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O195. The prevalence of human papillomavirus (HPV) in oral and oropharynx squamous cell carcinoma
HNSCCs: HPV infection was most prevalent in oropharyngeal cancers (OPCs) (5/9, 56%), rarely found in oral cavity cancers (2/51, 4%), and not detected in tumors from other head and neck sites (0/11, 0%). The high-risk HPV-16 type was the most frequently detected, but other types detected included HPV-18, 33, 45 and 52/58. HPV positive tumors tended to be from patients that were >40 and <60 years old (p = 0.07). Thus, the Roche linear array may be used to determine HPV status in FFPE head and neck tumors. Furthermore, our data agree with previous reports indicating that HPV infection is primarily associated with oropharyngeal carcinomas and may not be a risk factor for other HNSCCs. doi:10.1016/j.oos.2009.06.279
O195. The prevalence of human papillomavirus (HPV) in oral and oropharynx squamous cell carcinoma D. Polz *, M. Polz-Dacewicz, K. Morshed Medical University Lublin, Poland The aim of this study was the analysis of the prevalence of HPV infection among patients with oral and oropharynx squamous cell carcinoma (SCC). In the examination group were 60 patients with squamous cell carcinoma, hospitalized in the Department of Otolaryngology Medical University in Lublin. Examination samples were collected from paraffin-block tissues. Patients had not undergone treatment with chemotherapy and radiotherapy. In the examination group there were 21 patients with oral, 24 patients with oropharynx and 24 patients with oral and oropharynx SCC. In statistical analysis Statistic 7.1, test Pearson’s Chi-squared test and calculated odds ratios (OR) were used. In the examination group HPV DNA was found in 25% of patients, in 86.7% HPV type 16. The HPV-infected group was 73% male. The urban inhabitants made up 70%. In the HPV-positive group there were 60% smokers. In all groups there was 58.3% alcohol drinkers. The majority of the tumors were G2 – 81.7%. Statistical significance was found between G2 and SCC among smokers (p = 0.004). In conclusion, the presence of HPV DNA 25% of patients with oral and oropharynx SCC can suggest the role of HPV infection in the pathogenesis of oral and oropharynx SCC. The dominant type at HPV was HPV 16. HPV DNA was detected 5 more often in oropharynx than in oral SCC. Oral and oropharynx squamous cell carcinoma were more often found in patients older than 50 years, smokers and drinkers. doi:10.1016/j.oos.2009.06.280
O196. Human papilloma virus in oral squamous cell carcinomas from young non-smokers in the region of Northern Greece A.K. Kyrgidis a,b,c,*, R.V. Valeri a, G.K. Koloutsos a,b, A.N. Ntomouchtsis a, K.V. Vahtsevanos a, D. Mangoudi b,a a
Department of Oral and Maxillofacial Surgery, Theagenio Cancer Hospital, Thessaloniki, Greece b University Department of Oral Maxillofacial Surgery, Aristotle University of Thessaloniki, Thessaloniki, Greece c Department of Cytology, Theagenio Cancer Hospital, Greece Introduction: Numerous studies have been done to investigate the role of human papilloma viridae (HPV), particularly high-risk genotypes, in anogenital and head and neck cancers. These studies have indicated that the relationship with oral squamous cell carcinoma (OSCC) is less well-defined although some authors concluded
121
that oral infection with HPV, particularly with high-risk genotypes, was considered to be a significant independent risk factor for OSCC. It has been suggested that HPV alter the OSCC distribution and that HPV positive tumors are more frequently reported in younger patients who abstain from smoking and alcohol. Patients and methods: We included only non-smoking, nondrinking young patients in the study. Samples from resected tumor and surrounding tissues were collected in ThiPrep solution (Cytyc UK, Crawley, West Sussex, UK). Fluorescence in situ hybridization (FISH) was subsequently performed to detect HPV DNA for subtypes HPV 16 and 18. Sampling was performed after demographic and clinical selection of patients. Results: We have included so far 12 patients with OSCC, all of whom were younger than 45 years of age and all of whom abstained from smoking and alcohol. Samples were examined for HPV DNA via FISH. We were not able to detect HPV in any of the samples, both from tumors and surrounding tissue. Conclusions: Preliminary findings of the present study are in accordance with findings of gynecological clinics in Northern Greece. It appears that HPV infection may be less frequent in the population of Northern Greece as far as the tested subtypes concerned. Further re-evaluation of our material via polymerase chain reaction and sampling from more individuals are expected to accept or reject the conclusions of this study. doi:10.1016/j.oos.2009.06.281
O197. The aetiopathogenesis of HPV in malignant disease: evidence for a global immunisation programme M.S. Miah *, R.A. Crosbie, R.E. Mountain, S. Mahendran Ninewells Hospital and Medical School, Dundee, United Kingdom Introduction: The evidence collected over the last 25 years suggests that Human Papillomaviruses (HPV) -related malignant disease is rising despite falling incidence in aetiological factors such as smoking. Whilst the role of HPV in cervical carcinomas has been established, there is growing evidence for its role in the aetiopathogenesis of carcinomas involving other systems. In the United Kingdom an immunisation programme against HPV has commenced aimed at adolescent females. Objectives: The aim of this study was to establish the evidence for HPV-related malignant disease in regions other than the cervix and to question whether the evidence would support a global immunisation programme against HPV irrespective of gender or geography. Methods: A literature review of all previous studies and reviews. Searching MEDLINE, PubMed, Embase, and Cochrane Library identified published articles. Two authors to achieve a shortlist of all publications, reviewed abstracts independently. The Australian National Health and Medical Research Council system on levels of evidence in aetiological research was used to categorically analyse information published. Information on incidence of relevant cancers was obtained from the World Health Organisation figures. Results: Between 1982 and November 2008, in excess of 600 studies have been published on the detection and genotyping of HPV in carcinomas outside the cervix. HPV-DNA found in significant proportions of vaginal (90%), vulval (40%), anal (90%) and penile (50%) carcinomas. Of the upper aerodigestive tract carcinomas including the oesophagus, up to 100% are HPV-DNA positive with tonsillar carcinomas demonstrating the strongest association. Up to 95% of HPV-positive carcinomas contain HPV16-DNA. The prevalence of HPV-related malignant disease other than cervical cancer is comparable to that of cervical cancer when disease incidence is taken into consideration.
Oral AbstractsPoster ListOrals ListPan. Disc. & Symp. Abs.Keynote Abs.Keynote Bios.ProgramIAOOWelcomeCommittee Listings
Oral abstracts / Oral Oncology Supplement 3 (2009) 56–122
O195. The prevalence of human papillomavirus (HPV) in oral and oropharynx squamous cell carcinoma D. Polz *, M. Polz-Dacewicz, K. Morshed Medical University Lublin, Poland The aim of this study was the analysis of the prevalence of HPV infection among patients with oral and oropharynx squamous cell carcinoma (SCC). In the examination group were 60 patients with squamous cell carcinoma, hospitalized in the Department of Otolaryngology Medical University in Lublin. Examination samples were collected from paraffin-block tissues. Patients had not undergone treatment with chemotherapy and radiotherapy. In the examination group there were 21 patients with oral, 24 patients with oropharynx and 24 patients with oral and oropharynx SCC. In statistical analysis Statistic 7.1, test Pearson’s Chi-squared test and calculated odds ratios (OR) were used. In the examination group HPV DNA was found in 25% of patients, in 86.7% HPV type 16. The HPV-infected group was 73% male. The urban inhabitants made up 70%. In the HPV-positive group there were 60% smokers. In all groups there was 58.3% alcohol drinkers. The majority of the tumors were G2 – 81.7%. Statistical significance was found between G2 and SCC among smokers (p = 0.004). In conclusion, the presence of HPV DNA 25% of patients with oral and oropharynx SCC can suggest the role of HPV infection in the pathogenesis of oral and oropharynx SCC. The dominant type at HPV was HPV 16. HPV DNA was detected 5 more often in oropharynx than in oral SCC. Oral and oropharynx squamous cell carcinoma were more often found in patients older than 50 years, smokers and drinkers. doi:10.1016/j.oos.2009.06.280
O196. Human papilloma virus in oral squamous cell carcinomas from young non-smokers in the region of Northern Greece A.K. Kyrgidis a,b,c,*, R.V. Valeri a, G.K. Koloutsos a,b, A.N. Ntomouchtsis a, K.V. Vahtsevanos a, D. Mangoudi b,a a
Department of Oral and Maxillofacial Surgery, Theagenio Cancer Hospital, Thessaloniki, Greece b University Department of Oral Maxillofacial Surgery, Aristotle University of Thessaloniki, Thessaloniki, Greece c Department of Cytology, Theagenio Cancer Hospital, Greece Introduction: Numerous studies have been done to investigate the role of human papilloma viridae (HPV), particularly high-risk genotypes, in anogenital and head and neck cancers. These studies have indicated that the relationship with oral squamous cell carcinoma (OSCC) is less well-defined although some authors concluded
121
that oral infection with HPV, particularly with high-risk genotypes, was considered to be a significant independent risk factor for OSCC. It has been suggested that HPV alter the OSCC distribution and that HPV positive tumors are more frequently reported in younger patients who abstain from smoking and alcohol. Patients and methods: We included only non-smoking, nondrinking young patients in the study. Samples from resected tumor and surrounding tissues were collected in ThiPrep solution (Cytyc UK, Crawley, West Sussex, UK). Fluorescence in situ hybridization (FISH) was subsequently performed to detect HPV DNA for subtypes HPV 16 and 18. Sampling was performed after demographic and clinical selection of patients. Results: We have included so far 12 patients with OSCC, all of whom were younger than 45 years of age and all of whom abstained from smoking and alcohol. Samples were examined for HPV DNA via FISH. We were not able to detect HPV in any of the samples, both from tumors and surrounding tissue. Conclusions: Preliminary findings of the present study are in accordance with findings of gynecological clinics in Northern Greece. It appears that HPV infection may be less frequent in the population of Northern Greece as far as the tested subtypes concerned. Further re-evaluation of our material via polymerase chain reaction and sampling from more individuals are expected to accept or reject the conclusions of this study. doi:10.1016/j.oos.2009.06.281
O197. The aetiopathogenesis of HPV in malignant disease: evidence for a global immunisation programme M.S. Miah *, R.A. Crosbie, R.E. Mountain, S. Mahendran Ninewells Hospital and Medical School, Dundee, United Kingdom Introduction: The evidence collected over the last 25 years suggests that Human Papillomaviruses (HPV) -related malignant disease is rising despite falling incidence in aetiological factors such as smoking. Whilst the role of HPV in cervical carcinomas has been established, there is growing evidence for its role in the aetiopathogenesis of carcinomas involving other systems. In the United Kingdom an immunisation programme against HPV has commenced aimed at adolescent females. Objectives: The aim of this study was to establish the evidence for HPV-related malignant disease in regions other than the cervix and to question whether the evidence would support a global immunisation programme against HPV irrespective of gender or geography. Methods: A literature review of all previous studies and reviews. Searching MEDLINE, PubMed, Embase, and Cochrane Library identified published articles. Two authors to achieve a shortlist of all publications, reviewed abstracts independently. The Australian National Health and Medical Research Council system on levels of evidence in aetiological research was used to categorically analyse information published. Information on incidence of relevant cancers was obtained from the World Health Organisation figures. Results: Between 1982 and November 2008, in excess of 600 studies have been published on the detection and genotyping of HPV in carcinomas outside the cervix. HPV-DNA found in significant proportions of vaginal (90%), vulval (40%), anal (90%) and penile (50%) carcinomas. Of the upper aerodigestive tract carcinomas including the oesophagus, up to 100% are HPV-DNA positive with tonsillar carcinomas demonstrating the strongest association. Up to 95% of HPV-positive carcinomas contain HPV16-DNA. The prevalence of HPV-related malignant disease other than cervical cancer is comparable to that of cervical cancer when disease incidence is taken into consideration.
Oral AbstractsPoster ListOrals ListPan. Disc. & Symp. Abs.Keynote Abs.Keynote Bios.ProgramIAOOWelcomeCommittee Listings
Oral abstracts / Oral Oncology Supplement 3 (2009) 56–122