HLA-DQA1 and DQB1 alleles and haploypes in Latvians

54

Abstracts

P281

P282

HLA FIVE·LOCUS HAPLOTYPES IN FINNS

HLA-DQA I AND DQSI ALLELESAND HAPLOTYPES IN LATVIANS SochnevA, DenisovaA, Kovalchuk L, Priedite V, SanjeeviCB Medical Academy of Latvia, Riga, Latvia and Karolinska Institute, Stockholm, Sweden. The population in Latvia is distributed into 4 regions: Kurzeme, Vidzeme, Zemgale and Latgale. The aim of the study was to analyse the distributionof the HLA-DQAI and -DQBI alleles and DQ haplotypesin the Latvian population comprising all the four regions. HLA genotyping for -DQAI and -DQSI was done by PCR-SSOin 73 healthy individuals from 3 of the 4 regions: Kurzeme, Vidzeme, Zemgale. The allele and haplotype frequenciesof them (> 10%) are:

Silvennoinen-Kassinen Sylvi, Ikaheimo Irma, Tiilikainen Anja Dept. of Medical Microbiology, Univ. of Oulu, Oulu, Finland Five-locus HLA haplotypes of Finns are described. 364 haplotypes were obtained from random families. A comparison of "common" haplotypes with frequencies ~ 0.8 % in Finns V8. other populations yields following resemblances: The Caucasoid ancestral haplotype A3,Cw4,B35,DR1,DQ1 (the most frequent of Finns, 7.7 %) emerges in Italians and Italians of Celtic origin. Also A2.Cw5,B44.DR5,DQ3 (0.8 % in Finns) is "common" in these populations. A1,Cw7,B8,DR3,DQ2 (4.7 %) is the most frequent in Caucasoids. A3,Cw7,B7,DR2,DQ1 (3.6 %) and A2,Cw7,B7, DR2,DQ1 (2.5 %) are "common" in several Caucasoid populations, theIatter also in Jamaican blacks. A2,Cw6,B13,DR7,DQ2 (1.1 %) emerges in Caucasoids in USA and A9,Cw4,B35,DR1,DQl (0.8%) inMongoloids. A2,Cw3,B62,DR4,DQ3 (3.0 %), A2,Cw2,B27,DRB,DQ4 (1.7 %), A2,Cw3,B62,DR6,DQl (1.4 %) and A2,Cwl,B27,DR4,DQ3 (1.4 %) were also "common" in Finns. These findings are in agreement with the postulation about the migration routes of ancestral Finns from southern Scandinavia and Germany.

HLA IN THE ETHNIC GROUPS OF LITHUANIA A.

s e k s t.e n e ne, s t anev ic ien e

Vilnius

V.

and

Vilnius,

Lithuania

HLA ant igens frequency has been studied In 639 Lithuanians, incruc ma 204 z ema icrar, 196 Eastern AuKStaIClal, 136 Southern Auk s t.a rc i ar and 103 Western

AukStaitlal.

HLA-A ,

B,

C,

DR

antigens

were

tested by the standard Iymphocy t ot ox i c assays. The most frequent antigens In Lithuanians are A2-54.41., A3-28.4%, A9-20.2%, B7-32.2%, B12-19.3%, B27-16.1%, DR2-40.0%, DR5-26.4%, DR3-23.7%, The most frequent HLA haplotypes are A3-B7, A2-B13 and Al-B8 (~'l--29.5; 18.28; 167.5 accordingly). The Significant differences

In

antigen

dts t r-t out.i on

were

found

between z emarc i ai and Auks t.arcra i groups. The Al and 68 antigens frequency in Southern AukStaiCiai (28.7% and 21.2'-) were higher than in zemarcrar (13.2% and 7.8%). DRI antigen frequency i n z ema icrat (31.9%) IS higher than in Western Auk s t a rc i a r (12.1"), while DR7 ant.reen frequency in zemarcrai (15.5%) is lower than i n Eastern AuKStalC1aI (30.5%). These data support the role of HLA polymorphism as mar-ker-

of

genetic

distance

between

e t hn i c

groups.

P285

DETERMINATION OF '!HE ALLELES OF IlIA I CLI\SS IN GEORGIAN, KURDISH AND CZEOl POPULATIONS BY ARMS-PCR MEmlOD

Bendukidze Nina, Meschishvili Elena, IVaSkova Eva, Cerna Marie, Churadze Tamaz, Guladze Ramaz, Shalamberidze Ketevan Institute for Clinical and Experimental Medicine, Prague, Czech Republic; Institute of Pediatrics, '!bilisi, Georgia

we

DQhaplotype

n=l46

*0501 *0602 *0201 *0301

A*0101-S*0501 A*0102-S*0602 A*0201-S*0201 A*05O[-S*mO[ A*mOl-B*mOl

18(12%) 2[ (14%) 15(10%) 15(10%) 20(14%)

18 (12%) 21 (14%) 27 (18%) 38 (26%)

The likagedisequilibrium betweenDQAI and DQSI in Latvians was similar to that seen in SwedishCaucasians.The distributionof A*0[02-B*0602 did not differ between the regions. A*010I-B*0501 (8%) and A*0301-S*0301 (8%) was lower in frequency in Kurzeme, A*0201-B*0201 lower in Zemgale(7%) when comparedto other 2 regions.

THE STUDY OF POPULATION STRUCTURE BASED ON THE ANALYSIS OF HLA-DRB1, DRB3, DRB4, DRBS, DQAI, DQBI POLYMORPHlSMS (AN EXAMPLE OF THE ISLAND OF HVAR, CROATIA)

La ps y t e, R. PIKelyte, R. Hallekaite.

University,

DQSl n=[46

P284

P283 N.

DQAI n=l46 *0101 22 (15%) *010232(22%) *0201 20 (14%) *0301 27 (18%) *05O[ 29(20%)

have studied the !!LA-class I antigen distribution in unrelated healthy individuals fran three different ethnic groups l:rt means of the DNA typing method base:'! on the principle of the amplification refractory mutation systan (ARMS-PCR) using the reagens and the manual of the 12th IIIW. we have collected lCO DNA samples fran Georgians, 30 DNA samples fran Kurds living in the capital of Georgia Tbilisi and 100 DNA samples and frozen lY!1V?hocytes fran ~zechs. These populations have the different genetic drift and historical origin. '!be most part of the typing was performed in Tissue Antigen Laboratory ICRF London, heade:'! by Julia Bodmer. !!LA-A" 02, E'35 and o.~04 are the most characteristic in Georgians. In Czechs, there also A'02 is the most frequent antigen (55,5%). Frequency of A'03, E"'51/52 is the highest in Kurds (32,1% and 34,6%). A'02 and A"'ll are the second most frequent alleles (both 21,4%). Within the C locus, this group has high frequencies of 0."04 and 0."15_ we hope that high resolution will give us the possibility to find considerable variations within the llIA-class I systan in Georgians, Kurds and Czechs.

KastelanAndrija', GrubicZorana', Zonec Renata', Kerhin Vesna', Kastelan Darke', Suman Ana', MartinovicIrena', Bakran Maja', Janiciievic Branka', Jovanovic Veljko', SmolejNina', Rudan Pavao' 'National Referral Organ Transplantationand TissueTyping Centre, Zagreb. Croatia "Institutefor Anthropological Research- Zagreb, Zagreb, Croatia

Allele frequencies at lILA-DRBI, DRB3, DRB4, DQAI, DQBI loci were determined in six rural populationsof the island of Hvar, Croatia, using PCR methodology and non-radioactive oligonucleotide hybridisation. Allele frequencies,measures of genetic kinship(R matrix) and genetic distances (E') were computed. The studied rural populations revealed isolation and clusterisation which coincides with the known ethnohistorical and sociocultural data. The five studied groups formed two clusters (East-West) to which the sixth group (with highest R;, of 0.04036) joined later, thus indicating possible impact of historical immigrations from the mainland. Further on, genetic and geographic distances, as well as migrational kinship were utilised for quadratic assignment procedures indicating high statistical significance for both positive(genetic vs. geographic distances, r,{l.6787) and negative (genetic distance vs. migrational kinship, r~-O. 6109; geographic distancesvs. migrationalkinship, r=-0.5357)correlations. The possibilities and relevance of this kind of anthropological population structure analysis based on lILA polymorphisms is stressed.

P286

HLA-DRBI, DQAl AND DQBI DNA POLYHORPHISHS IN HEALTHY HUNGARIAN POPULATION

E . Varga, G Samu, E. Endreffy, 1. Petri c:..5~~ Blood Transfusion' Centre of Me&ical iJniversity, Szeged. Hungary The polymorphism of HLA class II genes (ORB, OQA and DQB) was investigated in a population of 120 unrelated healthy Hungarians using polymerase chain reaction ,PCR) and peR amplification followed by nonradiocative oligonucleotide hybridization. ihese genes are strongly associated with each other and they form characteristic haplotypes in various ethnic groups. Allele and hap Lot yp e f r equ euc e s , as well as ORB1-DQB1 linkage disequlibria, were calculated. 17 ORB1, 8 OQA1 and 13 DQBl alleles were found in the tested population. ORB1*11011104 and 0401-0412 a rp the most frequent all~l~s at the DRBI locus. The OQAl and DQBl loci are less polymorphic. DQA1*0102 allele predomlnate.s at the DQAl locus, while OQB1*0301 IS hIghly predominant at t.he DQB1 loous The most frequent ORB1-DQ81 haplotypic ~omblnatlons are DRB1*1101-DQ81*0301 and ORB1*0301-DQB1*0201. A1J

i n f o rmat i on s may be f o r HLA and d j s e a s e

these

~~~d t~·~t.1J r e

helpful in i at ion

3,SSOC