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High-performance swCNT-FET-based bioelectronic tongue using human taste receptor protein
S152
Abstracts / Journal of Bioscience and Bioengineering 108 (2009) S147–S164
MN-O11 High-performance swCNT-FET-based bioelectronic tongue using human taste receptor protein Hyun Seok Song,1 Tae Hyun Kim,3 Sang Hun Lee,1 Un-Kyung Kim,2 Seunghun Hong,3 and Tai Hyun Park1 School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea 1 College of Natural Sciences, Kyungpook National University, Deagu, Republic of Korea 2 and Department of Physics and Astronomy, Seoul National University, Seoul, Republic of Korea 3 Development of an artificial tongue is important for quality control in the food and beverage industry. However, ‘Electronic tongues’ still have problems in the efficiency in terms of sensitivity and selectivity [1]. On the other hand, the bioelectronic noses utilizing biological olfactory receptor proteins belonging to G protein-coupled receptor (GPCR) family have been reported and showed high performance for odour detection [2]. The chemosensory receptors for bitter, sweet, and umami taste have been identified as GPCR [3]. We applied biological human taste receptor protein to the development of artificial tongue, which is similar strategy to the bioelectronic nose. Here, we report human taste receptor, hT2R38 expressed from Escherichia coli, immobilized on single-walled carbon nanotube (swCNT)-field effect transistor (FET) can detect bitter tastes, like human tongue, with high sensitivity and selectivity. The taster haplotype (PAV) of hT2R38 on swCNT-FET responded to bitterness compounds, phenylthiocarbamide (PTC) and propylthiouracil (PROP), but non-taster haplotype (AVI) did not. This hT2R38-functionalised swCNT-FET bioelectronic tongue showed very similar to performance with human taste system [4].
between the two oligonucleotides leads to a fluorescence-quenched duplex which undergoes adenosine-induced dissociation and accordingly results in an increase of FAM fluorescence. Detection sensitivity of the aptamer-based nanosensor depends on the total number of base paring. As a practical application, we demonstrated that the adenosine nanosensor can be used to quantify adenosine deaminase which is clinically important. It is known that mutations in the adenosine deaminase gene are involved in many diseases. As ADA converts adenosine into inosine that does not affect the hybridization state of the nanosensor, fluorescence emission increases because of fast binding equilibrium between adenosine and the aptamer sequence. The adenosine nanosensor enabled accurate determination of ADA concentration. The higher concentration of ADA gave more rapid decrease in the fluorescent intensity, indicating that ADA concentration can be evaluated by monitoring the fluorescence intensity. Our results illustrate that DNA aptamer can be exploited to design sensor materials, permitting sensitive detection of biochemicals that are difficult to be measured by conventional biochemical assay methods. Acknowledgement: This work was supported by BK21 program from the Korean Ministry of Education and Seoul R&BD Program (NT080612,KU080657). References 1. Mayer, G.: The chemical biology of aptamers, Angewandte Chemie-International Edition, 48, 2672-2689 (2009). 2. Zhao, W., Chiuman, W., Lam, J.C.F., McManus, S.A., Chen, W., Cui, Y., Pelton, R., Brook, M.A., and Li, Y.: DNA aptamer folding on gold nanoparticles: from colloid chemistry to biosensors, J. Amer. Chem. Society, 130, 3610-3618 (2008). doi:10.1016/j.jbiosc.2009.08.408
References 1. Terry, L. A., White, S. F., and Tigwell, L. J.: The application of biosensors to fresh produce and the wider food industry, J. Agric. Food Chem., 53, 1309-1316 (2005). 2. Kim, T. H., Lee, S. H., Lee, J., Song, H. S., Oh, E. H., Park, T. H., and Hong, S.: Singlecarbon–atomic-resolution detection of odorant molecules using a human olfactory receptor-based bioelectronic nose, Adv. Mater., 21, 91-94 (2009). 3. Lindemann, B.: Receptors and transduction in taste, Nature, 413, 219-225 (2001). 4. Kim, U.-k., Jorgenson, E., Coon, H., Leppert, M., Risch, N., and Drayna, D.: Positional cloning of the human quantitative trait locus underlying taste sensitivity to phenylthiocarbamide, Science, 299, 1221-1225 (2003).
MN-O13 Detection of pathogen bacteria using fiber optic sensor with functional lipid vesicle HoSup Jung,1 KeonWoo Kwon,1 and KeeSung Kim1,2
doi:10.1016/j.jbiosc.2009.08.407
School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, Republic of Korea 1 and Institute of Advanced Machinery and Design, Seoul National University, Seoul, Republic of Korea 2
MN-O12
The rapid kit using immune chromatography assay has low sensitivity for detection of pathogen bacteria compared with the ELISA method. Because this infectious disease causes a fatal economy loss to farmers, the new rapid sensor system is required at this field with high sensitivity. Fiber optic sensor, which is used for optical scattering using immunoagglutination phenomena between antibody and antigen, is showing good results for the detection of some pathogenic bacteria with high sensitivity. On the other hand, microfluidic immunosensor could improve immunoassay performance by reducing the consumption of reagents, decreasing analysis time, increasing reliability and sensitivity through automation, and integrating multiple processes in a single device. Thus, this optical microfluidic sensor system is useful in developing novel detection method because the operative principle is to simply detect disease. In this research, we used the functional lipid vesicle coated with latex bead instead of carboxylated latex bead for the immobilization of the antibody. Functional lipid vesicle can preserve the activity of the
FRET-based DNA nanosensor for adenosine deaminase assay Jandi Kim, and Jongshik Shin Yonsei University, Seoul, Republic of Korea Aptamers are nucleic acids that bind specifically to target biomolecules with binding affinity as high as antibodies [1]. The secondary structure of the aptamer undergoes a dramatic change upon binding to a target biomolecule, which renders biosensing applications possible [2]. We constructed aptamer-based nanosensors of which fluorescence signal is responsive to adenosine. The DNA nanosensor consists of two oligonucleotides; a FAM-labeled oligonucleotide carrying an adenosine aptamer sequence and its complementary one labeled with a quencher, BHQ1. Hybridization
Abstracts / Journal of Bioscience and Bioengineering 108 (2009) S147–S164
MN-O11 High-performance swCNT-FET-based bioelectronic tongue using human taste receptor protein Hyun Seok Song,1 Tae Hyun Kim,3 Sang Hun Lee,1 Un-Kyung Kim,2 Seunghun Hong,3 and Tai Hyun Park1 School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea 1 College of Natural Sciences, Kyungpook National University, Deagu, Republic of Korea 2 and Department of Physics and Astronomy, Seoul National University, Seoul, Republic of Korea 3 Development of an artificial tongue is important for quality control in the food and beverage industry. However, ‘Electronic tongues’ still have problems in the efficiency in terms of sensitivity and selectivity [1]. On the other hand, the bioelectronic noses utilizing biological olfactory receptor proteins belonging to G protein-coupled receptor (GPCR) family have been reported and showed high performance for odour detection [2]. The chemosensory receptors for bitter, sweet, and umami taste have been identified as GPCR [3]. We applied biological human taste receptor protein to the development of artificial tongue, which is similar strategy to the bioelectronic nose. Here, we report human taste receptor, hT2R38 expressed from Escherichia coli, immobilized on single-walled carbon nanotube (swCNT)-field effect transistor (FET) can detect bitter tastes, like human tongue, with high sensitivity and selectivity. The taster haplotype (PAV) of hT2R38 on swCNT-FET responded to bitterness compounds, phenylthiocarbamide (PTC) and propylthiouracil (PROP), but non-taster haplotype (AVI) did not. This hT2R38-functionalised swCNT-FET bioelectronic tongue showed very similar to performance with human taste system [4].
between the two oligonucleotides leads to a fluorescence-quenched duplex which undergoes adenosine-induced dissociation and accordingly results in an increase of FAM fluorescence. Detection sensitivity of the aptamer-based nanosensor depends on the total number of base paring. As a practical application, we demonstrated that the adenosine nanosensor can be used to quantify adenosine deaminase which is clinically important. It is known that mutations in the adenosine deaminase gene are involved in many diseases. As ADA converts adenosine into inosine that does not affect the hybridization state of the nanosensor, fluorescence emission increases because of fast binding equilibrium between adenosine and the aptamer sequence. The adenosine nanosensor enabled accurate determination of ADA concentration. The higher concentration of ADA gave more rapid decrease in the fluorescent intensity, indicating that ADA concentration can be evaluated by monitoring the fluorescence intensity. Our results illustrate that DNA aptamer can be exploited to design sensor materials, permitting sensitive detection of biochemicals that are difficult to be measured by conventional biochemical assay methods. Acknowledgement: This work was supported by BK21 program from the Korean Ministry of Education and Seoul R&BD Program (NT080612,KU080657). References 1. Mayer, G.: The chemical biology of aptamers, Angewandte Chemie-International Edition, 48, 2672-2689 (2009). 2. Zhao, W., Chiuman, W., Lam, J.C.F., McManus, S.A., Chen, W., Cui, Y., Pelton, R., Brook, M.A., and Li, Y.: DNA aptamer folding on gold nanoparticles: from colloid chemistry to biosensors, J. Amer. Chem. Society, 130, 3610-3618 (2008). doi:10.1016/j.jbiosc.2009.08.408
References 1. Terry, L. A., White, S. F., and Tigwell, L. J.: The application of biosensors to fresh produce and the wider food industry, J. Agric. Food Chem., 53, 1309-1316 (2005). 2. Kim, T. H., Lee, S. H., Lee, J., Song, H. S., Oh, E. H., Park, T. H., and Hong, S.: Singlecarbon–atomic-resolution detection of odorant molecules using a human olfactory receptor-based bioelectronic nose, Adv. Mater., 21, 91-94 (2009). 3. Lindemann, B.: Receptors and transduction in taste, Nature, 413, 219-225 (2001). 4. Kim, U.-k., Jorgenson, E., Coon, H., Leppert, M., Risch, N., and Drayna, D.: Positional cloning of the human quantitative trait locus underlying taste sensitivity to phenylthiocarbamide, Science, 299, 1221-1225 (2003).
MN-O13 Detection of pathogen bacteria using fiber optic sensor with functional lipid vesicle HoSup Jung,1 KeonWoo Kwon,1 and KeeSung Kim1,2
doi:10.1016/j.jbiosc.2009.08.407
School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, Republic of Korea 1 and Institute of Advanced Machinery and Design, Seoul National University, Seoul, Republic of Korea 2
MN-O12
The rapid kit using immune chromatography assay has low sensitivity for detection of pathogen bacteria compared with the ELISA method. Because this infectious disease causes a fatal economy loss to farmers, the new rapid sensor system is required at this field with high sensitivity. Fiber optic sensor, which is used for optical scattering using immunoagglutination phenomena between antibody and antigen, is showing good results for the detection of some pathogenic bacteria with high sensitivity. On the other hand, microfluidic immunosensor could improve immunoassay performance by reducing the consumption of reagents, decreasing analysis time, increasing reliability and sensitivity through automation, and integrating multiple processes in a single device. Thus, this optical microfluidic sensor system is useful in developing novel detection method because the operative principle is to simply detect disease. In this research, we used the functional lipid vesicle coated with latex bead instead of carboxylated latex bead for the immobilization of the antibody. Functional lipid vesicle can preserve the activity of the
FRET-based DNA nanosensor for adenosine deaminase assay Jandi Kim, and Jongshik Shin Yonsei University, Seoul, Republic of Korea Aptamers are nucleic acids that bind specifically to target biomolecules with binding affinity as high as antibodies [1]. The secondary structure of the aptamer undergoes a dramatic change upon binding to a target biomolecule, which renders biosensing applications possible [2]. We constructed aptamer-based nanosensors of which fluorescence signal is responsive to adenosine. The DNA nanosensor consists of two oligonucleotides; a FAM-labeled oligonucleotide carrying an adenosine aptamer sequence and its complementary one labeled with a quencher, BHQ1. Hybridization