315
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Determination of anions in human and animal tear fluid and blood serum by ion chromatography by R. Salas-Auvert, J. Colmenarez, H. de Ledo Mr M. Colina, E. Gutierrez, A. Bravo, L. Soto and S. Azuero (Maracaibo, Venezuela) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
183
Determination of amino acids in biomass and protein samples by microwave hydrolysis and ion-exchange chromatography by L. Joergensen and H.N. Thestrup (Odense M, Denmark) ............................. 421 Direct determination of seleno-amino acids in biological tissues by anion-exchange separation and electrochemical detection by S. Cavalli (Pieve Emanuele MI, Italy) and N. Cardellicchio (Taranto TA, Italy) . . . . . . . . . . . . . . . . . 429 Nitric oxide in biological fluids: analysis of nitrite and nitrate by high-performance ion chromatography by S.A. Everett, M.F. Dennis, G.M. Tozer,V.E. Prise, P. Wardman and M.R.E. Stratford (Northwood, UK)
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437
Ion chromatography as potential reference methodology for the determination of total sodium and potassium in human serum by L.M. Thienpont and J.E. Van Nuwenborg (Ghent, Belgium) and D. St6ckl (Dfisseldorf, Germany) . . . . . . . . 443 Plasma level determination of 1,4-butanedisulphonate by ion chromatography and conductimetric detection by E. Moro, M. De Angelis and B. Fugazza (Liscate (MI), Italy) . . . . . . . . . . . . . . . . . . . . . . . . . . .
451
Analysis of the acidic microenvironment in murine tumours by high-performance iun chromatography by M.R,L. Stratford, C.S. Parkins, S.A. Everett and M.F. Dennis (Northwood, UK), M. Stubbs (London, UK) and S.A. Hill (Northwood, UK) .............. ~ ............................... 459 CAG triplet analysis in families with androgen insensitivity syndrome by capillary electrophoresis in polymer networks by C. Gelfi, P.G. Righetti, F. Leoncini,V. Brunelli, P. Carrera and M, Ferrari (Milan, Italy) . . . . . . . . . . . . .
463
V o l u m e 7(17
Current trends in molecular recognition and bioseparation (Review) by C. Jones, A. Patel, S. Griffin, J. Martin, P. Young, K. O'Donncll, C. Sil~erman, T. Porter and 1. Chaiken (King of Prussia, PA, USA) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3
Preparative chromatographic separations in pharmaceutical, diagnostic, and biotechnology industries: current and future trends (Review) by G. Sofer (Piscataway, NJ, USA) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
23
Antibiotics as low-molecular-mass displacers in ion-exchange displacement chromatography by A. Kundu, S. Vunnum and S.M. Cramer (Troy, NY, USA) . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
57
Capability of a polymeric C~. stationary phase to resolve c i s - t r a n s carotenoid isomers in reversed-phase liquid chromatography by C. Emenhiser (Raleigh, NC, USA), L.C. Sander (Gaithersburg, MD, USA) and S.J. Schwartz (Raleigh, NC, USA) .......................................................... 205 Purification of antibody Fab fragments by cation-exchange chromatography and pH gradient elution by R. Mhatre, W. Nashabeh, D. Schmalzing, X. Yao, M. Fuchs, D. Whitney and F. Regnier (Framingham, MA, USA) .......................................................... 225 Peptide chiral purity determination: hydrolysis in deuterated acid, derivatization with Marfey's reagent and analysis using high-performance liquid chromatography-electrospray ionization-mass spectrometry by D.R. Goodlett, P.A. Abuaf, P.A. Savage, K.A. Kowalski, T K . Mukherjee, J.W. Tolan, N. Corkum, G. Goldstein and J.B. Crowther (Annandale, NJ, USA) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 233 Simultaneous determination of enalapril, felodipine and their degradation products in the dosage formulation by reversedphase high-performance liquid chromatography using a Spherisorb C, column by X.-Z. Qin, J. DeMarco and D.P. Ip (West Point, PA, USA) ............................ 245
316
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396
708
Preparation of cDNA-silica using reverse transcriptase and its DNA sequence determination by H.W. Jarrett (Memphis, TN, USA) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
13
Determination of aflatoxins in airborne dust from feed factories by automated immunoaffinity column clean-up and liquid chromatography by A. Kussak, B. Andersson and K. Andersson (Ume~, Sweden) . . . . . . . . . . . . . . . . . . . . . . . . . . .
55
Development and monitoring of purification process for nerve growth factor fusion antibody by A.J. Hunt, P.D. Lynch, T. Londo, P. Dimond, N.F. Gordon and T. McCormack (Framingham, MA, USA), A. Schutz, M. Percoskie, X. Cao, J.P. McGrath and S. Putney (Cambridge, MA, USA) and R.A. Hamilton (Framingham, MA, USA) ...............................................
61
Ion-pair chromatographic method for the analysis of mafenide acetate by A.K. Dash and J.S. Harrison (Omaha, NE, USA) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
83
Separation of enantiomers on a pepsin-bonded column by J. Haginaka, Y. Miyano, Y. Saizen, C. Seyama and T. Murashima (Nishinomiya, Japan) . . . . . . . . . . . . . . Analysis of illicit amphetamine seizures by capillary electrophoresis by V.C. Trenerry (Seaton, Australia), J. Robertson (Weston, Australia) and R.J. Wells (Pymhle, Australia)
161 .....
169
Optimization of the determination of amino acids in parenteral solutions by high-performance liquid chromatography with precolumn derivatization using 9-fluorenylmethyl chloroformate by B. Carratfl, C. Boniglia and G. Bellomonte (Rome, Italy) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203 Evaluation of solid-phase extraction procedures in peptide analysis by T. Herraiz and V. Casal (Madrid, Spain) . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . .
209
Enantiomeric separation of a thiazole derivative by high-performance liquid chromatography and micellar electrokinetic chromatography by R. Furuta and T. Doi (Osaka, Japan) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 245 Chiral separations of/3-blocking drug substances using chiral stationary phases by J. Ekelund, A. van Arkens, K. BrOnnum-Hansen, K. Fich, L. Olsen and P.V. Petersen (BrOnshoj, Denmark)
. . 253
Diode laser-induced fluorescence detection in capillary electrophoresis after pre-column derivatization of amino acids and small peptides by A.J.G. Mank (Amsterdam, Netherlands) and E.S. Yeung (Ames, IA, USA) . . . . . . . . . . . . . . . . . . . . 309
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Microemulsion electrokinetic chromatography with different organic modifiers: separation of water- and lipid-soluble vitamins by R.L. Boso and M.S. Bellini (Verona, Italy) and 1. Mik~ik and Z. Deyl (Prague, Czech Republic) . . . . . . . . .
I1
Enantiomeric separation by capillary electrophoresis using a soluble neutral ~-cyclodextrin polymer by B.A. Ingelse (Rome, Italy), F.M. Everaerts (Eindhoven, Netherlands) and C. Desiderio and S. Fanali (Rome, Italy) ..........................................................
89
Capillary zone electrophoresis of serum proteins: study of separation variables by V. Dolnfk (Brno, Czech Republic) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
99
Capillary electrophoresis of hair proteins modified by alcohol intake in laborator~ rats by D. Jelinkovfi, Z. Deyl and I. Mik~ik (Prague, Czech Republic) and F. Tagliaro (Verona, Italy) Haemoglobin analysis by capillary zone electrophoresis by A. ~ahin, Y.R. La[eli and R. Ortancd (Ankara, Turkey)
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111
..............................
121
Detection of traces of a trisulphide derivative in the preparation of a recombinant truncated interleukin-6 mutein by J. Breton, N. Avanzi, B~ Valsasina, L. Sgare[la, A. La Fiura, U. Bremc and G. Orsini (Nerviano, Italy) and E. Wenisch and P.G. Righetti (Cosenza, Italy) ......................................
135
Modification of a tunable UV-visible capillary eleetrophoresis detector for simultaneous absorbance and fluorescence detection: profiling of body fluids for drugs and endogenous compounds by J. Caslavska (Berne, Switzerland), E. Gassmann (Basle, Switzerland) and W. Thormann (Berne, Switzerland) . . 147 Chiral separation of deprenyl and its major metabolites using cyclodextrin-modified capillary zone electrophoresis by 1~. Sz6k5 and K. Magyar (Budapest, Hungary) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Stability measurements of antisense oligonucleotides by capillary gel electrophoresis by G.J.M. Bruin, K.O. B6rnsen, D. Hfisken, E. Gassmann, H.M. Widmer and A. Paulus (Basel, Switzerland)
171 . . , 181
High-performance liquid chromatographic and capillary electrophoretic determination of free nicotinic acid in human plasma and separation of its metabolites by capillary electrophoresis by P.K. ZarzyckL P. KowalskL J. Nowakowska and H. Lamparczyk (Gdafisk, Poland) . . . . . . . . . . . . . . . . 203 Development of an analytical reversed-phase high-performance liquid chromatography assay for transforming growth factor
t33 by W.J. Lambert and G,F. Stamper (Groton, CT, USA)
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
249
Improved electrochemical detection of diuretics in high-performance liquid chromatographic analysis by postcolumn on-line photolysis by M. Macher and R. Wintersteiger (Graz, Austria) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257 Separation of enantiomers of benzodiazepines on the Chiral--AGP column by 1. Fitos, J. Visy and M. Simonyi (Budapest, Hungary) and J. Hermansson (Hfigersten, Sweden)
.........
265
Analysis of trichloroacetic acid in the urine of workers occupationally exposed to trichloroethylenc by capillary gas chromatography by G.E. O'Donnell, A. Juska, R. Geyer, M. Faiz and S. Stalder (Thornleigh, Australia) . . . . . . . . . . . . . . . 313 Volume 710
Separation of ~:P-labeled 3',5'-bisphosphate nucleotides of polycyclic aromatic hydrocarbon anti-diol-epoxides and deriwlrives by D.-J. Zhan, D. Herreno-Saenz, L.-H. Chiu and LS. Von Tungein (Jefferson, AR, USA), Yu.-S. Wu (Taichung, Taiwan), J. Lewtas (Triangle Park, NC, USA) and P.P Fu (Jefferson, AR, USA) .................. 149 High-performance liquid chromatographic method for the separation of the optical isomers of 7,y'-di-terL-butyl-.~,~-'/carboxyglutamic acid and D,L-y-carboxyglutamic acid by A. P6ter, C. Somlai and B. Penke (Szeged, Hungary) ..............................
297
Enantiomeric separation of amphetamine related drugs by capillary zone electrophoresis using native and derivatized /3-cyclodextrin as chiral additives by S. Cladrowa-Runge, R. Hirz, E. Kenndler and A. Rizzi (Vienna, Austria) . . . . . . . . . . . . . . . . . . . . . 339 Measurement of desmosine and isodesmosine by capillary zone electrophoresis by P. Giummelly. B. Botton, R. Friot, D. Prima-Putra and J. Atkinson (Nancy, France)
...............
357