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Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon (I Nazarenko et al, US)
mute, ladite sequence nucleotidique etant sous Ie conrrole du susdit promoteur comprenant Ie susdit operateur mute contenant deux sequences et derivanr d'un operareur contenant deux sequences repetees inversees (palindrome), cet operateur mute etant tel que l'une de ses sequences est mutee (mutation 1), tandis que l'autre est par exemple a l'etat sauvage, la transcription du gene indicateur etant reprimee par des proteines regulatrices sous forme d'heterodimeres via leur site de fixation a I'ADN, chaque monornere de ces heterodimeres erant tel qu'il reconnaisse specifiquement I'un la sequence comportant la mutation l'autre la sequence qui est a l'etat sauvage, notamrnent pour detecter des interactions entre deux proteines, ces interactions etant revelees lorsqu'il ya repression de la transcription du susdit gene indicateur, cette repression resultant de la formation d'heterodirneres, > Multiplexed active biologic array (GT Kovacs, US) Nanogenlnc N" WO 98/01758, PCT.
> Nucleic acid amplifica-
tion oligonucleotides with molecular energy tra,ns1er labels and methods based thereon (I Nazarenko et aI, US) Oncor Inc N° WO 98/02449, PCT. The present invention provides labeled nucleic acid amplification oligonucleotides, which can be linear or hairpin primers or blocking oligonucleotides. The oligonucleotides of the invention are labeled with donor and/or acceptor moieties of molecular energy transfer pairs. The moities can be fluorophores, such that fluorescent energy emitted by the donor is absorbed by the acceptor. The acceptor may be a fluorophore that fluoresces at a wavelength different from the donor moiety, or it may be a quencher. ..
> Microbial isoprene ge-
neration (W Zimmer, DE) Fraunhofer-Gesellschaft zur
Forderunq der Angewandten Forschung EV and the inventors N" WO 98/02550, PCT A micro-organism is disclosed, as well as the use of this micro-organism to generate isoprene and a process for producing the same. The isoprene synthase gene of a plant, for example the Englis oak, is identified and cloned in a micro-organism by means of a gene fragment amplified by appropriate oligonucleotides. The micro-organism that contains an isoprene synthase gene is cultivated and the isoprene released thereby is extracted from the gaseous space above the micro-organism culture by cold fractionation.
> Detection of individual
gene transcription and splicing (JB Lawrence et aI, US) University of Massachusetts N" WO 98/02576. PCT. Disclosed are in situ hybridization methods for differentially detecting an RNA and the gene from which it was transcribed, while preserving the spatial relationship of the RNA transcript and the gene. Also disclosed are in situ hybridization methods for simultaneouslv detecting two alleles of the same gene in a single cell, while differentially detecting RNA transcribed from each of the two alleles. Also disclosed are in situ hybridization methods for detecting normal and defective RNA splicing.
> Method to co-detect introduced genes and their products (LM Kunkel and E Gussoni, US) The Children's Medical Center Corp and the inventors N" WO 98/02577. PCT. Methods of detecting and simultaneously visualizing, in a host cell, the presence of a single copy of an exogenous nucleic acid, and a protein encoded by the exogenous nucleic acid, are disclosed. > Device for separating
micro objects (K Luttermann et aI, DE) Bayer AG and the inventors W WO 98/03628. PCT.
The invention concerns a device for transferring micro objects, in particular biological objects, from their present substrate to a target substrate. (... ) > High fidelity detection of nucleic acid differences by ligase detection reaction (F Barany et aI, US) Cornell Research Foundation Inc and Purdue Research Foundation N" WO 98/03673. PCT. Ligase detection reaction is utilized to distinguish minority template in the presence of an excess of normal template with a thermostable ligase. This process can be carried out with a mutant ligase, thermostable ligase, or a modified oligonucleotide probe. This procedure is particularly useful for the detection of cancerassociated mutations. It has the advantage of providing a quantitative measure of the amount or ratio of minority template.
> Method for determining lymphocyte distribution and trafficking in mammals using imaging (RH Rubin et ai, US) Massachusetts Institute of Technology and the General Hospital Corp N" WO 98/00560 PCT. Methods for determining lymphocyte distribution and trafficking in a mammal are described. Either a labeled ligand capable of interacting specifically with the lymphocytes of the mammal is administered to the mammal so that the labeled ligands interacts in uiuo with the lymphocytes, resulting in labeled lymphocytes, or the labeled ligand is contacted with the lymphocytes in vitro so that the labeled ligand interacts with the lymphocytes resulting in labeled lymphocytes, and these labeled lymphocytes are administered to the mammal. The distribution of trafficking of the labeled lymphocytes in the mammal is determined by imaging. (... ) > Method for detecting telomerase activity (M Hirose et aI, JP) Chugai Seiyaku Kabushiki Kaisha N" WO 98/00563. PCT
A method for detecting telomerase activity characterized by amplifying an oligonucleotide sequence elongated by a DNA elongation reaction with a telomerase and hybridizing the amplification product thus obtained with a probe labeled with a non-radioactive tag; a method of the detection of cancer cells; a method of the diagnosis of cancer; and a diagnostic kit to be used in the above-mentionned detection and diagnosis methods.
> Method and medium for
detecting vancomycin-resistant Enterococcus (CM Chen and S Edberg, US) Idexx Laboratories Inc N" WO 98/04674. PCT. A microbe-specific medium for detection of vancomycin-resistant Enterococci in a test sample within 24 hours and preferably within 18 hours. The testing medium provides a selective growth medium for vancomycin-resistant Enterococci and includes specific nutrient indicators which only the target microbe can significanrly metabolize and use for growth (... )
> A novel human mRNA
editing enzyme (J AuYoung et aI, US) Incyte Pharmaceuticals Inc and the inventors W WO 98/04714. PCT The present invention providesa polynucleotidewhich indenrifies and encodes a novel human mRNA editing enzymes (REE). The invention provides for genetically engineered expression vectors and host cells comprising the nucleic acid sequence encoding REE. The invention also provides for the use of substantially purified REE and its agonises, antagonists, or inhibitors in the commercial production of recombinant proteins in pharmaceutical compositions for the treatment of diseases associated for the expression of REE. (... ) Les brevets peuvent etre obtenus a 1'1 nstitut national de la propriete mdustrielle (Inpi), 26, rue de SaintPetersbourp, 75800 Paris cedex 08. ou des copies peuvent etre fournies sur simple demande. seton les tarrts en vigueur.
BIOFUTUR 178 • Mai 1998 49
> Nucleic acid amplifica-
tion oligonucleotides with molecular energy tra,ns1er labels and methods based thereon (I Nazarenko et aI, US) Oncor Inc N° WO 98/02449, PCT. The present invention provides labeled nucleic acid amplification oligonucleotides, which can be linear or hairpin primers or blocking oligonucleotides. The oligonucleotides of the invention are labeled with donor and/or acceptor moieties of molecular energy transfer pairs. The moities can be fluorophores, such that fluorescent energy emitted by the donor is absorbed by the acceptor. The acceptor may be a fluorophore that fluoresces at a wavelength different from the donor moiety, or it may be a quencher. ..
> Microbial isoprene ge-
neration (W Zimmer, DE) Fraunhofer-Gesellschaft zur
Forderunq der Angewandten Forschung EV and the inventors N" WO 98/02550, PCT A micro-organism is disclosed, as well as the use of this micro-organism to generate isoprene and a process for producing the same. The isoprene synthase gene of a plant, for example the Englis oak, is identified and cloned in a micro-organism by means of a gene fragment amplified by appropriate oligonucleotides. The micro-organism that contains an isoprene synthase gene is cultivated and the isoprene released thereby is extracted from the gaseous space above the micro-organism culture by cold fractionation.
> Detection of individual
gene transcription and splicing (JB Lawrence et aI, US) University of Massachusetts N" WO 98/02576. PCT. Disclosed are in situ hybridization methods for differentially detecting an RNA and the gene from which it was transcribed, while preserving the spatial relationship of the RNA transcript and the gene. Also disclosed are in situ hybridization methods for simultaneouslv detecting two alleles of the same gene in a single cell, while differentially detecting RNA transcribed from each of the two alleles. Also disclosed are in situ hybridization methods for detecting normal and defective RNA splicing.
> Method to co-detect introduced genes and their products (LM Kunkel and E Gussoni, US) The Children's Medical Center Corp and the inventors N" WO 98/02577. PCT. Methods of detecting and simultaneously visualizing, in a host cell, the presence of a single copy of an exogenous nucleic acid, and a protein encoded by the exogenous nucleic acid, are disclosed. > Device for separating
micro objects (K Luttermann et aI, DE) Bayer AG and the inventors W WO 98/03628. PCT.
The invention concerns a device for transferring micro objects, in particular biological objects, from their present substrate to a target substrate. (... ) > High fidelity detection of nucleic acid differences by ligase detection reaction (F Barany et aI, US) Cornell Research Foundation Inc and Purdue Research Foundation N" WO 98/03673. PCT. Ligase detection reaction is utilized to distinguish minority template in the presence of an excess of normal template with a thermostable ligase. This process can be carried out with a mutant ligase, thermostable ligase, or a modified oligonucleotide probe. This procedure is particularly useful for the detection of cancerassociated mutations. It has the advantage of providing a quantitative measure of the amount or ratio of minority template.
> Method for determining lymphocyte distribution and trafficking in mammals using imaging (RH Rubin et ai, US) Massachusetts Institute of Technology and the General Hospital Corp N" WO 98/00560 PCT. Methods for determining lymphocyte distribution and trafficking in a mammal are described. Either a labeled ligand capable of interacting specifically with the lymphocytes of the mammal is administered to the mammal so that the labeled ligands interacts in uiuo with the lymphocytes, resulting in labeled lymphocytes, or the labeled ligand is contacted with the lymphocytes in vitro so that the labeled ligand interacts with the lymphocytes resulting in labeled lymphocytes, and these labeled lymphocytes are administered to the mammal. The distribution of trafficking of the labeled lymphocytes in the mammal is determined by imaging. (... ) > Method for detecting telomerase activity (M Hirose et aI, JP) Chugai Seiyaku Kabushiki Kaisha N" WO 98/00563. PCT
A method for detecting telomerase activity characterized by amplifying an oligonucleotide sequence elongated by a DNA elongation reaction with a telomerase and hybridizing the amplification product thus obtained with a probe labeled with a non-radioactive tag; a method of the detection of cancer cells; a method of the diagnosis of cancer; and a diagnostic kit to be used in the above-mentionned detection and diagnosis methods.
> Method and medium for
detecting vancomycin-resistant Enterococcus (CM Chen and S Edberg, US) Idexx Laboratories Inc N" WO 98/04674. PCT. A microbe-specific medium for detection of vancomycin-resistant Enterococci in a test sample within 24 hours and preferably within 18 hours. The testing medium provides a selective growth medium for vancomycin-resistant Enterococci and includes specific nutrient indicators which only the target microbe can significanrly metabolize and use for growth (... )
> A novel human mRNA
editing enzyme (J AuYoung et aI, US) Incyte Pharmaceuticals Inc and the inventors W WO 98/04714. PCT The present invention providesa polynucleotidewhich indenrifies and encodes a novel human mRNA editing enzymes (REE). The invention provides for genetically engineered expression vectors and host cells comprising the nucleic acid sequence encoding REE. The invention also provides for the use of substantially purified REE and its agonises, antagonists, or inhibitors in the commercial production of recombinant proteins in pharmaceutical compositions for the treatment of diseases associated for the expression of REE. (... ) Les brevets peuvent etre obtenus a 1'1 nstitut national de la propriete mdustrielle (Inpi), 26, rue de SaintPetersbourp, 75800 Paris cedex 08. ou des copies peuvent etre fournies sur simple demande. seton les tarrts en vigueur.
BIOFUTUR 178 • Mai 1998 49