Molecular genetics in anisakid nematodes

Symposium (E) I Parasitology

h-1:2-5

PCR-RFLP

FOR

NEMATODES

‘Kinpei

Yagi,

‘Tohru

THE

Ohyama,

Institute

of Public Health

“Sappao

Medical

University

“‘Sapporo

Medical

Anisakidosis

these

performed enabling the

in

For

are

carefully

Takahashi,

Department

School

of

is

removed

mainly

Ishikura

of Clinical

Pathology

Department

of

different,

therefore,

to clarify

by

Anisakis simplex

the

their

are

identification

epidemiological

often

GENETICS

University

IN

of Rome

ANISAKID

“La Sapienza”,

The aim of the presentation is to elucidate recent studies carried out with the extensive use of genetic markers as operational species descriptors. lsozyme analysis at 19-24 loci was carried out on anisakid nematodes of the genera Anisaki!. Pseudoferranova and Confracaecum from. various intermedtate/paratenic (squid, fish) andr,efimttve ( marme mammals) hosts from vartous parts of the A relevant

result

was

the

detection

of a number

of

and pathogenesity has

damaged

to

be

status therely

to be taken against these parasites.

by endoscope

and

27

MOLECULAR NEMATODES

Paggi L Instttute of Parasitology. Rome, Italy

***Hajime

characteristics

47 (Suppl.) (1998) 23-48

S-E2-6

ANISAKID

PATIENTS

Medicme,

caused

The biological

in order

and

However, difficult

to

parasite,

we

by microscopic techniques. an application

of

genomic

the second internal

these

chain

Japan

measures of control

clarified of

University

decipens

species

parasites

identify

Hospital,

OF

HUMAN

1

Pseudoterranova of

FROM

**Syuj~

lHokkaido

Pathology

IDENTIFICATION

REMOVED

International

two

parasites

reaction-based

( PCR-RFLP)

of ITS2

method

transcribed

and

Indicated

restriction

to

spacer the

fragment

identi@

these

of ribosomal

( ITS2

the

polymelase

polymrophism

technique

usefulness length

)

DNA

of

A.;iphidarum within genus Anisakis and of C.mirounga and C.radiarum within enus Conrrucaecum were also defined. Higher values of genetic d’tstance between A.schu akovi, A.zi hidarum. A.physeferis, genetically and morphologica Ply well dt.R erentiated taxa and sibling spectes of the Anisakis sim lex complex were observed ranging from DN~,= 1.30 to f. 09 and between Cmiroungu, Crad~atum and members of the C oscula~um complex ranging from !I,,,,= 0.83 to 1.35. Data on genetic variability and diversity, gene flow and genetic relationships between populations and species of the genera Anisakr.$, Psrudoterranova and C’on~racaecum so far studied arc discussed