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Introduction to fluorescence spectroscopy
Microchemical Journal 65 Ž2000. 353
Book review
Introduction to fluorescence spectroscopy John Wiley, 605 Third Avenue, New York 10158, USA, 1999;173 pp., ISBN 0-471-11098-1 This volume provides an overview of the instrumentation, sample preparation, and measurement procedures of fluorescence spectroscopy. The first two chapters consider the theory of molecular fluorescence, including the significance of the molecular electronic structure, absorption of light, non-radiative relaxation mechanisms, and radiative relaxation mechanisms. The effects of solvent and pH upon fluorescence are discussed, along with quenching phenomena. The third chapter considers instrumentation for fluorescence. The various instrument components are described, as well as a discussion of excitation and emission spectra. A discussion of all aspects of time-resolved and phase-resolved fluorescence is the topic of Chapter 4. The principles, instrumentation, and methodology of these techniques are described for the determination of fluorescence lifetime measurements. The final chapter of the volume considers applications of fluorescence. A
wide variety of techniques are discussed, including direct methods, quenching-based methods, immunoassays, and sensors. The conclusion of the chapter considers the future of fluorescence spectroscopy. In general, this volume is an excellent but brief introduction to fluorescence spectroscopy. It discusses all of the most important recent instrumental developments, while describing their applicability to analytical problems. One drawback of the volume is that there are no literature citations in the text; instead a list of suggested reading is provided at the conclusion of each chapter. This makes it difficult for the reader to find references to specific topics discussed in the book. However, I believe it does a good job of surveying the major developments in molecular fluorescence analysis. David J. Butcher Department of Chemistry and Physics, Western Carolina Uni¨ ersity, Cullowhee, NC 28723, USA
0026-265Xr00r$ - see front matter 䊚 2000 Elsevier Science B.V. All rights reserved. PII: S 0 0 2 6 - 2 6 5 X Ž 0 0 . 0 0 0 4 8 - 5
Book review
Introduction to fluorescence spectroscopy John Wiley, 605 Third Avenue, New York 10158, USA, 1999;173 pp., ISBN 0-471-11098-1 This volume provides an overview of the instrumentation, sample preparation, and measurement procedures of fluorescence spectroscopy. The first two chapters consider the theory of molecular fluorescence, including the significance of the molecular electronic structure, absorption of light, non-radiative relaxation mechanisms, and radiative relaxation mechanisms. The effects of solvent and pH upon fluorescence are discussed, along with quenching phenomena. The third chapter considers instrumentation for fluorescence. The various instrument components are described, as well as a discussion of excitation and emission spectra. A discussion of all aspects of time-resolved and phase-resolved fluorescence is the topic of Chapter 4. The principles, instrumentation, and methodology of these techniques are described for the determination of fluorescence lifetime measurements. The final chapter of the volume considers applications of fluorescence. A
wide variety of techniques are discussed, including direct methods, quenching-based methods, immunoassays, and sensors. The conclusion of the chapter considers the future of fluorescence spectroscopy. In general, this volume is an excellent but brief introduction to fluorescence spectroscopy. It discusses all of the most important recent instrumental developments, while describing their applicability to analytical problems. One drawback of the volume is that there are no literature citations in the text; instead a list of suggested reading is provided at the conclusion of each chapter. This makes it difficult for the reader to find references to specific topics discussed in the book. However, I believe it does a good job of surveying the major developments in molecular fluorescence analysis. David J. Butcher Department of Chemistry and Physics, Western Carolina Uni¨ ersity, Cullowhee, NC 28723, USA
0026-265Xr00r$ - see front matter 䊚 2000 Elsevier Science B.V. All rights reserved. PII: S 0 0 2 6 - 2 6 5 X Ž 0 0 . 0 0 0 4 8 - 5