- Email: [email protected]
L 037 EFFECT OF IMMUNIZATION WITH oxLDL AND ONE PEPTIDE DERIVATED FROM oxLDL IN EVOLUTION OF ATHEROSCLEROSIS
Results: Total cholesterol in Control group (180.53 ± 19.58 mg/dL) was lower than the H (243.48 ± 29.68 mg/dL) and HA (221.03 ± 19.62 mg/dL) groups. There was no significant difference of physical activity levels and CC between groups. However, independently of group, we observed significant and negative correlation between AFL with LDL-c (r= – 0.28; p= 0.01), significant and positive correlations between EFL and AFT with HDL-c (r= 0.24; p= 0.04 and r= 0.26; p= 0.02, respectively) and AFL with CC (r= – 0.26; p= 0.02). We verified significant and positive correlation between CC with VLDL-c (r=0.26 and p=0.02) and significant negative correlation between CC with HDL-c (r= – 0.29 and p=0.01). Conclusions: The results demonstrated that independently of clinic profile, CC and activity physical level represent important parameters in prevention and treatment of atherosclerosis.
0.0016) than in CG (median 0.76). The percentage of activated platelet was equally higher in G1 (median 2.72; p= 0.0029), G 2 (median 2.47;p= 0.0002) and G 3 (median 1.98) than in CG (0.97). There was not difference among patient groups. When the degree of activation of RP was evaluated it was not observed differences between CG and dyslipidemic patients. An increased number of larger platelets, RP and activated platelets in patients with abnormalities in lipid profile suggest the excess of lipoproteins may promote cell activation and platelet hyperaggregability leading to a higher risk for atherothrombotic events.
L 035
INVOLVEMENT OF OXIDATIVE STRESS IN ATHEROSCLEROSIS: EXPERIMENTAL STUDY WITH Agaricus sylvaticus AND ANTIOXIDANT VITAMINS SUPPLEMENTATION
L 037
Sandro Percário, Valdir Francisco Odorizzi, Doroteia Rossi Silva Souza, Wataro Nelson Ogowa
EFFECT OF IMMUNIZATION WITH oxLDL AND ONE PEPTIDE DERIVATED FROM oxLDL IN EVOLUTION OF ATHEROSCLEROSIS
São José do Rio Preto Medical School São José do Rio Preto SP BRASIL e University Federal of Pará Belém PA BRASIL
Tonini, G C, Dorighello, G G E, Oliveira, H C F, Juliano, M A, Gidlund, M A
Objective: verify the potential benefits of a diet rich with natural antioxidants for the prevention of atheroma plaques onset in rabbit model. Methods: 27- New Zealand male rabbits – diet rich in cholesterol for ten weeks; 10- rabbits supplementation with mushroom Agaricus sylvaticus (Mushroom Group); while others (N=6) – vitamin C and E supplementation (Vitamins Group); the remaining animals (N=11) – Cholesterol Group. Control Group: 10 animals. After atherosclerosis induction, blood samples were drawn for total cholesterol (TC) and oxidative stress markers: thiobarbituric acid reactive substances (TBARS), uric acid and total antioxidant status (TAS). The aorta artery was removed for the evaluation of the atheroma plaques by means of computerized planimetry (PLAQUES). Results: Table 1 presents values of the analyzed parameters. High TC levels were observed in all groups, except Control. The TBARS was found higher in Vitamins and Cholesterol groups, and statistically equal in Mushroom and Control groups. High uric acid levels found in animals of Cholesterol Group suggest the involvement of ischemia-reperfusion syndrome, a well-known free radical production condition. Conclusion: These findings suggest that, oxidative processes are implicated in the genesis of the disease, and that an antioxidant dietary supplementation might prevent it.
Instituto de Ciências Biomédicas – USP São Paulo SP BRASIL e Faculdade de Biologia – UNICAMP Campinas SP BRASIL
L 036
Atherosclerosis is a chronic inflammatory disease responsible for more than 40% of the deaths in industrial countries. The presence of oxidized LDL (oxLDL) and autoantibodies against oxLDL are implicated as important predictors of disease severity. Our aims were to analyze the effects of immunization with oxLDL and a synthetic apoB derived peptide (pepD) in evolution of atherosclerosis.Animals LDLr-/- were immunized with oxLDL (oxLDL group) and an pepD (pepD group). In the serum of this animals were determined the presence of antibodies anti-oxLDL and anti-pepD by ELISA (results were expressed in relation to IgG control) and total cholesterol and triacylglycerols (TG) by enzymatic methods. The lipid-stained lesions were quantified using Image Pro Plus software for image analysis (Media Cybernetics, Silver Spring, MD). The area of the lesions was expressed as the sum of the lesions in 6 cross-sections of 10ìm, 80ìm distant from each other in a total aorta length of 480ìm. The oxLDL group showed higher plasma levels of TG ([2.7±0.9] 102 mg/dL) as well as higher antibodies titers anti-oxLDL (4.3±0.2 relative absorbance) as compared to the pepD group ([1.6±0.5] 102 mg/dL and 0.9±0.1 relative absorbance, respectively) and the control group ([1.8±0.7] 102 mg/dL and 1.0±0.1 relative absorbance, respectively). However, the level of antibodies titers anti-pepD in pepD group was lower (0.9±0.2 relative absorbance) than in the other groups (control: 1.5±0.8 and oxLDL group: 1.6±0.4).The oxLDL group showed a diminution of 54.1% of the atherosclerotic lesion size whilst the pepD group showed a diminution of 41%. Although in the oxLDL group the level of TG (molecules considered pro-atherogenic) was increased, there was a diminution of the atherosclerotic lesion size. This further strengthen a possible protector role of antibodies against oxLDL. Most interestingly, pepD is now evaluated as a candidate for developing a new vaccine against atherosclerosis as it partly prevented the development of atherosclerotic lesion size without an increase of the level of TG.
EVALUATION OF ANTHROPOMETRIC MEASURES AND PHYSICAL ACTIVITY LEVEL IN SUBJECT WITH DIFFERENT LEVELS OF CARDIOVASCULAR RISK
Sanibal, C A, Mello, A P Q, Timm, A S, Damasceno, N R T Faculdade de Saúde Publica São Paulo SP BRASIL Introduction: There is a strong link between abdominal fat and physical activity level on cardiovascular heart diseases and, particularly, in atherosclerosis. Objective: Evaluated the physical activity level and waist circumference of subjects with different levels of cardiovascular risks and to establish their correlation with lipid profile. Methods: Seventy tree individuals the both gender were selected from INCOR and University Hospital – HU/USP (Sao Paulo, Brazil). They were divided in Control group (n= 30), Hypercholesterolemic group –H (n= 33) and Hypercholesterolemic with atherosclerosis group – HA (n= 10). After 12 h of fast, blood samples were collected and from plasma, lipid profiles were analyzed (colorimetric and enzymatic reactions). The habitual physical activity formed by the total physical activity – AFT, occupational physical activity – AFO, leisure and locomotion physical activity – AFL and practised physical exercises during the leisure time – EFL, were monitored according Baecke et. al. (1982). The waist circumference (CC) was evaluated according with International Diabetes Federation – IDF. Statistical analysis was performed by SPSS program, version 15.0.
L 038
ATHEROSCLEROTIC LESIONS EXPRESS MRNA FOR IL-17, IL-17R, IL-23 AND IL-23R
Rômulo Tadeu Dias de Oliveira, Ronei Luciano Mamoni, Maria Carolina Ferreira, Larissa Nara Alegrini Longui, José Roberto Matos Souza, Juliano de Lara Fernandes, Fábio Husemman Menezes, Maria Heloísa Blotta Universidade de Campinas Campinas SP BRASIL Atherosclerosis is an inflammatory disease characterized by an infiltration of macrophages, T lymphocytes and inflammatory mediators, suggesting that a cellular immune response may take place in the lesions contributing to plaque growth and disruption. Recently, a new subtype of IL-17-producing T helper (TH-17) lineage of T cells has been described and associated to a variety of inflammatory autoimmune diseases. The aim of this study was to assess whether 26
0.0016) than in CG (median 0.76). The percentage of activated platelet was equally higher in G1 (median 2.72; p= 0.0029), G 2 (median 2.47;p= 0.0002) and G 3 (median 1.98) than in CG (0.97). There was not difference among patient groups. When the degree of activation of RP was evaluated it was not observed differences between CG and dyslipidemic patients. An increased number of larger platelets, RP and activated platelets in patients with abnormalities in lipid profile suggest the excess of lipoproteins may promote cell activation and platelet hyperaggregability leading to a higher risk for atherothrombotic events.
L 035
INVOLVEMENT OF OXIDATIVE STRESS IN ATHEROSCLEROSIS: EXPERIMENTAL STUDY WITH Agaricus sylvaticus AND ANTIOXIDANT VITAMINS SUPPLEMENTATION
L 037
Sandro Percário, Valdir Francisco Odorizzi, Doroteia Rossi Silva Souza, Wataro Nelson Ogowa
EFFECT OF IMMUNIZATION WITH oxLDL AND ONE PEPTIDE DERIVATED FROM oxLDL IN EVOLUTION OF ATHEROSCLEROSIS
São José do Rio Preto Medical School São José do Rio Preto SP BRASIL e University Federal of Pará Belém PA BRASIL
Tonini, G C, Dorighello, G G E, Oliveira, H C F, Juliano, M A, Gidlund, M A
Objective: verify the potential benefits of a diet rich with natural antioxidants for the prevention of atheroma plaques onset in rabbit model. Methods: 27- New Zealand male rabbits – diet rich in cholesterol for ten weeks; 10- rabbits supplementation with mushroom Agaricus sylvaticus (Mushroom Group); while others (N=6) – vitamin C and E supplementation (Vitamins Group); the remaining animals (N=11) – Cholesterol Group. Control Group: 10 animals. After atherosclerosis induction, blood samples were drawn for total cholesterol (TC) and oxidative stress markers: thiobarbituric acid reactive substances (TBARS), uric acid and total antioxidant status (TAS). The aorta artery was removed for the evaluation of the atheroma plaques by means of computerized planimetry (PLAQUES). Results: Table 1 presents values of the analyzed parameters. High TC levels were observed in all groups, except Control. The TBARS was found higher in Vitamins and Cholesterol groups, and statistically equal in Mushroom and Control groups. High uric acid levels found in animals of Cholesterol Group suggest the involvement of ischemia-reperfusion syndrome, a well-known free radical production condition. Conclusion: These findings suggest that, oxidative processes are implicated in the genesis of the disease, and that an antioxidant dietary supplementation might prevent it.
Instituto de Ciências Biomédicas – USP São Paulo SP BRASIL e Faculdade de Biologia – UNICAMP Campinas SP BRASIL
L 036
Atherosclerosis is a chronic inflammatory disease responsible for more than 40% of the deaths in industrial countries. The presence of oxidized LDL (oxLDL) and autoantibodies against oxLDL are implicated as important predictors of disease severity. Our aims were to analyze the effects of immunization with oxLDL and a synthetic apoB derived peptide (pepD) in evolution of atherosclerosis.Animals LDLr-/- were immunized with oxLDL (oxLDL group) and an pepD (pepD group). In the serum of this animals were determined the presence of antibodies anti-oxLDL and anti-pepD by ELISA (results were expressed in relation to IgG control) and total cholesterol and triacylglycerols (TG) by enzymatic methods. The lipid-stained lesions were quantified using Image Pro Plus software for image analysis (Media Cybernetics, Silver Spring, MD). The area of the lesions was expressed as the sum of the lesions in 6 cross-sections of 10ìm, 80ìm distant from each other in a total aorta length of 480ìm. The oxLDL group showed higher plasma levels of TG ([2.7±0.9] 102 mg/dL) as well as higher antibodies titers anti-oxLDL (4.3±0.2 relative absorbance) as compared to the pepD group ([1.6±0.5] 102 mg/dL and 0.9±0.1 relative absorbance, respectively) and the control group ([1.8±0.7] 102 mg/dL and 1.0±0.1 relative absorbance, respectively). However, the level of antibodies titers anti-pepD in pepD group was lower (0.9±0.2 relative absorbance) than in the other groups (control: 1.5±0.8 and oxLDL group: 1.6±0.4).The oxLDL group showed a diminution of 54.1% of the atherosclerotic lesion size whilst the pepD group showed a diminution of 41%. Although in the oxLDL group the level of TG (molecules considered pro-atherogenic) was increased, there was a diminution of the atherosclerotic lesion size. This further strengthen a possible protector role of antibodies against oxLDL. Most interestingly, pepD is now evaluated as a candidate for developing a new vaccine against atherosclerosis as it partly prevented the development of atherosclerotic lesion size without an increase of the level of TG.
EVALUATION OF ANTHROPOMETRIC MEASURES AND PHYSICAL ACTIVITY LEVEL IN SUBJECT WITH DIFFERENT LEVELS OF CARDIOVASCULAR RISK
Sanibal, C A, Mello, A P Q, Timm, A S, Damasceno, N R T Faculdade de Saúde Publica São Paulo SP BRASIL Introduction: There is a strong link between abdominal fat and physical activity level on cardiovascular heart diseases and, particularly, in atherosclerosis. Objective: Evaluated the physical activity level and waist circumference of subjects with different levels of cardiovascular risks and to establish their correlation with lipid profile. Methods: Seventy tree individuals the both gender were selected from INCOR and University Hospital – HU/USP (Sao Paulo, Brazil). They were divided in Control group (n= 30), Hypercholesterolemic group –H (n= 33) and Hypercholesterolemic with atherosclerosis group – HA (n= 10). After 12 h of fast, blood samples were collected and from plasma, lipid profiles were analyzed (colorimetric and enzymatic reactions). The habitual physical activity formed by the total physical activity – AFT, occupational physical activity – AFO, leisure and locomotion physical activity – AFL and practised physical exercises during the leisure time – EFL, were monitored according Baecke et. al. (1982). The waist circumference (CC) was evaluated according with International Diabetes Federation – IDF. Statistical analysis was performed by SPSS program, version 15.0.
L 038
ATHEROSCLEROTIC LESIONS EXPRESS MRNA FOR IL-17, IL-17R, IL-23 AND IL-23R
Rômulo Tadeu Dias de Oliveira, Ronei Luciano Mamoni, Maria Carolina Ferreira, Larissa Nara Alegrini Longui, José Roberto Matos Souza, Juliano de Lara Fernandes, Fábio Husemman Menezes, Maria Heloísa Blotta Universidade de Campinas Campinas SP BRASIL Atherosclerosis is an inflammatory disease characterized by an infiltration of macrophages, T lymphocytes and inflammatory mediators, suggesting that a cellular immune response may take place in the lesions contributing to plaque growth and disruption. Recently, a new subtype of IL-17-producing T helper (TH-17) lineage of T cells has been described and associated to a variety of inflammatory autoimmune diseases. The aim of this study was to assess whether 26