Occurrence and risk factors for CABG interventions after PTCA revascularization

ABSTRACTS / Journal of Molecular and Cellular Cardiology 42 (2007) S238–S246

has a local, tissue-specific role and functions in vascular calcium sensing. Keywords: Calcium handling proteins; Imaging doi:10.1016/j.yjmcc.2007.03.728

Glutathione reductase and vitamin B2 status in red cells of pregnant women A. Kozlovskayaa, N. Potolitsynab, E. Bojkob. aCardiological Centre of Komi Republic, Syktyvkar, Russia. bInstitute of Physiology, Komi Science Center, Ural division Russian Academy of Sciences, Russia Pregnancy complications may correlate to activation of reactive oxygen species (ROS) generation partially in lacking of antioxidant enzymes (AE) activity. Glutathione reductase (GSH-RX, k.f.1.6.4.2) plays an important role in the antioxidant protection. It contains Vitamin B2 (Riboflavin) as its coenzyme. The GSH-RX activity in mothers' red cells during a normal pregnancy remains not clear. Previous studies showed different results. Materials and methods: Glutathione reductase activity and vitamin B2 providing (FAD-effect) were analyzed (Vuilleumier et al., 1990) in red cells of healthy pregnant woman according pregnancies terms. Differences were tested using Wilcoxon test. Results: The level of GSH-RX activity in 17–22 weeks of pregnancy was strictly lowered and became the highest in 38– 40 weeks. The highest value of Fad-effect was found out in 27– 37 weeks of pregnancy. Conclusion: Our dates showed that GSH-RX activity in mothers' red cells lowered in second trimester of pregnancy. There was no correlation between FAD-effect and GSH-RX activity (FAD-contain enzyme) in pregnant woman. Detected peculiarities of GSH-RX activity during pregnancy may show possible influence of placenta–fetus complex. Keywords: Antioxidants; Protection doi:10.1016/j.yjmcc.2007.03.729

Application of anti-human cTnI monoclonal antibodies in evaluation of rat myocardial injury models ZhiPing Bian, Di Yang, XiangJian Chen, JinDan Xu, JiNan Zhang. First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, China To determine the diagnostic value of cTnI for detecting rat myocardial injury, 11 anti-human cTnI monoclonal antibodies identified for rat cTnI were selected by Western Blot. Six antibodies recognized rat cTnI, two of them, JS14 and JS15 were chosen to be the partners for ELISA assay, and applied for immunohistochemical staining respectively for localization of

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cTnI distribution in rat heart with or without myocardial injury. SD rats (n = 10) were subcutaneously injected with isopenaline (ISO) at a single dose of 8 mg/kg. Another 10 SD rats received saline as control. Four hours after the injection, the rats blood was collected. The established ELISA assay was carried out for the detection of serum cTnI from the rats. The level of serum cTnI from rats treated with ISO was statistically higher than that of control rats (1.28 ± 0.78 vs 0.30 ± 0.22, p < 0.01). The pathological examination showed myofibril rupture and infiltration with neutrophil granulocytes and interstitial oedema in left ventricle from rats treated with ISO. The hearts of the control rats showed column shape, stained nuclear indigo and plasma rosiness. Immunohistochemical study showed that the myocardium of control rats was stained brown evenly, while that from the treated rats presented incomplete staining. In conclusion, cTnI can be used to evaluate myocardial injury from rats. Keywords: Cardiac troponin I; Myocardial injury; Immunohistochemistry doi:10.1016/j.yjmcc.2007.03.730

Occurrence and risk factors for CABG interventions after PTCA revascularization I. Craciunescu, G. Pampu, M. Serban, S. Vasile, B.A. Popescu, H. Moldovan, D. Deleanu, C. Ginghina. Institute of Cardiovascular Diseases, Bucharest, Romania Background: Coronary reinterventions for myocardial revascularization have become common and we frequently meet an increasing number of mixed revascularization procedures. Objective: To better understand the decisions of changing the first method of revascularization, we determined the occurrence and risk factors of coronary artery by-pass grafts (CABG) interventions after percutaneous transluminal coronary angioplasty (PTCA). Methods: We studied patients referred to PTCA in our clinic over a period of 2 years. Repeated revascularizations (PTCA or CABG) were registered in a 2 years follow-up period. We analyzed traditional risk factor, clinical evolution, coronaries particularities. Results: From 1892 patients who benefit from PTCA, 363 patients (19.1%) had repeated revascularization (group A-333 pts. with repeated PTCA and group B-30 pts. with CABG). There were no difference between the two groups regarding the prevalence of risk factors. Evolution after the primary PTCA was more severe in group B (acute coronary syndromes: 46.6% in group B and 30.9% in group A, p < 0.01, myocardial infarction: 10% in group B, 3.0% in group A, p < 0.001). Patients from group B were more likely to have significant lesions on left descendent artery (LAD) (93.3% versus 68.1%, p < 0.001), proximal end-of-stent restenosis (23.3% versus 9.3%, p < 0.01), trivascular lesions (20% versus 14.1%, p=0.028) and restenosis involving an important branch (13.3% versus 6.9%, p < 0.001). Conclusion: Patients with CABG following PTCA have a more severe clinical evolution and different coronarographic

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ABSTRACTS / Journal of Molecular and Cellular Cardiology 42 (2007) S238–S246

characteristics that determine the selection of revascularization method. Keywords: Ventricular function; Ischemia doi:10.1016/j.yjmcc.2007.03.731

TIMI risk score in patients with unstable angina/non-st elevation mi who underwent CABG revascularization G. Pampu, I. Craciunescu, M. Serban, S. Vasile, H. Moldovan, D. Deleanu, C. Ginghina. Institute of Cardiovascular Diseases, Bucharest, Romania Background: TIMI risk score has not been yet tested in special categories as patients revascularized by CABG. Objective: The purpose of the study was to investigate the relationships between the TIMI risk score and clinical outcome in patients with UA/NSTEMI and CABG revascularization. Methods: 158 patients with UA/NSTEMI who underwent myocardial revascularization by CABG were enrolled and followed-up over a 2 years period. We registered all-cause mortality, the major cardiac adverse events (MACE), the patency of coronary grafts and the necessity of repeated revascularization. Results: Patients were divided into 4 groups, according to the number of MACE and consecutive hospital admissions after the CABG. Mean values of TIMI risk score were 3.16 in group a with 0/1 MACE, 4.75 in group B with 2/3 MACE, 5.23 in group C with 4/5 MACE and 6.27 in group D with ≥ 6 MACE (P < 0.001 by ANOVA analyse). All patients underwent coronarographic control after CABG. Highest TIMI risk score were registered in patients with all grafts occluded (mean value of 6.33 ± 1.26). Cut off value of TIMI risk score for patency of venous grafts was 3.35, with a specificity of 86.6% and a sensitivity of 82.1%. Patients with repeated revascularization within 2 years after the first CABG (either repeated CABG or PTCA) had a higher TIMI risk score (7.12 ± 2.87 versus 3.06 ± 1.23, p < 0.001). Conclusion: TIMI risk score is a valuable predictor of clinical outcome and patency of grafts in patients with UA/NSTEMI who underwent CABG revascularization. Keywords: Ventricular function; Ischemia doi:10.1016/j.yjmcc.2007.03.732

Interrelationships of functional, biochemical and morphological variables in catecholamine cardiotoxicity Přemysl Mladěnka, Radek Hrdina, Zuzka Bobrovová, Mojmír Hübl, Petr Nachtigal, Jan Škrle, Vladimir Semecký. Charles Univ., Fac Pharmacy, Czech Republic Coronary heart disease and mainly its most serious form– acute myocardial infarction (AIM)–represent in spite of large

cardiovascular research still the most common cause of death in the developed countries. Isoprenaline (ISO), a synthetic catecholamine, has the ability to evoke myocardial impairment in some aspects very similar to AIM in human. This study was designated to verify these injuries from biochemical, functional and histological point of views and to establish correlations among different parameters. 18 male Wistar rats were divided into two groups—control and ISO. After 24 h of ISO (100 mg/ kg s.c.) or saline administration, measurements were performed. Very significant differences were shown in various parameters between both groups. Shortly, ISO brought about significant impairment of myocardial function, marked elevation of cardiac troponin T in plasma, calcium overload in the myocardium and was associated with myocardial necrosis. Some significant correlations were revealed, in particular, of cardiac troponin T with calcium overload and functional variables. In conclusion. ISO-myocardial injury may be a suitable model of AIM, in addition, cardiac troponin T could precisely indicate the extent of functional damages of the myocardium. Acknowledgments This work was supported by grants 98/2005/C/FaF and 94/ 2006/C/FaF of Charles University in Prague (Czech Republic). Keywords: Infarction; Cardiomyopathies; Calcium doi:10.1016/j.yjmcc.2007.03.733

Myocardial ischemia prevention in swine with autologous ozoneized blood transfusion Andrea Barone, Javier Woskoboinic, Aníbal Grangeat, Ricardo Pérez, Ignacio Berra, Guillermo Berra, José Milei. ININCA, Univ Buenos Aires, Argentina Although ozone therapy has been widely used, no attempts have been made to evaluate its protective effects in acute myocardial ischemia. Because of this, a pilot study with a few number of animals was initiated. Under surgical conditions, 200 ml of autologous venous blood, enriched with 20 ml ozone (major autohemotransfusion—MAHT) were infused through the internal jugular vein of adult swines (n = 3), 10 min before the proximal right coronary artery was occluded with a balloon for angioplasty. All animals were sacrificed immediately after having completed the sequence: ST segment elevation, ventricular fibrillation and cardiac arrest. No evident necrotic/ ischemic areas as assessed by tripheniltetrazolium chloride (TTC) were detected and histological sections revealed no damage in myocardial tissue. Conversely, swine infused with plain autologous venous blood (n = 3) and submitted to the same protocol showed preservation in only 81.4 ± 11.6% of the total myocardium, with infarcted/ischemic areas of 14 ± 8.5% and 4.5 ± 3.1%, respectively, (TTC stain), while diffuse monofocal–multifocal necrotic areas were found in microscopical