- Email: [email protected]
PS319 Microrna-24 Attenuates Neointimal Hyperplasia in the Diabetic Rat Carotid Artery Injury Model By Inhibiting Wnt4 Signaling Pathway
POSTER ABSTRACTS
knowledge and attitude scores (r¼0.224, p<0.001), as well as knowledge and practice scores (r¼0.114, p<0.001). There was no correlation between attitude and practice scores. Significant multivariate predictors of higher knowledge scores included female sex, higher level of education, higher attitude and practice scores and prior coronary artery disease. Significant multivariate predictors of higher attitude scores included higher level of education, higher knowledge scores and non-Indian ethnicity. Significant multivariate predictors of higher practice scores included male sex, Indian ethnicity, older age, higher knowledge score and hypertension. Males had significantly lower knowledge scores (85.88.0% vs 88.08.2%, p<0.001), lower attitude scores (91.49.4% vs 93.28.3%, p¼0.005) and higher practice scores (58.418.7% vs 55.119.3%, p¼0.013) than females. Conclusion: In our Asian cohort, knowledge of cardiovascular health plays a significant role in influencing attitudes and practices. There exist significant gender differences in KAP. Adopting gender-specific strategies for future public health campaigns could address the above gender differences. Disclosure of Interest: None Declared
underlying pathology (atherosclerosis, restenosis, radiation-induced arteriopathy, vasculitis), lesion location, and stenosis grade. Calcifications, long lesions (> 20 mm), and long balloon-expandable stents (> 20 mm) were more common in the fractured than in the non-fractured group (86.0% versus 43.0%, P < 0.001; 51.2% versus 20.3%, P < 0.001; 62.8% versus 40.5%, P ¼ 0.036, respectively). The restenosis and reintervention rates were significantly higher in patients with type III-V fractures compared to those with type I-II fractures (60.0% versus 3.6%, P < 0.001; 46.7% versus 3.6%, P < 0.001, respectively). Conclusion: Fractures frequently occur. Lesion and stent characteristics have an influence on fracture rate. Complex fractures increase the risk of restenosis. Disclosure of Interest: None Declared
PS319 Microrna-24 Attenuates Neointimal Hyperplasia in the Diabetic Rat Carotid Artery Injury Model By Inhibiting Wnt4 Signaling Pathway
PS314
J. Yang*1, Z.-X. Fan2, J. Yang1, J.-W. Ding2, C.-J. Yang2, P. Zeng2 Department of Cardiology, the First College of Clinical Medical Sciences, China Three Gorges University, Yichang 443000, Hubei Province, China, 2The First College of Clinical Medical Sciences, China Three Gorges University, Yichang, China 1
Rosuvastatin Dose-Dependently Causes Insulin Resistance and Increases Ambient Glycemia in Hypercholesterolemic Patients K. Koh*1 1 Gachon University Gil Medical Center, Incheon, Republic Of Korea Introduction: Genetic analysis from patients participated in the randomised trials reported that the increased risk of type 2 diabetes noted with statins is at least partially explained by HMG-coenzyme A reductase inhibition. Objectives: We investigated whether rosuvastatin dose-dependently decreases insulin sensitivity and increases ambient glycemia in hypercholesterolemic patients. Methods: This was a randomized, single-blind, placebo-controlled, parallel study. Age, sex, and body mass index were matched among groups. Forty-eight patients were given placebo, and 47, 48, and 47 patients given rosuvastatin 5,10, and 20 mg, respectively daily during a 2 month treatment period. Results: Placebo therapy did not significantly change insulin, adiponectin, glycated hemoglobin, and insulin sensitivity relative to baseline measurements. Rosuvastatin 5,10, and 20 mg dose-dependently and significantly increased insulin (median % changes; 16, 20, and 20%, respectively) and glycated hemoglobin levels (mean % changes; 2, 2, and 3%, respectively), and decreased adiponectin levels (mean % changes; 3, 9, and 14%, respectively) and insulin sensitivity (mean % changes; 2, 3, and 4%, respectively) after 2 months therapy when compared with either baseline (all P<0.05 by paired t-test). These effects with rosuvastatin 5,10, and 20 mg were significant when compared with placebo (P¼0.006 for insulin, P¼0.012 for glycated hemoglobin, P¼0.007 for adiponectin, and P¼0.002 for insulin sensitivity by ANOVA). Conclusion: Despite beneficial reductions in LDL cholesterol and apolipoprotein B, rosuvastatin treatment dose-dependently and significantly resulted in decreasing insulin sensitivity and increasing ambient glycemia by reducing adiponectin levels and increasing insulin levels in hypercholesterolemic patients. Disclosure of Interest: None Declared
Introduction: Diabetes mellitus (DM) is a worldwide health problem, in both the developed and developing countries. Long-term stimulus of hyperglycemia greatly increases the incidence of vascular restenosis (RS) after angioplasty (such as percutaneous coronary intervention, PCI). MicroRNA-24(miR-24) is low expression in diabetic rat carotid artery after balloon injury. However, the role of miR-24 in vascular system is unknown.
PS317 Incidence and Risk Factors for Subclavian Artery Stent Fracture E. Dósa*1, A. G. Hüttl1, A. Hüttl1, E. M. Végh1, B. Nemes1, K. Hüttl1 1 Heart and Vascular Center, Semmelweis University, Budapest, Hungary Introduction: Stenting is the preferred, minimally invasive treatment for subclavian artery (SA) steno-occlusive disease. Stent fractures in the SA have not been assessed in large cohorts. Objectives: To determine the prevalence of SA stent fractures, identify predisposing factors, and analyze their impact on restenosis development. Methods: 120 patients (71 women; mean age: 59.4+/-9.0 years) with symptomatic significant SA stenosis who underwent stenting between 2000 and 2012 at our Department have been included in the study. In 2014, patients were asked to return for a fluoroscopic examination of the implanted stents. Stent fractures were defined as type I: single-strut fracture, type II: > 2 strut fractures without deformation, type III: > 2 strut fractures with deformation, type IV: multiple strut fractures with acquired transection but without gap, and type V: multiple strut fractures with acquired transection with gap in the stent body. Color duplex scan has been used to monitor stent patency. In case of continuous variables t or Mann-Whitney U test, while in case of categorical variables chi-square or Fisher exact test were performed. Results: 122 stents have been deployed (balloon-expandable, n ¼ 94; self-expandable, n ¼ 28). The median follow-up time was 81.4 (41.1-113.7) months. 43 (35.2%) stent fractures were detected (type I-II, n ¼ 28; type III-V, n ¼ 15). The difference between the fractured (n ¼ 43) and non-fractured (n ¼ 79) groups was non-significant regarding the atherosclerotic risk factors (age > 70 years, female sex, smoking, hypertension, hyperlipidemia, diabetes mellitus, obesity, chronic kidney disease),
e68
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Methods: Group A ¼ 32 volunteers in the last year of Medical school was compared to Group B ¼ 24 volunteers of human sciences school. Both groups received theoretical and practical training in cardiopulmonary resuscitation and automated external defibrillator (AED) based on the methodology of Basic Life Support courses (BLS) from American Heart Association. At the end of the course, and after 30 and 60 days, all students were evaluated for their ability to perform CPR and the AED, using manikin ResusciAnne Skill Reporter - Laerdal Ò Company. The practical evaluation was recorded on DVD for later evaluation by experienced instructors that did not know the students. A checklist created by BLS practical evaluation for Health care providers was used. Previous trained students and those who did not agree to participate in the project were excluded. Results: In practical evaluation, human sciences students had lower score than medical students at 30 and 60 days (p<0,05). In practical evaluations at 30 to 60 days, human sciences students had lower scores in 60 days than in 30 days. Parameters like compression depth and ventilation rate were equal for both groups. Conclusion: Physiological understanding influence in maintaining the ability to perform CPR without significantly interference with the quality of compression or ventilation. Disclosure of Interest: None Declared PS324 Characterization of Bleeding and Thrombotic Events Dialysis Patients Who are Treated with Antithrombotic Agents C. M. Doria Murcia*1, R. M. Rodríguez Amaya1, C. L. Figueroa Pineda1 1 Universidad Industrial de Santander, Grupo GERMINA, Bucaramanga, Colombia
PS320 Cardiopulmonary Resuscitation Training Using Realtime Quantitative System A.-A. Augustine1, R. Subramaniam1, M. Timam1, A. Bungsu1, S. Y. Liau2, P. N. Arumuganathan*1, Y. W. Lee1, C. H. Yen1, G. Ma1, H. B. Liew1 1 Cardiology, 2Clinical Research Centre, Queen Elizabeth II Hospital, Kota Kinabalu, Malaysia Introduction: Cardiopulmonary resuscitation (CPR) is an important skillset for all healthcare workers (HCW) in the event of a cardiopulmonary arrest. Effective CPR requires training, to achieve and maintain adequate speed and depth of compression in the right position. Mannequin with immediate quantitative assessment can facilitate CPR training course, by providing real time feedback to both trainer and trainees. Objectives: To evaluate the effectiveness of realtime feedback mannequin in CPR training, by comparing pre and post-training. Methods: This was a single centre study (tertiary cardiac centre). Between 10th October 2014 to 4th September 2015, a total of 33 HCW underwent the CPR training sessions. CPR course began with a basic lecture regarding CPR standards, and demonstration of the mannequin. Pre-training assessment was conducted, and result feedback were immediate, followed by a practice on mannequins to improve speed and depth of compressions. After a period of practice, reassessment performed. Data collection was automated in the Laerdal mannequin system. Candidates were staff from the cardiology department. Data was entered into SPSS and analysed descriptively. Comparative analysis of variables pre versus post training was calculated using paired sample T Test. A two-tailed P-value < 0.05 was considered statistically significant. Results: Mean age was 26.3 years old, 66.7% female; majority of subjects were nurses 51.5%; average years of practice in medical field was 2.8 years. No significant differences in compression depth (53.76 vs 52.48), and hand position (93.6% vs 98.0%). However, there is notable improvement in compression rate (45.7% vs 68.3%) and recoil (62.27% vs 73.97%, p value > 0.05). There was no correlation between years of service and total score (r ¼ 0.063). Conclusion: Our study showed there was no significant change of results in pre and post CPR training amongst HCW who underwent training using the real time feedback mannequin system. Future use of quantitative CPR assessment in training of non-HCW, example lay public would be needed to further establish the usefulness and effectiveness of such technology. Disclosure of Interest: None Declared
Introduction: The End-Stage Renal Disease ESRD, is one of the diseases fastest growing worldwide turn is classified as a public health problem due to increased morbidity, mortality and represents high costs to the system of health. Moreover, current evidence has shown that cardiovascular risk scales used in the general population, are not fully extrapolated to the group of patients with ESRD and that in several cases the results of the studies have been inconclusive in determining whether there is a increased risk of bleeding or thromboembolic complications. Objectives: The aim of the study was to determine sociodemographic and clinical aspects of dialysis patients who are treated with antithrombotic agents and determine the frequency of thrombotic events and bleeding from a historical cohort during the period from 2009 through 2013. Methods: An observational study was conducted and a historical cohort, which included patients with ESRD over 18 years, most dialysis at 90 days, treatment modality dialysis, automated peritoneal dialysis and continuous ambulatory peritoneal dialysis, they had prescription studied antithrombotic agent and belonging to a renal clinic Renal Therapy Service (RTS) in ColombiaÒ, in the time period between January 1, 2009 at 31 December 2013. We excluded those with liver disease or other concomitant disorders coagulation. The sample size calculation was probabilistic, through a scheme simple random sampling treatment groups. It was taken to the execution of the study endorsed by the ethics committee of the Industrial University of Santander. Results: A final sample of 965 patients was achieved, mainly male (61%) with a mean age of 61.8 13.5 years. Diabetes (46.1%) was the major cause of renal disease followed by hypertension (24.6%). In addition, 62% of patients had started dialysis as an emergency. Also, 159 patients had a thrombotic or hemorrhagic event, 11.4% had a thrombotic event and 6.5% experienced a bleeding event. Table 1 attached distribution of bleeding and thrombotic events according to the group of drugs received at the start of dialysis treatment in the cohort is appreciated.
PS321 Effect of Physiological Understanding In CPR Skill Retention H. C. Miotto1, I. Z. Miotto1, R. R. Oliveira1, M. D. C. V. Moreira*1 Internal Medicine, Federal University of Minas Gerais, Belo Horizonte, Brazil
1
Introduction: Retention of CPR skills deteriorates very rapidly. We made the hypothesis that lay rescuer loses their ability sooner than health provider. Objectives: Assess whether the level of physiological understanding of cardiopulmonary resuscitation may influence the learning and retention of motor skills.
GHEART Vol 11/2S/2016
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June, 2016
j
POSTER/WCC_2016-POSTERS
Conclusion: In light of the data and still having a higher average retail creatinine and hemoglobin, the group of drugs with less presence of thrombotic and bleeding events was the LMWH, there was also an increased risk of bleeding events tormbóticos present. Disclosure of Interest: None Declared
e69
POSTER ABSTRACTS
Objectives: To explore whether over-expression miR-24 could attenuate neointimal formation in streptozotocin (STZ) induced diabetic rats. Methods: Adenovirus (Ad-miR-24-GFP) was used to delivery gene (miR-24) to injury carotid artery in diabetic rats. The degree of neointimal hyperplasia was evaluated by hematoxylin-eosin (HE) staining. Vascular smooth muscle cells (VSMCs) proliferation in the neointima of injury artery was examined by immunostaining for proliferating cell nuclear antigen (PCNA). The mRNA levels of miR-24 and cell cycle-associated molecules (Cyclin D1, p21) were determined by quantitative real-time PCR (qRT-PCR) analysis. PCNA, Wnt4, Disheveled-1(Dvl-1), b-catenin, Cyclin D1 and p21 protein levels were measured by western blotting analysis. Results: STZ administration decreased plasma insulin and increased fasting blood glucose in Sprague-Dawley (SD) rats (Fig. 1A and B). Adenovirus was transfected into the carotid artery in diabetic rats successfully at 14 days after balloon injury (representative microphotographs of fluorescent microscopy after adenovirus transfection, Fig. 1C). Ad-miR-24GFP increased the expression of miR-24 and suppressed VSMCs proliferation, neointimal hyperplasia in diabetic rats (Fig.2). Furthermore, the protein levels of PCNA, Wnt4, Dvl-1, b-catenin were strikingly up-regulated in diabetic rats carotid artery after balloon injury compared with Sham group. Interestingly, up-regulation of miR-24 significantly reduced the levels of these proteins through regulating the expressions of cell cycle genes Cyclin D1 and p21. Conclusion: We conclude that over-expression miR-24 could attenuate VSMCs proliferation and neointimal hyperplasia in the diabetic rat carotid artery injury model, which was possibly related to regulate the expressions of Cyclin D1 and p21 through Wnt4/Dvl-1/bcatenin signaling pathway. Disclosure of Interest: None Declared
knowledge and attitude scores (r¼0.224, p<0.001), as well as knowledge and practice scores (r¼0.114, p<0.001). There was no correlation between attitude and practice scores. Significant multivariate predictors of higher knowledge scores included female sex, higher level of education, higher attitude and practice scores and prior coronary artery disease. Significant multivariate predictors of higher attitude scores included higher level of education, higher knowledge scores and non-Indian ethnicity. Significant multivariate predictors of higher practice scores included male sex, Indian ethnicity, older age, higher knowledge score and hypertension. Males had significantly lower knowledge scores (85.88.0% vs 88.08.2%, p<0.001), lower attitude scores (91.49.4% vs 93.28.3%, p¼0.005) and higher practice scores (58.418.7% vs 55.119.3%, p¼0.013) than females. Conclusion: In our Asian cohort, knowledge of cardiovascular health plays a significant role in influencing attitudes and practices. There exist significant gender differences in KAP. Adopting gender-specific strategies for future public health campaigns could address the above gender differences. Disclosure of Interest: None Declared
underlying pathology (atherosclerosis, restenosis, radiation-induced arteriopathy, vasculitis), lesion location, and stenosis grade. Calcifications, long lesions (> 20 mm), and long balloon-expandable stents (> 20 mm) were more common in the fractured than in the non-fractured group (86.0% versus 43.0%, P < 0.001; 51.2% versus 20.3%, P < 0.001; 62.8% versus 40.5%, P ¼ 0.036, respectively). The restenosis and reintervention rates were significantly higher in patients with type III-V fractures compared to those with type I-II fractures (60.0% versus 3.6%, P < 0.001; 46.7% versus 3.6%, P < 0.001, respectively). Conclusion: Fractures frequently occur. Lesion and stent characteristics have an influence on fracture rate. Complex fractures increase the risk of restenosis. Disclosure of Interest: None Declared
PS319 Microrna-24 Attenuates Neointimal Hyperplasia in the Diabetic Rat Carotid Artery Injury Model By Inhibiting Wnt4 Signaling Pathway
PS314
J. Yang*1, Z.-X. Fan2, J. Yang1, J.-W. Ding2, C.-J. Yang2, P. Zeng2 Department of Cardiology, the First College of Clinical Medical Sciences, China Three Gorges University, Yichang 443000, Hubei Province, China, 2The First College of Clinical Medical Sciences, China Three Gorges University, Yichang, China 1
Rosuvastatin Dose-Dependently Causes Insulin Resistance and Increases Ambient Glycemia in Hypercholesterolemic Patients K. Koh*1 1 Gachon University Gil Medical Center, Incheon, Republic Of Korea Introduction: Genetic analysis from patients participated in the randomised trials reported that the increased risk of type 2 diabetes noted with statins is at least partially explained by HMG-coenzyme A reductase inhibition. Objectives: We investigated whether rosuvastatin dose-dependently decreases insulin sensitivity and increases ambient glycemia in hypercholesterolemic patients. Methods: This was a randomized, single-blind, placebo-controlled, parallel study. Age, sex, and body mass index were matched among groups. Forty-eight patients were given placebo, and 47, 48, and 47 patients given rosuvastatin 5,10, and 20 mg, respectively daily during a 2 month treatment period. Results: Placebo therapy did not significantly change insulin, adiponectin, glycated hemoglobin, and insulin sensitivity relative to baseline measurements. Rosuvastatin 5,10, and 20 mg dose-dependently and significantly increased insulin (median % changes; 16, 20, and 20%, respectively) and glycated hemoglobin levels (mean % changes; 2, 2, and 3%, respectively), and decreased adiponectin levels (mean % changes; 3, 9, and 14%, respectively) and insulin sensitivity (mean % changes; 2, 3, and 4%, respectively) after 2 months therapy when compared with either baseline (all P<0.05 by paired t-test). These effects with rosuvastatin 5,10, and 20 mg were significant when compared with placebo (P¼0.006 for insulin, P¼0.012 for glycated hemoglobin, P¼0.007 for adiponectin, and P¼0.002 for insulin sensitivity by ANOVA). Conclusion: Despite beneficial reductions in LDL cholesterol and apolipoprotein B, rosuvastatin treatment dose-dependently and significantly resulted in decreasing insulin sensitivity and increasing ambient glycemia by reducing adiponectin levels and increasing insulin levels in hypercholesterolemic patients. Disclosure of Interest: None Declared
Introduction: Diabetes mellitus (DM) is a worldwide health problem, in both the developed and developing countries. Long-term stimulus of hyperglycemia greatly increases the incidence of vascular restenosis (RS) after angioplasty (such as percutaneous coronary intervention, PCI). MicroRNA-24(miR-24) is low expression in diabetic rat carotid artery after balloon injury. However, the role of miR-24 in vascular system is unknown.
PS317 Incidence and Risk Factors for Subclavian Artery Stent Fracture E. Dósa*1, A. G. Hüttl1, A. Hüttl1, E. M. Végh1, B. Nemes1, K. Hüttl1 1 Heart and Vascular Center, Semmelweis University, Budapest, Hungary Introduction: Stenting is the preferred, minimally invasive treatment for subclavian artery (SA) steno-occlusive disease. Stent fractures in the SA have not been assessed in large cohorts. Objectives: To determine the prevalence of SA stent fractures, identify predisposing factors, and analyze their impact on restenosis development. Methods: 120 patients (71 women; mean age: 59.4+/-9.0 years) with symptomatic significant SA stenosis who underwent stenting between 2000 and 2012 at our Department have been included in the study. In 2014, patients were asked to return for a fluoroscopic examination of the implanted stents. Stent fractures were defined as type I: single-strut fracture, type II: > 2 strut fractures without deformation, type III: > 2 strut fractures with deformation, type IV: multiple strut fractures with acquired transection but without gap, and type V: multiple strut fractures with acquired transection with gap in the stent body. Color duplex scan has been used to monitor stent patency. In case of continuous variables t or Mann-Whitney U test, while in case of categorical variables chi-square or Fisher exact test were performed. Results: 122 stents have been deployed (balloon-expandable, n ¼ 94; self-expandable, n ¼ 28). The median follow-up time was 81.4 (41.1-113.7) months. 43 (35.2%) stent fractures were detected (type I-II, n ¼ 28; type III-V, n ¼ 15). The difference between the fractured (n ¼ 43) and non-fractured (n ¼ 79) groups was non-significant regarding the atherosclerotic risk factors (age > 70 years, female sex, smoking, hypertension, hyperlipidemia, diabetes mellitus, obesity, chronic kidney disease),
e68
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j
June, 2016
j
POSTER/WCC_2016-POSTERS
Methods: Group A ¼ 32 volunteers in the last year of Medical school was compared to Group B ¼ 24 volunteers of human sciences school. Both groups received theoretical and practical training in cardiopulmonary resuscitation and automated external defibrillator (AED) based on the methodology of Basic Life Support courses (BLS) from American Heart Association. At the end of the course, and after 30 and 60 days, all students were evaluated for their ability to perform CPR and the AED, using manikin ResusciAnne Skill Reporter - Laerdal Ò Company. The practical evaluation was recorded on DVD for later evaluation by experienced instructors that did not know the students. A checklist created by BLS practical evaluation for Health care providers was used. Previous trained students and those who did not agree to participate in the project were excluded. Results: In practical evaluation, human sciences students had lower score than medical students at 30 and 60 days (p<0,05). In practical evaluations at 30 to 60 days, human sciences students had lower scores in 60 days than in 30 days. Parameters like compression depth and ventilation rate were equal for both groups. Conclusion: Physiological understanding influence in maintaining the ability to perform CPR without significantly interference with the quality of compression or ventilation. Disclosure of Interest: None Declared PS324 Characterization of Bleeding and Thrombotic Events Dialysis Patients Who are Treated with Antithrombotic Agents C. M. Doria Murcia*1, R. M. Rodríguez Amaya1, C. L. Figueroa Pineda1 1 Universidad Industrial de Santander, Grupo GERMINA, Bucaramanga, Colombia
PS320 Cardiopulmonary Resuscitation Training Using Realtime Quantitative System A.-A. Augustine1, R. Subramaniam1, M. Timam1, A. Bungsu1, S. Y. Liau2, P. N. Arumuganathan*1, Y. W. Lee1, C. H. Yen1, G. Ma1, H. B. Liew1 1 Cardiology, 2Clinical Research Centre, Queen Elizabeth II Hospital, Kota Kinabalu, Malaysia Introduction: Cardiopulmonary resuscitation (CPR) is an important skillset for all healthcare workers (HCW) in the event of a cardiopulmonary arrest. Effective CPR requires training, to achieve and maintain adequate speed and depth of compression in the right position. Mannequin with immediate quantitative assessment can facilitate CPR training course, by providing real time feedback to both trainer and trainees. Objectives: To evaluate the effectiveness of realtime feedback mannequin in CPR training, by comparing pre and post-training. Methods: This was a single centre study (tertiary cardiac centre). Between 10th October 2014 to 4th September 2015, a total of 33 HCW underwent the CPR training sessions. CPR course began with a basic lecture regarding CPR standards, and demonstration of the mannequin. Pre-training assessment was conducted, and result feedback were immediate, followed by a practice on mannequins to improve speed and depth of compressions. After a period of practice, reassessment performed. Data collection was automated in the Laerdal mannequin system. Candidates were staff from the cardiology department. Data was entered into SPSS and analysed descriptively. Comparative analysis of variables pre versus post training was calculated using paired sample T Test. A two-tailed P-value < 0.05 was considered statistically significant. Results: Mean age was 26.3 years old, 66.7% female; majority of subjects were nurses 51.5%; average years of practice in medical field was 2.8 years. No significant differences in compression depth (53.76 vs 52.48), and hand position (93.6% vs 98.0%). However, there is notable improvement in compression rate (45.7% vs 68.3%) and recoil (62.27% vs 73.97%, p value > 0.05). There was no correlation between years of service and total score (r ¼ 0.063). Conclusion: Our study showed there was no significant change of results in pre and post CPR training amongst HCW who underwent training using the real time feedback mannequin system. Future use of quantitative CPR assessment in training of non-HCW, example lay public would be needed to further establish the usefulness and effectiveness of such technology. Disclosure of Interest: None Declared
Introduction: The End-Stage Renal Disease ESRD, is one of the diseases fastest growing worldwide turn is classified as a public health problem due to increased morbidity, mortality and represents high costs to the system of health. Moreover, current evidence has shown that cardiovascular risk scales used in the general population, are not fully extrapolated to the group of patients with ESRD and that in several cases the results of the studies have been inconclusive in determining whether there is a increased risk of bleeding or thromboembolic complications. Objectives: The aim of the study was to determine sociodemographic and clinical aspects of dialysis patients who are treated with antithrombotic agents and determine the frequency of thrombotic events and bleeding from a historical cohort during the period from 2009 through 2013. Methods: An observational study was conducted and a historical cohort, which included patients with ESRD over 18 years, most dialysis at 90 days, treatment modality dialysis, automated peritoneal dialysis and continuous ambulatory peritoneal dialysis, they had prescription studied antithrombotic agent and belonging to a renal clinic Renal Therapy Service (RTS) in ColombiaÒ, in the time period between January 1, 2009 at 31 December 2013. We excluded those with liver disease or other concomitant disorders coagulation. The sample size calculation was probabilistic, through a scheme simple random sampling treatment groups. It was taken to the execution of the study endorsed by the ethics committee of the Industrial University of Santander. Results: A final sample of 965 patients was achieved, mainly male (61%) with a mean age of 61.8 13.5 years. Diabetes (46.1%) was the major cause of renal disease followed by hypertension (24.6%). In addition, 62% of patients had started dialysis as an emergency. Also, 159 patients had a thrombotic or hemorrhagic event, 11.4% had a thrombotic event and 6.5% experienced a bleeding event. Table 1 attached distribution of bleeding and thrombotic events according to the group of drugs received at the start of dialysis treatment in the cohort is appreciated.
PS321 Effect of Physiological Understanding In CPR Skill Retention H. C. Miotto1, I. Z. Miotto1, R. R. Oliveira1, M. D. C. V. Moreira*1 Internal Medicine, Federal University of Minas Gerais, Belo Horizonte, Brazil
1
Introduction: Retention of CPR skills deteriorates very rapidly. We made the hypothesis that lay rescuer loses their ability sooner than health provider. Objectives: Assess whether the level of physiological understanding of cardiopulmonary resuscitation may influence the learning and retention of motor skills.
GHEART Vol 11/2S/2016
j
June, 2016
j
POSTER/WCC_2016-POSTERS
Conclusion: In light of the data and still having a higher average retail creatinine and hemoglobin, the group of drugs with less presence of thrombotic and bleeding events was the LMWH, there was also an increased risk of bleeding events tormbóticos present. Disclosure of Interest: None Declared
e69
POSTER ABSTRACTS
Objectives: To explore whether over-expression miR-24 could attenuate neointimal formation in streptozotocin (STZ) induced diabetic rats. Methods: Adenovirus (Ad-miR-24-GFP) was used to delivery gene (miR-24) to injury carotid artery in diabetic rats. The degree of neointimal hyperplasia was evaluated by hematoxylin-eosin (HE) staining. Vascular smooth muscle cells (VSMCs) proliferation in the neointima of injury artery was examined by immunostaining for proliferating cell nuclear antigen (PCNA). The mRNA levels of miR-24 and cell cycle-associated molecules (Cyclin D1, p21) were determined by quantitative real-time PCR (qRT-PCR) analysis. PCNA, Wnt4, Disheveled-1(Dvl-1), b-catenin, Cyclin D1 and p21 protein levels were measured by western blotting analysis. Results: STZ administration decreased plasma insulin and increased fasting blood glucose in Sprague-Dawley (SD) rats (Fig. 1A and B). Adenovirus was transfected into the carotid artery in diabetic rats successfully at 14 days after balloon injury (representative microphotographs of fluorescent microscopy after adenovirus transfection, Fig. 1C). Ad-miR-24GFP increased the expression of miR-24 and suppressed VSMCs proliferation, neointimal hyperplasia in diabetic rats (Fig.2). Furthermore, the protein levels of PCNA, Wnt4, Dvl-1, b-catenin were strikingly up-regulated in diabetic rats carotid artery after balloon injury compared with Sham group. Interestingly, up-regulation of miR-24 significantly reduced the levels of these proteins through regulating the expressions of cell cycle genes Cyclin D1 and p21. Conclusion: We conclude that over-expression miR-24 could attenuate VSMCs proliferation and neointimal hyperplasia in the diabetic rat carotid artery injury model, which was possibly related to regulate the expressions of Cyclin D1 and p21 through Wnt4/Dvl-1/bcatenin signaling pathway. Disclosure of Interest: None Declared