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Prevalence of human papillomavirus type 16 and 18 co-infection in South Korean women
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Abstracts / Journal of Clinical Virology 70 (2015) S1–S126
by an average of 24 paediatricians (24/80, 29%). Six hundred and seventy-eight children were recruited; EV infection was diagnosed in 530/673 (78.7%). Two epidemic waves were detected, from week 25 to week 27 and in Mid-October. A definite EV type was identified in 527/530 (99.4%) patients. Coxsackievirus A6 accounted for 54% (286/530), followed by CV-A16 (123/530, 23%). Only 6 EV-A71 (1.1%) infections were detected. No neurological complication was reported. CV-A6 infections were more frequently associated with eruptions extending to the upper and/or lower limbs in 143/286 (50%) of children and to the face in 133/286 (43.5%) than other EV-A types. Conclusions: This sentinel surveillance system for HFMD/HA was feasible and effective at a national scale in detecting epidemic peaks and, in combination with virological investigation, in identifying EV types associated with HFMD outbreaks. In the future, it should be able to provide the health authorities with accurate information on epidemiological trends of HFMD and allow early detection of epidemics or upsurge of EV-A71 infections. http://dx.doi.org/10.1016/j.jcv.2015.07.096 Abstract No: 1547 Presentation at ESCV 2015: Poster 1 Prevalence of human papillomavirus type 16 and 18 co-infection in South Korean women Hyewon Park ∗ , Hyoeun Shim, Jung Eun Choi, Kyoung Ho Roh Seegene Medical Foundation, Seoul, South Korea Background: Cervical cancer is the seventh common cancer among South Korean women. The aim of this study is to provide new insights into infection patterns of two most frequent highrisk human papillomavirus (HR-HPV-16 and -18) and coinfection in cervical samples of South Korean population. Methods: Twenty-one thousand eight hundred twenty-two cervical swab specimens were collected from January 2015 to April 2015. HPV 16, HPV 18 and other HR-HPV types were detected simultaneously using multiplex commercial real-time polymerase chain reaction kit (Seegene, South Korea). Results: Of 21,822 cervical samples, 13,936 were negative for HR-HPV (63.9%). Of HR-HPV positive specimens, 736 were HPV16 positive (9.3%) and 255 were HPV-18 positive (3.2%). Two hundred sixty-nine of HPV-16(+) specimens and 78 of HPV-18(+) were mixed infection of HR-HPV (36.5% and 30.6%, respectively). Twenty-five specimens showed HPV-16 and -18 co-infection (0.3%). In HPV-16(+) and -18(+) patients, HPV-16/18 co-infection ratio were 3.4% and 9.8%, respectively. There were tendency of positive correlation between HPV viral load and HR-HPV co-infection ratio. The number of infected genotypes was related to HPV viral load. But both were not statistically significant (OR = 2.3, P > 0.05 and OR = 1.8, P > 0.05, respectively). Conclusions: HPV-16/18 co-infection was common in HRHPV(+) specimens. This finding implies an accelerated progression to cervical neoplasia. Data suggest that an association between HRHPV co-infection ratio and lifetime number of sexual partners. http://dx.doi.org/10.1016/j.jcv.2015.07.097
Abstract No: 1549 Presentation at ESCV 2015: Poster 1 Occurrence of human parvovirus B19 and bocavirus 1–4 in healthy and diseased gut M. Xu ∗ , M. Toppinen, K. Taina Sipponen Virology, University of Helsinki and HUCH Abdominal Center, Helsinki University Hospital, Helsinki, Finland Background: Occurrence of human parvovirus B19 and bocavirus 1–4 in healthy and diseased gut Human bocaviruses and parvovirus B19 are two human-pathogenic parvoviruses, which associate with a wide range of clinical diseases. HBoV1 causes mild to severe respiratory disease mainly in children, and it has sporadically been detected in healthy tonsils of children, but also tissue biopsies from adult lung and colorectal tumors. HBoV2-4 are mainly enteric, and HBoV3 has been detected in a gut biopsy. Human parvovirus B19 causes rash, arthropaties, anemias and fetal death, and has been suggested to associate with inflammatory bowel disease (IBD). The aim of this study was to determine the occurrence of active parvovirus infection or passive genome persistence in the gastrointestinal tract of patients with chronic diseases and of healthy subjects, and its association with adenomatous polyps, IBD and malignant tumors. Methods: To investigate the presence of parvoviruses, DNA was extracted by Qiagen kits from 89 fresh-frozen intestinal biopsy specimens collected from patients with polyps, tumors or IBD, and from healthy controls. In-house real-time multiplex HBoV1–4 qPCR and Pan-B19 genotype qPCR, were applied to detect and quantify the presence of HBoV1–4 and B19V in the specimens. PCR products were subjected to gene sequencing analysis to confirm the amplification results and the virus subtype. Results: Of the 89 fresh-frozen intestinal biopsies from 61 subjects, 15 (16.85%) were positive for B19V in patients with polyps, inactive IBD, and in healthy biopsies. However, the B19 viral loads were very low, with <10 copies/l. Specifically, 1/9 polyp biopsies and 1/8 inactive colitis were B19V positive, but all of the active colitis biopsies were negative. A higher presence of B19V was shown in healthy biopsies, 13/68 cases. No HBoV DNA was found in either cohort. Conclusions: No human bocavirus DNA was detected, whereas parvovirus B19 DNA was identified in 9.5% of intestinal biopsies from polyps, tumors, or IBD, and in 19.1% of nondiseased tissues. The presence of virus DNA does not associate B19V with polyps or IBD, due to an even higher rate of virus in the healthy control biopsies. We will continue with the rest of the similar biopsies and include also more tumorous tissues to clarify whether these viruses are oncolytic, oncogenic or merely bystanders. http://dx.doi.org/10.1016/j.jcv.2015.07.098 Abstract No: 1551 Presentation at ESCV 2015: Poster 1 Influenza epidemiology and circulating variants in N. Greece, 2014–15 M. Exindari ∗ , A. Melidou, G. Gioula, N. Malisiovas Aristotle University of Thessaloniki, Greece Background: Influenza needs constant surveillance and alert since it is a global and continuous health threat, causing yearly local outbreaks, epidemics or even pandemics. A(H3N2) and B influenza variants circulated during the 2014–15 season, an Therefore an epi-
Abstracts / Journal of Clinical Virology 70 (2015) S1–S126
by an average of 24 paediatricians (24/80, 29%). Six hundred and seventy-eight children were recruited; EV infection was diagnosed in 530/673 (78.7%). Two epidemic waves were detected, from week 25 to week 27 and in Mid-October. A definite EV type was identified in 527/530 (99.4%) patients. Coxsackievirus A6 accounted for 54% (286/530), followed by CV-A16 (123/530, 23%). Only 6 EV-A71 (1.1%) infections were detected. No neurological complication was reported. CV-A6 infections were more frequently associated with eruptions extending to the upper and/or lower limbs in 143/286 (50%) of children and to the face in 133/286 (43.5%) than other EV-A types. Conclusions: This sentinel surveillance system for HFMD/HA was feasible and effective at a national scale in detecting epidemic peaks and, in combination with virological investigation, in identifying EV types associated with HFMD outbreaks. In the future, it should be able to provide the health authorities with accurate information on epidemiological trends of HFMD and allow early detection of epidemics or upsurge of EV-A71 infections. http://dx.doi.org/10.1016/j.jcv.2015.07.096 Abstract No: 1547 Presentation at ESCV 2015: Poster 1 Prevalence of human papillomavirus type 16 and 18 co-infection in South Korean women Hyewon Park ∗ , Hyoeun Shim, Jung Eun Choi, Kyoung Ho Roh Seegene Medical Foundation, Seoul, South Korea Background: Cervical cancer is the seventh common cancer among South Korean women. The aim of this study is to provide new insights into infection patterns of two most frequent highrisk human papillomavirus (HR-HPV-16 and -18) and coinfection in cervical samples of South Korean population. Methods: Twenty-one thousand eight hundred twenty-two cervical swab specimens were collected from January 2015 to April 2015. HPV 16, HPV 18 and other HR-HPV types were detected simultaneously using multiplex commercial real-time polymerase chain reaction kit (Seegene, South Korea). Results: Of 21,822 cervical samples, 13,936 were negative for HR-HPV (63.9%). Of HR-HPV positive specimens, 736 were HPV16 positive (9.3%) and 255 were HPV-18 positive (3.2%). Two hundred sixty-nine of HPV-16(+) specimens and 78 of HPV-18(+) were mixed infection of HR-HPV (36.5% and 30.6%, respectively). Twenty-five specimens showed HPV-16 and -18 co-infection (0.3%). In HPV-16(+) and -18(+) patients, HPV-16/18 co-infection ratio were 3.4% and 9.8%, respectively. There were tendency of positive correlation between HPV viral load and HR-HPV co-infection ratio. The number of infected genotypes was related to HPV viral load. But both were not statistically significant (OR = 2.3, P > 0.05 and OR = 1.8, P > 0.05, respectively). Conclusions: HPV-16/18 co-infection was common in HRHPV(+) specimens. This finding implies an accelerated progression to cervical neoplasia. Data suggest that an association between HRHPV co-infection ratio and lifetime number of sexual partners. http://dx.doi.org/10.1016/j.jcv.2015.07.097
Abstract No: 1549 Presentation at ESCV 2015: Poster 1 Occurrence of human parvovirus B19 and bocavirus 1–4 in healthy and diseased gut M. Xu ∗ , M. Toppinen, K. Taina Sipponen Virology, University of Helsinki and HUCH Abdominal Center, Helsinki University Hospital, Helsinki, Finland Background: Occurrence of human parvovirus B19 and bocavirus 1–4 in healthy and diseased gut Human bocaviruses and parvovirus B19 are two human-pathogenic parvoviruses, which associate with a wide range of clinical diseases. HBoV1 causes mild to severe respiratory disease mainly in children, and it has sporadically been detected in healthy tonsils of children, but also tissue biopsies from adult lung and colorectal tumors. HBoV2-4 are mainly enteric, and HBoV3 has been detected in a gut biopsy. Human parvovirus B19 causes rash, arthropaties, anemias and fetal death, and has been suggested to associate with inflammatory bowel disease (IBD). The aim of this study was to determine the occurrence of active parvovirus infection or passive genome persistence in the gastrointestinal tract of patients with chronic diseases and of healthy subjects, and its association with adenomatous polyps, IBD and malignant tumors. Methods: To investigate the presence of parvoviruses, DNA was extracted by Qiagen kits from 89 fresh-frozen intestinal biopsy specimens collected from patients with polyps, tumors or IBD, and from healthy controls. In-house real-time multiplex HBoV1–4 qPCR and Pan-B19 genotype qPCR, were applied to detect and quantify the presence of HBoV1–4 and B19V in the specimens. PCR products were subjected to gene sequencing analysis to confirm the amplification results and the virus subtype. Results: Of the 89 fresh-frozen intestinal biopsies from 61 subjects, 15 (16.85%) were positive for B19V in patients with polyps, inactive IBD, and in healthy biopsies. However, the B19 viral loads were very low, with <10 copies/l. Specifically, 1/9 polyp biopsies and 1/8 inactive colitis were B19V positive, but all of the active colitis biopsies were negative. A higher presence of B19V was shown in healthy biopsies, 13/68 cases. No HBoV DNA was found in either cohort. Conclusions: No human bocavirus DNA was detected, whereas parvovirus B19 DNA was identified in 9.5% of intestinal biopsies from polyps, tumors, or IBD, and in 19.1% of nondiseased tissues. The presence of virus DNA does not associate B19V with polyps or IBD, due to an even higher rate of virus in the healthy control biopsies. We will continue with the rest of the similar biopsies and include also more tumorous tissues to clarify whether these viruses are oncolytic, oncogenic or merely bystanders. http://dx.doi.org/10.1016/j.jcv.2015.07.098 Abstract No: 1551 Presentation at ESCV 2015: Poster 1 Influenza epidemiology and circulating variants in N. Greece, 2014–15 M. Exindari ∗ , A. Melidou, G. Gioula, N. Malisiovas Aristotle University of Thessaloniki, Greece Background: Influenza needs constant surveillance and alert since it is a global and continuous health threat, causing yearly local outbreaks, epidemics or even pandemics. A(H3N2) and B influenza variants circulated during the 2014–15 season, an Therefore an epi-