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PREVALENCE OF ORAL LESIONS IN INDIVIDUALS UP TO 19 YEARS OF AGE: ANALYSIS OF BIOPSIED CASES FROM A SINGLE INSTITUTION
ABSTRACTS
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IMMUNOHISTOCHEMICAL COMPARATIVE STUDY OF PERIPHERAL, PERI-IMPLANT, AND CENTRAL GIANT CELL LESIONS. FABIO RAMOA PIRES, THAYNA MELO DE LIMA MORAIS, CIRO DANTAS SOARES, JOSE MANUEL AGUIRRE URIZAR, JAVIER ALBERDI NAVARRO and, OSLEI PAES DE ALMEIDA Central giant cell lesions (CGCL) and peripheral giant cell lesions (PGCL) are reactive lesions of unknown etiopathogenesis and similar histologic features. PGCL affects the gingiva and alveolar mucosa, but some rare cases can be associated with dental implants. Objective: In the present study, we compared the expression of smooth muscle actin, Bcl-2, GLUT-1, CD68, OPG, RANK, Ki67, and CD34 in a series of CGCL and conventional and periimplant PGCL. Study Design: Clinical data were retrieved from the laboratory records and histologic analysis was performed in hematoxylin and eosin (H&E)-stained sections. Immunohistochemical reactions for detecting the above mentioned antibodies were performed and digitally scored. Results: The digital scores for CD68 and Bcl-2 were higher in conventional PGCL and CGCL than in peri-implant PGCL (P = .033 for CD68 and P <.0001 for Bcl-2). Microvessel density was higher in the conventional PGCL than in CGCL and periimplant PGCL (P = .002). Conclusions: The current results demonstrated some differences in microvessel density, proliferative activity, and expression of CD68 and Bcl-2 among conventional PGCL, periimplant PGCL and CGCL. Further studies are encouraged to better understanding of these early findings.
COMPARATIVE STUDY AMONG HISTOLOGIC GRADUATION SYSTEMS OF ORAL EPITHELIAL DYSPLASIAS. JULIANA COSTA DE OLIVEIRA, GABRIELA MEDEIROS DE CERQUEIRA, e JOAO FIGUEIRA SCARINI, PRISCILLA RODRIGUES ^ CAMARA, REBECA DE SOUZA AZEVEDO, DECIO DOS SANTOS PINTO JUNIOR and, RENATA TUCCI Objective: The objective of this study was to evaluate the histologic grading systems for oral epithelial dysplasia (ODE). Study Design: One hundred slides of ODE containing cases selected from 2 distinct oral pathology services were examined by 4 evaluators, who classified each case according to the architectural and cytologic criteria and were diagnosed according to the World Health Organization (WHO) system and binary. In addition to these 2 systems, a third form of analysis was considered, taking into account the presence or absence of ODE. Results: The results were statistically analyzed in a descriptive way and by the correlation methods (Kendall tau-B and Phi test) and concordance (kappa). The WHO graduation system presented lower agreement among the evaluators; the best agreement among the evaluators occurred when considering the presence or absence of dysplasia; and in addition, it has been observed that less experienced oral pathologists tend to have more severe diagnoses than those with a longer career.
OOOO January 2020 Conclusions: The lack of interobserver agreement was evident, highlighting the need for further studies, due to the great importance and relevance of OEDs.
EOSINOPHILIC ULCER OF THE ORAL MUCOSA: A CLINICOPATHOLOGIC STUDY OF 30 CASES. MARIA FERNANDA CALAZANS FERNANDES, BEATRIZ CORDEIRO, ELLEN BRILHANTE DE ALBUQUERQUE CORTEZZI, ROMAN CARLOS, BRUNO AUGUSTO BENEVENUTO DE ANDRADE, MICHELLE AGOSTINI and, MARIO JOSE e ROMANACH Objective: The aim of this study was to describe the clinicopathologic features of 30 new cases of oral eosinophilic ulcers. Study Design: Clinical data concerning sex, age, affected site, and clinical presentation of 30 patients from Latin America were collected from the files of 2 Oral Pathology Laboratories in a period between 1982 and 2018. Results: The mean age of the patients was 53.3 years, with slight predilection for female patients (1.2:1). Medical history of one-third of patients included psychiatric disorders, alcohol drinking, diabetes mellitus, and human immunodeficiency virus (HIV) infection. The tongue was the most common affected site (76.7%) by lesions with mean size of 1.8 cm and mean evolution of 5.4 months. Raised edges, pain and clinical suspicion of squamous cell carcinoma (SCC) were reported in almost half of cases. Microscopically, all cases revealed a connective tissue covered by fibrinopurulent membrane, containing many capillaries with prominent endothelial cells and reactive inflammatory infiltrate. Variable number of eosinophils was observed, mainly located in the lamina propria, but also deeply between muscle fibers. Conclusions: Eosinophilic ulcer is likely a reactive painful lesion that mainly affects the tongue of adults, which may clinically mimic oral SCC.
PREVALENCE OF ORAL LESIONS IN INDIVIDUALS UP TO 19 YEARS OF AGE: ANALYSIS OF BIOPSIED CASES FROM A SINGLE e VICTOR SILVA BETT, GILBERTO INSTITUTION. JOAO ^ DE SOUZA MELO, ELIS ANGELA BATISTELLA and, ELENA RIET CORREA RIVERO Objective: To determine and compare the prevalence of oral lesion in patients aging from 0 to 9 years and 10 to 19 years. Study Design: Records of biopsies from a single-institution laboratory of oral pathology were screened and data regarding sex, age, and diagnosis were collected. Pearson chi-squared test was applied to determine significant differences on prevalence of lesions between the 2 groups. Results: At total, 296 lesions were assessed; 84 cases from individuals younger than 9 years old and 212 from individuals from 10 to 19 years of age. Overall, mucocele and pyogenic granuloma were the most common lesions diagnosed. A significantly higher prevalence of intraosseous lesions, odontogenic cysts, reactive lesions, and salivary gland lesions were found in individuals aging from 10 to 19 years compared to the younger group (P .05). Conclusions: Mucocele and pyogenic granuloma were the most frequently observed lesions in this study. Individuals aging from 10 to 19 years of age presented significantly higher prevalence
OOOO Volume 129, Number 1 of intraosseous lesions, odontogenic cysts, reactive lesions, and salivary gland lesions compared to younger individuals.
PREVALENCE OF HUMAN PAPILLOMAVIRUS IN LARYNGEAL CANCER SPECIMENS. CRISLYNE MENDES DA VERA CRUZ, LUIZA e DE JESUS MADALENA MENDES DA VERA CRUZ, JOAO e VIANA PINHEIRO, JOSE ISMAEL VIANA DE ARAGAO, HELDER HENRIQUE COSTA PINHEIRO, HELLEN THAIS e ARAUJO FUZII and, MARIZELI VIANA DE ARAGAO Objective: The proposition of this study was to verify prevalence of human papillomavirus (HPV) in larynx neoplastic lesions. Study Design: We analyzed 29 paraffin-embedded specimens with confirmed diagnosis of laryngeal cancer in patients aged 33 to 92 years, of both sexes. Polymerase chain reaction (PCR) was performed for viral detection, followed by 2% agarose gel electrophoresis in TBE. This study was approved by research ethics committee. Results: The results showed the presence of HPV in 72.4% of the sample (21 cases). Conclusions: HPV high prevalence in larynx neoplastic lesions found in the study suggests that HPV is an important etiologic factor in the development of neoplastic lesions. Considering the high mortality due to this cancer, the identification of the virus can contribute to early diagnosis and immediate treatment, since the cancer of the larynx when diagnosed at an early stage, has an excellent prognosis with an 80% to 100% healing power, with decrease of the sequels and a better quality of life for the affected individuals.
PREVALENCE OF ORAL SQUAMOUS CELL CARCINOMA AND ORAL POTENTIALLY MALIGNANT LESIONS DIAGNOSED IN SANTA CATARINA STATE. ACIR JOSE DIRSCHNABEL, JANAINA PITT, FRANCIELLE KAFER, CAMILA PEZZINI, LEA MARIA FRANCESCHI DALLANORA, BRUNA ELIZA DE DEA and, GRASIELI DE OLIVEIRA RAMOS Objectives: The aim of this study was to evaluate the prevalence of potentially malignant and malignant lesions diagnosed in 1 school of dentistry localized in western Santa Catarina State. Study Design: The medical records of patients with potentially malignant lesions and squamous cell carcinoma (SCC) were analyzed. We evaluated the age, sex, occupation, tumor staging, and relation with habits (smoking, and alcohol) during the period from 2012 to 2016. Results: The prevalence ratio between male and female was 3.7: 1.0, with a mean age of 61.96 years, varying between 35 and 85 years. The most common anatomic site was tongue (32.4%) and floor of the moouth (24.3%). Regarding habits, it was reported that most of the patients were smokers (84.6%) and frequently used alcoholic beverages (66.2%). At the time of diagnosis, according to the staging of the tumor, 37.7% of the cases were in the initial stage (T1 and T2) and 62.3% were diagnosed in a late stage (T3 and T4). Conclusions: The results reinforce the need to develop programs and preventive actions for oral cancer in western Santa Catarina, since the cases of SCC were diagnosed in advanced stage, affecting their quality of life, and disease-free survival time.
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DEMOGRAPHIC CHARACTERISTICS OF PATIENTS WITH ACTINIC CHEILITIS IN LABIAL MUCOSA DIAGNOSED IN 2 ONCOLOGIC CARE CENTERS. ACIR JOSE DIRSCHNABEL, VANESSA EINSFELD, CATIANE MOTERLE, ANA CAROLINA DA SILVA, BRUNA ELIZA DE DEA, GRASIELI DE OLIVEIRA RAMOS and, LAURINDO MOACIR SASSI Objective: The aim of this study was to characterize the demographic profile and evaluate the clinical stage of the cases of actinic cheilitis diagnosed in 2 oncologic care centers localized in Curitiba/PR and Joa¸c aba/SC. Study Design: The medical records of patients with potentially malignant lesions and squamous cell carcinoma (SCC) were analyzed. We evaluated the age, sex, occupation, tumor staging, and relation with habits (smoking, and alcohol) during the period from 2012 to 2016. Results: Of the 160 medicals records evaluated, 70.62% were male, mean age 60 years, presenting high sun exposure rate (65%), and 40% of patients reported high-risk occupational occupation. The smoking habit was reported by 44.38% of patients and the alcohol consumption by 21.87%. In 12.5% of actinic cheilitis cases we observed the evolution to squamous cell carcinoma. Conclusions: The use of sunscreen was effective in the prevention of squamous cell carcinoma in lip, and the absent of follow-up care in patients with actinic cheilitis can influence the rates of malignization to squamous cell carcinoma, because the patients does not change the habits and the dentist cannot identify earlier the malignization of actinic cheilitis.
PERIODONTAL THERAPY AFFECTS ORAL COLONIZATION OF CANDIDA SPP AND HIV INFECTION STATUS IN PATIENTS WITH PERIODONTITIS. ATILA VINICIUS VITOR NOBRE, TABATA LARISSA DOS SANTOS POLVORA, CAMILA TIRAPELLI, MARIO TABAJR, MARIA DA e CONCEIC ¸ AO PEREIRA SARAIVA, ALAN GRUPIONI LOURENCO ¸ and, ANA CAROLINA FRAGOSO MOTTA Objectives: This study investigated the effect of nonsurgical periodontal therapy (NSPT) on the clinical and immunologic profile and oral colonization of Candida spp in patients infected with human immunodeficiency virus (HIV) and chronic periodontitis (CP). Study Design: HIV-infected (test group) and non HIVinfected (control group) adults patients with CP were selected. Gingival index (GI), probing depth (PD), clinical attachment level (CAL), number of teeth, CD4+ T lymphocytes and viral load, salivary interleukin (IL)-6, IL-8, and tumor necrosis factoralpha (TNF-a), and Candida spp count were analyzed at baseline, 30, and 90 days after NSPT. Results: Twenty-two patients infected with HIV and 20 patients not infected with HIV were evaluated. Candida spp count and salivary IL-6, IL-8, and TNF-a levels were higher in the test group than in the control group. Both groups presented decrease in Candida spp oral count, GI, PD, IL-6, and IL-8 as well as gain of CAL after NSPT at 30 and 90 days. In addition, patients from test group showed an increase of CD4+ T lymphocytes and a decrease of viral load. Conclusions: NSPT had a beneficial effect on the clinical parameters of CP and on the Candida spp oral count and
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IMMUNOHISTOCHEMICAL COMPARATIVE STUDY OF PERIPHERAL, PERI-IMPLANT, AND CENTRAL GIANT CELL LESIONS. FABIO RAMOA PIRES, THAYNA MELO DE LIMA MORAIS, CIRO DANTAS SOARES, JOSE MANUEL AGUIRRE URIZAR, JAVIER ALBERDI NAVARRO and, OSLEI PAES DE ALMEIDA Central giant cell lesions (CGCL) and peripheral giant cell lesions (PGCL) are reactive lesions of unknown etiopathogenesis and similar histologic features. PGCL affects the gingiva and alveolar mucosa, but some rare cases can be associated with dental implants. Objective: In the present study, we compared the expression of smooth muscle actin, Bcl-2, GLUT-1, CD68, OPG, RANK, Ki67, and CD34 in a series of CGCL and conventional and periimplant PGCL. Study Design: Clinical data were retrieved from the laboratory records and histologic analysis was performed in hematoxylin and eosin (H&E)-stained sections. Immunohistochemical reactions for detecting the above mentioned antibodies were performed and digitally scored. Results: The digital scores for CD68 and Bcl-2 were higher in conventional PGCL and CGCL than in peri-implant PGCL (P = .033 for CD68 and P <.0001 for Bcl-2). Microvessel density was higher in the conventional PGCL than in CGCL and periimplant PGCL (P = .002). Conclusions: The current results demonstrated some differences in microvessel density, proliferative activity, and expression of CD68 and Bcl-2 among conventional PGCL, periimplant PGCL and CGCL. Further studies are encouraged to better understanding of these early findings.
COMPARATIVE STUDY AMONG HISTOLOGIC GRADUATION SYSTEMS OF ORAL EPITHELIAL DYSPLASIAS. JULIANA COSTA DE OLIVEIRA, GABRIELA MEDEIROS DE CERQUEIRA, e JOAO FIGUEIRA SCARINI, PRISCILLA RODRIGUES ^ CAMARA, REBECA DE SOUZA AZEVEDO, DECIO DOS SANTOS PINTO JUNIOR and, RENATA TUCCI Objective: The objective of this study was to evaluate the histologic grading systems for oral epithelial dysplasia (ODE). Study Design: One hundred slides of ODE containing cases selected from 2 distinct oral pathology services were examined by 4 evaluators, who classified each case according to the architectural and cytologic criteria and were diagnosed according to the World Health Organization (WHO) system and binary. In addition to these 2 systems, a third form of analysis was considered, taking into account the presence or absence of ODE. Results: The results were statistically analyzed in a descriptive way and by the correlation methods (Kendall tau-B and Phi test) and concordance (kappa). The WHO graduation system presented lower agreement among the evaluators; the best agreement among the evaluators occurred when considering the presence or absence of dysplasia; and in addition, it has been observed that less experienced oral pathologists tend to have more severe diagnoses than those with a longer career.
OOOO January 2020 Conclusions: The lack of interobserver agreement was evident, highlighting the need for further studies, due to the great importance and relevance of OEDs.
EOSINOPHILIC ULCER OF THE ORAL MUCOSA: A CLINICOPATHOLOGIC STUDY OF 30 CASES. MARIA FERNANDA CALAZANS FERNANDES, BEATRIZ CORDEIRO, ELLEN BRILHANTE DE ALBUQUERQUE CORTEZZI, ROMAN CARLOS, BRUNO AUGUSTO BENEVENUTO DE ANDRADE, MICHELLE AGOSTINI and, MARIO JOSE e ROMANACH Objective: The aim of this study was to describe the clinicopathologic features of 30 new cases of oral eosinophilic ulcers. Study Design: Clinical data concerning sex, age, affected site, and clinical presentation of 30 patients from Latin America were collected from the files of 2 Oral Pathology Laboratories in a period between 1982 and 2018. Results: The mean age of the patients was 53.3 years, with slight predilection for female patients (1.2:1). Medical history of one-third of patients included psychiatric disorders, alcohol drinking, diabetes mellitus, and human immunodeficiency virus (HIV) infection. The tongue was the most common affected site (76.7%) by lesions with mean size of 1.8 cm and mean evolution of 5.4 months. Raised edges, pain and clinical suspicion of squamous cell carcinoma (SCC) were reported in almost half of cases. Microscopically, all cases revealed a connective tissue covered by fibrinopurulent membrane, containing many capillaries with prominent endothelial cells and reactive inflammatory infiltrate. Variable number of eosinophils was observed, mainly located in the lamina propria, but also deeply between muscle fibers. Conclusions: Eosinophilic ulcer is likely a reactive painful lesion that mainly affects the tongue of adults, which may clinically mimic oral SCC.
PREVALENCE OF ORAL LESIONS IN INDIVIDUALS UP TO 19 YEARS OF AGE: ANALYSIS OF BIOPSIED CASES FROM A SINGLE e VICTOR SILVA BETT, GILBERTO INSTITUTION. JOAO ^ DE SOUZA MELO, ELIS ANGELA BATISTELLA and, ELENA RIET CORREA RIVERO Objective: To determine and compare the prevalence of oral lesion in patients aging from 0 to 9 years and 10 to 19 years. Study Design: Records of biopsies from a single-institution laboratory of oral pathology were screened and data regarding sex, age, and diagnosis were collected. Pearson chi-squared test was applied to determine significant differences on prevalence of lesions between the 2 groups. Results: At total, 296 lesions were assessed; 84 cases from individuals younger than 9 years old and 212 from individuals from 10 to 19 years of age. Overall, mucocele and pyogenic granuloma were the most common lesions diagnosed. A significantly higher prevalence of intraosseous lesions, odontogenic cysts, reactive lesions, and salivary gland lesions were found in individuals aging from 10 to 19 years compared to the younger group (P .05). Conclusions: Mucocele and pyogenic granuloma were the most frequently observed lesions in this study. Individuals aging from 10 to 19 years of age presented significantly higher prevalence
OOOO Volume 129, Number 1 of intraosseous lesions, odontogenic cysts, reactive lesions, and salivary gland lesions compared to younger individuals.
PREVALENCE OF HUMAN PAPILLOMAVIRUS IN LARYNGEAL CANCER SPECIMENS. CRISLYNE MENDES DA VERA CRUZ, LUIZA e DE JESUS MADALENA MENDES DA VERA CRUZ, JOAO e VIANA PINHEIRO, JOSE ISMAEL VIANA DE ARAGAO, HELDER HENRIQUE COSTA PINHEIRO, HELLEN THAIS e ARAUJO FUZII and, MARIZELI VIANA DE ARAGAO Objective: The proposition of this study was to verify prevalence of human papillomavirus (HPV) in larynx neoplastic lesions. Study Design: We analyzed 29 paraffin-embedded specimens with confirmed diagnosis of laryngeal cancer in patients aged 33 to 92 years, of both sexes. Polymerase chain reaction (PCR) was performed for viral detection, followed by 2% agarose gel electrophoresis in TBE. This study was approved by research ethics committee. Results: The results showed the presence of HPV in 72.4% of the sample (21 cases). Conclusions: HPV high prevalence in larynx neoplastic lesions found in the study suggests that HPV is an important etiologic factor in the development of neoplastic lesions. Considering the high mortality due to this cancer, the identification of the virus can contribute to early diagnosis and immediate treatment, since the cancer of the larynx when diagnosed at an early stage, has an excellent prognosis with an 80% to 100% healing power, with decrease of the sequels and a better quality of life for the affected individuals.
PREVALENCE OF ORAL SQUAMOUS CELL CARCINOMA AND ORAL POTENTIALLY MALIGNANT LESIONS DIAGNOSED IN SANTA CATARINA STATE. ACIR JOSE DIRSCHNABEL, JANAINA PITT, FRANCIELLE KAFER, CAMILA PEZZINI, LEA MARIA FRANCESCHI DALLANORA, BRUNA ELIZA DE DEA and, GRASIELI DE OLIVEIRA RAMOS Objectives: The aim of this study was to evaluate the prevalence of potentially malignant and malignant lesions diagnosed in 1 school of dentistry localized in western Santa Catarina State. Study Design: The medical records of patients with potentially malignant lesions and squamous cell carcinoma (SCC) were analyzed. We evaluated the age, sex, occupation, tumor staging, and relation with habits (smoking, and alcohol) during the period from 2012 to 2016. Results: The prevalence ratio between male and female was 3.7: 1.0, with a mean age of 61.96 years, varying between 35 and 85 years. The most common anatomic site was tongue (32.4%) and floor of the moouth (24.3%). Regarding habits, it was reported that most of the patients were smokers (84.6%) and frequently used alcoholic beverages (66.2%). At the time of diagnosis, according to the staging of the tumor, 37.7% of the cases were in the initial stage (T1 and T2) and 62.3% were diagnosed in a late stage (T3 and T4). Conclusions: The results reinforce the need to develop programs and preventive actions for oral cancer in western Santa Catarina, since the cases of SCC were diagnosed in advanced stage, affecting their quality of life, and disease-free survival time.
ABSTRACTS
e179
DEMOGRAPHIC CHARACTERISTICS OF PATIENTS WITH ACTINIC CHEILITIS IN LABIAL MUCOSA DIAGNOSED IN 2 ONCOLOGIC CARE CENTERS. ACIR JOSE DIRSCHNABEL, VANESSA EINSFELD, CATIANE MOTERLE, ANA CAROLINA DA SILVA, BRUNA ELIZA DE DEA, GRASIELI DE OLIVEIRA RAMOS and, LAURINDO MOACIR SASSI Objective: The aim of this study was to characterize the demographic profile and evaluate the clinical stage of the cases of actinic cheilitis diagnosed in 2 oncologic care centers localized in Curitiba/PR and Joa¸c aba/SC. Study Design: The medical records of patients with potentially malignant lesions and squamous cell carcinoma (SCC) were analyzed. We evaluated the age, sex, occupation, tumor staging, and relation with habits (smoking, and alcohol) during the period from 2012 to 2016. Results: Of the 160 medicals records evaluated, 70.62% were male, mean age 60 years, presenting high sun exposure rate (65%), and 40% of patients reported high-risk occupational occupation. The smoking habit was reported by 44.38% of patients and the alcohol consumption by 21.87%. In 12.5% of actinic cheilitis cases we observed the evolution to squamous cell carcinoma. Conclusions: The use of sunscreen was effective in the prevention of squamous cell carcinoma in lip, and the absent of follow-up care in patients with actinic cheilitis can influence the rates of malignization to squamous cell carcinoma, because the patients does not change the habits and the dentist cannot identify earlier the malignization of actinic cheilitis.
PERIODONTAL THERAPY AFFECTS ORAL COLONIZATION OF CANDIDA SPP AND HIV INFECTION STATUS IN PATIENTS WITH PERIODONTITIS. ATILA VINICIUS VITOR NOBRE, TABATA LARISSA DOS SANTOS POLVORA, CAMILA TIRAPELLI, MARIO TABAJR, MARIA DA e CONCEIC ¸ AO PEREIRA SARAIVA, ALAN GRUPIONI LOURENCO ¸ and, ANA CAROLINA FRAGOSO MOTTA Objectives: This study investigated the effect of nonsurgical periodontal therapy (NSPT) on the clinical and immunologic profile and oral colonization of Candida spp in patients infected with human immunodeficiency virus (HIV) and chronic periodontitis (CP). Study Design: HIV-infected (test group) and non HIVinfected (control group) adults patients with CP were selected. Gingival index (GI), probing depth (PD), clinical attachment level (CAL), number of teeth, CD4+ T lymphocytes and viral load, salivary interleukin (IL)-6, IL-8, and tumor necrosis factoralpha (TNF-a), and Candida spp count were analyzed at baseline, 30, and 90 days after NSPT. Results: Twenty-two patients infected with HIV and 20 patients not infected with HIV were evaluated. Candida spp count and salivary IL-6, IL-8, and TNF-a levels were higher in the test group than in the control group. Both groups presented decrease in Candida spp oral count, GI, PD, IL-6, and IL-8 as well as gain of CAL after NSPT at 30 and 90 days. In addition, patients from test group showed an increase of CD4+ T lymphocytes and a decrease of viral load. Conclusions: NSPT had a beneficial effect on the clinical parameters of CP and on the Candida spp oral count and