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Associations of HLA-DRB1, DQA1 and DQB1 alleles and frequencies of DPB1 alleles in Punjabis: A population from North India
58
Abstracts
P306
P305
HLA CLASS II HAPLOTYPE IN A MADAGASCAN POPULAnON
HLA A, B, DR FREQUENCIES IN REUNION ISLAND
MigotFlorence, Fajardy Isabelle, Deloron Paul,Krisbnamoorthy Rajagopal,
Ricard Corinne and Henni Tawfiq CentreHospllalierDepartemental Bellepierre, 97405 Saint-Denis Cedex
Daeze Pierre-Marie INSERMU13, Paris, FIllDCe
Reunion Island, one of the four french overseas departments, lies in the Indian Ocean, near Madagascar. Uninhabited until the seventeenth century, the island was successively peopled by european settlers, slaves from Madagascar and Africa, indentured laborers from the South of India, shopkeepers from the North of India and the South of China, and civil servants from France. As they all live here in a remarkable harmony, cross breeding level is high, so it is quite impossible to assign a precise phenotype iiS negroid, caucasian or oriental to an individual. HLA typing is performed in our laboratory, mainly for potential bone marrow donors, potential kidney receivers and donors, by using the Iymphocytotoxicity technique. Succint analysis of HLA antigen frequencies on 2167 samples, shows a high frequency of the antigens A2, A9, A11, A1, 812, 817, 835, 85, DRS, DR2, DR6, DR7 and also a high rate of untypable alleles (i.e. «blanks»). The heterogeneity of our recruitment of patients can lead to the introduction of a bias and therefore further studies will be needed to assess our results: increasing of the size of our voluntary donors registry and studies of «blanks ».
P307 DISTRIBUTION
P308
OF HLA ANTIGENS AMONG RAJBANSHIS
Tapas Chaudhuri,
Tarun
Sen,
Sreejata De
Sarkar,
tl.anas Dut ta.
Cellular Immunology Lab , , Deptt. of Zoology, University of No r t h Bengal, Siliguri, INDIA The North Eastern region of India situated in one of the greatest migratioin routes of mankind, had been the habitat of various racial strains like Mongoloids, Aryans and Tibeto-Burman. The region is said to have inhibited mostly by nonAryan tribes having r£ongoloid t rai t 5 & features who were called Rajbanshi, Koch & Polia. Our interest in the present study is to investigate the frequency and distribution of HLA antigens among the Rajbanshi population as published information is yet to be available. Antigens A2, All, B5, B35 and Cw4 have been found with higher frequency whereas the antigens B14 and Clil6 are c omp I e t ely absent. The work is still going on but so far it has been observed that the haplotype AII-B5 occurs with the highest incidence. detail.
The
P309 H.,Mo>,N
Suraksha Aqarwal.
f25fl1
will
be
discussed
in
~clical
Raj Kishore, Suhasini Elhatnagar, 5.5. Genetics,
SGPGIMS, Lucknow-
226014,
India, with diverse but endogamous caste ~rouDS is an ideal testing ground for studies on selective advantage of the HLA polymorphism. Present study was conducted on 512 unrelated Hindu individuals and two endogarmus caste
E~~~~ow,na~liheB1Sa{Jit,,:a~f U'tdtarQ1~~~d~~~lei';, I~~l~~ng HEA
antigen \'\ere determined by serology. Distribution of these antigens at A,B C & DR loci and signIficant haolotypes shQlloling posi t ive linkage disequl l i br-Ltm are described and camared to North and South Indian populat ions reported by other workers. The stud¥ shows a higher frequency of HLA A3, A25, A26 and 1J19 ant i qens and corper-at tve ly lower frequency of A2B, B35, an, and 0'15 antigens in the population of U.P. Haplotype fr'1C]uencles indicate populat ion of Uttar Pradesh (Lucknow) to be closer to Delhi population (North Indians) than to South Indians. Bhargavas and Chaturvedies differ si~nificantlY
~~I~~(? o~:; h"a~eA~is~2g;'e~3~~:?;:e'd~3i~h ~o~lh~~df~~
Hindus.
feature
discussed.
ASSOCIATIONS OF HLA-DRBI, DQAI AND DQBI ALLELES AND FREQUENCIES OF DPBI ALLELES IN PUNJABIS: A POPULATION FROM NORTH INDIA Rajni Rani and Rama Mukherjee National Institute of Immunology, New Delhi-II 0067, India Inspite of strong linkage disequilibrium between HLA class-Il alleles, many haplotypes appear to have developed during evolution by recombination. India has been considered the melting pot of different races, so, to study the racial admixture in Punjabis of North India, alleles of HLA-DRBI, DQAl, DQBI and DPBI were studied using PCR-oligotyping. Haplotypes were constructed using two-loci associations for DQAI-DQBl, DRBI-DQBI and DRBI-DQAI using a computer program. DPBI alleles did not show any linkage disequilibrium with alleles of DRBI, DQAl or DQBI, the latter, however, were in positive linkage disequilibrium with each other. A non caucasian DR2 haplotype DRBI *1501, DQAI*0103, DQBI*06Ol seems to be characteristic of Indian populations. Besides the frequent haplotypes, some rare haplotypes were observed in this population. DPBI *0401 seems to be the most frequent DPBI allele found in this population. Frequencies of other DPBI alleles will be presented.
P310 HLA-DRBI ALLELICPOLYMORPIDSMIN ASIANINDIANS.
PlYlLATICJIl OISlRIEllTICJIl OF LBJ
Agra~l,
of
results
We have studied the DRBI, DQAI, DQBI and DPBI genotypes of Madagascansby the PCR-RFLP method, in order to obtain genetic descriptive information on this population group that has so far not been studied according to the data compiled at the 11th International HistocompatibilityWorkshop. Our study was conducted among lIS unrelated and 50 related subjects from the Merina ethnic group, living in Manarintsoa and Ankazobe, two rural communities of the highlands of Madagascar. Here, we report particular associations between DRBI and DQ loci confirmed by subsequent family studies. At the DRBI locus, the DRS generic type was present in very high frequency (0,46) and included the most prevalent allele DRBI *1202 (0,29). The most frequent alleles at the other loci studied were DQAI *0601 (0,30), DQBI*0301 (0,37) and DPBI*0101 (0,20). Additional HLA typing of relatives revealed the presence of the DRBI*1202-DQAI*06OI-DQBl*0301 haplotype which has so far been described only in population groups of South East Asia and in particular among Javanese. The presence of this particular haplotype confirms the strong impact of South East Asians especially Javanese in the peopling of Madagascar. Moreover the presence in our population of a specific black haplotype DRBI*0701-DQA1*0301-DQBI*0201 is not surprising given the known East african migration to Madagascar. 6
Certain unique naot otvoes as a char-ac ter-t s t ie of EJ1argav8s or O1aturvedies have also been
R Rajalingarnand NK Mehra. All India Institute of MedicalSciences, New Delhi, India. Alleles in DRBlloci have been studied by PCR-SSOP method in 154 healthy individuals from North India and 40 Kashmir Brahmins. The North Indians represent a homogeneous population from the states of Punjab, Haryana, Uttar Pradesh and Delhi while Kashmir Brahmins are a more conserved population from the Kashmir valley of Northern India. The results are depicted in the following table. DRBI North Indians KashmirBrahmins alleles (n-154) (n=40) DRBI*OI 10(6.5%) 1(2.5%) DRBI *15,16 62(40.3%) 9 (22.5%) DRB1*03 13(8.4%) 6 (15%) DRBI*04 21(13.6%) 9 (22.5%) DRBI'II 32(20.8%) 8 (20%) DRBI*12 8 (5.2%) 2 (5%) DRBl*13 12(7.8%) 4 (10%) DRBI*14 21(13.6%) 8 (20%) 19(47.5%) DRBI*07 39(25.3%) DRBI*08 3 (1.9%) 0 DRBl*09 3 (1.9%) 0 DRBI*10 24(15.6%) 3 (7.5%) The data shows uniquenessof the Asian Indian populationsas regards their HLA class II profile as comparedto other populations.
Abstracts
P306
P305
HLA CLASS II HAPLOTYPE IN A MADAGASCAN POPULAnON
HLA A, B, DR FREQUENCIES IN REUNION ISLAND
MigotFlorence, Fajardy Isabelle, Deloron Paul,Krisbnamoorthy Rajagopal,
Ricard Corinne and Henni Tawfiq CentreHospllalierDepartemental Bellepierre, 97405 Saint-Denis Cedex
Daeze Pierre-Marie INSERMU13, Paris, FIllDCe
Reunion Island, one of the four french overseas departments, lies in the Indian Ocean, near Madagascar. Uninhabited until the seventeenth century, the island was successively peopled by european settlers, slaves from Madagascar and Africa, indentured laborers from the South of India, shopkeepers from the North of India and the South of China, and civil servants from France. As they all live here in a remarkable harmony, cross breeding level is high, so it is quite impossible to assign a precise phenotype iiS negroid, caucasian or oriental to an individual. HLA typing is performed in our laboratory, mainly for potential bone marrow donors, potential kidney receivers and donors, by using the Iymphocytotoxicity technique. Succint analysis of HLA antigen frequencies on 2167 samples, shows a high frequency of the antigens A2, A9, A11, A1, 812, 817, 835, 85, DRS, DR2, DR6, DR7 and also a high rate of untypable alleles (i.e. «blanks»). The heterogeneity of our recruitment of patients can lead to the introduction of a bias and therefore further studies will be needed to assess our results: increasing of the size of our voluntary donors registry and studies of «blanks ».
P307 DISTRIBUTION
P308
OF HLA ANTIGENS AMONG RAJBANSHIS
Tapas Chaudhuri,
Tarun
Sen,
Sreejata De
Sarkar,
tl.anas Dut ta.
Cellular Immunology Lab , , Deptt. of Zoology, University of No r t h Bengal, Siliguri, INDIA The North Eastern region of India situated in one of the greatest migratioin routes of mankind, had been the habitat of various racial strains like Mongoloids, Aryans and Tibeto-Burman. The region is said to have inhibited mostly by nonAryan tribes having r£ongoloid t rai t 5 & features who were called Rajbanshi, Koch & Polia. Our interest in the present study is to investigate the frequency and distribution of HLA antigens among the Rajbanshi population as published information is yet to be available. Antigens A2, All, B5, B35 and Cw4 have been found with higher frequency whereas the antigens B14 and Clil6 are c omp I e t ely absent. The work is still going on but so far it has been observed that the haplotype AII-B5 occurs with the highest incidence. detail.
The
P309 H.,Mo>,N
Suraksha Aqarwal.
f25fl1
will
be
discussed
in
~clical
Raj Kishore, Suhasini Elhatnagar, 5.5. Genetics,
SGPGIMS, Lucknow-
226014,
India, with diverse but endogamous caste ~rouDS is an ideal testing ground for studies on selective advantage of the HLA polymorphism. Present study was conducted on 512 unrelated Hindu individuals and two endogarmus caste
E~~~~ow,na~liheB1Sa{Jit,,:a~f U'tdtarQ1~~~d~~~lei';, I~~l~~ng HEA
antigen \'\ere determined by serology. Distribution of these antigens at A,B C & DR loci and signIficant haolotypes shQlloling posi t ive linkage disequl l i br-Ltm are described and camared to North and South Indian populat ions reported by other workers. The stud¥ shows a higher frequency of HLA A3, A25, A26 and 1J19 ant i qens and corper-at tve ly lower frequency of A2B, B35, an, and 0'15 antigens in the population of U.P. Haplotype fr'1C]uencles indicate populat ion of Uttar Pradesh (Lucknow) to be closer to Delhi population (North Indians) than to South Indians. Bhargavas and Chaturvedies differ si~nificantlY
~~I~~(? o~:; h"a~eA~is~2g;'e~3~~:?;:e'd~3i~h ~o~lh~~df~~
Hindus.
feature
discussed.
ASSOCIATIONS OF HLA-DRBI, DQAI AND DQBI ALLELES AND FREQUENCIES OF DPBI ALLELES IN PUNJABIS: A POPULATION FROM NORTH INDIA Rajni Rani and Rama Mukherjee National Institute of Immunology, New Delhi-II 0067, India Inspite of strong linkage disequilibrium between HLA class-Il alleles, many haplotypes appear to have developed during evolution by recombination. India has been considered the melting pot of different races, so, to study the racial admixture in Punjabis of North India, alleles of HLA-DRBI, DQAl, DQBI and DPBI were studied using PCR-oligotyping. Haplotypes were constructed using two-loci associations for DQAI-DQBl, DRBI-DQBI and DRBI-DQAI using a computer program. DPBI alleles did not show any linkage disequilibrium with alleles of DRBI, DQAl or DQBI, the latter, however, were in positive linkage disequilibrium with each other. A non caucasian DR2 haplotype DRBI *1501, DQAI*0103, DQBI*06Ol seems to be characteristic of Indian populations. Besides the frequent haplotypes, some rare haplotypes were observed in this population. DPBI *0401 seems to be the most frequent DPBI allele found in this population. Frequencies of other DPBI alleles will be presented.
P310 HLA-DRBI ALLELICPOLYMORPIDSMIN ASIANINDIANS.
PlYlLATICJIl OISlRIEllTICJIl OF LBJ
Agra~l,
of
results
We have studied the DRBI, DQAI, DQBI and DPBI genotypes of Madagascansby the PCR-RFLP method, in order to obtain genetic descriptive information on this population group that has so far not been studied according to the data compiled at the 11th International HistocompatibilityWorkshop. Our study was conducted among lIS unrelated and 50 related subjects from the Merina ethnic group, living in Manarintsoa and Ankazobe, two rural communities of the highlands of Madagascar. Here, we report particular associations between DRBI and DQ loci confirmed by subsequent family studies. At the DRBI locus, the DRS generic type was present in very high frequency (0,46) and included the most prevalent allele DRBI *1202 (0,29). The most frequent alleles at the other loci studied were DQAI *0601 (0,30), DQBI*0301 (0,37) and DPBI*0101 (0,20). Additional HLA typing of relatives revealed the presence of the DRBI*1202-DQAI*06OI-DQBl*0301 haplotype which has so far been described only in population groups of South East Asia and in particular among Javanese. The presence of this particular haplotype confirms the strong impact of South East Asians especially Javanese in the peopling of Madagascar. Moreover the presence in our population of a specific black haplotype DRBI*0701-DQA1*0301-DQBI*0201 is not surprising given the known East african migration to Madagascar. 6
Certain unique naot otvoes as a char-ac ter-t s t ie of EJ1argav8s or O1aturvedies have also been
R Rajalingarnand NK Mehra. All India Institute of MedicalSciences, New Delhi, India. Alleles in DRBlloci have been studied by PCR-SSOP method in 154 healthy individuals from North India and 40 Kashmir Brahmins. The North Indians represent a homogeneous population from the states of Punjab, Haryana, Uttar Pradesh and Delhi while Kashmir Brahmins are a more conserved population from the Kashmir valley of Northern India. The results are depicted in the following table. DRBI North Indians KashmirBrahmins alleles (n-154) (n=40) DRBI*OI 10(6.5%) 1(2.5%) DRBI *15,16 62(40.3%) 9 (22.5%) DRB1*03 13(8.4%) 6 (15%) DRBI*04 21(13.6%) 9 (22.5%) DRBI'II 32(20.8%) 8 (20%) DRBI*12 8 (5.2%) 2 (5%) DRBl*13 12(7.8%) 4 (10%) DRBI*14 21(13.6%) 8 (20%) 19(47.5%) DRBI*07 39(25.3%) DRBI*08 3 (1.9%) 0 DRBl*09 3 (1.9%) 0 DRBI*10 24(15.6%) 3 (7.5%) The data shows uniquenessof the Asian Indian populationsas regards their HLA class II profile as comparedto other populations.