Oral abstracts contents list

Journal of Clinical Virology 28S (2003) S1–S6

Oral abstracts contents list Oral Presentation 1 (OP1)

OP1-4

Monday 8 December 2003

Protein expression of dengue virus type 2 in twodimensional gel electrophoresis

OP1-1 Integrated epidemiology of DHF in Thailand P. Barbazan1 , M. Souris1 , N. Nitatpattana2 , S. Yoksan2 , J.P. Gonzalez1 1 UR034 IRD Emerging Viral Diseases and Information Sys-

tem Research, Mahidol University, Thailand; 2 Center for Vaccine Development, Mahidol University, Nakhon Pathom Province 73170, Thailand OP1-2 Traveling waves in dengue hemorrhagic fever incidence in Thailand Derek Cummings1 , Rafael Irizarry2 , Norden Huang3 , Timothy Endy4 , Ananda Nisalak5 , Kumnuan Ungehusak6 , Donald Burke1 1 Department

of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, USA; 2 Department of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, USA; 3 Laboratory for Hydrospheric Processes, NASA Goddard Space Flight Center, Greenbelt, USA; 4 Virology Division, United States Army Medical Research Institute in Infectious Disease, Fort Detrick, USA; 5 Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; 6 Department of Disease Control, Ministry of Public Health, Thailand OP1-3 Identification of neutralizing monoclonal antibodies to dengue viruses R.R. Matias1,2 , D.J.M. Cruz1 , E.P. Raymundo2 , F.F. Natividad1 1 Research

and Biotechnology Division, St. Luke’s Medical Center, Quezon City, Philippines; 2 University of the Philippines-Diliman, Quezon City, Philippines2

1386-6532/$ – see front matter doi:10.1016/j.jcv.2003.09.005

S.Y. Muliawan1,2 , S.K. Lam1 , O.H. Hashim2 , R. Yusof2 1 Department

of Medical Microbiology, Kuala Lumpur, Malaysia; 2 Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Malaya, Kuala Lumpur, Malaysia OP1-5 Follow-up of Thai school children immunized with live attenuated tetravalent dengue Thai-Mahidol strains 3–8 years ago: current immunity response and occurrence of DF/DHF P. Chanthavanich1 , G. Luxemburger2 , S. Yoksan3 , J. Lang2 1 Faculty

of Tropical Medicine, Mahidol University, Bangkok, Thailand; 2 Aventis Pasteur, Lyon, France; 3 Center for Vaccine Development, Mahidol University, Nakhonpathom, Thailand OP1-6 Long-term surveillance of subjects immunized with live attenuated tetravalent dengue vaccine in Thailand Arunee Sabchareon1 , Jean Lang2 , Sutee Yoksan3 , Pornthep Chanthavanich1 , Remi Forrat2 , Phanorsri Attanath1 , Natth Shamampravati3 1 Vaccine

Trial Canter, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; 2 Aventis Pasteur, Lyon, France; 3 Center for Vaccine Development, Institute of Sciences and Technology for Research and Development, Nakhonpathom, Thailand OP1-7 Identification of potential peptide vaccine candidates against dengue virus by phage display M.I.M.Caperig, C.P. Dapat, R.R. Matias, F.F. Natividad Research and Biotechnology Division, St. Luke’s Medical Center, Quezon City, Philippines

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Oral abstracts / Journal of Clinical Virology 28S (2003) S1–S6

OP1-8

OP2-5

Development of a tetravalent dengue DNA vaccine

Serological and molecular diagnosis of Crimean–Congo haemorrhagic fever disease in Iranian suspected patients

S.C. Liew1 , M.P. Reid2 , J.G. Aaskov1,2 1 Center

for Molecular Biotechnology, Queensland University of Technology, Brisbane, Australia; 2 Australian Army Malarial Institute, Brisbane, Australia

S. Chiniker1 , A. Fayaz1 , R. Mirahmadi1 , V. Mazaheri1 , F. Ahmadnejad, MS1 , B. Hooshmand2 , P. Nabeth1 , M.F. Saron3 1 Pasteur

Institute of Iran, Iran; 2 Center of Disease Control of Iran, Iran; 3 Pasteur Institute of Dakar, Dakar, Iran

Oral Presentation 2 (OP2) OP2-1

OP2-6

Spread of Murray valley encephalitis virus within Australia D.W. Smith1,2 1 The

Western Australian Center for Pathology and Medical Research, Nedlands, Australia; 2 The University of Western Australia, Nedlands, Australia OP2-2

Nathalie Charlier 1 , Richard Molenkamp2 , Pieter Leyssen1 , Jan Paesbuyse1 , Christian Drosten3 , Erik De Clereq1 , Peter J. Bredenbeek2 , Johan Neyts1 1 Laboratory

Differential expression profiles of west Nile virus-infected host cells Wee Lee Koh, Mah Lee Ng Flavivirology Laboratory, Department of Microbiology, 5 Science Drive 2, National University of Singapore, Singapore 117597, Singapore OP2-3 Serological surveillance of Japanese encephalitis virus infection in the Philippines Alonzo1 ,

The PRM and E envelope glycoproteins of the modoc virus, a murine flavivirus belonging to the “not known vector” cluster, render yellow fever virus (17D) neuroinvasive for mice

Inoue1,2 ,

Cruz1 ,

M.T. S. D.J.M. Matias1,3 , K. Morita2 , F.F. Nativida1

F.

Tadena1 ,

R.R.

1 Research

and Biotechnology Division, St. Luke’s Medical Center, Quezon City, Philippines; 2 Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan; 3 Institute of Biology, University of the Philippines-Diliman, Quezon City, Philippines OP2-4 Functional and structural analysis of neutralizing epitopes on the Japanese encephalitis virus envelope protein Cheng-WenLin1,2 , Suh-Chin Wu2 1 Department of Medical Laboratory Science and Biothechnology, China Medical University, Taichung, Taiwan; 2 Institute of Biotechnology and Department of Life Science, National Tsing-Hua University, Hsinchu, Taiwan

of Virology and Chemotherapy, Rega Institute for Medical Research, Leuven, Belgium; 2 Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands; 3 Bernhard Nocht Institute of Tropical Medicine, Hamburg, Germany OP2-7 Molecular epidemiology of hantavirus in China Shiwen Wang, Changshou Hang, Yanxiang Xie, Hua Wang, Benjiang Ma Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, China OP2-8 Emerging and re-emerging viruses in northern Eurasia and their intercontinental connection D.K. Lvov, D.I. Ivanovsky Institute of Virology, Moscow, Russia Oral Presentation 3 (OP3) OP3-1 Resistance mutations following a single-dose intrapartum administration of nevirapine to HIV-infected Thai women and their infants receiving short-course zidovudine W. Leelawiwat1 , T. Chotpitayasunondh2 , N. Vanprapar3 , T. Tasaneeyapan1 , M. Culnane4 , A. Teeraratkul1 , K. Necyapun1 , B. Jetsawang1 , J. Tappero4 , T. Chaowanachan1 1 The

Thailand MOPH-US CDC Collaboration, Nonthabud, Thailand; 2 Queen Sirikit National Institute of Child Health,

Oral abstracts / Journal of Clinical Virology 28S (2003) S1–S6

S3

Bangkok, Thailand; 3 Siriraj Hospital, Mahidol University, Bangkok, Thailand; 4 Centers for Disease Control and Prevention, Atlanta, USA

OP3-7

OP3-2

Y. Huang, J. Larkin, L. Jin, M. Farmen, D. Venable, J.I. Glass, S.L. Tan

Genetic and demographic characterization of Indonesian HIV cases from 1993 to 2000 Listiyaningsih1 ,

McArdle1 ,

Graham1 ,

Erlin James Robert Mondastri Sudarijo4 , Kevin Porter3 , Wuryadi Suharyono1 , Andrew Corwin1 , Surijadi Gunawan2 , Charmagne Beckett1 (US Naval Medical Research Unit No. 2, Jakarta, Indonesia) 1 National

Institute of Health Research and Development, MOH, Jakarta, Indonesia; 2 Naval Medical Research Center, Silver Spring, MD, USA; 3 Faculty of Public Health, University of Indonesia, Depok, Indonesia OP3-3 A novel EGFP-CEM-NK flow cytometric method for measuring ADCC activity in HIV-infected individuals

How hepatitis C virus outmaneuvers the host interferon defense: implications for hepatitis C therapeutics

Inflammation and Immunomodulation Research, Eli Lilly and Company, Indianapolis, USA OP3-8 Genotyping HCV strains from India by RFLP and phylogenetic analysis of 5
non-coding region Ajoy V. Bhupatiraju1 , Dhrubaa Ghosh1 , Shobha Broor1 , S.L. Broor2 , Ranjana Anand3 , Aravind Rai3 1 All

India Institute of Medical Sciences, New Delhi, India;

2 Indraprastha Apollo Hospitals, New Delhi, India; 3 National

Institute of Communicable Diseases, Delhi, India Oral Presentation 4 (OP4)

Wannee Kantakamalakul1 , Surat Jongrakthaitae1 , Silawun Ampol1 , Kovit Pattanapanyasat2

OP4-1

1 Department of Microbiology, Mahidol University, Bangkok,

Molecular epidemiology of imported measles cases from Vietnam in Korea, 2003

Thailand; 2 Office for Research and Development, Center of Excellence for Flow Cytometry, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand OP3-4 Evaluating HIV-1 drug resistance mutations prior to the introduction of new antiretrovirals into the clinic environment Choo Beng Chew, Bin Wang, Chanh Op Shaw, Nitin Saksena, Dominic E. Dwyer CIDMLS, ICPMR, Westmead Hospital, Center for Virus Research, Westmead Millennium Institute, Westmead, NSW, Australia OP3-5 20 Years hepatitis a sero-epidemiology in Belgium R. Vranckx

Y.S. Chung, M.K. Kim, K.A. Kim, Y. Kim, J.S. Koo, J.Y. Lee, M.S. Park, H.W. Cho, C. Kang Department of Virology, National Institute of Health, Korea OP4-2 Multisystemic complication of measles: measles gastritis and duodenitis, appendicitis and peritonitis, cholecystitis and gall bladder lthiasis Jair Vargas, Alfonso J. Rodr´ıguez, Rosa Barbella Collaborative Group of Clinical and Epidemiological Virology Research (CGCEVR), Caracas, Venezuela OP4-3 Rapid detection and simultaneous genotyping of influenza a virus by real time PCR

Institute of Public Health, Brussels, Belgium

Kok Tuck Weng1 , Belinda Stone1 , Julie Burrows1 , Sonia Schepetiuk1 , Alan Hampson2 , Robert Shaw2 , Geoff Higgins1

OP3-6

1 Institute

Automated real-time RT-PCR assay for detection and quantitation of hepatitis C virus RNA in clinical samples using magna pure LC and lightcycler system Byoung-Guk Kim, Hye-Sung Jeong, Sun-Young Baek, Jin-Ho Shin, Sue-Nie Park Korea Food and Drug Administration, Seoul, Korea

of Medical and Veterinary Science, Adelaide, South Australia, Australia; 2 WHO Collaborating Center for Reference and Research on Influenza, Melbourne, Australia

S4

Oral abstracts / Journal of Clinical Virology 28S (2003) S1–S6

OP4-4

Oral Presentation 5 (OP5)

Genetic characterization of oseltamivir resistance of influenza viruses (A/H1N1) isolated in Korea during 2001–2002 season

OP5-1

Y.Y. Kim1,2 , J.Y. Lee1 , J.H. Hwang1 , K.A. Kim1 , S.W. Jang1 , Y.S. Chung1 , H.H. Lee2 , M.S. Park1 , C. Kang1 1 Laboratory of Respiratory Viruses Department of Virology,

National Institute of Health, Seoul, Korea; 2 Division of Biological Sciences, Konkuk University, Seoul, Korea OP4-5 Standardization of RT-PCR of a newer causative agent of ARI: human metapneumoviruses in India D. Ghosh1 , W. Sullender2 , S. Broor1 1 Department

of Microbiology, All India Institute of Medical Sciences, New Delhi, India; 2 Department of Microbiology and Pediatrics, UAB School of Medicine, UA-B, Birmingham, USA OP4-6 Entry and replication processes of SARS coronavirus Mah-Lee Ng1,3 , E’ein See2 , Eng-Eong Ooi2 , Suat-Hoon Tan3 , Ai-Ee Ling4 1 Department of Microbiology, National University of Singa-

pore, Singapore; 2 Environmental Health Institute, National Environment Agency, Singapore; 3 Electron Microscopy Unit, Faculty of Medicine, National University of Singapore, Singapore; 4 Department of Pathology, Singapore General Hospital, Singapore OP4-7 The use of an in-house RT-PCR assay for type-specific dengue virus detection in the differential diagnosis of patients with possible SARS Chiu Lily1 , Leow Gek Har1 , Chow T.K. Vincent2 , Paul A. Tambyah3 , Koay S.C. Evelyn1,4 1 Molecular Diagnosis Center, Department of Laboratory Medicine, National University Hospital, Singapore; 2 Department of Microbiology, National University of Singapore, Singapore; 3 Department of Medicine, National University of Singapore, Singapore; 4 Department of Pathology, National University of Singapore, Singapore

OP4-8 Lactobacillus as oral vaccine carrier against coronavirus Phui-San Ho1 , Yuan-Kun Lee1 , Jimmy Kwang2 1 Department

of Microbiology, National University of Singapore, Singapore; 2 Temasek Life Sciences Laboratory, Singapore

Mapping of domains responsible for nucleocapsid protein–phosphoprotein interaction of henipaviruses Y.P. Chan1 , L.F. Wang2 , C.L. Koh1 , S.K. Lam3 1 Institute

of Biological Sciences (Genetics), Universiti Malaya, 50603 Kuala Lumpur, Malaysia; 2 CSIRO, Livestock Industries, AAHL, Geelong, Australia; 3 Department of Medical Microbiology, Universiti Malaya, 50603 Kuala Lumpur, Malaysia OP5-2 A golden hamster model for human acute NIPAH virus infection K.T. Wong1 , I. Grosjean2 , C. Brisson3 , B. Blanquier4 , M. Fevre-Montange3 , A. Bernard3 , P. Loth2 , M.C. Georges-Courbot2 , M. Chevailier5 , H. Akaoka3 , P. Marianneau2 , S.K. Lam1 , T.F. Wild4 , V. Deubel2 1 Faculty

of Medicine, University of Malaya, Malaysia; Pasteur, Center de Recherche Mérieux-Pasteur, France; 3 INSERM U.433, France; 4 INSERM U.404, CERVI, France; 5 Lab M Mérieux, Lyon, France

2 Institute

OP5-3 Minimum protein requirements for transcription and RNA replication of a minigenome of NIPAH virus K. Halpin1,2 , B. Bankamp1 , P.A. Rota1 , B.H. Harcourt1 , W.J. Bellini1 1 Division

of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, USA; 2 Australian Animal Health Laboratory, Geelong, Australia2 OP5-4 Not all fusogenic bat viruses are henipaviruses: an untrastructural summary of bat viruses isolated from Australian and Malaysian bats A.D. Hyatt1 , T. Wise1 , K.B. Chua2 , I. Pritchard1 , R. Weir3 , L.Wang1 1 Australian

Animal Health Laboratory, Geelong, Australia; of Malaya Medical Center, Kuala Lumpur, Malaysia; 3 Berrimah Veterinary Laboratories, Berrimah, Australia

2 University

Oral abstracts / Journal of Clinical Virology 28S (2003) S1–S6

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OP5-5

OP6-2

Features of viral RNA synthesis in cells infected with HENDRA virus: an investigation by quantitative real-time PCR

Significance of herpes simplex virus (HSV) and human cytomegalovirus (CMV) in Pemphigus vulgaris cases with oral lesions

Peter J. Wright1,2 , Gary Crameri1 , Bryan T. Eaton1

R.K. Ratho2 , A. Kaira1 , K. Inderjit1 , Bhushan Kumar1

1 CSIRO

Livestock Industries, Australian Animal Health Laboratory, Geelong, Australia; Monash University, Clayton, Australia2

1 Departments

of Virology and Dermatology, Venereology and Leprology, India; 2 Postgraduate Institute of Medical Education and Research, Chandigarh, India

OP5-6

OP6-3

Tioman virus, a novel paramyxovirus, is capable of inducing apoptosis in vitro and in vivo

Brainstem encephalitis due to primary herpes simplex virus 2 infection in a young woman

K.G. Yaiw1 , K.T. Wong1 , D. Shamala1 , K.B. Chua2 , L.F. Wang3

J.W. Tang1 , L.J. Ward1 , N.W.S. Davies2,3 , A.M. Geretti4 , R.S. Howard2 , N.P. Hisch2 , K.N. Ward1

1 Department

1 Royal

of Pathology and Department of Medical Microbiology, University of Malaya, Malaysia; 2 Ministry of Health, Malaysia; 3 CSIRO, Geelong, Australia OP5-7

Free and University College Medical School, London, UK; 2 National Hospital for Neurology and Neurosurgery, London, UK; 3 Guy’s, King’s, St. Thomas’ School of Medicine, London, UK; 4 King’s College Hospital (Dulwich), London, UK

Genetic analysis of a human group B rotavirus detected recently in China

OP6-4

N. Kobayashi1 , A. Sumi1 , M. Ishino1 , K. Mise1 , K. Kojima1 , J.H. Yang2 , Y.H. Wang2 , D.J. Zhou2

Epstein Barr virus latent membrane protein 1: genotypic and antigenic differences in relation to nasopharyngeal carcinoma

1 Sapporo

Medical University School of Medicine, Sapporo, Japan; 2 Wuhan Centers for Disease Prevention and Control, Wuhan, China

E.L. Tan, C.K. Sam NPC Laboratory, University of Malaya, Kuala Lumpur, Malaysia

OP5-8 Diagnosis of enteroviral meningitis by reverse transcriptase polymerase chain reaction (RT-PCR) and genotyping of clinical isolates of enteroviruses from Kuwait A. Dalwai, S. Ahmad, S. George, A. Pacsa, W. AI-Nakib Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait Oral Presentation 6 (OP6) OP6-1 Herpes simplex type 1 shedding is associated with reduced hospital survival in patients receiving assisted ventilation in a Tertiary Referral Intensive Care Unit G.M. Ong1 , K. Lowry2 , S. Mahajan2 , D.E. Wyatt1 , C. Simpson3 , H.J. O’Neill1 , C. McCaughey1 , P.V. Coyle1 1 Regional

Virus Laboratory, UK; 2 Regional Intensive Care Unit, UK; 3 Department of Microbiology—Queens University Belfast, Royal Group of Hospitals Trust, Grosvenor Road, Belfast BT12 6BA, UK

OP6-5 An alteration of neurotransmitter transporter gene expression in experimental rabies-infected mice Jitra Kasisith1 , D.M.V. Veera Tepsumethanon2 , Sukathida Ubol1 1 Department

of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; 2 Queen Soavabha Memorial Institute, Bangkok, Thailand OP6-6 A reverse genetics system for Australian bat lyssavirus David Warrilow, Ina L. Smith, Greg A. Smith Public Health Virology Laboratory, Queensland Health Scientific Services, Brisbane, Australia

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Oral abstracts / Journal of Clinical Virology 28S (2003) S1–S6

OP6-7

OP6-8

Recovery from poxvirus infections: role of antibody and B cells

The recombinogenic activity of feline leukemia virus subgroup A is dependent on endogenous FELV-like RNA expression

Vijay Panchanathan, Geeta Chaudhri, Gunasegaran Karupiah Infection and Immunity Group, Division of Immunology and Genetics, John Curtin School of Medical Research, Australian National University, Canberra, ACT 2601, Australia

Musaad A. AL-dubaib1 , Kathleen A. Hayes2,3 , Andrew J. Phipps2,3 , Pradip Roy-Burman5 , Lawrence E. Mathes2,3,41 King saud University, Buriedah, Saudi Arabia; 2 Department of Veterinary Biosciences, USA; 3 The Center for Retrovirus Research, USA; 4 Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA; 5 Department of Pathology, School of Medicine, University of Southern California, Los Angeles, CA, USA