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Regulation of the mRNA transcription and translation processes of the ambisense S RNA of punta toro phlebovirus (bunyaviridae)
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REGULATIGN OF THE mRNA TRANSCRIPTION AND TRANSLATION PROCESSES OF THE AMBISENSE S RNA OF PUNTA TORO PHLEBOVIRUS (BUNYAVIRIDAE) DAVID H.L. BISHOP, YOSHIHARU MATSUURA and TAKESHI IHARA, Institute of Virology, Mansfield Road, Oxford OX1 3SR, U.K.
NERC
The S RNA species of Punta Toro (PT) phlebovirus has been cloned into DNA and sequenced providing evidence for its ambisense coding strategy, Viralcomplementary, subgenomie, mRNA species that code for the viral N protein have been identified (using appropriate singlestranded DNA probes) in virus infected cells incubated in the presence or absence of inhibitors of protein synthesis (e.g., 100 pg puromyein, or cyeloheximide per ml of culture medium). Similar data for primary tr&nseription of snowshoe hare bunyavirus have been obtained. The PT N mRNA species have short, heterogeneous, non-viral, 5’ end sequences, like those of bunyav~ruses and orthomyxoviruses. Viral-sense, subgenomic, PT mRNA species that code for a putative nonstructural protein, NSS, have been found only in ceils in which protein synthesis occurs, i.e., only in the absence of puromycin or eycloheximide. These data indicate that replication of PT viral S RNA is required before NSS mRNA can be made and that the N and NSS mRNA species are regulated independently of each other.
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REGULATIGN OF THE mRNA TRANSCRIPTION AND TRANSLATION PROCESSES OF THE AMBISENSE S RNA OF PUNTA TORO PHLEBOVIRUS (BUNYAVIRIDAE) DAVID H.L. BISHOP, YOSHIHARU MATSUURA and TAKESHI IHARA, Institute of Virology, Mansfield Road, Oxford OX1 3SR, U.K.
NERC
The S RNA species of Punta Toro (PT) phlebovirus has been cloned into DNA and sequenced providing evidence for its ambisense coding strategy, Viralcomplementary, subgenomie, mRNA species that code for the viral N protein have been identified (using appropriate singlestranded DNA probes) in virus infected cells incubated in the presence or absence of inhibitors of protein synthesis (e.g., 100 pg puromyein, or cyeloheximide per ml of culture medium). Similar data for primary tr&nseription of snowshoe hare bunyavirus have been obtained. The PT N mRNA species have short, heterogeneous, non-viral, 5’ end sequences, like those of bunyav~ruses and orthomyxoviruses. Viral-sense, subgenomic, PT mRNA species that code for a putative nonstructural protein, NSS, have been found only in ceils in which protein synthesis occurs, i.e., only in the absence of puromycin or eycloheximide. These data indicate that replication of PT viral S RNA is required before NSS mRNA can be made and that the N and NSS mRNA species are regulated independently of each other.
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