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IS HYPOADIPONECTINEMY A RISK FACTOR FOR CORONARY ARTERY DISEASE?
Poster Sessions PO41 Screening for CV risk – circulating markers PO41-633
S-ADENOSYLHOMOCYSTEINE IS A BETTER BIOMARKER OF ATHEROSCLEROTIC LESIONS THAN HOMOCYSTEINE IN APOE-DEFICIENT MICE FED HIGH DIETARY METHIONINE
W. Ling, C. Liu, M. Xia. Department of Nutrition/School of Public Health/Sun Yat-sen University
PO41-634
STUDY OF POTENTIAL BIOMARKERS AND ENOS GENE POLYMORPHISM TO EVALUATE CAD RISK IN POSTMENOPAUSAL WOMEN
J. Bhattacharjee 1 , P.K. Dabla 1 , S.S. Trivedi 2 , N. Das 3 . 1 Department of Biochemistry, Lady Hardinge Medical College and Associated Hospitals, New Delhi, Delhi, India; 2 Department of Obs. & Gyne., Lady Hardinge Medical College and Associated Hospitals, New Delhi, Delhi, India; 3 Department of Biochemistry, AIIMS, New Delhi, India Background: The coronary artery disease (CAD) risk for women increases significantly after menopause. Glu298Asp polymorphism of eNOS is associated with increase CAD risk. The study was aimed to determine CAD risk through Glu298Asp polymorphism and interrelationship of different biomarkers. Methods: The study design consisted of 50 cases of postmenopausal women and 50 age matched premenopausal controls. We investigated the interrelationship among Plasma NO levels with Plasma Estrogen, Platelet Factor-4 (PF4) & Malonaldehyde (MDA) levels. Glu298Asp polymorphism of eNOS was studied and correlated with other biomarkers. Result: The study group showed significant decrease in plasma NO and estrogen levels. Plasma NO showed significant positive correlation (P<0.05) with plasma estrogen level in both study & control groups while significant inverse relation (P<0.05) is found in between plasma NO & PF4. For the eNOS gene, GG genotype was found in 41 cases and 45 controls while GT genotype was seen in 9 cases and 5 controls. No TT genotype was found. Significant decrease was observed in NO levels of GG+GT (n=50), GG (n=41), GT (n=9) genotypes of study group as compared to GG+GT (n=50), GG (n=45), GT (n=5) genotypes of control group. Conclusion: The correlation of NO levels with other biomarkers may play a major role in evaluations of endothelial dysfunction in Postmenopausal women. Decreased NO levels was found in cases as compared to controls,in all the genotypes of eNOS Glu298Asp gene polymorphism. These biomarkers and their interrelationship might improve early intervention and therapeutic strategies of CAD risk in postmenopausal women.
IS HYPOADIPONECTINEMY A RISK FACTOR FOR CORONARY ARTERY DISEASE?
U. Ozturk 1 , I. Karaca 1 , M. Yavuzkir 1 , N. Dagli 1 , M. Balin 3 , V. Polat 1 , H. Akbulut 2 . 1 Cardiology Department, Firat University, Firat Medical Center, Elazig, Turkey; 2 Immunology Department, Firat University, Firat Medical Center, Elazig, Turkey; 3 Cardiology Department, Elazig Government Hospital, Elazig, Turkey Background and aim: This infers that the classification of risk factors based on current risk factors is insufficient and other risk factors different from the known ones also play role in atherosclerosis process. Adiponectin is known to have protective effects in the beginning and progression of atherosclerosis due to its anti-inflammatory and anti-atherogenic effects. The aim of this study was to assess whether hypoadiponectinemy may be a risk factor for CAD. Methods: 30 patients were CAD (group 1) and 30 patients formed control group (group 2). Adiponectin levels were worked by Human Adiponectin Sandwich ELISA Kit. Results: Serum adiponectin levels were 3.30+1.96µg/d L in group 1 and 6.73+4.0µg/dL in group 2 (p<0.001). Backward regression analysis estimated CAD in meaningful level (χ2=15.329, df=1, p=0.001, R2=0.229). According to the analysis adiponectin was found as an effective factor in prediction of the existence of CAD [β=0.725, p=0.001, 95% CI (0.6040.870)]. True classification power of this logistic regression model at the last step was 77.3%. Discussion: The results encourage that adiponectin plasma level can be a valuable cytokine in predicting CAD since we found adiponectin plasma level low in CAD. Therefore hypoadiponectinemy could be a risk factor for CAD. We also found that a unit drop in adiponectin plasma level increased CAD possibility 0.229 times. Hypoadiponectinemy indicates not a possible risk but a current risk. We think that adiponectin level in CAD should be worked on larger groups for cost/benefit regarding atherosclerosis risk factors to be able to use as a routine marker in diagnosing CAD. PO41-636
NMR-DETERMINED LIPOPROTEIN SUBPOPULATION DISTRIBUTION REVEALS A HIGHER RISK FOR CVD ASSOCIATED WITH THE METABOLIC SYNDROME
H. Barakat 1 , D. Holbert 2 . 1 Dept. Internal Medicine, ECU, Greenville, NC, 27834, USA; 2 Dept. Biostatistics, ECU, Greenville, NC, 27834, USA Small and dense LDL and large VLDL particles are prevalent in patients with CVD and patients with type 2 diabetes in whom CVD is the leading cause of death. Recently emphasis has been placed on the risk for CVD in subjects with the metabolic syndrome (metsyn), but little is known about the subpopulation distribution of the plasma lipoproteins in these subjects. Methods: NMR was used to determine (1) the average particle size of each plasma lipoprotein class; (2) the relative abundance of the subclasses of each of the lipoproteins in women without (N=24), and with metsyn (N=14). Subjects with metsyn had at least 3 of the 5 characteristics of metsyn as defined by the American Heart Association (triglycerides >150 mg/dl, glucose > 125 mg/dl & HDL-C < 40 mg/dl). Results: In the subjects with metsyn, we observed (1) Age-adjusted mean plasma VLDL TG and VLDL average particle size were significantly greater (P<0.05). (2) The average diameter of both HDL and LDL particles was smaller. (3) A higher abundance (P<0.05) of small and dense LDL and large, more buoyant VLDL subclasses. Using subpopulation abundance measures to identify an optimal linear discriminant function showed that the abundance of BOTH small LDL and large VLDL particles clearly differentiated those with and without metsyn. These patterns of the type and abundance of the lipoprotein subpopulation particles are characteristic of subjects with CVD. Conclusion: The metabolic syndrome imparts a higher CVD risk in women irrespective of age. Prospective studies are warranted to verify this conclusion. PO41-637
LIPID PROFILE AND HYPERHOMOCYTEINEMIA IN THE ACTIVE STAGE OF BEHÇET’ S DISEASE
S. Hammami 1 , A. Barhoumi 1 , N. Kaoubaa 2 , A. Hamzaoui 1 , A. Nakbi 2 , M. Hammami 2 , S. Mahjoub 1 . 1 Department of Internal medicine, CHU F. Bourguiba, Monastir,Tunisia; 2 Laboratory of Biochemistry, UR Background and aims: Behçet’s disease (BD) is an inflammatory disorder
77th Congress of the European Atherosclerosis Society, April 26–29, 2008, Istanbul, Turkey
POSTER SESSIONS
Background and aims: Plasma homocysteine (Hcy) and Sadenosylhomocysteine (AdoHcy) are critical intermediates of methionine metabolism. In the present study, methionine and vitamin dietary intervention were used to induce Hcy and AdoHcy in an attempt to identify which one is closely associated with atherosclerotic lesion in apolipoprotein (apo) E-deficient mice. Methods: Eight-week-old apoE-deficient mice were divided into five groups: AIN-93G diet as a control (C), the same diet supplemented with methionine (M), the diet rich in methionine combined with deficiency in folate, Vitamin B-6, and B-12 (B vitamin) (M-V), the diet rich in methionine and B vitamin (M+V), or the diet deficient in B vitamin alone (C-V). Results: Eight weeks later, plasma homocysteine (tHcy), AdoHcy, atherosclerotic plaque area in the aortic sinus, DNA methyltransferase (Dnmt) activity, and global DNA methylation in the aortae were assayed. The increased atherosclerotic lesions were observed in mice with higher AdoHcy levels, but with normal range of plasma tHcy levels in the M+V group. However, no increment of the atheromatous lesions was found in mice with normal plasma AdoHcy, even moderate elevation of plasma tHcy in the C-V group. A negative correlation was observed between plasma AdoHcy and Dnmt activity, and global DNA methylation status. Conclusions: the present study showed that methionine enrichment induced-atherogenic effects in apoE-deficient mice might be relevant to an AdoHcy-mediated inhibition on DNA methylation atherosclerosis, and suggested that AdoHcy is a better biomaker of atherosclerotic lesions than Hcy.
PO41-635
173
S-ADENOSYLHOMOCYSTEINE IS A BETTER BIOMARKER OF ATHEROSCLEROTIC LESIONS THAN HOMOCYSTEINE IN APOE-DEFICIENT MICE FED HIGH DIETARY METHIONINE
W. Ling, C. Liu, M. Xia. Department of Nutrition/School of Public Health/Sun Yat-sen University
PO41-634
STUDY OF POTENTIAL BIOMARKERS AND ENOS GENE POLYMORPHISM TO EVALUATE CAD RISK IN POSTMENOPAUSAL WOMEN
J. Bhattacharjee 1 , P.K. Dabla 1 , S.S. Trivedi 2 , N. Das 3 . 1 Department of Biochemistry, Lady Hardinge Medical College and Associated Hospitals, New Delhi, Delhi, India; 2 Department of Obs. & Gyne., Lady Hardinge Medical College and Associated Hospitals, New Delhi, Delhi, India; 3 Department of Biochemistry, AIIMS, New Delhi, India Background: The coronary artery disease (CAD) risk for women increases significantly after menopause. Glu298Asp polymorphism of eNOS is associated with increase CAD risk. The study was aimed to determine CAD risk through Glu298Asp polymorphism and interrelationship of different biomarkers. Methods: The study design consisted of 50 cases of postmenopausal women and 50 age matched premenopausal controls. We investigated the interrelationship among Plasma NO levels with Plasma Estrogen, Platelet Factor-4 (PF4) & Malonaldehyde (MDA) levels. Glu298Asp polymorphism of eNOS was studied and correlated with other biomarkers. Result: The study group showed significant decrease in plasma NO and estrogen levels. Plasma NO showed significant positive correlation (P<0.05) with plasma estrogen level in both study & control groups while significant inverse relation (P<0.05) is found in between plasma NO & PF4. For the eNOS gene, GG genotype was found in 41 cases and 45 controls while GT genotype was seen in 9 cases and 5 controls. No TT genotype was found. Significant decrease was observed in NO levels of GG+GT (n=50), GG (n=41), GT (n=9) genotypes of study group as compared to GG+GT (n=50), GG (n=45), GT (n=5) genotypes of control group. Conclusion: The correlation of NO levels with other biomarkers may play a major role in evaluations of endothelial dysfunction in Postmenopausal women. Decreased NO levels was found in cases as compared to controls,in all the genotypes of eNOS Glu298Asp gene polymorphism. These biomarkers and their interrelationship might improve early intervention and therapeutic strategies of CAD risk in postmenopausal women.
IS HYPOADIPONECTINEMY A RISK FACTOR FOR CORONARY ARTERY DISEASE?
U. Ozturk 1 , I. Karaca 1 , M. Yavuzkir 1 , N. Dagli 1 , M. Balin 3 , V. Polat 1 , H. Akbulut 2 . 1 Cardiology Department, Firat University, Firat Medical Center, Elazig, Turkey; 2 Immunology Department, Firat University, Firat Medical Center, Elazig, Turkey; 3 Cardiology Department, Elazig Government Hospital, Elazig, Turkey Background and aim: This infers that the classification of risk factors based on current risk factors is insufficient and other risk factors different from the known ones also play role in atherosclerosis process. Adiponectin is known to have protective effects in the beginning and progression of atherosclerosis due to its anti-inflammatory and anti-atherogenic effects. The aim of this study was to assess whether hypoadiponectinemy may be a risk factor for CAD. Methods: 30 patients were CAD (group 1) and 30 patients formed control group (group 2). Adiponectin levels were worked by Human Adiponectin Sandwich ELISA Kit. Results: Serum adiponectin levels were 3.30+1.96µg/d L in group 1 and 6.73+4.0µg/dL in group 2 (p<0.001). Backward regression analysis estimated CAD in meaningful level (χ2=15.329, df=1, p=0.001, R2=0.229). According to the analysis adiponectin was found as an effective factor in prediction of the existence of CAD [β=0.725, p=0.001, 95% CI (0.6040.870)]. True classification power of this logistic regression model at the last step was 77.3%. Discussion: The results encourage that adiponectin plasma level can be a valuable cytokine in predicting CAD since we found adiponectin plasma level low in CAD. Therefore hypoadiponectinemy could be a risk factor for CAD. We also found that a unit drop in adiponectin plasma level increased CAD possibility 0.229 times. Hypoadiponectinemy indicates not a possible risk but a current risk. We think that adiponectin level in CAD should be worked on larger groups for cost/benefit regarding atherosclerosis risk factors to be able to use as a routine marker in diagnosing CAD. PO41-636
NMR-DETERMINED LIPOPROTEIN SUBPOPULATION DISTRIBUTION REVEALS A HIGHER RISK FOR CVD ASSOCIATED WITH THE METABOLIC SYNDROME
H. Barakat 1 , D. Holbert 2 . 1 Dept. Internal Medicine, ECU, Greenville, NC, 27834, USA; 2 Dept. Biostatistics, ECU, Greenville, NC, 27834, USA Small and dense LDL and large VLDL particles are prevalent in patients with CVD and patients with type 2 diabetes in whom CVD is the leading cause of death. Recently emphasis has been placed on the risk for CVD in subjects with the metabolic syndrome (metsyn), but little is known about the subpopulation distribution of the plasma lipoproteins in these subjects. Methods: NMR was used to determine (1) the average particle size of each plasma lipoprotein class; (2) the relative abundance of the subclasses of each of the lipoproteins in women without (N=24), and with metsyn (N=14). Subjects with metsyn had at least 3 of the 5 characteristics of metsyn as defined by the American Heart Association (triglycerides >150 mg/dl, glucose > 125 mg/dl & HDL-C < 40 mg/dl). Results: In the subjects with metsyn, we observed (1) Age-adjusted mean plasma VLDL TG and VLDL average particle size were significantly greater (P<0.05). (2) The average diameter of both HDL and LDL particles was smaller. (3) A higher abundance (P<0.05) of small and dense LDL and large, more buoyant VLDL subclasses. Using subpopulation abundance measures to identify an optimal linear discriminant function showed that the abundance of BOTH small LDL and large VLDL particles clearly differentiated those with and without metsyn. These patterns of the type and abundance of the lipoprotein subpopulation particles are characteristic of subjects with CVD. Conclusion: The metabolic syndrome imparts a higher CVD risk in women irrespective of age. Prospective studies are warranted to verify this conclusion. PO41-637
LIPID PROFILE AND HYPERHOMOCYTEINEMIA IN THE ACTIVE STAGE OF BEHÇET’ S DISEASE
S. Hammami 1 , A. Barhoumi 1 , N. Kaoubaa 2 , A. Hamzaoui 1 , A. Nakbi 2 , M. Hammami 2 , S. Mahjoub 1 . 1 Department of Internal medicine, CHU F. Bourguiba, Monastir,Tunisia; 2 Laboratory of Biochemistry, UR Background and aims: Behçet’s disease (BD) is an inflammatory disorder
77th Congress of the European Atherosclerosis Society, April 26–29, 2008, Istanbul, Turkey
POSTER SESSIONS
Background and aims: Plasma homocysteine (Hcy) and Sadenosylhomocysteine (AdoHcy) are critical intermediates of methionine metabolism. In the present study, methionine and vitamin dietary intervention were used to induce Hcy and AdoHcy in an attempt to identify which one is closely associated with atherosclerotic lesion in apolipoprotein (apo) E-deficient mice. Methods: Eight-week-old apoE-deficient mice were divided into five groups: AIN-93G diet as a control (C), the same diet supplemented with methionine (M), the diet rich in methionine combined with deficiency in folate, Vitamin B-6, and B-12 (B vitamin) (M-V), the diet rich in methionine and B vitamin (M+V), or the diet deficient in B vitamin alone (C-V). Results: Eight weeks later, plasma homocysteine (tHcy), AdoHcy, atherosclerotic plaque area in the aortic sinus, DNA methyltransferase (Dnmt) activity, and global DNA methylation in the aortae were assayed. The increased atherosclerotic lesions were observed in mice with higher AdoHcy levels, but with normal range of plasma tHcy levels in the M+V group. However, no increment of the atheromatous lesions was found in mice with normal plasma AdoHcy, even moderate elevation of plasma tHcy in the C-V group. A negative correlation was observed between plasma AdoHcy and Dnmt activity, and global DNA methylation status. Conclusions: the present study showed that methionine enrichment induced-atherogenic effects in apoE-deficient mice might be relevant to an AdoHcy-mediated inhibition on DNA methylation atherosclerosis, and suggested that AdoHcy is a better biomaker of atherosclerotic lesions than Hcy.
PO41-635
173