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β-catenin signaling pathway
e310
E-Poster Presentation
Treatment for combined fractures of the mandibular angle and contralateral body or symphysis using resorbable plates R. Yang ∗ , K. Lv, Z. Li, Z. Li Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, China Background: With the development of biodegradable materials, the strength and degradation performances of resorbable plates continue to improve, and resorbable plates are gradually applied in the treatment of midfacial fractures and mandibular fractures. Objectives: To evaluate the results of combined fractures of the mandibular angle and contralateral body or symphysis treated with resorbable plates, and to summarize experiences of the application of resorbable plates. Methods: Eleven patients (5 male and 6 female) with combined fractures of the mandibular angle and contralateral body or symphysis were included in this case series. Open reduction and internal fixation using single or dual resorbable plates by intraoral or extraoral was performed. Postoperatively, follow-up was undertaken to evaluate the fracture healing and the complication of resorbable plates. Findings: Typical granulation tissue and fistula were found around the incision site in two female patients who were treated with single resorbable plate at the mandibular fracture. Other fractures healed without complications during the follow-up period. Conclusions: During the treatment for combined fractures of the mandibular angle and contralateral body or symphysis, the fixation of body or symphysis is prior to the angle. Dual 2.0 mm BioSorb FX resorbable plates fixation of both fracture sites are effective, and resorbable fixation associated with Essig wires are recommended for the fixation of mandibular body or symphysis fractures. In addition, two 2.4 mm BioSorb FX resorbable plates are available for the fixation of mandibular angle fracture when the third molar is extracted. http://dx.doi.org/10.1016/j.ijom.2015.08.392 Platelet-rich fibrin promotes osteogenic differentiation of bone marrow mesenchymal stem cells through canonical Wnt/catenin signaling pathway S. Yang 1,∗ , L. En 1 , G. Yanwei 2 , W. Mingguo 3 1 West China Hospital of Stomatology, Sichuan University, Chengdu, China 2 Jining Hospital of Stomatology, Jining, China 3 Jinan Central Hospital Affiliated to Shandong University, Jinan, China
Background: Platelet-rich fibrin (PRF), a second generation form of platelet-rich concentrate, has been reported to increase bone formation and promote osteogenic differentiation.1 However, the molecular mechanism controlling osteogenesis of PRF has not yet been completely understood. Objectives: In present study, we studied whether PRF could promote osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) through canonical Wnt/-catenin signalling pathway. Methods: Blood samples were collected from the rabbit auricular venous and were prepared for PRF. Rabbit BMSCs were isolated and the flow cytometric analysis was made. Then three passaged BMSCs were cultured in osteogenic medium with or without PRF. In order to research the molecular mechanism controlling osteogenesis of PRF, Dickkopf 1(DKK1), a well
known Wnt/-catenin antagonist, was added in the differentiation medium. After 3 weeks, alizarin red staining and alkaline phosphatase (ALP) assay were examined. The gene expression of osteogenic and adipogenic markers were measured by quantitive real-time PCR (qRT-PCR), and the protein expression of -catenin were detected by western blot analysis. Results: PRF significantly promoted ALP activity; enhanced the mRNA levels of osteogenic markers; and reduced the expression of adipogenic markers. In addition, PRF promoted the expression of -catenin, and DKK1 suppressed PRF-mediated regulation of BMSCs differentiation. Conclusions: Our results suggest that PRF can accelerate osteogenic differentiation of BMSCs through canonical Wnt/catenin signaling pathway.
Reference Choukroun, J., Diss, A., Simonpieri, A., et al. (2006). Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: Clinical effects on tissue healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 101(3), e56–e60.
http://dx.doi.org/10.1016/j.ijom.2015.08.393 Pilot study of donor site harvesting with a piezo device to improve tooth autotransplantation G.K. Sándor 1,2 , L.P. Ylikontiola 1,∗ 1 Oral and Maxillofacial Surgery, Oulu University Hospital, University of Oulu, Oulu, Finland 2 BioMediTech, Institute of Biomedical Technology, University of Tampere, Tampere, Finland
Background: Piezo-surgery has been successfully applied at our centre for harvesting autogenous bone grafts from the iliac crest with a reduction in donor site morbidity. Piezo-surgery has been adapted to intraoral use and is routine in sinus lift surgery for example. Candidate autotransplant donor teeth are often ectopically positioned and may require a considerable amount of potentially heat generating alveolar bone drilling to allow removal of the tooth. Objectives: This study aimed to apply piezo-surgery to the donor site harvesting of unerupted permanent premolar teeth for autotransplantation. Methods: Twenty permanent maxillary or mandibular premolar teeth with sufficient crown and root development, intact dental follicles and which were morphologically sound were designated as potential candidate teeth for autotransplantation during multidisciplinary surgical and orthodontic consultations. A piezosurgical device was used to remove sufficient alveolar bone from the pericoronal region to allow atraumatic delivery of the tooth with an intact follicle. These teeth were then transplanted to their recipient beds once these were prepared. The development of the transplanted teeth was followed clinically and radiographically for the next 12 months. Findings: All 20 transplanted teeth healed in a satisfactory manner without excessive mobility or ankylosis. Although the root lengths were variable, all 20 teeth were morphologically intact and clinically sound. Conclusions: Piezo-surgical harvesting of donor autotransplant teeth results in well prepared dental units capable of becoming functional transplanted teeth. http://dx.doi.org/10.1016/j.ijom.2015.08.394
E-Poster Presentation
Treatment for combined fractures of the mandibular angle and contralateral body or symphysis using resorbable plates R. Yang ∗ , K. Lv, Z. Li, Z. Li Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, China Background: With the development of biodegradable materials, the strength and degradation performances of resorbable plates continue to improve, and resorbable plates are gradually applied in the treatment of midfacial fractures and mandibular fractures. Objectives: To evaluate the results of combined fractures of the mandibular angle and contralateral body or symphysis treated with resorbable plates, and to summarize experiences of the application of resorbable plates. Methods: Eleven patients (5 male and 6 female) with combined fractures of the mandibular angle and contralateral body or symphysis were included in this case series. Open reduction and internal fixation using single or dual resorbable plates by intraoral or extraoral was performed. Postoperatively, follow-up was undertaken to evaluate the fracture healing and the complication of resorbable plates. Findings: Typical granulation tissue and fistula were found around the incision site in two female patients who were treated with single resorbable plate at the mandibular fracture. Other fractures healed without complications during the follow-up period. Conclusions: During the treatment for combined fractures of the mandibular angle and contralateral body or symphysis, the fixation of body or symphysis is prior to the angle. Dual 2.0 mm BioSorb FX resorbable plates fixation of both fracture sites are effective, and resorbable fixation associated with Essig wires are recommended for the fixation of mandibular body or symphysis fractures. In addition, two 2.4 mm BioSorb FX resorbable plates are available for the fixation of mandibular angle fracture when the third molar is extracted. http://dx.doi.org/10.1016/j.ijom.2015.08.392 Platelet-rich fibrin promotes osteogenic differentiation of bone marrow mesenchymal stem cells through canonical Wnt/catenin signaling pathway S. Yang 1,∗ , L. En 1 , G. Yanwei 2 , W. Mingguo 3 1 West China Hospital of Stomatology, Sichuan University, Chengdu, China 2 Jining Hospital of Stomatology, Jining, China 3 Jinan Central Hospital Affiliated to Shandong University, Jinan, China
Background: Platelet-rich fibrin (PRF), a second generation form of platelet-rich concentrate, has been reported to increase bone formation and promote osteogenic differentiation.1 However, the molecular mechanism controlling osteogenesis of PRF has not yet been completely understood. Objectives: In present study, we studied whether PRF could promote osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) through canonical Wnt/-catenin signalling pathway. Methods: Blood samples were collected from the rabbit auricular venous and were prepared for PRF. Rabbit BMSCs were isolated and the flow cytometric analysis was made. Then three passaged BMSCs were cultured in osteogenic medium with or without PRF. In order to research the molecular mechanism controlling osteogenesis of PRF, Dickkopf 1(DKK1), a well
known Wnt/-catenin antagonist, was added in the differentiation medium. After 3 weeks, alizarin red staining and alkaline phosphatase (ALP) assay were examined. The gene expression of osteogenic and adipogenic markers were measured by quantitive real-time PCR (qRT-PCR), and the protein expression of -catenin were detected by western blot analysis. Results: PRF significantly promoted ALP activity; enhanced the mRNA levels of osteogenic markers; and reduced the expression of adipogenic markers. In addition, PRF promoted the expression of -catenin, and DKK1 suppressed PRF-mediated regulation of BMSCs differentiation. Conclusions: Our results suggest that PRF can accelerate osteogenic differentiation of BMSCs through canonical Wnt/catenin signaling pathway.
Reference Choukroun, J., Diss, A., Simonpieri, A., et al. (2006). Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: Clinical effects on tissue healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 101(3), e56–e60.
http://dx.doi.org/10.1016/j.ijom.2015.08.393 Pilot study of donor site harvesting with a piezo device to improve tooth autotransplantation G.K. Sándor 1,2 , L.P. Ylikontiola 1,∗ 1 Oral and Maxillofacial Surgery, Oulu University Hospital, University of Oulu, Oulu, Finland 2 BioMediTech, Institute of Biomedical Technology, University of Tampere, Tampere, Finland
Background: Piezo-surgery has been successfully applied at our centre for harvesting autogenous bone grafts from the iliac crest with a reduction in donor site morbidity. Piezo-surgery has been adapted to intraoral use and is routine in sinus lift surgery for example. Candidate autotransplant donor teeth are often ectopically positioned and may require a considerable amount of potentially heat generating alveolar bone drilling to allow removal of the tooth. Objectives: This study aimed to apply piezo-surgery to the donor site harvesting of unerupted permanent premolar teeth for autotransplantation. Methods: Twenty permanent maxillary or mandibular premolar teeth with sufficient crown and root development, intact dental follicles and which were morphologically sound were designated as potential candidate teeth for autotransplantation during multidisciplinary surgical and orthodontic consultations. A piezosurgical device was used to remove sufficient alveolar bone from the pericoronal region to allow atraumatic delivery of the tooth with an intact follicle. These teeth were then transplanted to their recipient beds once these were prepared. The development of the transplanted teeth was followed clinically and radiographically for the next 12 months. Findings: All 20 transplanted teeth healed in a satisfactory manner without excessive mobility or ankylosis. Although the root lengths were variable, all 20 teeth were morphologically intact and clinically sound. Conclusions: Piezo-surgical harvesting of donor autotransplant teeth results in well prepared dental units capable of becoming functional transplanted teeth. http://dx.doi.org/10.1016/j.ijom.2015.08.394