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A RETROSPECTIVE STUDY OF BIOPSIED ORAL LESIONS IN A POPULATION FROM A BRAZILIAN FAVELA
OOOO Volume 129, Number 1 Study Design: Lymphocytes exhibiting nuclear immunopositivity for FoxP3 were quantified at the tumor invasive front of 48 OTSCCs (24 diagnosed in young patients and 24 diagnosed in older patients). The results were analyzed statistically using the nonparametric Mann-Whitney test. Results: The number of FoxP3+ lymphocytes in OTSCCs tended to be higher in older patients (P = .055). Analysis of OTSCCs in males and in early clinical stages disclosed a higher number of Treg cells in older patients compared with young patients (P < .05). In older patients, the number of Treg cells tended to be higher in smaller tumors (P = .079). Tumors with intense inflammatory infiltrate exhibited a larger number of Treg cells, both in young (P = .099) and older patients (P = .005). Conclusions: The results suggest a greater participation of Treg cells in immunoinflammatory responses in the microenvironment of OTSCCs in older patients, particularly in males and in early stages.
EPITHELIAL-MESENCHYMAL TRANSITION AND CELL PROLIFERATION IN ORAL AND OROPHARYNGEAL SQUAMOUS CELL CAR CINOMAS. JORGE ESQUICHE LEON, MAYKON KENNEDY SCHULZ OLIVEIRA, SUZIENE CAROLINE DA SILVA, ANDRESSA DUARTE, HEITOR ALBERGONI DA SILVEIRA, ALFREDO RIBEIRO-SILVA and, LUCIANA YAMAMOTO ALMEIDA Objective: Oropharyngeal squamous cell carcinomas (OPSCCs), especially those expressing human papilloma virus (HPV), are associated with a better prognosis than HPV-negative OPSCC and oral squamous cell carcinoma (OSCC). Among them, OPSCC often presents nonkeratinizing and hybrid than keratinizing histologic subtypes, as well as higher proliferation index. Thus, this study aims to analyze the relationship of epithelial-mesenchymal transition (EMT) and cell proliferation markers in OPSCC and OSCC. Study Design: Analysis of EMT (vimentin, CD10) and cell proliferation (cyclin D1 and Ki-67 labeling index [LI]) immunomarkers were performed on tissue microarrays containing 58 OSCC and 17 OPSCC formalin-fixed paraffin-embedded samples and correlations with the histopathological grading were made. Results: Our results showed positivity for vimentin (4 OSCC; 1 OPSCC) and CD10 (8 OSCC; 2 OPSCC). Different from Ki-67 (OSCC, LI 45.5%; OPSCC, LI 41.2%), in which all cases were positive, cyclin D1 was positive only in 53 OSCC (LI 35.5%) and 14 OPSCC (LI 26.6%) cases. No correlations between EMT markers and OSCC/OPSCC histopathologic grading, and similar OSCC/OPSCC cell proliferation, were observed. Conclusions: Our results suggest similar expression profiles of EMT and cell proliferation markers in OSCC and OPSCC. Other studies are necessary to efficiently explain why OPSCC have a significantly more favorable prognosis than OSCC. FAPESP grant: 2016/11419-0.
A RETROSPECTIVE STUDY OF BIOPSIED ORAL LESIONS IN A POPULATION FROM A BRAZILIAN FAVELA. MARIA FERNANDA CALAZANS FERNANDES, FABIOLLA SOARES e ^ A ABRAHAO, BARBOSA, ALINE CORRE BRUNO AUGUSTO BENEVENUTO DE ANDRADE, MARCIA e GRILLO CABRAL, MARIO JOSE ROMANACH and, MICHELLE AGOSTINI
ABSTRACTS
e165
Oral lesions may vary significantly from 1 region to another, considering the socioeconomic, environmental, cultural, and infrastructural conditions of the patients. Epidemiologic data are essential to evaluate the distribution of oral diseases and to develop appropriate prevention methods. Objective: The aim of the present study is to report a survey of oral lesions biopsied in patients from one of the largest favela (slums) from Brazil. Study Design: Clinical records and microscopic diagnosis of oral lesions of these patients were retrieved from the files of an oral pathology laboratory from Brazil between 2016 and 2017. Results: A total of 84 biopsies were performed. Most patients (66%) were women with average age of 44 years. Twenty-five different diagnoses were signed out, being the most common: fibrous hyperplasia (38.5%), mucocele (12%), and squamous cell carcinoma (SCC) (9.5%). Conclusion: There was a significant number of SCC, the most common oral cancer, which was commonly detected in smokers and drinkers of this population. Preventive actions against tobacco use and alcohol consumption habits are required in order to decrease the incidence of oral cancer in this favela population. These actions should include proposals that can help to improve the social reality of this community.
EVALUATION OF THE EFFECTIVENESS OF THE MOUTH ADEQUATE PROTOCOL IN THE PREVENTION OF ORAL MUCOSITIS IN ONCOLOGIC PATIENT. SARAH RACHEL CAVALCANTE e BEZERRA MELO, TEREZA PAULA DE LIMA GUSMAO, ADRIANA MACHADO ZARZAR, ANDREZA BARKOKEBAS SANTOS DE FARIA, LUIZ ALCINO e and, ALESSANDRA DE GUEIROS, JAIR CARNEIRO LEAO ALBUQUERQUE TAVARES CARVALHO Objective: To evaluate the efficacy of the oral medium adequacy protocol in patients with cancer subjected to antineoplastic treatment. Study Design: A cohort was performed. The sample consisted of 100 adult patients, 72 patients undergoing head and neck radiotherapy, 3 receiving bisphosphonate, and 69 receiving 5-fluoruracil, cyclophosphamide, taxol, and metrotrexate. The protocol established for the adaptation of the oral medium included: dental scaling and polishing, tooth extraction, and composite resin restorations. In addition to mouthwashes with chlorhexidine 0.12% without alcohol (12 in 12 hours); dexpanthenol ointment for lip hydration (2/2 per hr), mouthwashes with iced chamomile tea (2 in 2 hours), carboxymethylcellulose 2% artificial saliva (6 every 6 hours) for patients with xerostomia, and 20-second and 2-J low intensity laser therapy, toothed patients were also prescribed 0.05% sodium fluoride for mouthwashes every 24 hours. Results: Of the treated patients 2 developed grade I mucositis, 23 developed grade II mucositis, and 69 did not present mucositis. We did not observe the need to enter parenteral nutrition nor to interrupt the antineoplastic treatment in any of the patients. Conclusions: The oral medium adequacy protocol was effective in preventing grade III and IV mucositis and discontinuation of antineoplastic treatment.
CLINICOPATHOLOGIC STUDY AND MOLECULAR ANALYSIS OF HUMAN
EPITHELIAL-MESENCHYMAL TRANSITION AND CELL PROLIFERATION IN ORAL AND OROPHARYNGEAL SQUAMOUS CELL CAR CINOMAS. JORGE ESQUICHE LEON, MAYKON KENNEDY SCHULZ OLIVEIRA, SUZIENE CAROLINE DA SILVA, ANDRESSA DUARTE, HEITOR ALBERGONI DA SILVEIRA, ALFREDO RIBEIRO-SILVA and, LUCIANA YAMAMOTO ALMEIDA Objective: Oropharyngeal squamous cell carcinomas (OPSCCs), especially those expressing human papilloma virus (HPV), are associated with a better prognosis than HPV-negative OPSCC and oral squamous cell carcinoma (OSCC). Among them, OPSCC often presents nonkeratinizing and hybrid than keratinizing histologic subtypes, as well as higher proliferation index. Thus, this study aims to analyze the relationship of epithelial-mesenchymal transition (EMT) and cell proliferation markers in OPSCC and OSCC. Study Design: Analysis of EMT (vimentin, CD10) and cell proliferation (cyclin D1 and Ki-67 labeling index [LI]) immunomarkers were performed on tissue microarrays containing 58 OSCC and 17 OPSCC formalin-fixed paraffin-embedded samples and correlations with the histopathological grading were made. Results: Our results showed positivity for vimentin (4 OSCC; 1 OPSCC) and CD10 (8 OSCC; 2 OPSCC). Different from Ki-67 (OSCC, LI 45.5%; OPSCC, LI 41.2%), in which all cases were positive, cyclin D1 was positive only in 53 OSCC (LI 35.5%) and 14 OPSCC (LI 26.6%) cases. No correlations between EMT markers and OSCC/OPSCC histopathologic grading, and similar OSCC/OPSCC cell proliferation, were observed. Conclusions: Our results suggest similar expression profiles of EMT and cell proliferation markers in OSCC and OPSCC. Other studies are necessary to efficiently explain why OPSCC have a significantly more favorable prognosis than OSCC. FAPESP grant: 2016/11419-0.
A RETROSPECTIVE STUDY OF BIOPSIED ORAL LESIONS IN A POPULATION FROM A BRAZILIAN FAVELA. MARIA FERNANDA CALAZANS FERNANDES, FABIOLLA SOARES e ^ A ABRAHAO, BARBOSA, ALINE CORRE BRUNO AUGUSTO BENEVENUTO DE ANDRADE, MARCIA e GRILLO CABRAL, MARIO JOSE ROMANACH and, MICHELLE AGOSTINI
ABSTRACTS
e165
Oral lesions may vary significantly from 1 region to another, considering the socioeconomic, environmental, cultural, and infrastructural conditions of the patients. Epidemiologic data are essential to evaluate the distribution of oral diseases and to develop appropriate prevention methods. Objective: The aim of the present study is to report a survey of oral lesions biopsied in patients from one of the largest favela (slums) from Brazil. Study Design: Clinical records and microscopic diagnosis of oral lesions of these patients were retrieved from the files of an oral pathology laboratory from Brazil between 2016 and 2017. Results: A total of 84 biopsies were performed. Most patients (66%) were women with average age of 44 years. Twenty-five different diagnoses were signed out, being the most common: fibrous hyperplasia (38.5%), mucocele (12%), and squamous cell carcinoma (SCC) (9.5%). Conclusion: There was a significant number of SCC, the most common oral cancer, which was commonly detected in smokers and drinkers of this population. Preventive actions against tobacco use and alcohol consumption habits are required in order to decrease the incidence of oral cancer in this favela population. These actions should include proposals that can help to improve the social reality of this community.
EVALUATION OF THE EFFECTIVENESS OF THE MOUTH ADEQUATE PROTOCOL IN THE PREVENTION OF ORAL MUCOSITIS IN ONCOLOGIC PATIENT. SARAH RACHEL CAVALCANTE e BEZERRA MELO, TEREZA PAULA DE LIMA GUSMAO, ADRIANA MACHADO ZARZAR, ANDREZA BARKOKEBAS SANTOS DE FARIA, LUIZ ALCINO e and, ALESSANDRA DE GUEIROS, JAIR CARNEIRO LEAO ALBUQUERQUE TAVARES CARVALHO Objective: To evaluate the efficacy of the oral medium adequacy protocol in patients with cancer subjected to antineoplastic treatment. Study Design: A cohort was performed. The sample consisted of 100 adult patients, 72 patients undergoing head and neck radiotherapy, 3 receiving bisphosphonate, and 69 receiving 5-fluoruracil, cyclophosphamide, taxol, and metrotrexate. The protocol established for the adaptation of the oral medium included: dental scaling and polishing, tooth extraction, and composite resin restorations. In addition to mouthwashes with chlorhexidine 0.12% without alcohol (12 in 12 hours); dexpanthenol ointment for lip hydration (2/2 per hr), mouthwashes with iced chamomile tea (2 in 2 hours), carboxymethylcellulose 2% artificial saliva (6 every 6 hours) for patients with xerostomia, and 20-second and 2-J low intensity laser therapy, toothed patients were also prescribed 0.05% sodium fluoride for mouthwashes every 24 hours. Results: Of the treated patients 2 developed grade I mucositis, 23 developed grade II mucositis, and 69 did not present mucositis. We did not observe the need to enter parenteral nutrition nor to interrupt the antineoplastic treatment in any of the patients. Conclusions: The oral medium adequacy protocol was effective in preventing grade III and IV mucositis and discontinuation of antineoplastic treatment.
CLINICOPATHOLOGIC STUDY AND MOLECULAR ANALYSIS OF HUMAN