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High selenium diet protects against inflammatory bowel disease and restores mitochondrial colonic tissue function
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EUROPEAN SOCIETY OF PARENTERAL AND ENTERAL NUTRlTION
weeks of gestation were enrolled. Only appropriate for gestational age (GA) singleton infants were included. Se serum concentrations were measured by atomic absorption spectrometry, in blood samples from pxturient mothers and their infants (umbilical blood). Demographic, p&natal and labor data wenz obtained, and their correlations with umbilical Se serum concentrations, maternal Se serum concentrations, and the maternal-umbilical Se difference were examined. Results: UC Se serum concentrations increased as gestation progressed (piO.01). A statistically significant line= relationship was found between UC Se serum concentrations and GA (R=0.341, piO.OOOl), birth weight (R=0.237, p=O.O02), and 5-minute Apgar score (R=0.202, piO.01). Using multiple regression analysis, only GA was significantly associated with UC serum Se concentration (p=O.O12). Maternal Se serum concentration were stable throughout gestation, and maternal-umbilical Se difference was negatively correlated with GA (R= -0.337, piO.0001) and birth weight (R= -0.369, piO.0001). Conclusions: UC Se serum concentration increases and the matemal-umbilical Se difference declines with gestation progression, probably reflecting fetal accretion of Se. With the cul7-entSe supplementation in premature infants, Se concentrations are lower than those measured in 0~1’study. We, therefore, suggest a two-fold increase or more in the recommended Se dosage.
paired adaptation of immune cells to nutritional variations. In this context, carotenoids as immunomodulatoly factors could optimize immune functions in elderly people. This study aims to define, in basal condition and after dietxy cxotenoid depletion and repletion, the proliferation response of lymphocytes by measuring IL-2 and RIL-2s production Method: One hunheed healthy male volunteers aged 20-75 yrs were a carotenoid depleted diet for 3 weeks followed by a 5-week-carotenoid I-epleted regimen (30 mg fi-cxotene + 15 mg lycopene + 9 mg lutein per day). Lymphocyte in vitro productions of both IL-2 and its soluble receptor (RIL-2s) were measured by ELISA methods after PHA stimulation during 48 and 96 h at baseline (TO), after carotenoid depletion (Tl) and repletion (T2). Effects of age and carotenoid treatment wele analysed by two way ANOVA + Fisher test. Results: Whatever the age of the volunteers, carotenoid depletion induced an increase in secretion of both IL-2 (in rig/L: 94f16 (Tl) vs 66f13 (TO) p=O.O44) and RIL-2s (in pmol/L: 382f21 (Tl) vs 333f19 (TO), p=O.O78). After repletion, production of IL-2 (58flO rig/L) and RIL-2s (296f19 pmol/L) retuned to baseline values. Conclusions: Carotenoids anz able to modulate the lymphocyte proliferation response underlining the impact of an optimal carotenoid supply during aging.
126-O. FOOD INTAKE AND MICRONUTRIENT PATIENTS WITH CROHN’S DISEASE
128-O. HIGH SELENIUM DIET PROTECTS AGAINST INFLAMMATORY BOWEL DISEASE AND RESTORES MITOCHONDRIAL COLONIC TISSUE FUNCTION
STATUS IN
.I. Filippi, E. Eyraud, R. Al-Jaouni, S.M. Schneider, X. H butelne Gastroenterology and Clinical Nutrition, Archet University Hospital, Nice, France Rationale: Patients with Crohn’s disease (CD) xe at risk of developing nutritional deficiencies, especially due to restrictive diets. The aim of our study was to assessfood intake as well as the status for vitamins and trace elements in non-selected CD patients in remission. Method: CD outpatients in remission (CDAIs150) filled-out complete food records for three days. The records were then analyzed with the help of a dietitian, and detailed food intake computerized. Fasting blood samples were taken to measure plasma levels of vitamins and trace elements. Resting energy expenditure (REE) was measured by indirect calorimetry. Individual data was companzd to normal values (recommended dietary allowances, and 1.3 x REE for daily energy needs). Results: 55 consecutive patients (22 M, 33 F; 42fllyr; BMI=21.6f4.2) were studied. They had colonic (n=23), ileal (n=16), or ileo-colonic (n=26) CD. 15 patients had undergone surgery, 43 had received steroids and 34 had received immunosuppressive drugs. The mean (*SD) energy intake was higher than calculated values (2243 f 580 vs 1886 f 332 kcal/d, P=O.OOl), but in 10 patients (18%) energy intake did not cover the needs. Lower than recommended intake was recorded for dietary fibre (90% of patients), vitamins A (82%), D (78%), E (82%), Bl (62%), B6 (72%), B9 (82%), B12 (37%), C (85%) and PP (45%), calcium (66%), magnesium (76%), iron (59%) and zinc (94%). Low plasma levels wele measued for vitamins Bl (24%), B6 (25%), B9 (29%), B12 (45%), C (74%), and for zinc (73%). Conclusions: In patients with CD, quantitative needs xe usually covered by food intake. However, micronutrient deficiencies xe frequent and call for a specific screening and treatment. The study of a greater number of patients will allow us to determine disease- and patient-related risk factors for specific deficiencies.
127-O. EFFECTS OF DIETARY CAROTENOID DEPLETION AND REPLETION ON LYMPHOCYTE PROLIFERATION DURING AGING
0. Tirosh R. Reifen Schoolf’Nutritional Sciences, Institute of Biochemistry, Food Science and Nutrition, The Hebrew University of Jerusalem, Rehovot, Israel Rationale: We elucidated the affect of selenium rich diet on the inflammatory status and mitochondrial dysfunction in the colonic tissue following colitis induction. Method: Sprague Dawley rats (150-200g) were supplemented with selenium in the folm of sodium selenite for 21 days. The selenium dose for a single rat was approximately 2 microglg body weight/day. Colitis was induced on day 21. Rat were sacrificed after 24 horns and the colon tissue was immediately removed and washed with ice-cold PBS. Tissue selenium content, MPO activity, respiration (oxygen consumption) and lipid peroxidation were evaluated. Results: Selenium supplementation resulted in significant increase in colon tissue selenium levels. 0.24f0.003, 0.15f0.003, 0.34f0.016 and 0.2f0.004 (mg/kg) in control-groups (with and without selenium supplementation) and colitis-groups (with and without selenium supplementation), respectively. Selenium supplementation suppressed tissue MPO activity in TNBS treated rats (n=S). 0.006f0.00004, 0.007f0.0009, 0.03f0.0004 and 0.19f0.008 (U activity/mg protein) in control-groups (with and without selenium supplementation) and colitis-groups (with and without selenium supplementation), respectively. Selenium protected the mitochon&ia in the colon of TNBS treated animals (n=6) as was evaluated by tissue oxygen consumption 19.9f2.3, 8.9f0.29, 5.3f0.7 and 3.6f0.15 (micromol oxygen/m&g) in control-groups (with and without selenium supplementation) and colitis-groups (with and without selenium supplementation), respectively. Conclusions: high selenium diet higher than the RDA levels in humans as well as in the regular diet of rats (around 0.016 microg/g/day) was able to suppress TNBS induced colitis. We show for the first time that tissue damage in colitis is accompanied by the zuxestof mitochondrial respiration and that selenium diet protected mitochondrial respiration effectively.
129-P. THE ABSORPTION OF IRON BISGLYCINATE (IBC) AND FERRUS CALCIUM CITRATE (ICC) IS EQUAL USING AN IRON ORAL TOLERANCE TEST
R. Minet-Quinard t , M.C. Farges’, S. Wahand’, E. Thivat’ , N. Cxdinault’, E. Rock’, .I. Ribalta3, B. Winklhofer-Roob4, M.P. Vasson’ ‘LB2MN, Pharmacy School, Clermont-Ferrand, ’ lJ3M, INRA, The& France, 3 Unitat de Recerca de Lipids i Arteriosclerosi, Universitat Rovira i Virgili, Reus, Spain, 41nst. of Molec. Biol, Biochem, Microbial, Karl-Franzens University, Graz, Austria
I. Chelmesh’, R.R.E. Eliakim’ ’Gastroenterology, internal medicine D, ’Gastroenterology, Rambam Medical Center; Hai$a, Israel
Rationale: Dysimmunity is frequently associated with aging such as an im-
Rationale: Iron deficiency anemia is a common clinical problem. Oral iron
EUROPEAN SOCIETY OF PARENTERAL AND ENTERAL NUTRlTION
weeks of gestation were enrolled. Only appropriate for gestational age (GA) singleton infants were included. Se serum concentrations were measured by atomic absorption spectrometry, in blood samples from pxturient mothers and their infants (umbilical blood). Demographic, p&natal and labor data wenz obtained, and their correlations with umbilical Se serum concentrations, maternal Se serum concentrations, and the maternal-umbilical Se difference were examined. Results: UC Se serum concentrations increased as gestation progressed (piO.01). A statistically significant line= relationship was found between UC Se serum concentrations and GA (R=0.341, piO.OOOl), birth weight (R=0.237, p=O.O02), and 5-minute Apgar score (R=0.202, piO.01). Using multiple regression analysis, only GA was significantly associated with UC serum Se concentration (p=O.O12). Maternal Se serum concentration were stable throughout gestation, and maternal-umbilical Se difference was negatively correlated with GA (R= -0.337, piO.0001) and birth weight (R= -0.369, piO.0001). Conclusions: UC Se serum concentration increases and the matemal-umbilical Se difference declines with gestation progression, probably reflecting fetal accretion of Se. With the cul7-entSe supplementation in premature infants, Se concentrations are lower than those measured in 0~1’study. We, therefore, suggest a two-fold increase or more in the recommended Se dosage.
paired adaptation of immune cells to nutritional variations. In this context, carotenoids as immunomodulatoly factors could optimize immune functions in elderly people. This study aims to define, in basal condition and after dietxy cxotenoid depletion and repletion, the proliferation response of lymphocytes by measuring IL-2 and RIL-2s production Method: One hunheed healthy male volunteers aged 20-75 yrs were a carotenoid depleted diet for 3 weeks followed by a 5-week-carotenoid I-epleted regimen (30 mg fi-cxotene + 15 mg lycopene + 9 mg lutein per day). Lymphocyte in vitro productions of both IL-2 and its soluble receptor (RIL-2s) were measured by ELISA methods after PHA stimulation during 48 and 96 h at baseline (TO), after carotenoid depletion (Tl) and repletion (T2). Effects of age and carotenoid treatment wele analysed by two way ANOVA + Fisher test. Results: Whatever the age of the volunteers, carotenoid depletion induced an increase in secretion of both IL-2 (in rig/L: 94f16 (Tl) vs 66f13 (TO) p=O.O44) and RIL-2s (in pmol/L: 382f21 (Tl) vs 333f19 (TO), p=O.O78). After repletion, production of IL-2 (58flO rig/L) and RIL-2s (296f19 pmol/L) retuned to baseline values. Conclusions: Carotenoids anz able to modulate the lymphocyte proliferation response underlining the impact of an optimal carotenoid supply during aging.
126-O. FOOD INTAKE AND MICRONUTRIENT PATIENTS WITH CROHN’S DISEASE
128-O. HIGH SELENIUM DIET PROTECTS AGAINST INFLAMMATORY BOWEL DISEASE AND RESTORES MITOCHONDRIAL COLONIC TISSUE FUNCTION
STATUS IN
.I. Filippi, E. Eyraud, R. Al-Jaouni, S.M. Schneider, X. H butelne Gastroenterology and Clinical Nutrition, Archet University Hospital, Nice, France Rationale: Patients with Crohn’s disease (CD) xe at risk of developing nutritional deficiencies, especially due to restrictive diets. The aim of our study was to assessfood intake as well as the status for vitamins and trace elements in non-selected CD patients in remission. Method: CD outpatients in remission (CDAIs150) filled-out complete food records for three days. The records were then analyzed with the help of a dietitian, and detailed food intake computerized. Fasting blood samples were taken to measure plasma levels of vitamins and trace elements. Resting energy expenditure (REE) was measured by indirect calorimetry. Individual data was companzd to normal values (recommended dietary allowances, and 1.3 x REE for daily energy needs). Results: 55 consecutive patients (22 M, 33 F; 42fllyr; BMI=21.6f4.2) were studied. They had colonic (n=23), ileal (n=16), or ileo-colonic (n=26) CD. 15 patients had undergone surgery, 43 had received steroids and 34 had received immunosuppressive drugs. The mean (*SD) energy intake was higher than calculated values (2243 f 580 vs 1886 f 332 kcal/d, P=O.OOl), but in 10 patients (18%) energy intake did not cover the needs. Lower than recommended intake was recorded for dietary fibre (90% of patients), vitamins A (82%), D (78%), E (82%), Bl (62%), B6 (72%), B9 (82%), B12 (37%), C (85%) and PP (45%), calcium (66%), magnesium (76%), iron (59%) and zinc (94%). Low plasma levels wele measued for vitamins Bl (24%), B6 (25%), B9 (29%), B12 (45%), C (74%), and for zinc (73%). Conclusions: In patients with CD, quantitative needs xe usually covered by food intake. However, micronutrient deficiencies xe frequent and call for a specific screening and treatment. The study of a greater number of patients will allow us to determine disease- and patient-related risk factors for specific deficiencies.
127-O. EFFECTS OF DIETARY CAROTENOID DEPLETION AND REPLETION ON LYMPHOCYTE PROLIFERATION DURING AGING
0. Tirosh R. Reifen Schoolf’Nutritional Sciences, Institute of Biochemistry, Food Science and Nutrition, The Hebrew University of Jerusalem, Rehovot, Israel Rationale: We elucidated the affect of selenium rich diet on the inflammatory status and mitochondrial dysfunction in the colonic tissue following colitis induction. Method: Sprague Dawley rats (150-200g) were supplemented with selenium in the folm of sodium selenite for 21 days. The selenium dose for a single rat was approximately 2 microglg body weight/day. Colitis was induced on day 21. Rat were sacrificed after 24 horns and the colon tissue was immediately removed and washed with ice-cold PBS. Tissue selenium content, MPO activity, respiration (oxygen consumption) and lipid peroxidation were evaluated. Results: Selenium supplementation resulted in significant increase in colon tissue selenium levels. 0.24f0.003, 0.15f0.003, 0.34f0.016 and 0.2f0.004 (mg/kg) in control-groups (with and without selenium supplementation) and colitis-groups (with and without selenium supplementation), respectively. Selenium supplementation suppressed tissue MPO activity in TNBS treated rats (n=S). 0.006f0.00004, 0.007f0.0009, 0.03f0.0004 and 0.19f0.008 (U activity/mg protein) in control-groups (with and without selenium supplementation) and colitis-groups (with and without selenium supplementation), respectively. Selenium protected the mitochon&ia in the colon of TNBS treated animals (n=6) as was evaluated by tissue oxygen consumption 19.9f2.3, 8.9f0.29, 5.3f0.7 and 3.6f0.15 (micromol oxygen/m&g) in control-groups (with and without selenium supplementation) and colitis-groups (with and without selenium supplementation), respectively. Conclusions: high selenium diet higher than the RDA levels in humans as well as in the regular diet of rats (around 0.016 microg/g/day) was able to suppress TNBS induced colitis. We show for the first time that tissue damage in colitis is accompanied by the zuxestof mitochondrial respiration and that selenium diet protected mitochondrial respiration effectively.
129-P. THE ABSORPTION OF IRON BISGLYCINATE (IBC) AND FERRUS CALCIUM CITRATE (ICC) IS EQUAL USING AN IRON ORAL TOLERANCE TEST
R. Minet-Quinard t , M.C. Farges’, S. Wahand’, E. Thivat’ , N. Cxdinault’, E. Rock’, .I. Ribalta3, B. Winklhofer-Roob4, M.P. Vasson’ ‘LB2MN, Pharmacy School, Clermont-Ferrand, ’ lJ3M, INRA, The& France, 3 Unitat de Recerca de Lipids i Arteriosclerosi, Universitat Rovira i Virgili, Reus, Spain, 41nst. of Molec. Biol, Biochem, Microbial, Karl-Franzens University, Graz, Austria
I. Chelmesh’, R.R.E. Eliakim’ ’Gastroenterology, internal medicine D, ’Gastroenterology, Rambam Medical Center; Hai$a, Israel
Rationale: Dysimmunity is frequently associated with aging such as an im-
Rationale: Iron deficiency anemia is a common clinical problem. Oral iron