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Methods and Apparatus of Obtaining NMR Spectra
Spectrochimica Acta
NEW PATENTS This Section contains abstracts and, where appropriate, illustrations of recently issued United States patents and published patent applications filed from over 30 countries under the Patent Cooperation Treaty. This information was obtained from recent additions to the Pergamon PATSEARCH@ online database in accordance with interest profiles developed by the Editors. Further information about Pergamon PATSEARCH@ can be obtained from Pergamon InfoLine Inc., 1340 Old Chain Bridge Road, McLean, Virginia 22101 U.S.A. Copies of complete patents announced in this Section are available from Pergamon InfoLine Inc. for $8 per copy. Payment with order is required. Orders outside North America add $2 for air postage. Order by patent number for Pergamon InfoLine only.
4531094
4532217
METHODS AND APPARATUS OF OBTAINING NMR SPECTRA
BIOLOGICAL USES OF SHIFT REAGENTS FOR THE NUCLEAR MAGNETIC RESONANCE OF PHYSIOLOGICAL METAL CATIONS
Roger J Ordidge, Robert E Gordon, Nottingham, United Kingdom assigned to Oxford Research Systems Limited
Charles S Springer, Martin M Pike, James A Balschi, David M Yarmush, Simon C.K Chu assigned to The Research Foundation of State University of New York cycle
time
A method of obtaining an NMR spectrum from a sample comprising the steps of: maintaining a static magnetic field along an axis which is homogeneous over at least a small volume constituting part of a sample applying a gradient to said magnetic field which varies in one direction orthogonal to said axis while applying an rfpulse to the sample to saturate all nuclei in the said volume except for a slab lying in a plane normal to the said one direction replacing the said gradient by a second gradient to said magnetic field which varies in a second direction orthogonal to said axis and said one direction while applying an rf pulse to the sample to saturate all nuclei in the said volume except for a slab lying in a plane normal to the said second direction, whereby nuclei in a narrow column extending parallel to the said axis have their spins left undisturbed replacing the second gradient by a third gradient to said static magnetic field which varies in a direction parallel to said axis while applying an rf pulse to the sample to selectively irradiate nuclei in a small region of the said column; and finally removing the third gradient and then reading out the free induction decay signal from the selectively irradiated nuclei in the presence of the static magnetic field alone.
A novel group of nuclear magnetic resonance shift reagents is disclosed. This group comprises the anionic chelates of dysprosium and thulium wherein the chelate moiety has a charge of at least -3. These reagents cause a shift in the nuclear magnetic resonance spectrum of aqueous solutions of alkali and alkaline earth metal ions. By providing shift reagents to one side of a membrane capable of permitting the transport of alkali or alkaline earth metal ions therethrough and having such ions on one or the other side of said membrane and providing an environment in which said ions can pass through said membrane, by measuring the NMR spectrum of the total environment, the area of the peak of the absorpiton due to a predetermined ionic species caused by the shifted ions is compared to that due to the unshifted ions, the rate of transport through the membrane may be determined.
NEW PATENTS This Section contains abstracts and, where appropriate, illustrations of recently issued United States patents and published patent applications filed from over 30 countries under the Patent Cooperation Treaty. This information was obtained from recent additions to the Pergamon PATSEARCH@ online database in accordance with interest profiles developed by the Editors. Further information about Pergamon PATSEARCH@ can be obtained from Pergamon InfoLine Inc., 1340 Old Chain Bridge Road, McLean, Virginia 22101 U.S.A. Copies of complete patents announced in this Section are available from Pergamon InfoLine Inc. for $8 per copy. Payment with order is required. Orders outside North America add $2 for air postage. Order by patent number for Pergamon InfoLine only.
4531094
4532217
METHODS AND APPARATUS OF OBTAINING NMR SPECTRA
BIOLOGICAL USES OF SHIFT REAGENTS FOR THE NUCLEAR MAGNETIC RESONANCE OF PHYSIOLOGICAL METAL CATIONS
Roger J Ordidge, Robert E Gordon, Nottingham, United Kingdom assigned to Oxford Research Systems Limited
Charles S Springer, Martin M Pike, James A Balschi, David M Yarmush, Simon C.K Chu assigned to The Research Foundation of State University of New York cycle
time
A method of obtaining an NMR spectrum from a sample comprising the steps of: maintaining a static magnetic field along an axis which is homogeneous over at least a small volume constituting part of a sample applying a gradient to said magnetic field which varies in one direction orthogonal to said axis while applying an rfpulse to the sample to saturate all nuclei in the said volume except for a slab lying in a plane normal to the said one direction replacing the said gradient by a second gradient to said magnetic field which varies in a second direction orthogonal to said axis and said one direction while applying an rf pulse to the sample to saturate all nuclei in the said volume except for a slab lying in a plane normal to the said second direction, whereby nuclei in a narrow column extending parallel to the said axis have their spins left undisturbed replacing the second gradient by a third gradient to said static magnetic field which varies in a direction parallel to said axis while applying an rf pulse to the sample to selectively irradiate nuclei in a small region of the said column; and finally removing the third gradient and then reading out the free induction decay signal from the selectively irradiated nuclei in the presence of the static magnetic field alone.
A novel group of nuclear magnetic resonance shift reagents is disclosed. This group comprises the anionic chelates of dysprosium and thulium wherein the chelate moiety has a charge of at least -3. These reagents cause a shift in the nuclear magnetic resonance spectrum of aqueous solutions of alkali and alkaline earth metal ions. By providing shift reagents to one side of a membrane capable of permitting the transport of alkali or alkaline earth metal ions therethrough and having such ions on one or the other side of said membrane and providing an environment in which said ions can pass through said membrane, by measuring the NMR spectrum of the total environment, the area of the peak of the absorpiton due to a predetermined ionic species caused by the shifted ions is compared to that due to the unshifted ions, the rate of transport through the membrane may be determined.