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Prevalence of autoantibodies in Egyptian patients with chronic hepatitis C related liver disease
Immunology, autoimmune liver disease
I c09 05 I N E U T R O P H I L DEFENSIN H N P IN B L O O D FROM PATIENTS W I T H CHRONIC AND ACUTE HEPATITIS A. Klvuchareva, N. Petyovka, N. Voitenok Department of Infectious Diseases, Postgraduate Medical Institute, Minsk, Institute of Haematology, Minsk, Belarus, Fund for Molecular Hematology & Immunology, Moscow, Russia.
Bactericidal cationic peptides c¢-defensins are stored in granules of neutrophils and released upon activation by exocytosis.We studied the levels of neutrophil defensins I-INP in plasma from patients with CHC, CHB, mixed VH: B+C+D, acute HB and acute HC. HNP ELISA was performed using monoelonal antibodies to human alpha defensin HNP. Plasma samples from healthy donors contained less that 50 ng/ml of HNP. Hepatitis was accompanied by elevated levels of plasma defensin: acute VH - 326 + 139 ng/ml and CVH - 327 a: 289 ng/ml. We found no difference between the levels of plasma defensin in patients with HBV-, HCV-, HBV+HDV+HCVinfection (p>0,1). Highest levels of defensins (>600 ng/ml) were detected in patients with severe course of hepatitis and hepatic failure, middle concentrations of defensins (151 - 600 ng/ml) were detected in patients with severe and mild morphologic activity of CH, the lowest defensins levels (50 -150 ng/ml) - in patients with mild and minimal activity. The data show that acute and chronic VH are accompanied by elevated levels of plasma defensins and high levels of defensins correlated with severity of the disease.
C09/06
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C09/07
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PREVALENCE OF AUTOANTIBODIES IN EGYPTIAN PATIENTS W I T H CHRONIC HEPATITIS C RELATED LIVER DISEASE A.M. E1-Sahly i, M. Khamis 1, M. E1-Razik¥ i, W. El-Metnawi 2 1Department of Tropical Medicine, Faculty of Medicine, Cairo, Egypt. 2Department of Cancer Biology Kasr E1-Eini, Faculty of Medicine, Cairo, Egypt. Hepatitis C is amen8 the most common causes of chronic liver disease worldwide. The i~evalence of hepatitis C infection is high in Egypt, most of the palients proceed to chtcoieity. HCV infection may interface with pretenses considered to be autdimmurt¢in nature whether through the ©x[a'useionof oxtrahepatic mamfestafionsor the formation of autoantilxxlies. The aim of this work was to detecl the pt'evalen¢,¢of autoanfibodies in chronic hepaliUscaused by isolated HCV infe,.¢tionand to study their impact on the ¢lini©al course of the disease. We studied 38 Egyp6au patieals with HCV related chronic liver disease by clinical, laboratory and histopathologicalasse~smantwith determinationof semra titre of A~A, ASMA, AMA. LKM1 antibody and APCA using Indir~t Immar~fluorcscma:~for patients as well as a group of hesRhycontrols (25 subjects). Many patients had high titr~s of aatmmtibodiesas well as diffeaent combinationsof the tested antibodies. No significant d i ~ was found when these levels were correlated wah ALT, AST, ALP, ESIL Hb or ste8e and grade of the disease as revealed in fiver biopsy. ASMA ANA
Positivity% 94.73 36.80
AMA
73.90
LKM1 Ab 63.20 APCA
18.42
1/60 39.47 26.32 36.84 5000 18.42
11120 7.80
1/360 34.21 2,63
21.05
15.79
7.89 0.00
5.26 0.00
21.05
Autoaatibodies a r e frequentlyencoun~red in HCV related CLD but they don't alter the clinical oonrse.This high prevalenceof autoantibodiesmay explain the low response rate to interferontherapy in Egyptian lmtientswith HCV.
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co,,o
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PREVALENCE OF SEROLOGICAL MARKERS OF AUTOIMMUNITY IN GREEK DRUG ABUSERS W I T H CHRONIC HEPATITIS C St. Kanatakis 1, A. Georgiou l, E Tserp¢ 1, P. Houpas l, E. Kresmatinou 2, I. Theodoropoulou 2, D. Adracta 3, K. Terzo~lou 2 13rd Medical Department, Hellenic CrossHospital, Athens. 2Department of Immunology, Hellenic Cross Hospital, Athens. 3Department of Blood Transfusion, Hellenic Cross Hospital, Athens.
THE FEATURES OF A U T O I M M U N E C H O L A N G I T I S S. Kacar, Y. Ko~ar, E. Parlak, E. Erden l, N. Sasmaz, E Oguz, S. Disibeyaz, H. Alklm Gastroenterology Unit of Ankara Yuksek Ihtlsas Hospital, Ankara, Turkey. 1Pathology Department of Medical School of Ankara University, Ankara, Turkey.
The presence of non-organ specific autoantibodies associated with chronic hepatitis C, is occurring all over the world with variable frequency. Aim: To investigate the prevalence of this association in Greek drug abusers and estimate possible clinical differences between pts with or without autoimmune markers as well. Patients: 210 consecutive dr.ab, pts (180 men and 30 women mean age 30,4 yrs), were enrolled in this prospective study that covered the period 1995-98. Their sera screened for the presence of a) HBV markers b) Anti-HCV c) ANA, SMA, AMA, anti-LKM d) Cryoglobulins e) Ra-test f) Anti-thyroid antibodies. Results: ANA in titer 1:40-1:640 showed 29(17%) out of 170 anti-HCV positive pts, 8 of whom were also anti-Hbs, anti-Hbc positive. SMA in titer 1:20-1:320 showed 29 (17%), 11 of whom were also anti-Fibs, Anti-Hbc positive. MC type II and I~ asymptomatic showed 8 (7,6%) out of 105 pts, while Ra-test 12(9,2%) out of 130. Anti-LKM and anti-thyroid were extremely rare. Conclusions: Our pts developed non-organ specific autoantibodies in lower rates compared with other series. The presence of these autoantibodies does not appear to be related with the severity of infection.
d-da,g~ . ~ a - ~ . . ' ~ a~xxty (ANA)ar~or~ x ~ r~ ~O,~a ~ S M A ) but vdfm , , ~ ~ a~ (~_A~ We d e s ~ ~x ~ r r ~ w~h = ~ i c ~ of l/gh ~ ard C~T hers and ~ se~g~nmm~gno~eraioi~ lVl~a~ ANA, SMA, AMA md Ix~IC_A v0ae ~ wi~ i . . ~ ~ axt ~ anfigms ~ react ag~st ~ with ~A. ~ e ~ ~'~esv~ae ~aledbyomtm'xfi:~t R m l ~ Tyrol ~x pelkxlshad evidmtcinix~ wilh~ azl vatkesm acknis~rt The ether4 rni~s had p , , ~ ard ~d~inga r d ~ a~e ~ =xt C,GT L-~tsw~e r~h (rredm]294U/Lwd 423U/L~ r e a i ~ ) AM& ar~ M2 and ~ v~re a~ ~ was ~ ~ i ~ i h aliens= withti~ 1/80-1/320.Two cases haclSMA ~ w i ~ 1/80titus Re~d~ ofERCP ixzExn~ t0 4 p a n s v~e r , m ~ ~ g i ~ r~ae x . ~ ~ t m t a ~ and pa¢ k~,ia-,~n, lmlingplaledesmxtim,flxcKsard d,_~_~ bff~ ~ . Four =~esl'ed ~ifmr~ ~=~se ~ ~ 1 ard ~ i / o d m m ~ t,at ci~m~m p ~ [
Cr~l,~m: AIC hasa d / i ~ poflc ~
n
~
219
I c09 05 I N E U T R O P H I L DEFENSIN H N P IN B L O O D FROM PATIENTS W I T H CHRONIC AND ACUTE HEPATITIS A. Klvuchareva, N. Petyovka, N. Voitenok Department of Infectious Diseases, Postgraduate Medical Institute, Minsk, Institute of Haematology, Minsk, Belarus, Fund for Molecular Hematology & Immunology, Moscow, Russia.
Bactericidal cationic peptides c¢-defensins are stored in granules of neutrophils and released upon activation by exocytosis.We studied the levels of neutrophil defensins I-INP in plasma from patients with CHC, CHB, mixed VH: B+C+D, acute HB and acute HC. HNP ELISA was performed using monoelonal antibodies to human alpha defensin HNP. Plasma samples from healthy donors contained less that 50 ng/ml of HNP. Hepatitis was accompanied by elevated levels of plasma defensin: acute VH - 326 + 139 ng/ml and CVH - 327 a: 289 ng/ml. We found no difference between the levels of plasma defensin in patients with HBV-, HCV-, HBV+HDV+HCVinfection (p>0,1). Highest levels of defensins (>600 ng/ml) were detected in patients with severe course of hepatitis and hepatic failure, middle concentrations of defensins (151 - 600 ng/ml) were detected in patients with severe and mild morphologic activity of CH, the lowest defensins levels (50 -150 ng/ml) - in patients with mild and minimal activity. The data show that acute and chronic VH are accompanied by elevated levels of plasma defensins and high levels of defensins correlated with severity of the disease.
C09/06
]
C09/07
]
PREVALENCE OF AUTOANTIBODIES IN EGYPTIAN PATIENTS W I T H CHRONIC HEPATITIS C RELATED LIVER DISEASE A.M. E1-Sahly i, M. Khamis 1, M. E1-Razik¥ i, W. El-Metnawi 2 1Department of Tropical Medicine, Faculty of Medicine, Cairo, Egypt. 2Department of Cancer Biology Kasr E1-Eini, Faculty of Medicine, Cairo, Egypt. Hepatitis C is amen8 the most common causes of chronic liver disease worldwide. The i~evalence of hepatitis C infection is high in Egypt, most of the palients proceed to chtcoieity. HCV infection may interface with pretenses considered to be autdimmurt¢in nature whether through the ©x[a'useionof oxtrahepatic mamfestafionsor the formation of autoantilxxlies. The aim of this work was to detecl the pt'evalen¢,¢of autoanfibodies in chronic hepaliUscaused by isolated HCV infe,.¢tionand to study their impact on the ¢lini©al course of the disease. We studied 38 Egyp6au patieals with HCV related chronic liver disease by clinical, laboratory and histopathologicalasse~smantwith determinationof semra titre of A~A, ASMA, AMA. LKM1 antibody and APCA using Indir~t Immar~fluorcscma:~for patients as well as a group of hesRhycontrols (25 subjects). Many patients had high titr~s of aatmmtibodiesas well as diffeaent combinationsof the tested antibodies. No significant d i ~ was found when these levels were correlated wah ALT, AST, ALP, ESIL Hb or ste8e and grade of the disease as revealed in fiver biopsy. ASMA ANA
Positivity% 94.73 36.80
AMA
73.90
LKM1 Ab 63.20 APCA
18.42
1/60 39.47 26.32 36.84 5000 18.42
11120 7.80
1/360 34.21 2,63
21.05
15.79
7.89 0.00
5.26 0.00
21.05
Autoaatibodies a r e frequentlyencoun~red in HCV related CLD but they don't alter the clinical oonrse.This high prevalenceof autoantibodiesmay explain the low response rate to interferontherapy in Egyptian lmtientswith HCV.
[
co,,o
]
PREVALENCE OF SEROLOGICAL MARKERS OF AUTOIMMUNITY IN GREEK DRUG ABUSERS W I T H CHRONIC HEPATITIS C St. Kanatakis 1, A. Georgiou l, E Tserp¢ 1, P. Houpas l, E. Kresmatinou 2, I. Theodoropoulou 2, D. Adracta 3, K. Terzo~lou 2 13rd Medical Department, Hellenic CrossHospital, Athens. 2Department of Immunology, Hellenic Cross Hospital, Athens. 3Department of Blood Transfusion, Hellenic Cross Hospital, Athens.
THE FEATURES OF A U T O I M M U N E C H O L A N G I T I S S. Kacar, Y. Ko~ar, E. Parlak, E. Erden l, N. Sasmaz, E Oguz, S. Disibeyaz, H. Alklm Gastroenterology Unit of Ankara Yuksek Ihtlsas Hospital, Ankara, Turkey. 1Pathology Department of Medical School of Ankara University, Ankara, Turkey.
The presence of non-organ specific autoantibodies associated with chronic hepatitis C, is occurring all over the world with variable frequency. Aim: To investigate the prevalence of this association in Greek drug abusers and estimate possible clinical differences between pts with or without autoimmune markers as well. Patients: 210 consecutive dr.ab, pts (180 men and 30 women mean age 30,4 yrs), were enrolled in this prospective study that covered the period 1995-98. Their sera screened for the presence of a) HBV markers b) Anti-HCV c) ANA, SMA, AMA, anti-LKM d) Cryoglobulins e) Ra-test f) Anti-thyroid antibodies. Results: ANA in titer 1:40-1:640 showed 29(17%) out of 170 anti-HCV positive pts, 8 of whom were also anti-Hbs, anti-Hbc positive. SMA in titer 1:20-1:320 showed 29 (17%), 11 of whom were also anti-Fibs, Anti-Hbc positive. MC type II and I~ asymptomatic showed 8 (7,6%) out of 105 pts, while Ra-test 12(9,2%) out of 130. Anti-LKM and anti-thyroid were extremely rare. Conclusions: Our pts developed non-organ specific autoantibodies in lower rates compared with other series. The presence of these autoantibodies does not appear to be related with the severity of infection.
d-da,g~ . ~ a - ~ . . ' ~ a~xxty (ANA)ar~or~ x ~ r~ ~O,~a ~ S M A ) but vdfm , , ~ ~ a~ (~_A~ We d e s ~ ~x ~ r r ~ w~h = ~ i c ~ of l/gh ~ ard C~T hers and ~ se~g~nmm~gno~eraioi~ lVl~a~ ANA, SMA, AMA md Ix~IC_A v0ae ~ wi~ i . . ~ ~ axt ~ anfigms ~ react ag~st ~ with ~A. ~ e ~ ~'~esv~ae ~aledbyomtm'xfi:~t R m l ~ Tyrol ~x pelkxlshad evidmtcinix~ wilh~ azl vatkesm acknis~rt The ether4 rni~s had p , , ~ ard ~d~inga r d ~ a~e ~ =xt C,GT L-~tsw~e r~h (rredm]294U/Lwd 423U/L~ r e a i ~ ) AM& ar~ M2 and ~ v~re a~ ~ was ~ ~ i ~ i h aliens= withti~ 1/80-1/320.Two cases haclSMA ~ w i ~ 1/80titus Re~d~ ofERCP ixzExn~ t0 4 p a n s v~e r , m ~ ~ g i ~ r~ae x . ~ ~ t m t a ~ and pa¢ k~,ia-,~n, lmlingplaledesmxtim,flxcKsard d,_~_~ bff~ ~ . Four =~esl'ed ~ifmr~ ~=~se ~ ~ 1 ard ~ i / o d m m ~ t,at ci~m~m p ~ [
Cr~l,~m: AIC hasa d / i ~ poflc ~
n
~
219