- Email: [email protected]
or osteoporosis
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Abstracts / Bone 48 (2011) S187–S203
dwelling Japanese elderly men. However, this unexpected finding turned out to be insignificant when assessing renal function with Cys-c. eGFR may be misleading in evaluating the association between subclinical renal dysfunction and BMD. Acknowledgements: The FORMEN study was performed as ancillary study of The Fujiwara-kyo Study, and supported by the Japanese Society for Promotion of Science (#20659103, #21390210) and the Japan Dairy Association (2008).
This article is part of a Special Issue entitled ECTS 2011. Disclosure of interest: None declared. doi:10.1016/j.bone.2011.03.692
PP306-T The relationship between timed up and go test, calcidiol levels, and history of falls and fractures in osteoporotic postmenopausal women M. Bernad Pineda a, ⁎, C. Gonzalez Fernandez b, M. Fernandez Prada c, J. Fernandez Campillo d, R. Maeso Martin e, M.V. Garces Puentes f a Rheumatology, La Paz University Hospital, Madrid, Spain b Rheumatology, University Hospital Gregorio Marañon, Madrid, Spain c Rheumatology, Guadalajara Hospital, Guadalajara, Spain d Rheumatology, Torrevieja Hospital, Alicante, Spain e Medical, Rovi Pharmaceutical Laboratory, Madrid, Spain f Integral Services Medical Management, Madrid, Spain Abstract: Objective: To evaluate the relationship between Timed up and Go test (TUG), calcidiol levels and history of falls and fractures in a cohort of osteoporotic postmenopausal women (OPW) from RETOSS study in Spain. Methods: An observational, transverse and multicentric study was conducted on 629 OPW previously diagnosed in 63rheumatology divisions. Every physician had to choose only one patient per day who was the first woman to come and to fulfill with the inclusion and exclusion criteria. Women calcidiol levels, falls at last year and history of fractures were recorded. TUG test1 was performed. Results: Women mean age and TUG test was 66.6 ± 9.2 years and 16.3 ± 11.3 s (95% CI, 15.3–17.3), respectively. Mean TUG test time showed significant differences between women with history of falls and fractures (18.1 s and 19.0 s) and others non fallers and without fractures (15.6 s and 14.4 s) (p = 0.024 and p < 0.001), respectively. TUG test showed a linear trend to an increased probability > 20 s in relationship with the age (p < 0.001) and with an increased estimated risk of falls (OR:1.72; 95% CI, 1.13–2.63) and fractures, too (OR:2.54; 95% CI, 1.69–3.80). 31.2% of patients fell during the last year and 51.5% had a consequence fracture. It was found a significant association between falls and TUG test > 20 s in patients > 70 years (p = 0.025; OR: 2.90; 95% CI, 1.13–7.45). There was a significant inverse association between history of fractures and calcidiol level age independent. Patients who had calcidiol < 20 ng/mL sustained more falls (p = 0.033) and fractures (p = 0.006) than women with calcidiol > 20 ng/mL. Conclusion: TUG test is statistically associated with a history of falls and fractures in OPW. TUG is quick, requires no special equipment or training, and may also be useful to include as part of the routine medical examination in old patients. This article is part of a Special Issue entitled ECTS 2011. Disclosure of interest: None Declared. Reference [1] Podsiadlo D, Richardson S. J Am Geriatr Soc 1991;39:142–8. doi:10.1016/j.bone.2011.03.693
PP307-S Increased oxidative stress is associated with altered bone health among postmenopausal women with hypercholesterolaemia M.-S.M. Ardawi a, ⁎, M.H. Qari b, D.H. Akbar c, A.A. AlShaikh c, S.A. Al-Sibiani d, W.M. Faqeeh d, A.A. Rouzi d a Department of Clinical Biochemistry, Center of Excellence for Osteoporosis Research, Faculty of Medicine and KAU Hospital, King Abdulaziz University, Jeddah, Saudi Arabia b Department of Hematology, Center of Excellence for Osteoporosis Research, Faculty of Medicine and KAU Hospital, King Abdulaziz University, Jeddah, Saudi Arabia c Department of Internal Medicine, Center of Excellence for Osteoporosis Research, Faculty of Medicine and KAU Hospital, King Abdulaziz University, Jeddah, Saudi Arabia d Department of Obstetrics & Gynecology, Center of Excellence for Osteoporosis Research, Faculty of Medicine and KAU Hospital, King Abdulaziz University, Jeddah, Saudi Arabia Abstract: Introduction: Although the mechanisms involved in the pathogenesis of osteoporosis are not fully understood, there is evidence to suggest that oxidative stress due to reactive oxygen species (ROS) is important. The relationship between bone mass and lipid has been shown to be negative for low-density lipoprotein cholesterol (LDL-c) and positive for high density lipoprotein cholesterol (HDL-c); however, others observed an opposite relationship between lipid profile and bone mineral density (BMD). Recent studies suggest a possible link between hypercholesterolaemia and osteoporosis. Objectives: To examine the relationship between oxidative stress parameters, BMD, bone turnover markers (BTMs), osteoprotegerin (OPG) and receptor activator of nuclear factor-kappa-B ligand (RANKL) in postmenopausal women with hypercholesterolaemia. Subjects and Methods: A total of 305 postmenopausal women with hypercholesterolaemia (age ≥ 50–75 years) provided blood and urine samples. Serum protein thiols, lipid peroxidation, total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), BTMs (bone formation markers: s-OC and s-PINP; bone resorption markers: s-CTx and u-NTx) together with BMD [at five bone sites measured by dualenergy X-ray absorptiometry (DXA)] were measured in relation to lipid parameters including: total cholesterol, LDL-c, HDL-c and triglycerides. A total of 305 age- and bodymass-index-matched normocholesterolemic postmenopausal women were used as controls. Results: Women with hypercholesterolaemia exhibited higher (P < 0.001) protein oxidation, lipid peroxidation and OSI levels than controls. They also showed decreased (P < 0.000) femoral neck, Wards', trochanter, total hip and lumbar spine (L1– L4) BMD values and lower bone formation (s-OC and s-PINP) (P < 0.001) and higher bone resorption (s-CTx and u-NTx) (P < 0.05) markers values. No differences were observed for serum OPG and RANKL levels. In multivariate linear regression analysis, protein oxidation, lipid peroxidation and OSI were the only negative predictors of BMD and BTMs values in women with hypercholesterolaemia. Conclusions: An association between increased protein oxidation, lipid peroxidation and OSI and lower BMD and BTMs values were evident in postmenopausal women with hypercholesterolaemia. These results suggest a possible role for oxidative stress in the development of reduced bone mass among postmenopausal women with hypercholesterolaemia. This article is part of a Special Issue entitled ECTS 2011. Disclosure of interest: None declared.
doi:10.1016/j.bone.2011.03.694
PP308-M Increased serum osteopontin is a risk factor for low bone mineral density and/or osteoporosis M.-S.M. Ardawi a, ⁎, M.H. Qari b, A.A. Rouzi c, W.M. Faqeeh c, S.A. Al-Sibiani c, D.H. Akbar d, A.A. AlShaikh d a Department of Clinical Biochemistry, Center of Excellence for Osteoporosis Research, Center of Excellence for Osteoporosis Research, Faculty of Medicine and KAU Hospital, King Abdulaziz University, Jeddah, Saudi Arabia b Department of Hematology, Center of Excellence for Osteoporosis Research, Center of Excellence for Osteoporosis Research, Faculty of Medicine and KAU Hospital, King Abdulaziz University, Jeddah, Saudi Arabia c Department of Obstetrics & Gynecology, Center of Excellence for Osteoporosis Research, Center of Excellence for Osteoporosis Research, Faculty of Medicine and KAU Hospital, King Abdulaziz University, Jeddah, Saudi Arabia
Abstracts / Bone 48 (2011) S187–S203 d
Department of Internal Medicine, Center of Excellence for Osteoporosis Research, Faculty of Medicine and KAU Hospital, King Abdulaziz University, Jeddah, Saudi Arabia
Abstract: Introduction: Osteopontin (OPN) is a phosphoprotein and considered to be a non-collagenous bone matrix expressed in both osteoblasts and osteoclasts and was found to be associated with bone strength and remodeling. Several experimental animal studies showed that OPN-deficient mice are resistant to ovariectomy-induced osteoporosis. Objective: To examine the relationship between serum OPN levels and bone mineral density (BMD), bone turnover markers (BTMs), serum 25-hdyroxyvitamin D [25(OH)D], parathyroid hormone (intact-PTH) and other variables among healthy pre- and postmenopausal women. Subjects and Methods: A total of 1012 healthy Saudi women (age 25– 75 years) living in the Jeddah area were randomly selected, completed a questionnaire and provided blood and urine samples. Anthropometric parameters, socioeconomic status, together with serum levels of OPN, 25(OH)D, intact-PTH, estradiol (E2), calcium (Ca), phosphate (PO4), magnesium (Mg), and BTMs were measured. BMD was measured by a dual energy X-ray absorptiometry (DXA). Results: In postmenopausal women, serum OPN levels exhibited significant positive correlations with age (P < 0.000) and BTMs (P < 0.001) and negative correlations with body weight (P < 0.01), height (P < 0.001), serum 25(OH)D (P < 0.05) and BMD [lumbar spine (L1–L4); neck femur and total hip] (P < 0.001). Conversely, in premenopausal women, serum E2 and body height showed significant positive correlations with OPN with no correlations with other variables examined. After adjustment for confounders, the odds ratio (OR) for osteopenia (OR = 1.95; CI: 0.90–5.32) and osteoporosis (OR = 3.11; CI: 1.13–9.26) were observed in postmenopausal women with the highest quintile of serum OPN, respectively. Conclusions: Osteopontin plays a role in human bone health and serum OPN levels could be used as a biomarker for the early diagnosis of low bone mass and/or osteoporosis in postmenopausal women. This article is part of a Special Issue entitled ECTS 2011. Disclosure of interest: None declared.
doi:10.1016/j.bone.2011.03.695
PP309-T Lycopene intake in relation to bone mineral density, bone turnover markers and oxidative stress status in healthy postmenopausal women: A cross-sectional study M.-S.M. Ardawi a, ⁎, M.H. Qari b, A.A. Rouzi c, W.M. Faqeeh c, S.A. Al-Sibiani c a Department of Clinical Biochemistry, Center of Excellence for Osteoporosis Research and Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia b Department of Hematology, Center of Excellence for Osteoporosis Research and Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia c Department of Obstetrics and Gynecology, Center of Excellence for Osteoporosis Research and Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia Abstract: Introduction: Lycopene is an antioxidant known to decrease the risk of agerelated chronic diseases and recent evidence suggests that it has a protective effect on bone. Oxidative stress induced by reactive oxygen species (ROS) is associated with the risk of bone loss and osteoporosis, and can be reduced by certain antioxidants such as lycopene. Objectives: To assess the effect of lycopene intake on bone mineral density (BMD) and bone turnover markers (BTMs) in relation to oxidative stress parameters among healthy Saudi postmenopausal women. Subjects and Methods: A total of 440 healthy women (aged ≥ 50–65) years provided 4-day dietary records, blood and urine samples. Serum samples were used to measure serum lycopene, protein thiols, lipid peroxidation, total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), BMD [at five bone sites measured by dual-energy X-ray absorptiometry (DXA)] and BTMs (bone formation markers: s-OC and s-PINP; bone resorption markers: s-CTx and u-NTx). Women were stratified according to serum lycopene (nmol/kg body wt) quintiles (Q1–Q5) and examined in relation to the above variables. Results: Higher dietary lycopene intake exhibited higher serum lycopene (P < 0.000) and positive effects were demonstrated on bone health: significant increases in BMD values (by 4.8–9.1%), increases in bone formation (by 13.0–32.1%); and decreases in bone resorption (by 22.8–27.5%) markers in the highest quintile (Q5) of serum lycopene as compared with the lowest quintile (Q1) group. Such changes were accompanied by improved oxidative stress parameters (OSI decreased by 34.0%, P < 0.000) including increases in protein thiols (18.7%, P < 0.000) and decreases in lipid peroxidation (41.9%, P < 0.000) at higher serum lycopene levels. After adjustment for confounders, the odds ratio (OR) for the lowest quintile of BMD in the high groups (Q2–Q5) of serum lycopene versus the lowest quintile (Q1) was 0.44 (95% CI: 0.6–0.90) [for lumbar spine (L1–L4)] and 0.62 (95% CI: 0.17–0.95) (for neck femur), respectively. Conclusions: These results suggest that dietary antioxidant lycopene decreases oxidative stress and the levels of bone resorption markers and increases antioxidative status and bone formation
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markers in postmenopausal women, and may be beneficial in decreasing the risk of bone loss and/or osteoporosis. This article is part of a Special Issue entitled ECTS 2011. Disclosure of interest: None declared.
doi:10.1016/j.bone.2011.03.696
PP310-S Use of oral platelet inhibitors increases risk of fractures N.R. Jorgensen a, ⁎, T.H. Steinberg b, P. Schwarz c, P. Vestergaard d a Department of Clinical Biochemistry, Copenhagen University Hospital Glostrup, Glostrup, Denmark b Dept. of Internal Medicine, Washington University, St. Louis, USA c Research Center for Ageing and Osteoporosis, Copenhagen University Hospital Glostrup, Glostrup, Denmark d Osteoporosis Clinic, Dept. of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark Abstract: Background: Platelet inhibitors are widely used in the treatment and prevention of coronary artery disease. In addition to aspirin, two major groups of platelet inhibitors are used; Phosphodiesterase inhibitors including dipyridamole, and thienopyridines, to which ticlopidine and clopidogrel belong. Clopidogrel is the most widely used, and in combination with aspirin it is the standard of care for acute coronary syndromes and percutaneous coronary interventions. However, the modes of actions of these compounds involve pathways that are also involved in the metabolic activity in bone cells and pharmacologic modulation of these pathways may therefore have effects on bone turnover. Methods: In the current study, we assessed the effects of platelet inhibitors (dipyridamole and low-dose acetylsalicylic acid) on fracture incidence in a population-based epidemiological study performed within the Danish population consisting of approximately 5.3 million individuals, where all patients sustaining a fracture during the year of 2000 were included (124,655 cases). The hypotheses were to investigate if use of phosphodiesterase inhibitors and acetylsalicylic acid were associated with an increased risk of fractures after adjustment for potential confounders. Results: We found that treatment with dipyridamole is associated with increased overall fracture risk, but not to the risk of osteoporotic fractures. In contrast, low-dose ASA is associated to increased risk of both overall fractures and fractures of the hip. Conclusions: Use of oral platelet inhibitors increases the risk of fractures. When initiating treatment with oral platelet inhibitors, bone status should ideally also be assessed. This article is part of a Special Issue entitled ECTS 2011. Disclosure of interest: None declared.
doi:10.1016/j.bone.2011.03.697
PP311-M Evidence of a link between poor bone health, low vitamin D status and CVD risk in caucasian and asian women O. Hakim a, ⁎, F. Shojaee-Moradie b, K. Hart a, J. Berry c, R. Eastell d, F. Gossiel d, R. Hannon d, M. Umpleby b, B. Griffin a, S. Lanham-New a a Nutritional Sciences Division, University of Surrey, Guildford, UK b Postgraduate Medical School, University of Surrey, Guildford, UK c Department of Medicine, University of Manchester, Manchester, UK d Department of Human Metabolism, University of Sheffield, Sheffield, UK Abstract: Osteoporosis has been linked to CVD risk, suggesting that there may be an association between a poor serum lipid profile and increased bone resorption/lower bone mass. There are also data linking vitamin D ‘insufficiency’ with increased CVD. The aim of this study was to examine the association between bone health, low vitamin D status and CVD lipid profiles. The D-FINES study (Vitamin D, Food Intake, Nutrition and Exposure to Sunlight in Southern England) investigated the interaction between diet and sunlight exposure on vitamin D status. Fasted blood sample were collected at three monthly intervals from Summer 2006 to Spring 2007 on a total of 223 Caucasian (C) and 70 Asian (A) women aged 19–70 years living in Southern England. Serum CTx was assessed and parathyroid hormone (PTH) was measured. Bone quality including broadband ultrasound attenuation (BUA), velocity of sound (VOS), lumbar spine (LS) BMD, and femoral neck (FN) BMD in Autumn 2006 and Spring of 2007 were assessed. The aim of the present subsidiary study was to examine the association between bone health (bone quality, bone resorption and PTH), vitamin D status, and CVD. Correlation analysis between serum lipid profiles (serum triglyceride (TAG), total cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL)) and bone health parameters and vitamin D status have been measured.
Abstracts / Bone 48 (2011) S187–S203
dwelling Japanese elderly men. However, this unexpected finding turned out to be insignificant when assessing renal function with Cys-c. eGFR may be misleading in evaluating the association between subclinical renal dysfunction and BMD. Acknowledgements: The FORMEN study was performed as ancillary study of The Fujiwara-kyo Study, and supported by the Japanese Society for Promotion of Science (#20659103, #21390210) and the Japan Dairy Association (2008).
This article is part of a Special Issue entitled ECTS 2011. Disclosure of interest: None declared. doi:10.1016/j.bone.2011.03.692
PP306-T The relationship between timed up and go test, calcidiol levels, and history of falls and fractures in osteoporotic postmenopausal women M. Bernad Pineda a, ⁎, C. Gonzalez Fernandez b, M. Fernandez Prada c, J. Fernandez Campillo d, R. Maeso Martin e, M.V. Garces Puentes f a Rheumatology, La Paz University Hospital, Madrid, Spain b Rheumatology, University Hospital Gregorio Marañon, Madrid, Spain c Rheumatology, Guadalajara Hospital, Guadalajara, Spain d Rheumatology, Torrevieja Hospital, Alicante, Spain e Medical, Rovi Pharmaceutical Laboratory, Madrid, Spain f Integral Services Medical Management, Madrid, Spain Abstract: Objective: To evaluate the relationship between Timed up and Go test (TUG), calcidiol levels and history of falls and fractures in a cohort of osteoporotic postmenopausal women (OPW) from RETOSS study in Spain. Methods: An observational, transverse and multicentric study was conducted on 629 OPW previously diagnosed in 63rheumatology divisions. Every physician had to choose only one patient per day who was the first woman to come and to fulfill with the inclusion and exclusion criteria. Women calcidiol levels, falls at last year and history of fractures were recorded. TUG test1 was performed. Results: Women mean age and TUG test was 66.6 ± 9.2 years and 16.3 ± 11.3 s (95% CI, 15.3–17.3), respectively. Mean TUG test time showed significant differences between women with history of falls and fractures (18.1 s and 19.0 s) and others non fallers and without fractures (15.6 s and 14.4 s) (p = 0.024 and p < 0.001), respectively. TUG test showed a linear trend to an increased probability > 20 s in relationship with the age (p < 0.001) and with an increased estimated risk of falls (OR:1.72; 95% CI, 1.13–2.63) and fractures, too (OR:2.54; 95% CI, 1.69–3.80). 31.2% of patients fell during the last year and 51.5% had a consequence fracture. It was found a significant association between falls and TUG test > 20 s in patients > 70 years (p = 0.025; OR: 2.90; 95% CI, 1.13–7.45). There was a significant inverse association between history of fractures and calcidiol level age independent. Patients who had calcidiol < 20 ng/mL sustained more falls (p = 0.033) and fractures (p = 0.006) than women with calcidiol > 20 ng/mL. Conclusion: TUG test is statistically associated with a history of falls and fractures in OPW. TUG is quick, requires no special equipment or training, and may also be useful to include as part of the routine medical examination in old patients. This article is part of a Special Issue entitled ECTS 2011. Disclosure of interest: None Declared. Reference [1] Podsiadlo D, Richardson S. J Am Geriatr Soc 1991;39:142–8. doi:10.1016/j.bone.2011.03.693
PP307-S Increased oxidative stress is associated with altered bone health among postmenopausal women with hypercholesterolaemia M.-S.M. Ardawi a, ⁎, M.H. Qari b, D.H. Akbar c, A.A. AlShaikh c, S.A. Al-Sibiani d, W.M. Faqeeh d, A.A. Rouzi d a Department of Clinical Biochemistry, Center of Excellence for Osteoporosis Research, Faculty of Medicine and KAU Hospital, King Abdulaziz University, Jeddah, Saudi Arabia b Department of Hematology, Center of Excellence for Osteoporosis Research, Faculty of Medicine and KAU Hospital, King Abdulaziz University, Jeddah, Saudi Arabia c Department of Internal Medicine, Center of Excellence for Osteoporosis Research, Faculty of Medicine and KAU Hospital, King Abdulaziz University, Jeddah, Saudi Arabia d Department of Obstetrics & Gynecology, Center of Excellence for Osteoporosis Research, Faculty of Medicine and KAU Hospital, King Abdulaziz University, Jeddah, Saudi Arabia Abstract: Introduction: Although the mechanisms involved in the pathogenesis of osteoporosis are not fully understood, there is evidence to suggest that oxidative stress due to reactive oxygen species (ROS) is important. The relationship between bone mass and lipid has been shown to be negative for low-density lipoprotein cholesterol (LDL-c) and positive for high density lipoprotein cholesterol (HDL-c); however, others observed an opposite relationship between lipid profile and bone mineral density (BMD). Recent studies suggest a possible link between hypercholesterolaemia and osteoporosis. Objectives: To examine the relationship between oxidative stress parameters, BMD, bone turnover markers (BTMs), osteoprotegerin (OPG) and receptor activator of nuclear factor-kappa-B ligand (RANKL) in postmenopausal women with hypercholesterolaemia. Subjects and Methods: A total of 305 postmenopausal women with hypercholesterolaemia (age ≥ 50–75 years) provided blood and urine samples. Serum protein thiols, lipid peroxidation, total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), BTMs (bone formation markers: s-OC and s-PINP; bone resorption markers: s-CTx and u-NTx) together with BMD [at five bone sites measured by dualenergy X-ray absorptiometry (DXA)] were measured in relation to lipid parameters including: total cholesterol, LDL-c, HDL-c and triglycerides. A total of 305 age- and bodymass-index-matched normocholesterolemic postmenopausal women were used as controls. Results: Women with hypercholesterolaemia exhibited higher (P < 0.001) protein oxidation, lipid peroxidation and OSI levels than controls. They also showed decreased (P < 0.000) femoral neck, Wards', trochanter, total hip and lumbar spine (L1– L4) BMD values and lower bone formation (s-OC and s-PINP) (P < 0.001) and higher bone resorption (s-CTx and u-NTx) (P < 0.05) markers values. No differences were observed for serum OPG and RANKL levels. In multivariate linear regression analysis, protein oxidation, lipid peroxidation and OSI were the only negative predictors of BMD and BTMs values in women with hypercholesterolaemia. Conclusions: An association between increased protein oxidation, lipid peroxidation and OSI and lower BMD and BTMs values were evident in postmenopausal women with hypercholesterolaemia. These results suggest a possible role for oxidative stress in the development of reduced bone mass among postmenopausal women with hypercholesterolaemia. This article is part of a Special Issue entitled ECTS 2011. Disclosure of interest: None declared.
doi:10.1016/j.bone.2011.03.694
PP308-M Increased serum osteopontin is a risk factor for low bone mineral density and/or osteoporosis M.-S.M. Ardawi a, ⁎, M.H. Qari b, A.A. Rouzi c, W.M. Faqeeh c, S.A. Al-Sibiani c, D.H. Akbar d, A.A. AlShaikh d a Department of Clinical Biochemistry, Center of Excellence for Osteoporosis Research, Center of Excellence for Osteoporosis Research, Faculty of Medicine and KAU Hospital, King Abdulaziz University, Jeddah, Saudi Arabia b Department of Hematology, Center of Excellence for Osteoporosis Research, Center of Excellence for Osteoporosis Research, Faculty of Medicine and KAU Hospital, King Abdulaziz University, Jeddah, Saudi Arabia c Department of Obstetrics & Gynecology, Center of Excellence for Osteoporosis Research, Center of Excellence for Osteoporosis Research, Faculty of Medicine and KAU Hospital, King Abdulaziz University, Jeddah, Saudi Arabia
Abstracts / Bone 48 (2011) S187–S203 d
Department of Internal Medicine, Center of Excellence for Osteoporosis Research, Faculty of Medicine and KAU Hospital, King Abdulaziz University, Jeddah, Saudi Arabia
Abstract: Introduction: Osteopontin (OPN) is a phosphoprotein and considered to be a non-collagenous bone matrix expressed in both osteoblasts and osteoclasts and was found to be associated with bone strength and remodeling. Several experimental animal studies showed that OPN-deficient mice are resistant to ovariectomy-induced osteoporosis. Objective: To examine the relationship between serum OPN levels and bone mineral density (BMD), bone turnover markers (BTMs), serum 25-hdyroxyvitamin D [25(OH)D], parathyroid hormone (intact-PTH) and other variables among healthy pre- and postmenopausal women. Subjects and Methods: A total of 1012 healthy Saudi women (age 25– 75 years) living in the Jeddah area were randomly selected, completed a questionnaire and provided blood and urine samples. Anthropometric parameters, socioeconomic status, together with serum levels of OPN, 25(OH)D, intact-PTH, estradiol (E2), calcium (Ca), phosphate (PO4), magnesium (Mg), and BTMs were measured. BMD was measured by a dual energy X-ray absorptiometry (DXA). Results: In postmenopausal women, serum OPN levels exhibited significant positive correlations with age (P < 0.000) and BTMs (P < 0.001) and negative correlations with body weight (P < 0.01), height (P < 0.001), serum 25(OH)D (P < 0.05) and BMD [lumbar spine (L1–L4); neck femur and total hip] (P < 0.001). Conversely, in premenopausal women, serum E2 and body height showed significant positive correlations with OPN with no correlations with other variables examined. After adjustment for confounders, the odds ratio (OR) for osteopenia (OR = 1.95; CI: 0.90–5.32) and osteoporosis (OR = 3.11; CI: 1.13–9.26) were observed in postmenopausal women with the highest quintile of serum OPN, respectively. Conclusions: Osteopontin plays a role in human bone health and serum OPN levels could be used as a biomarker for the early diagnosis of low bone mass and/or osteoporosis in postmenopausal women. This article is part of a Special Issue entitled ECTS 2011. Disclosure of interest: None declared.
doi:10.1016/j.bone.2011.03.695
PP309-T Lycopene intake in relation to bone mineral density, bone turnover markers and oxidative stress status in healthy postmenopausal women: A cross-sectional study M.-S.M. Ardawi a, ⁎, M.H. Qari b, A.A. Rouzi c, W.M. Faqeeh c, S.A. Al-Sibiani c a Department of Clinical Biochemistry, Center of Excellence for Osteoporosis Research and Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia b Department of Hematology, Center of Excellence for Osteoporosis Research and Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia c Department of Obstetrics and Gynecology, Center of Excellence for Osteoporosis Research and Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia Abstract: Introduction: Lycopene is an antioxidant known to decrease the risk of agerelated chronic diseases and recent evidence suggests that it has a protective effect on bone. Oxidative stress induced by reactive oxygen species (ROS) is associated with the risk of bone loss and osteoporosis, and can be reduced by certain antioxidants such as lycopene. Objectives: To assess the effect of lycopene intake on bone mineral density (BMD) and bone turnover markers (BTMs) in relation to oxidative stress parameters among healthy Saudi postmenopausal women. Subjects and Methods: A total of 440 healthy women (aged ≥ 50–65) years provided 4-day dietary records, blood and urine samples. Serum samples were used to measure serum lycopene, protein thiols, lipid peroxidation, total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), BMD [at five bone sites measured by dual-energy X-ray absorptiometry (DXA)] and BTMs (bone formation markers: s-OC and s-PINP; bone resorption markers: s-CTx and u-NTx). Women were stratified according to serum lycopene (nmol/kg body wt) quintiles (Q1–Q5) and examined in relation to the above variables. Results: Higher dietary lycopene intake exhibited higher serum lycopene (P < 0.000) and positive effects were demonstrated on bone health: significant increases in BMD values (by 4.8–9.1%), increases in bone formation (by 13.0–32.1%); and decreases in bone resorption (by 22.8–27.5%) markers in the highest quintile (Q5) of serum lycopene as compared with the lowest quintile (Q1) group. Such changes were accompanied by improved oxidative stress parameters (OSI decreased by 34.0%, P < 0.000) including increases in protein thiols (18.7%, P < 0.000) and decreases in lipid peroxidation (41.9%, P < 0.000) at higher serum lycopene levels. After adjustment for confounders, the odds ratio (OR) for the lowest quintile of BMD in the high groups (Q2–Q5) of serum lycopene versus the lowest quintile (Q1) was 0.44 (95% CI: 0.6–0.90) [for lumbar spine (L1–L4)] and 0.62 (95% CI: 0.17–0.95) (for neck femur), respectively. Conclusions: These results suggest that dietary antioxidant lycopene decreases oxidative stress and the levels of bone resorption markers and increases antioxidative status and bone formation
S197
markers in postmenopausal women, and may be beneficial in decreasing the risk of bone loss and/or osteoporosis. This article is part of a Special Issue entitled ECTS 2011. Disclosure of interest: None declared.
doi:10.1016/j.bone.2011.03.696
PP310-S Use of oral platelet inhibitors increases risk of fractures N.R. Jorgensen a, ⁎, T.H. Steinberg b, P. Schwarz c, P. Vestergaard d a Department of Clinical Biochemistry, Copenhagen University Hospital Glostrup, Glostrup, Denmark b Dept. of Internal Medicine, Washington University, St. Louis, USA c Research Center for Ageing and Osteoporosis, Copenhagen University Hospital Glostrup, Glostrup, Denmark d Osteoporosis Clinic, Dept. of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark Abstract: Background: Platelet inhibitors are widely used in the treatment and prevention of coronary artery disease. In addition to aspirin, two major groups of platelet inhibitors are used; Phosphodiesterase inhibitors including dipyridamole, and thienopyridines, to which ticlopidine and clopidogrel belong. Clopidogrel is the most widely used, and in combination with aspirin it is the standard of care for acute coronary syndromes and percutaneous coronary interventions. However, the modes of actions of these compounds involve pathways that are also involved in the metabolic activity in bone cells and pharmacologic modulation of these pathways may therefore have effects on bone turnover. Methods: In the current study, we assessed the effects of platelet inhibitors (dipyridamole and low-dose acetylsalicylic acid) on fracture incidence in a population-based epidemiological study performed within the Danish population consisting of approximately 5.3 million individuals, where all patients sustaining a fracture during the year of 2000 were included (124,655 cases). The hypotheses were to investigate if use of phosphodiesterase inhibitors and acetylsalicylic acid were associated with an increased risk of fractures after adjustment for potential confounders. Results: We found that treatment with dipyridamole is associated with increased overall fracture risk, but not to the risk of osteoporotic fractures. In contrast, low-dose ASA is associated to increased risk of both overall fractures and fractures of the hip. Conclusions: Use of oral platelet inhibitors increases the risk of fractures. When initiating treatment with oral platelet inhibitors, bone status should ideally also be assessed. This article is part of a Special Issue entitled ECTS 2011. Disclosure of interest: None declared.
doi:10.1016/j.bone.2011.03.697
PP311-M Evidence of a link between poor bone health, low vitamin D status and CVD risk in caucasian and asian women O. Hakim a, ⁎, F. Shojaee-Moradie b, K. Hart a, J. Berry c, R. Eastell d, F. Gossiel d, R. Hannon d, M. Umpleby b, B. Griffin a, S. Lanham-New a a Nutritional Sciences Division, University of Surrey, Guildford, UK b Postgraduate Medical School, University of Surrey, Guildford, UK c Department of Medicine, University of Manchester, Manchester, UK d Department of Human Metabolism, University of Sheffield, Sheffield, UK Abstract: Osteoporosis has been linked to CVD risk, suggesting that there may be an association between a poor serum lipid profile and increased bone resorption/lower bone mass. There are also data linking vitamin D ‘insufficiency’ with increased CVD. The aim of this study was to examine the association between bone health, low vitamin D status and CVD lipid profiles. The D-FINES study (Vitamin D, Food Intake, Nutrition and Exposure to Sunlight in Southern England) investigated the interaction between diet and sunlight exposure on vitamin D status. Fasted blood sample were collected at three monthly intervals from Summer 2006 to Spring 2007 on a total of 223 Caucasian (C) and 70 Asian (A) women aged 19–70 years living in Southern England. Serum CTx was assessed and parathyroid hormone (PTH) was measured. Bone quality including broadband ultrasound attenuation (BUA), velocity of sound (VOS), lumbar spine (LS) BMD, and femoral neck (FN) BMD in Autumn 2006 and Spring of 2007 were assessed. The aim of the present subsidiary study was to examine the association between bone health (bone quality, bone resorption and PTH), vitamin D status, and CVD. Correlation analysis between serum lipid profiles (serum triglyceride (TAG), total cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL)) and bone health parameters and vitamin D status have been measured.