Peptone shock in rabbits

Peptone

Shock in Rabbits.

(btzl,

1,‘. W., :tu~l Dragst edt.

(I. A.:

.I. Pharmacol.

&

Tiecause of the similarity I)etweon petrtone shock and :rn:rtrh~-laxis in rahhita, the changes which occur in tlrc c~oncentrntion of histamine in the hlootl and plasma dnaphyl;wtic~ shock in rabbits is characterized hy :I in peptonc shock were stutlietl. marked leucopmia and a dwreasc~ in total hlootl histamine. The addition of antigen to the hlootl of sensitized rahhits, in vitro, pro~luces a wleasr of histamine from the I)lOOtl cells t0 the I)lilS?ll8,. The intravcnour injwtion in hlood lristaminc :1ntl a heparinizrd hlootl of normal cells to tllr platsr11:c. ‘~‘hrrrforr, histamine and upon tltc mhitc following xnaphyluxis in tlw the injection of peptone into anaphylaxis.

Experimental Am.

.I. Path.

In an substances, commonly this type,

of pro1 cases in rahhits pro~luw~l :I tnarlied r,eduction motleratc Ieucopenia. The atltlition of pcptone to rabbits cauwl :I rtlr:n+ of hixtaminc from the blood the etfects of pcptonc sltock upon the total blootl hlootl cell count in rahhits arc similar to ilrosc ohservrrl same animal. H~ww~r, the gcnwrl symptoms following rabbits were not so severe ah those owuwing (luring

Focal Necrosis of the Liver. 18:

323,

lI:Ldley,

Ct., ,Tr., and Lushhaugh,

B. S.:

7942.

effort to ~lrtcrminc whether throtnhosis or both, wrr responsihlc for the f(JGll observed in i nf’wtious and toxic tlirwses, experimentally. in hypersensitive animals.

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Rabbits and guinea pigs were sensitizwl to crystalline egg alhurnin. Thr antigen was then injected into the mrscnteric veins, tltc m:rrgin:rl ear veins, the abdominal cavity, and the spleen. Thohe animals which survived the an:tphylactic* sltotk wre sacrificed, at rarvinI b7 times , following the injection of the antigen. Hpecimens of liver tissue were taken at the time of the first operation and after the anim:lls were killed. Rabbits sensitized witlt crystalline egg albumin showed large arcas of coagulatetl necrosis of t,lie liver parenchynm, following the injection of the antigen into the peritoneal cavity, into the spleen, or into the mesmteric or marginal ear veins. Control groups treated with nonrelated antigen failed to slrow these lesions. Sensitized guinea pigs, which received injections of crystalline egg albumin into the mrsentoric veins, showed similar hepatic lesions. Vascular thrombosis was rarely present and apparently was second:trg to necrosis of the hepatic cells. The portal veins and the sinusoids ~howrtl ~~olyn~or~~l~onuclear congestion prior to tlw onset of In the interlobular lesions Ihere was no evidence of hemornecrosis of the liver. rhage, edema, or change in the vascular endotlreliurn. It was concluded that focal necrosis of the liver was a direct result of the loc:~l union of antigen and antibody. The coagulative necrosis, the leucocytic response, ant1 the infrequent tluonthosis of the adjacent blood vessels were regarded as secondary to the antigen-antibody reaction.