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Mass Spectrometry of Biological Materials
304
Mass Spectrometry of Biological Materials, edited by C.N. McEwen and
B.S. Larsen, Practical Spectroscopy York, 1990, 515 pp.
Series Vol. 8, Marcel Dekker, New
This book is organized in two main, well-differentiated sections. The first section, on proteins, presents an excellent overview of the different techniques available to obtain and interpret mass spectra of proteins with six chapters covering everything from historical developments to practical tips for the analysis of peptides by FAB and including: an in-depth overview (K. Biemann); protein isolation and structural analysis (J. Shively and R. Paxton); structural analysis of post-translationally modified proteins (S.A. Carr et al.); natural and recombinant proteins and glycoproteins (H.R. Morris et al.); sequencing by tandem MS (D.F. Hunt et al.); and tips for FAB MS of peptides (B.S. Larsen). Approaches to protein structural and sequence analysis relying both on multiple collision at low kinetic energy of the colliding ion on triple quadrupoles and single collision at high energies on magnetic systems are well covered by representative authors: D.F. Hunt and K. Biemann respectively. In general, all of these chapters contain useful data describing in some detail modern approaches to protein chemistry and gathering in a single volume a wealth of information on this topic. Section II includes a total of nine chapters; six of them on specific applications of monoamines, lipids, sterols, steroids and bile acids, pesticides and porphyrin sensitizers whereas the other three chapters deal with general MS techniques for biological materials such as isotope dilution, capillary GC-MS and atmospheric pressure ionization (API) LC-MS. This section follows the trend set in the first section in terms of the presentation of much useful practical information, often difficult to find in a single source, as for example in the chapters on lipids (S.T. Weintraub), steroid and bile acids (C.H. Shackleton et al.), isotope dilution (A.L. Yergey et al.), capillary GC-MS (F.G. Kitson and B.S. Larsen) and API LC-MS (J. Henion and E. Lee). In this 5 15 page volume the editors have successfully assembled an impressive group of well-known experts in various fields of biological MS and each of these authors has provided an authoritative and detailed account of experimental knowhow, recipes for proper sample handling and tips on improved methods of analysis. This is complemented by abundant, wellpresented illustrations and tables as well as by the corresponding reference lists at the end of each chapter. The book can be highly recommended, both as an essential text for newcomers to the field and as a valuable, integrated and up-to-date reference
source for anybody interested in state-of-the-art metry in biology, biochemistry and biomedicine.
research in mass spectro-
EMIL10 GELPI Department of Neurochemistry CSIC J. Girona 18-26 08034 Barcelona Spain
Mass Spectrometry of Biological Materials, edited by C.N. McEwen and
B.S. Larsen, Practical Spectroscopy York, 1990, 515 pp.
Series Vol. 8, Marcel Dekker, New
This book is organized in two main, well-differentiated sections. The first section, on proteins, presents an excellent overview of the different techniques available to obtain and interpret mass spectra of proteins with six chapters covering everything from historical developments to practical tips for the analysis of peptides by FAB and including: an in-depth overview (K. Biemann); protein isolation and structural analysis (J. Shively and R. Paxton); structural analysis of post-translationally modified proteins (S.A. Carr et al.); natural and recombinant proteins and glycoproteins (H.R. Morris et al.); sequencing by tandem MS (D.F. Hunt et al.); and tips for FAB MS of peptides (B.S. Larsen). Approaches to protein structural and sequence analysis relying both on multiple collision at low kinetic energy of the colliding ion on triple quadrupoles and single collision at high energies on magnetic systems are well covered by representative authors: D.F. Hunt and K. Biemann respectively. In general, all of these chapters contain useful data describing in some detail modern approaches to protein chemistry and gathering in a single volume a wealth of information on this topic. Section II includes a total of nine chapters; six of them on specific applications of monoamines, lipids, sterols, steroids and bile acids, pesticides and porphyrin sensitizers whereas the other three chapters deal with general MS techniques for biological materials such as isotope dilution, capillary GC-MS and atmospheric pressure ionization (API) LC-MS. This section follows the trend set in the first section in terms of the presentation of much useful practical information, often difficult to find in a single source, as for example in the chapters on lipids (S.T. Weintraub), steroid and bile acids (C.H. Shackleton et al.), isotope dilution (A.L. Yergey et al.), capillary GC-MS (F.G. Kitson and B.S. Larsen) and API LC-MS (J. Henion and E. Lee). In this 5 15 page volume the editors have successfully assembled an impressive group of well-known experts in various fields of biological MS and each of these authors has provided an authoritative and detailed account of experimental knowhow, recipes for proper sample handling and tips on improved methods of analysis. This is complemented by abundant, wellpresented illustrations and tables as well as by the corresponding reference lists at the end of each chapter. The book can be highly recommended, both as an essential text for newcomers to the field and as a valuable, integrated and up-to-date reference
source for anybody interested in state-of-the-art metry in biology, biochemistry and biomedicine.
research in mass spectro-
EMIL10 GELPI Department of Neurochemistry CSIC J. Girona 18-26 08034 Barcelona Spain