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β-catenin signaling pathway
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Abstracts / Journal of the Neurological Sciences 333 (2013) e215–e278
Conclusion: Phosphorylation of Caveolin-1 could attenuate OGDinduced apoptotic cell death in brain microvascular endothelial cells and its mechanisms could be associated with p-Stat3 pathway. doi:10.1016/j.jns.2013.07.894
Abstract — WCN 2013 No: 1749 Topic: 3 — Stroke Acute stenting in treatment of acute ischemic stroke L. Hajdukovaa, L. Loudovaa, L. Martinkovicb, J. Kasika. aNeurology, Military University Hospital Prague, Czech Republic; bNeurology, 2nd Faculty of Medicine, Charles University Prague, University Hospital Motol, Prague, Czech Republic Background: Acute stenting alone or in combination with other modalities is one possibility for acute stroke treatment in certain indications. Objective: The purpose of our study is to demonstrate the results of acute stenting in 44 patients with acute ischemic stroke during January 2006 and December 2012 in our hospital. Patients and methods: We retrospectively reviewed 44 patients (36 males, 8 females, median 58 years) with acute stroke caused by occlusion or severe stenosis of large artery of anterior (35 patients) or posterior circulation (9 patients). Patients underwent acute stenting alone or in combination with intravenous thrombolysis (IVT), percutaneous angioplasty (PTA), intraarterial thrombolysis (IAT) or mechanical thrombectomy (MT). The procedure was performed within 8 h after stroke onset. We determined median score of National Institute of Health Stroke Scale (NIHSS) at baseline and after 24 h and modified Rankin score (mRS) in 3 months. The Thrombolysis in Myocardial Infarction (TIMI) reperfusion score was assessed by angiography performed immediately after each procedure. Results: The median NIHSS score at baseline was 14 (range 4–25), after 24 h 8 (range 0–35). Good clinical outcome in 3 months (mRS 0–2) had 29 patients (65.9%). The mortality rate was 22.7% (mRS 6). Complete recanalization (TIMI 3) was achieved in 23 patients (52.3%). Symptomatic intracranial hemorrhage occurred in 8 cases (18.0%). Conclusion: Acute stenting has a high recanalization rate. In our study late stent implantation did not show higher risk of complications. doi:10.1016/j.jns.2013.07.895
Abstract — WCN 2013 No: 2132 Topic: 3 — Stroke Peroxynitrite could regulate proliferation and neuronal differentiation of neural stem/progenitor cells through activating WNT/β-catenin signaling pathway J. Shen, X.-M. Chen, T.-T. Yan. School of Chinese Medicine, University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region Background: Hypoxia/ischemia could mediate the differentiation of neural stem/progenitor cells (NSCs) into mature neurons. In hypoxic/ ischemic brain, nitric oxide and superoxide are simultaneously produced and they rapidly react to form peroxynitrite. Whether peroxynitrite can regulate neurogenesis is unknown yet. Objectives: Present study aims to understand the roles of peroxynitrite in regulating self-renewal, proliferation and differentiation of NSCs. Materials and methods: Primary cultured NSCs were subjected to different stimulations including peroxynitrite donor SIN-1, synthesized peroxynitrite and hypoxia treatment. For visualizing the formation of peroxynitrite, we developed a highly sensitive and specific fluorescent
probe and detected the formation of peroxynitrite in the NSCs. We applied different biomarkers including Ki67, BrdU, Tuj1 and DCX, etc. to identify the self-renewal proliferation, and neuronal differentiation of NSCs respectively. Results: Low concentrations of extraneous peroxynitrite (b1 μM) promoted NSC proliferation, self-renewal and neuronal differentiation but high level of peroxynitrite (N5 μM) induced cytotoxicity. Hypoxic treatment induced the production of peroxynitrite and promoted NSC proliferation and self-renewal, and neuronal differentiation. Treatments of peroxynitrite decomposition catalysts (PDCs, FeTMPyP and FeTPPS) reduced hypoxia-induced peroxynitrite formation, NSC proliferation, self-renewal and neuronal differentiation. The neurogenesis promoting effects were partly mediated through activating Wnt/β-catenin signaling pathway. Conclusion: Low concentration of peroxynitrite may serve as a cellular signaling for promoting NSC proliferation, self-renewal and neuronal differentiation. doi:10.1016/j.jns.2013.07.896
Abstract — WCN 2013 No: 2125 Topic: 3 — Stroke Perfusion defect and initial clinical status might predict the poor prognosis of DWI-negative ischemic stroke H.-K. Parka, J.-H. Rhab, S.-B. Koc. aNeurology, Seoul, Republic of Korea; b Inha University Hospital, Incheon, Seoul, Republic of Korea; c Seoul National University Hospital, Seoul, Republic of Korea Purpose: Clinically acute stroke is not always accompanied by positive lesion on the diffusion-weighted image (DWI). In this diffusion negative ischemic stroke (DNIS), the clinical prognosis would be difficult to predict. We investigated which factors are associated with the clinical course in the DNIS. Methods: We retrospectively reviewed data from prospectively collected Inha University Hospital Stroke Registry from January 2007 to December, 2010. We identified and divided the DNIS into two groups, according to the persistence of symptoms after seven days, and analyzed the differences in clinical and imaging factors between two groups. Results: A total of 1398 subjects were enrolled and 142 DNCI patients were identified. Compared to DWI(+) group, the patients with DNIS were younger, and had the low frequency of atrial fibrillation and hypertension. Among DNIS, the patients with fully recovery within seven days, had the lower ABCD2 score, the lower NIHSS on admission, and the lower frequency of perfusion delay on perfusion MRI, compared to the patients with persistent symptoms. In the multivariable analysis, initial NIHSS(≥1) and perfusion delay were independently associated with persistent symptoms (NIHSS, p b 0.001, OR 17.2, 95% CI 5.5–54.0; perfusion delay, p = 0.03, OR 36.7, 95% CI 1.4–977.0). Conclusions: Our results suggested that the perfusion defect and focal neurological deficit might be related to the symptom persistence in the DNIS. doi:10.1016/j.jns.2013.07.897
Abstract — WCN 2013 No: 2108 Topic: 3 — Stroke The sex hormone ratios of acute cerebral infarction K.O. Leea, I.W. Ryooa, K.-Y. Leeb, K.D. Seob, Y.S. Hac, Y.-D. Kima, B. Yoona, S.-J. Naa. aDepartment of Neurology, Konyang University College of Medicine, Daejeon, Republic of Korea;
Abstracts / Journal of the Neurological Sciences 333 (2013) e215–e278
Conclusion: Phosphorylation of Caveolin-1 could attenuate OGDinduced apoptotic cell death in brain microvascular endothelial cells and its mechanisms could be associated with p-Stat3 pathway. doi:10.1016/j.jns.2013.07.894
Abstract — WCN 2013 No: 1749 Topic: 3 — Stroke Acute stenting in treatment of acute ischemic stroke L. Hajdukovaa, L. Loudovaa, L. Martinkovicb, J. Kasika. aNeurology, Military University Hospital Prague, Czech Republic; bNeurology, 2nd Faculty of Medicine, Charles University Prague, University Hospital Motol, Prague, Czech Republic Background: Acute stenting alone or in combination with other modalities is one possibility for acute stroke treatment in certain indications. Objective: The purpose of our study is to demonstrate the results of acute stenting in 44 patients with acute ischemic stroke during January 2006 and December 2012 in our hospital. Patients and methods: We retrospectively reviewed 44 patients (36 males, 8 females, median 58 years) with acute stroke caused by occlusion or severe stenosis of large artery of anterior (35 patients) or posterior circulation (9 patients). Patients underwent acute stenting alone or in combination with intravenous thrombolysis (IVT), percutaneous angioplasty (PTA), intraarterial thrombolysis (IAT) or mechanical thrombectomy (MT). The procedure was performed within 8 h after stroke onset. We determined median score of National Institute of Health Stroke Scale (NIHSS) at baseline and after 24 h and modified Rankin score (mRS) in 3 months. The Thrombolysis in Myocardial Infarction (TIMI) reperfusion score was assessed by angiography performed immediately after each procedure. Results: The median NIHSS score at baseline was 14 (range 4–25), after 24 h 8 (range 0–35). Good clinical outcome in 3 months (mRS 0–2) had 29 patients (65.9%). The mortality rate was 22.7% (mRS 6). Complete recanalization (TIMI 3) was achieved in 23 patients (52.3%). Symptomatic intracranial hemorrhage occurred in 8 cases (18.0%). Conclusion: Acute stenting has a high recanalization rate. In our study late stent implantation did not show higher risk of complications. doi:10.1016/j.jns.2013.07.895
Abstract — WCN 2013 No: 2132 Topic: 3 — Stroke Peroxynitrite could regulate proliferation and neuronal differentiation of neural stem/progenitor cells through activating WNT/β-catenin signaling pathway J. Shen, X.-M. Chen, T.-T. Yan. School of Chinese Medicine, University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region Background: Hypoxia/ischemia could mediate the differentiation of neural stem/progenitor cells (NSCs) into mature neurons. In hypoxic/ ischemic brain, nitric oxide and superoxide are simultaneously produced and they rapidly react to form peroxynitrite. Whether peroxynitrite can regulate neurogenesis is unknown yet. Objectives: Present study aims to understand the roles of peroxynitrite in regulating self-renewal, proliferation and differentiation of NSCs. Materials and methods: Primary cultured NSCs were subjected to different stimulations including peroxynitrite donor SIN-1, synthesized peroxynitrite and hypoxia treatment. For visualizing the formation of peroxynitrite, we developed a highly sensitive and specific fluorescent
probe and detected the formation of peroxynitrite in the NSCs. We applied different biomarkers including Ki67, BrdU, Tuj1 and DCX, etc. to identify the self-renewal proliferation, and neuronal differentiation of NSCs respectively. Results: Low concentrations of extraneous peroxynitrite (b1 μM) promoted NSC proliferation, self-renewal and neuronal differentiation but high level of peroxynitrite (N5 μM) induced cytotoxicity. Hypoxic treatment induced the production of peroxynitrite and promoted NSC proliferation and self-renewal, and neuronal differentiation. Treatments of peroxynitrite decomposition catalysts (PDCs, FeTMPyP and FeTPPS) reduced hypoxia-induced peroxynitrite formation, NSC proliferation, self-renewal and neuronal differentiation. The neurogenesis promoting effects were partly mediated through activating Wnt/β-catenin signaling pathway. Conclusion: Low concentration of peroxynitrite may serve as a cellular signaling for promoting NSC proliferation, self-renewal and neuronal differentiation. doi:10.1016/j.jns.2013.07.896
Abstract — WCN 2013 No: 2125 Topic: 3 — Stroke Perfusion defect and initial clinical status might predict the poor prognosis of DWI-negative ischemic stroke H.-K. Parka, J.-H. Rhab, S.-B. Koc. aNeurology, Seoul, Republic of Korea; b Inha University Hospital, Incheon, Seoul, Republic of Korea; c Seoul National University Hospital, Seoul, Republic of Korea Purpose: Clinically acute stroke is not always accompanied by positive lesion on the diffusion-weighted image (DWI). In this diffusion negative ischemic stroke (DNIS), the clinical prognosis would be difficult to predict. We investigated which factors are associated with the clinical course in the DNIS. Methods: We retrospectively reviewed data from prospectively collected Inha University Hospital Stroke Registry from January 2007 to December, 2010. We identified and divided the DNIS into two groups, according to the persistence of symptoms after seven days, and analyzed the differences in clinical and imaging factors between two groups. Results: A total of 1398 subjects were enrolled and 142 DNCI patients were identified. Compared to DWI(+) group, the patients with DNIS were younger, and had the low frequency of atrial fibrillation and hypertension. Among DNIS, the patients with fully recovery within seven days, had the lower ABCD2 score, the lower NIHSS on admission, and the lower frequency of perfusion delay on perfusion MRI, compared to the patients with persistent symptoms. In the multivariable analysis, initial NIHSS(≥1) and perfusion delay were independently associated with persistent symptoms (NIHSS, p b 0.001, OR 17.2, 95% CI 5.5–54.0; perfusion delay, p = 0.03, OR 36.7, 95% CI 1.4–977.0). Conclusions: Our results suggested that the perfusion defect and focal neurological deficit might be related to the symptom persistence in the DNIS. doi:10.1016/j.jns.2013.07.897
Abstract — WCN 2013 No: 2108 Topic: 3 — Stroke The sex hormone ratios of acute cerebral infarction K.O. Leea, I.W. Ryooa, K.-Y. Leeb, K.D. Seob, Y.S. Hac, Y.-D. Kima, B. Yoona, S.-J. Naa. aDepartment of Neurology, Konyang University College of Medicine, Daejeon, Republic of Korea;