Histopathologic and prognostic correlations regarding human papillomavirus (HPV) infection in penile squamous cell carcinomas (SCC) considering the novel 2016 WHO classification

32nd Annual EAU Congress, 24-28 March 2017, London, United Kingdom

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Histopathologic and prognostic correlations regarding human papillomavirus (HPV) infection in penile squamous cell carcinomas (SCC) considering the novel 2016 WHO classification Eur Urol Suppl 2017; 16(3);e1242

Hölters S.1, Khalmurzaev O.2, Ueberdiek S.1, Loertzer P.1, Pfuhl T.3, Pryalukhin A.4, Hartmann A.5, Janssen M.1, Loertzer H.6, Wunderlich H.7, Hauschild E.8, Bohle R.M.4, Smola S.3, Stöckle M.1, Matveev V.2, Junker K.1 1

Saarland University, Dept. of Urology and Paediatric Urology, Homburg, Germany, 2N.N. Blokhin Cancer Research Center, Dept. of Urology, Moscow, Russia, 3Saarland University, Institute of Virology, Homburg, Germany, 4Saarland University, Institute of Pathology, Homburg, Germany, 5Erlangen University, Institute of Pathology, Erlangen, Germany, 6Westpfalz-Klinikum GmbH, Dept. of Urology, Kaiserslautern, Germany, 7St Georg Klinikum, Dept. of Urology and Paediatric Urology, Blankenhein, Germany, 8Helios Clinics, Dept. of Urology, Blankenhein, Germany INTRODUCTION & OBJECTIVES: Squamous cell carcinomas of the penis represent the majority of malignant penile tumours. Metastasis is the most important prognostic factor, but less is known about tumourigenesis and progression. About 50% of all SCCs are associated with human papillomaviruses (HPV). Therefore, the WHO published a novel HPV-based classification of penile tumours. The aim of this international multicentre study is the evaluation of histopathologic and prognostic parameters in correlation to HPV in penile squamous cell carcinomas (SCC). MATERIAL & METHODS: We collected data and tumor samples from 172 patients from Russia and Germany. The tumours have been classified considering the new 2016 WHO classification. DNA was isolated from FFPE tissues and the HPV genotyping was performed by qPCR and sequencing. Clinical and histopathological data were correlated to HPV. RESULTS: HPV was detected in 51 % of available tumour samples. HPV 16 represented the most frequent subtype (87%). We observed a clear distribution of HPV in the respective novel subtypes. The HPV‑related subtypes Clear cell carcinoma (100%), Papillary-basaliod (100%), Warty-basaliod (100%), Basaliod (88%) and Warty (67%) were predominantly high risk HPV positive. In addition, the non-HPVrelated subtype Squamous cell carcinoma (usual type) exhibited high risk HPV in 23%. HPV infection varied with age: <50 years (57%), 51-70 years (40%), and >70 years (46%). In 44% of the N- and in 62% of the N+ patients as well as in 48% of patients with pT1-2 and in 28% with pT3-4 tumors high risk HPV occurred. Exclusively high risk HPV 16 and 18 was found in lymph node metastases and the HPV type of metastasis correlated with the HPV type of the corresponding primary tumor. A tendency of higher tumour specific survival rate was observed for patients with high risk HPV positive tumours. CONCLUSIONS: Our results indicate a distinct role of high risk HPV in penile SCC for development of specific histological subtypes, prognosis and tumourigenesis. To validate these results, we continuously expand our patient population and starting molecular analysis to explore the processes of tumourigenesis and metastasis dependent on HPV.

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