- Email: [email protected]
Immunohistochemical Expression of TGF-β1 in Feline and Canine Mammary Lesions
122
ESVP and ECVP Proceedings 2016
CANINE MAMMARY TUMOUR WITH SMOOTH AND STRIATED MUSCLE, OSSEOUS AND CHONDROID DIFFERENTIATION: UNUSUAL MIXED TYPE OR MESENCHYMOMA? A. Brunetti *, G. Beha *, L.V. Muscatello *, L. De Tolla y and G. Avallone* *Department of Veterinary Medical Sciences, University of Bologna, Italy and y University of Maryland, School of Medicine, Baltimore, MA, USA Introduction: Multiple mesenchymal components, usually represented by bone, cartilage, and adipose tissue, are often part of canine mixed mammary tumours. On the other hand, mesenchymomas are rare mesenchymal neoplasms demonstrating more than two mesenchymal components. The purpose of this investigation is to describe the histopathological features of a canine mammary tumour with multiple mesenchymal components. Materials and Methods: Two nodules, respectively in the right cranial thoracic gland (M1) and in the left inguinal gland (M5), were removed from a 13-year-old crossbreed female dog. PTAH stain and immunohistochemistry using a panel of antibodies (anti-smooth muscle actin [SMA], -desmin, -calponin, -P63, -CK14, -CK19 and -vimentin) was applied to M5. Results: M5 was a well-demarcated, non-encapsulated, expansive, moderately cellular neoplasm composed of well-differentiated mesenchymal components (i.e. cartilage, bone, smooth and striated muscle) and !5% of mammary epithelium at the periphery of the lesion. All mesenchymal areas of the tumour expressed vimentin. Striated muscle expressed desmin and calponin and smooth muscle expressed desmin, SMA and calponin. PTAH demonstrated cross striation of striated muscle. The epithelial component was CK19 positive and was associated with a layer of myoepithelium expressing calponin, CK14 and P63. M1 was diagnosed histologically as carcinoma-mixed type. Conclusions: Small areas of epithelial cells confirmed the mammary origin of M5. However, the nature of the tumour remains uncertain; either it was an unusual benign mixed tumour with muscle differentiation, presumably arising from the myoepithelium, or a rare benign mesenchymoma infiltrating the mammary gland.
J. Comp. Path. 2017, Vol. 156, 54e141
IMMUNOHISTOCHEMICAL EXPRESSION OF TGF-b1 IN FELINE AND CANINE MAMMARY LESIONS J. Pina, A. Gama, M.A. Pires and F. Seixas CECAV, Animal and Veterinary Research Centre, Laboratory of Histology and Surgical Pathology, Department of Veterinary Sciences, University of Tras-osMontes e Alto Douro, Vila Real, Portugal Introduction: Spontaneously arising mammary tumours are common in female cats and dogs. The transforming growth factor (TGF)-b superfamily is expressed ubiquitously in diverse tissues. The TGF-b1 isoform regulates cell proliferation, differentiation, migration, adhesion and death; some studies suggest that it functions both as a tumour suppressor and promoter. The aim of this work was to perform a comparative study of TGF-b1 expression in feline and canine non-neoplastic and neoplastic tissues, contributing to their biopathological characterization. Materials and Methods: We analyzed 99 samples of feline and 116 samples of canine mammary tissues obtained from UTAD’s Pathology Laboratory. Normal mammary tissue from animals devoid of mammary lesions was used as control. TGF-b1 immunohistochemistry was performed by the modified avidinebiotineperoxidase complex method. Results: In the queen, most of the histological groups showed high expression of TGF-b1 by epithelium, but low expression in the stroma. In canine tissues, the epithelial expression of TGF-b1 was low in most samples, regardless of the histological group. In the stroma, significant differences were achieved, with benign neoplasia showing low expression levels, in contrast with carcinomas (P !0.0001). Significant differences were observed between feline and canine malignant tissues, with most feline carcinomas exhibiting high epithelial and low stromal TGF-b1 expression and canine tissues with opposite findings. Conclusions: This study suggests that it is not the amount of TGFb1 available in the tumour environment that promotes tumour progression, and that crosstalk with other pathways may play an important role in TGF-b1 signalling.
ESVP and ECVP Proceedings 2016
CANINE MAMMARY TUMOUR WITH SMOOTH AND STRIATED MUSCLE, OSSEOUS AND CHONDROID DIFFERENTIATION: UNUSUAL MIXED TYPE OR MESENCHYMOMA? A. Brunetti *, G. Beha *, L.V. Muscatello *, L. De Tolla y and G. Avallone* *Department of Veterinary Medical Sciences, University of Bologna, Italy and y University of Maryland, School of Medicine, Baltimore, MA, USA Introduction: Multiple mesenchymal components, usually represented by bone, cartilage, and adipose tissue, are often part of canine mixed mammary tumours. On the other hand, mesenchymomas are rare mesenchymal neoplasms demonstrating more than two mesenchymal components. The purpose of this investigation is to describe the histopathological features of a canine mammary tumour with multiple mesenchymal components. Materials and Methods: Two nodules, respectively in the right cranial thoracic gland (M1) and in the left inguinal gland (M5), were removed from a 13-year-old crossbreed female dog. PTAH stain and immunohistochemistry using a panel of antibodies (anti-smooth muscle actin [SMA], -desmin, -calponin, -P63, -CK14, -CK19 and -vimentin) was applied to M5. Results: M5 was a well-demarcated, non-encapsulated, expansive, moderately cellular neoplasm composed of well-differentiated mesenchymal components (i.e. cartilage, bone, smooth and striated muscle) and !5% of mammary epithelium at the periphery of the lesion. All mesenchymal areas of the tumour expressed vimentin. Striated muscle expressed desmin and calponin and smooth muscle expressed desmin, SMA and calponin. PTAH demonstrated cross striation of striated muscle. The epithelial component was CK19 positive and was associated with a layer of myoepithelium expressing calponin, CK14 and P63. M1 was diagnosed histologically as carcinoma-mixed type. Conclusions: Small areas of epithelial cells confirmed the mammary origin of M5. However, the nature of the tumour remains uncertain; either it was an unusual benign mixed tumour with muscle differentiation, presumably arising from the myoepithelium, or a rare benign mesenchymoma infiltrating the mammary gland.
J. Comp. Path. 2017, Vol. 156, 54e141
IMMUNOHISTOCHEMICAL EXPRESSION OF TGF-b1 IN FELINE AND CANINE MAMMARY LESIONS J. Pina, A. Gama, M.A. Pires and F. Seixas CECAV, Animal and Veterinary Research Centre, Laboratory of Histology and Surgical Pathology, Department of Veterinary Sciences, University of Tras-osMontes e Alto Douro, Vila Real, Portugal Introduction: Spontaneously arising mammary tumours are common in female cats and dogs. The transforming growth factor (TGF)-b superfamily is expressed ubiquitously in diverse tissues. The TGF-b1 isoform regulates cell proliferation, differentiation, migration, adhesion and death; some studies suggest that it functions both as a tumour suppressor and promoter. The aim of this work was to perform a comparative study of TGF-b1 expression in feline and canine non-neoplastic and neoplastic tissues, contributing to their biopathological characterization. Materials and Methods: We analyzed 99 samples of feline and 116 samples of canine mammary tissues obtained from UTAD’s Pathology Laboratory. Normal mammary tissue from animals devoid of mammary lesions was used as control. TGF-b1 immunohistochemistry was performed by the modified avidinebiotineperoxidase complex method. Results: In the queen, most of the histological groups showed high expression of TGF-b1 by epithelium, but low expression in the stroma. In canine tissues, the epithelial expression of TGF-b1 was low in most samples, regardless of the histological group. In the stroma, significant differences were achieved, with benign neoplasia showing low expression levels, in contrast with carcinomas (P !0.0001). Significant differences were observed between feline and canine malignant tissues, with most feline carcinomas exhibiting high epithelial and low stromal TGF-b1 expression and canine tissues with opposite findings. Conclusions: This study suggests that it is not the amount of TGFb1 available in the tumour environment that promotes tumour progression, and that crosstalk with other pathways may play an important role in TGF-b1 signalling.