- Email: [email protected]
HYPOLIPIDEMIC EFFECT OF ARONIA MELANOCARPA FRUIT JUICE IN STREPTOZOTOCIN-INDUCED DIABETIC RATS
Poster Sessions PO41 Screening for CV risk – circulating markers
172 PO40-629
HYPOLIPIDEMIC EFFECT OF ARONIA MELANOCARPA FRUIT JUICE IN STREPTOZOTOCIN-INDUCED DIABETIC RATS
PO40-631
MUTATIONS IN THE LDLR AND APOB IN SUBJECTS WITH THE CLINICAL DIAGNOSIS OF FAMILIAL COMBINED HYPERLIPERLIPIDEMIA
S.V. Valcheva-Kuzmanova 1 , K.A. Kuzmanov 2 , S. Tancheva 3 , A. Belcheva 1 . 1 Department of Preclinical and Clinical Pharmacology and Biochemistry, Medical University, Varna, Bulgaria; 2 Vivarium, Medical University, Varna, Bulgaria; 3 Department of Clinical Laboratory, Medical University, Varna, Bulgaria
F. Civeira 1 , E. Jarauta 1 , A. Cenarro 1 , A.L. Garcia-Otin 1 , E. Ros 2 , D. Tejedor 3 , M. Pocovi 4 . 1 Hospital Universitario Miguel Servet, Zaragoza, Spain; 2 Hospital Clinic, Barcelona, Spain; 3 Progenika Biopharma S.A. Derio, Spain; 4 Departamento de Bioquimica, Biologia Molecular y Celular, Universidad de Zaragoza, Zaragoza, Spain
The increased risk for developing premature atherosclerosis in diabetes is due to increase in plasma triglycerides (TG) and low-density lipoproteins (LDL) and decrease in high-density lipoproteins (HDL). Aronia melanocarpa fruit juice (AMFJ) is rich in phenolic antioxidants, especially flavonoids from the anthocyanin subclass. The aim of the present study was to investigate the influence of AMFJ on plasma lipids in diabetic rats. Diabetes was induced by an intraperitoneal injection of streptozotocin (50 mg/kg). AMFJ was applied by gavage at doses of 10 and 20 ml/kg for six weeks to normal and diabetic rats. Streptozotocin application resulted in a significant elevation of plasma glucose by 141% in comparison with normal rats. This effect was accompanied by a significant elevation of plasma TG by 64%, statistically insignificant elevations of total cholesterol and LDL-cholesterol, and a reduction of HDL-cholesterol. Applied to normal rats, AMFJ did not influence plasma glucose and lipid levels. Applied to diabetic rats, the two AMFJ doses reduced significantly plasma TG by 35% and 39%, respectively, to levels that did not significantly differ from those of normal rats and counteracted the influence of streptozotocin on total cholesterol, LDL-cholesterol and HDL-cholesterol. This effect of AMFJ (10 and 20 ml/kg) on plasma lipids was probably due to improvement of the diabetic condition estimated by the significant reduction of plasma glucose by 44% and 42%, respectively. In conclusion, AMFJ reduced the abnormalities in blood lipids in diabetic rats and might be useful in prevention of diabetes-associated atherosclerosis.
Background and aims: To describe the frequency of LDLR and APOB mutations responsible of familial hyperholesteromia (FH) in a group of unrelated subjects with the diagnosis of familial combined hyperlipidemia (FCH) due to presence of mixed hyperlipidemia, familial presentation and absence of tendon xanthomas. Methods: 142 unrelated patients, aged >18 years, with the diagnosis of FCH were selected. The screening of mutations in the LDLR and APOB genes was performed with the Lipochip® , a microarray that includes the diagnosis of 203causative mutations in the LDLR gene and 4 mutations in APOB. Results: 92 men and 50 women were included. In 28 of them (19.7%) a functional mutation in the LDLR was found. Patients with LDLR mutation (LDLR+) had higher total cholesterol, Non HDL cholesterol and apo B concentrations than patients without mutation (LDLR-). However, triglycerides concentration was almost significantly higher in LDLR- than in LDL+ subjects (p=0.063). The frequency of mutation in women was 28% (14 out of 50), higher than in men (15.6%), although this difference did not reach statistical significance (p= 0.067). LDLR- patients presented higher WC, even after correction for sex, and much higher frequency of diabetes. In the whole group 26 patients had diabetes and interestingly all of them in the LDLR- group. Conclusions: The exclusion of LDLR and APOB genes mutations is recommended in subjects with the clinical diagnosis of FCH and high LDL cholesterol or Non HDL cholesterol. FH diagnostic criteria should not excluded subjects with triglycerides over 200 mg/dL.
PO40-630
EFFECT OF GARLIC ON HEMOSTASIS IN HYPERLIPIDEMIA
Mohammad 1 ,
Hadi 2 . 1 Clinical
B.I. N.R. Pharmacology,Diwaniyah College of Medicine,Diwaniyah, Iraq; 2 Clinical Pharmacology,Kufa College of Medicine,Kufa, Iraq Objectives: The effects of garlic on selected haemostatic parameters in hyperlipidemic rats. Method: Thirty rats were allocated randomly into 3 groups, each of 10 rats. One group was put on normal chow diet and acted as normal control group. The other 2 groups were given high cholesterol diet for 8 weeks. Both groups become hyperlipidemic after 8 weeks. One group received distilled water and acted as hyperlipidemic control while the other group received garlic (200mg/kg) for 4 weeks. Coagulation parameters and lipid profile were measured after 4 weeks of treatment with garlic. Blood samples were collected from all rats before and after commencement of the treatment to measure coagulation parameters, namely fibrinogen, activated partial thromboplastin time (APTT), prothrombin time (PT) and platelet count as well as serum lipid profile, namely total serum cholesterol (S.TC), serum triglyceride (S.TG), high density lipoprotein (HDL), and low density lipoprotein (LDL). These parameters were also measured after high cholesterol diet feeding. Results: The results indicated that a significant reduction in fibrinogen, S.TC, S.TG, LDL (p<0.01); an increase in HDL (p<0.01); a prolongation in PT and APTT (p<0.01) and a significant decrease in platelet count (p<0.01) in hyperlipidemic rats after 4 weeks of treatment with garlic. Conclusion: Garlic seems to be a favourable antiatherogenic and antithrombotic agent and is suggested to be used with food in patients with hyperlipidemia.
PO41 SCREENING FOR CV RISK – CIRCULATING MARKERS PO41-632
IN PATIENTS WITH TYPE 2 DIABETES LOWER PLASMA LEVEL OF MATRIX GLA PROTEIN IS ASSOCIATED WITH FASTER AORTIC STENOSIS PROGRESSION
D. Mohty, N. Cote, P. Pibarot, P. Moreau, P. Voisine, P. Poirier, P. Mathieu. Research Group in Vlavular Heart Diseases, Hopital Laval Research Center, Quebec, Quebec, Canada Background: Aging and diabetes are major risk factors for calcification of cardiovascular tissues. Aortic valve calcification is an independent predictor of faster hemodynamic progression in patient with AS. Matrix Gla protein (MGP) is an important inhibitor of calcification. The aim of the study was to determine whether plasma MGP level may interfere with valvular calcification and thereby with the hemodynamic progression of AS in diabetic patients. Methods: Plasma MGP level was determined by ELISA in 39 diabetics patients with severe AS (mean age: 72±9 y-o; 28 males; mean aortic valve area [AVA]: 0.75±0.14 cm2 ). The annualized progression rate of peak gradient and aortic valve area (AVA) was measured by Doppler-echocardiography. Results: 39 patients were divided into 2 groups according to the median value of MGP. The preoperative progression rates of the peak transvalvular gradient and AVA were significantly faster in patients with plasma MGP level <5.7 nmol/L when compared to those with higher level of MGP (10±5 vs.4±4 mmHg/year, p=0.01 and -0.2±0.1 vs.-0.06±0.05 cm2 /year, p=0.0007). After adjustment for age, gender and bicuspid valves reduced level of MGP remained significantly associated with the hemodynamic progression of the peak transvalvular gradients and the AVA (r2=0.31, β=2.71, p=0.04 and r2=0.49, β=-0.08, p=0.003) respectively. Conclusion: In patients with diabetes and severe AS, reduced plasma level of MGP may contribute to accelerated stenosis progression. This effect may, at least in part, be due to the loss of the inhibitory effect of circulating MGP on tissue calcification.
77th Congress of the European Atherosclerosis Society, April 26–29, 2008, Istanbul, Turkey
172 PO40-629
HYPOLIPIDEMIC EFFECT OF ARONIA MELANOCARPA FRUIT JUICE IN STREPTOZOTOCIN-INDUCED DIABETIC RATS
PO40-631
MUTATIONS IN THE LDLR AND APOB IN SUBJECTS WITH THE CLINICAL DIAGNOSIS OF FAMILIAL COMBINED HYPERLIPERLIPIDEMIA
S.V. Valcheva-Kuzmanova 1 , K.A. Kuzmanov 2 , S. Tancheva 3 , A. Belcheva 1 . 1 Department of Preclinical and Clinical Pharmacology and Biochemistry, Medical University, Varna, Bulgaria; 2 Vivarium, Medical University, Varna, Bulgaria; 3 Department of Clinical Laboratory, Medical University, Varna, Bulgaria
F. Civeira 1 , E. Jarauta 1 , A. Cenarro 1 , A.L. Garcia-Otin 1 , E. Ros 2 , D. Tejedor 3 , M. Pocovi 4 . 1 Hospital Universitario Miguel Servet, Zaragoza, Spain; 2 Hospital Clinic, Barcelona, Spain; 3 Progenika Biopharma S.A. Derio, Spain; 4 Departamento de Bioquimica, Biologia Molecular y Celular, Universidad de Zaragoza, Zaragoza, Spain
The increased risk for developing premature atherosclerosis in diabetes is due to increase in plasma triglycerides (TG) and low-density lipoproteins (LDL) and decrease in high-density lipoproteins (HDL). Aronia melanocarpa fruit juice (AMFJ) is rich in phenolic antioxidants, especially flavonoids from the anthocyanin subclass. The aim of the present study was to investigate the influence of AMFJ on plasma lipids in diabetic rats. Diabetes was induced by an intraperitoneal injection of streptozotocin (50 mg/kg). AMFJ was applied by gavage at doses of 10 and 20 ml/kg for six weeks to normal and diabetic rats. Streptozotocin application resulted in a significant elevation of plasma glucose by 141% in comparison with normal rats. This effect was accompanied by a significant elevation of plasma TG by 64%, statistically insignificant elevations of total cholesterol and LDL-cholesterol, and a reduction of HDL-cholesterol. Applied to normal rats, AMFJ did not influence plasma glucose and lipid levels. Applied to diabetic rats, the two AMFJ doses reduced significantly plasma TG by 35% and 39%, respectively, to levels that did not significantly differ from those of normal rats and counteracted the influence of streptozotocin on total cholesterol, LDL-cholesterol and HDL-cholesterol. This effect of AMFJ (10 and 20 ml/kg) on plasma lipids was probably due to improvement of the diabetic condition estimated by the significant reduction of plasma glucose by 44% and 42%, respectively. In conclusion, AMFJ reduced the abnormalities in blood lipids in diabetic rats and might be useful in prevention of diabetes-associated atherosclerosis.
Background and aims: To describe the frequency of LDLR and APOB mutations responsible of familial hyperholesteromia (FH) in a group of unrelated subjects with the diagnosis of familial combined hyperlipidemia (FCH) due to presence of mixed hyperlipidemia, familial presentation and absence of tendon xanthomas. Methods: 142 unrelated patients, aged >18 years, with the diagnosis of FCH were selected. The screening of mutations in the LDLR and APOB genes was performed with the Lipochip® , a microarray that includes the diagnosis of 203causative mutations in the LDLR gene and 4 mutations in APOB. Results: 92 men and 50 women were included. In 28 of them (19.7%) a functional mutation in the LDLR was found. Patients with LDLR mutation (LDLR+) had higher total cholesterol, Non HDL cholesterol and apo B concentrations than patients without mutation (LDLR-). However, triglycerides concentration was almost significantly higher in LDLR- than in LDL+ subjects (p=0.063). The frequency of mutation in women was 28% (14 out of 50), higher than in men (15.6%), although this difference did not reach statistical significance (p= 0.067). LDLR- patients presented higher WC, even after correction for sex, and much higher frequency of diabetes. In the whole group 26 patients had diabetes and interestingly all of them in the LDLR- group. Conclusions: The exclusion of LDLR and APOB genes mutations is recommended in subjects with the clinical diagnosis of FCH and high LDL cholesterol or Non HDL cholesterol. FH diagnostic criteria should not excluded subjects with triglycerides over 200 mg/dL.
PO40-630
EFFECT OF GARLIC ON HEMOSTASIS IN HYPERLIPIDEMIA
Mohammad 1 ,
Hadi 2 . 1 Clinical
B.I. N.R. Pharmacology,Diwaniyah College of Medicine,Diwaniyah, Iraq; 2 Clinical Pharmacology,Kufa College of Medicine,Kufa, Iraq Objectives: The effects of garlic on selected haemostatic parameters in hyperlipidemic rats. Method: Thirty rats were allocated randomly into 3 groups, each of 10 rats. One group was put on normal chow diet and acted as normal control group. The other 2 groups were given high cholesterol diet for 8 weeks. Both groups become hyperlipidemic after 8 weeks. One group received distilled water and acted as hyperlipidemic control while the other group received garlic (200mg/kg) for 4 weeks. Coagulation parameters and lipid profile were measured after 4 weeks of treatment with garlic. Blood samples were collected from all rats before and after commencement of the treatment to measure coagulation parameters, namely fibrinogen, activated partial thromboplastin time (APTT), prothrombin time (PT) and platelet count as well as serum lipid profile, namely total serum cholesterol (S.TC), serum triglyceride (S.TG), high density lipoprotein (HDL), and low density lipoprotein (LDL). These parameters were also measured after high cholesterol diet feeding. Results: The results indicated that a significant reduction in fibrinogen, S.TC, S.TG, LDL (p<0.01); an increase in HDL (p<0.01); a prolongation in PT and APTT (p<0.01) and a significant decrease in platelet count (p<0.01) in hyperlipidemic rats after 4 weeks of treatment with garlic. Conclusion: Garlic seems to be a favourable antiatherogenic and antithrombotic agent and is suggested to be used with food in patients with hyperlipidemia.
PO41 SCREENING FOR CV RISK – CIRCULATING MARKERS PO41-632
IN PATIENTS WITH TYPE 2 DIABETES LOWER PLASMA LEVEL OF MATRIX GLA PROTEIN IS ASSOCIATED WITH FASTER AORTIC STENOSIS PROGRESSION
D. Mohty, N. Cote, P. Pibarot, P. Moreau, P. Voisine, P. Poirier, P. Mathieu. Research Group in Vlavular Heart Diseases, Hopital Laval Research Center, Quebec, Quebec, Canada Background: Aging and diabetes are major risk factors for calcification of cardiovascular tissues. Aortic valve calcification is an independent predictor of faster hemodynamic progression in patient with AS. Matrix Gla protein (MGP) is an important inhibitor of calcification. The aim of the study was to determine whether plasma MGP level may interfere with valvular calcification and thereby with the hemodynamic progression of AS in diabetic patients. Methods: Plasma MGP level was determined by ELISA in 39 diabetics patients with severe AS (mean age: 72±9 y-o; 28 males; mean aortic valve area [AVA]: 0.75±0.14 cm2 ). The annualized progression rate of peak gradient and aortic valve area (AVA) was measured by Doppler-echocardiography. Results: 39 patients were divided into 2 groups according to the median value of MGP. The preoperative progression rates of the peak transvalvular gradient and AVA were significantly faster in patients with plasma MGP level <5.7 nmol/L when compared to those with higher level of MGP (10±5 vs.4±4 mmHg/year, p=0.01 and -0.2±0.1 vs.-0.06±0.05 cm2 /year, p=0.0007). After adjustment for age, gender and bicuspid valves reduced level of MGP remained significantly associated with the hemodynamic progression of the peak transvalvular gradients and the AVA (r2=0.31, β=2.71, p=0.04 and r2=0.49, β=-0.08, p=0.003) respectively. Conclusion: In patients with diabetes and severe AS, reduced plasma level of MGP may contribute to accelerated stenosis progression. This effect may, at least in part, be due to the loss of the inhibitory effect of circulating MGP on tissue calcification.
77th Congress of the European Atherosclerosis Society, April 26–29, 2008, Istanbul, Turkey