heat shock protelns(HSPs) at human organ level. When orqan cultured normal human skin was heated at 45'C for 30 min. HSP-72 was detected in the nuclei of the epidermis by immunofluorescence methods using anti-HSP-72 monoclonal antibody. iie also intend to report the induction of HSP-72 by other stresses such as uV, heavy metals and chemicals II, orqan cultured skin.
577 HEAT SHOCK PROTEINS FiBROBLASTS
IN CULTURED
AUMAN SKIN
HLDEYUKI TADA, TSUTOMU MURAMATSU, MASAMI YAMAJI. NOBUtlIKO KOBAYASHI, TOSHIHIKO SHlRAI AND TAKE0 OHNISHI* Departments of Dermatology and Biology*, Nara MedIcal University, !Wra ~11 organisms from bacteria to human respond to abrupt changes of high temperature by inducing the synthesis of a number of proteins. These prOteins termed the heat shock prOteins(HSPS). In the present study, we have investigated the HSPs induced in normal human and XP complementation qroup A(XP-A) skin flbroblasts. When the cells wire heated at 45°C for 1 h, HSP-72 was detected in the nucleoli of normal cells, but not in the nucleoli of XP-A cells, by immunofluorescence methods using antl-HSP-72 antibody. Other types of HSF's were also examined by two-dimensional qrl electrophoresis for [35SI-methionine labeled proteins induced by heat treatment.
243
574
DIPEPTI9YL OF x-pro PURIFICATION AMINOPEPTIDASE FROM RAT SKIN HIPOSHI SHUNJI NAKANO, MASAHIR" KUSUHARA, HACilISUKA AND YOICHIRO SASAI University of Dermatoloqy, Kurume Department School of Med1clnc, Kurume A" enzyme with the speclflclty of a X-?rO purlfief+ fron rllpfptlclyl amlnopeptldase was rat Si;lii. 7-iGly-Pro)-4-methylCOumarin~mide was used for deternlnation of (Gly-Pro-YCA) has P pH The enzyme actLvity. rhe enzyme weight r,pt3m1,m of 5.0 to 5.5 and a molecular The enzyme t3y gel flltratio". aholit llO,Ofli? actl\Tlty 1s strongly inhlhited by the serl"e SETI protease InhIbitor DFP and PMSF. NEM a"d functlo" of ?Llave no effect on activity. The of enzyme mlqht hr the degradation this i-ub?tance r.
‘THE ELE;VATED LEVEL OF KED BLOOD CELL SUPEROXIDE ANION IN PATIENTS WITH SYSTEMIC SCLEROSISISSI SI1ICEO KONDOU, KAZUE SUGAYA, AlSUKO SEKI?JE. HlhARU SHIGEO NISHIYPSV1A, KL’NIE -* ETO, HARUE ARAI, Departments of Dermatology and Molecular Biology*, ti~tasaro University School of MedIci”?. Sagamlhara
PARTIAL
!‘i’e concluded
levels. suprronlde ‘lpproacl
a"l"n
anlo” level study the pathogenesla
the m~asu,-ement 01 that I” RBC would bc a” useful Involvement of suprronldes of SS.
576
575 jl!i'l;KOXlfl~ UISHLIASE SKIN
I,
LO the
IN NOREIAL HUMAN SKIN .PSOKIASIS.AND
EXPRESSION OF HEAT SHOCK PROTEIN HUMAN SKIN
IlMOl69N IHHtiNOHlSTOCHEMlCAL STUDY.
14KASlll KOCAYASHI.ilII'SLHIKOHATSUMOTO.HhJl~E
72 IN NSF3AL
YAMAJI, TSUTOClU MURAMATSU, HIDEYUKI TADA, MASAC NOBUHIKO KOBAYASHI, TOSHIHIKO SHIRAI AND TAKE0 OJ
1lZUKA and
Compared with other organs, human skin 1s considered to be more iniluenced by environmental 6tressei; such as heat and ultravlolet(UV). To date, there has been few reports of heat shock protelns(HSPs) at human organ level. When orqan cultured normal human skin was heated at 45'C for 30 min. HSP-72 was detected in the nuclei of the epidermis by immunofluorescence methods using anti-HSP-72 monoclonal antibody. iie also intend to report the induction of HSP-72 by other stresses such as uV, heavy metals and chemicals II, orqan cultured skin.
577 HEAT SHOCK PROTEINS FiBROBLASTS
IN CULTURED
AUMAN SKIN
HLDEYUKI TADA, TSUTOMU MURAMATSU, MASAMI YAMAJI. NOBUtlIKO KOBAYASHI, TOSHIHIKO SHlRAI AND TAKE0 OHNISHI* Departments of Dermatology and Biology*, Nara MedIcal University, !Wra ~11 organisms from bacteria to human respond to abrupt changes of high temperature by inducing the synthesis of a number of proteins. These prOteins termed the heat shock prOteins(HSPS). In the present study, we have investigated the HSPs induced in normal human and XP complementation qroup A(XP-A) skin flbroblasts. When the cells wire heated at 45°C for 1 h, HSP-72 was detected in the nucleoli of normal cells, but not in the nucleoli of XP-A cells, by immunofluorescence methods using antl-HSP-72 antibody. Other types of HSF's were also examined by two-dimensional qrl electrophoresis for [35SI-methionine labeled proteins induced by heat treatment.
243