- Email: [email protected]
Relationship of bone state and hormonal status in men
Abstracts / Bone 44 (2009) S339–S450
visits correlated (p < 0.005). Changes in HCY and folate between groups did not differ significantly (p = 0.13, p = 0.31). Neither did changes between visits correlate with LBP (p > 0.9). Changes in B12 correlated with LBP between 1st and 3rd (r = −0.35, p = 0.001) and 2nd and 3rd visit (r = −0.24, p = 0.026). In gr.1 B12 increased significant at 3rd visit compared to 1st and 2nd visit (p < 0.001). In the two other groups B12 did not changes significant over time. Changes in B12 did not correlate to neither Hcy nor folate at any visit (p > 0.3) There were no significant correlations between changes in BMD at any points and changes in Hcy, folate or B12 in the three groups separately (p > 0.1) during the study. Conclusion: We found significant relations between changes in regional and total BMD over time and in HCY, Folate and B12. However we found no relations between BMD changes and changes in vitamin B status. Conflict of interest: None declared. doi:10.1016/j.bone.2009.03.207
P296 Deficiency of 25-hydroxyvitamin D3 in a department of gastroenterology. A descriptive cross-sectional study U.C. Banga,*, S. Sembb, I. Nordgaard-Lassenb, J.B. Jensena a Osteoporosis, Hvidovre Hospital, Hvidovre, Denmark b Gastroenterology, Hvidovre Hospital, Hvidovre, Denmark Objective: The aim of this descriptive cross-sectional study is to describe 25-hydroxyvitamin D (25(OH)D3) deficiency in patients admitted to a department of gastroenterology. Materials and methods: After informed consent blood samples were collected in May and June 2008 and analyzed in the hospital laboratory. The concentration of 25(OH)D3 was defined as insufficient when < 50 nmol/l, deficient when <25 nmol/l and severe deficient when <12.5 nmol/l. Results: 146 patients with a mean age of 55 years (range 16 to 93) were included. 25(OH)D3 was sufficient in 47%, insufficient in 29%, deficient in 12% and severe deficient in 11% of the population. Participants taking vitamin D supplementation had higher 25(OH)D3 levels and participants with alcoholism or cirrhosis had lower levels of 25(OH)D3. Participants with severe 25(OH)D3 deficiency had higher levels of tAP and PTH. Conclusion: The prevalence of 25(OH)D3 deficiency is high in a hospitalized population at a department of gastroenterology. We recommend measuring the level of 25(OH)D3 and PTH in patients with chronic diseases especially inflammatory bowel disease, alcoholism or cirrhosis. Conflict of interest: None declared. doi:10.1016/j.bone.2009.03.208
P297 Relationship of bone state and hormonal status in men V. Povoroznyuk*, T. Orlyk, Y. Kreslov Department of Clinical Physiology and Pathology of Locomotor Apparatus, Institute of Gerontology AMS Ukraine, Kyiv, Ukraine The aim of the study was to determine the relationship of hormonal status and bone state in men. Materials and methods: We have examined 96 men aged from 30 to 79 years (M ± m): age — 54.4 ± 1.3 years; height — 1.75 ± 0.01 m; weight — 84.9 ± 1.5 kg), divided them into age dependent subgroups 30–49 (n = 36; age — 41.2 ± 1.2 years) and 50–79 years (n = 60; age — 64.4 ± 1.1 years). Levels of testosterone (Test, nmol/l) and sex
S365
hormone-binding globulin (SHBG, nmol/l) were determined by means of chemiluminescent immunoanalysis method. The bone mineral density (BMD, g/cm2) was evaluated for the total body, spine (L1–L4), femur (neck, trochanter and total) and radius (ultradistal, 33% and total) using dual energy X-ray absorptiometry by the Prodigy instrument (GE Medical systems, 2005). Results: The correlation analysis of age dependent sub-groups: in the group of 30–49 years there is a positive correlation between test and BMD ultradistal radius (r = 0.49, p < 0.05), along with the negative correlation between SHBG and total body in the group of 50–79 years (r = −0.31, p < 0.05). In the group of 60–79 years (n = 38; age — 69.7 ± 1.0 years) we have found a negative correlation between SHBG and total body (r = −0.60, p < 0.001), SHBG and trochanter (r = −0.47, p < 0.05), SHBG and total femur (r = −0.48, p < 0.05). Patients of the 50–79 year age group with normal bone, osteopenia and osteoporosis were chosen in correspondence to the WHO criteria. For analysis' sake, we have joint the osteopenia and osteoporosis patients. Normal mineral density of lumbar spine was found in 83.3%, osteopenia and osteoporosis — 17.7%, while in total femur — 75% and 25% respectively. SHBG in normal femur BMD subgroup (41.1 ± 2.6) was considerably lower than in osteopenia and osteoporosis subgroup (54.4 ± 5.6, p < 0.05). Conclusion: We have revealed a positive correlation between testosterone levels and ultradistal radius BMD and negative correlation between SHBG and total body BMD in patients of 50–79 year age group, trochanter and total femur in patients of 60–79 year age group. Conflict of interest: None declared. doi:10.1016/j.bone.2009.03.209
P298 Cumulative influence of calcemia and vitamin D on mouse dentition V.J.M. Descroixa,*, F. Lezota, D. Hottona, S. Katob, A. Berdala a Equipe 5 Physiologie Orale Moléculaire, Inserm UMRS 872 Centre de Recherche des Cordeliers, Paris, France b The Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan Background: Vitamin D pathway bio-inactivation and induced rickets may result from nutritional deficiency and mutations in the vitamin D receptor gene. The spectrum of dental phenotype in vitamin D-deficient rats raised under normocalcemic and normophosphatemic condition during two generations include crown dysmorphologies, enamel dysplasias, dentin defects and enamel protein level modulations. In order to analyse cumulative influences of (1) the vitamin D receptor per se, (2) calcium and phosphate homeostasis and (3) maternal environment, the dental phenotype was analysed in vitamin D receptor null mutant mice (VDR −/−) from 2 to 56 days. Methods: Heterozygous VDR +/− and homozygous VDR −/− mice were mated in order to obtain first VDR −/− and second (2nd) generation VDR −/− mouse pups, respectively. Normocalcemic and normophosphatemic (N) mice were generated by adding 20% lactose, 4% calcium and 1,26% phosphate to the control diet and compared to hypocalcemic and hypophosphatemic (H) mice. Results: Enamel protein (amelogenin, ameloblastin, enamelin) levels (light cycling-RT-PCR and immuno-localizations) were differentially modulated in incisor dental epithelial cells and enamel of H and N VDR−/− mice when compared to their corresponding control VDR +/+ and VDR +/− littermates. Morphological comparison of H and N VDR −/− mice enabled to show that molar root resorptions and increased number of tartrate-resistant acid phosphatase-positive osteoclasts in the alveolar bone were not directly related to the vitamin D receptor in dental cells, but to affected homeostasis. The most prominent structural defects (notably crown dysmorphologies and osteodentin formation) were recorded in 2nd VDR −/− mice.
visits correlated (p < 0.005). Changes in HCY and folate between groups did not differ significantly (p = 0.13, p = 0.31). Neither did changes between visits correlate with LBP (p > 0.9). Changes in B12 correlated with LBP between 1st and 3rd (r = −0.35, p = 0.001) and 2nd and 3rd visit (r = −0.24, p = 0.026). In gr.1 B12 increased significant at 3rd visit compared to 1st and 2nd visit (p < 0.001). In the two other groups B12 did not changes significant over time. Changes in B12 did not correlate to neither Hcy nor folate at any visit (p > 0.3) There were no significant correlations between changes in BMD at any points and changes in Hcy, folate or B12 in the three groups separately (p > 0.1) during the study. Conclusion: We found significant relations between changes in regional and total BMD over time and in HCY, Folate and B12. However we found no relations between BMD changes and changes in vitamin B status. Conflict of interest: None declared. doi:10.1016/j.bone.2009.03.207
P296 Deficiency of 25-hydroxyvitamin D3 in a department of gastroenterology. A descriptive cross-sectional study U.C. Banga,*, S. Sembb, I. Nordgaard-Lassenb, J.B. Jensena a Osteoporosis, Hvidovre Hospital, Hvidovre, Denmark b Gastroenterology, Hvidovre Hospital, Hvidovre, Denmark Objective: The aim of this descriptive cross-sectional study is to describe 25-hydroxyvitamin D (25(OH)D3) deficiency in patients admitted to a department of gastroenterology. Materials and methods: After informed consent blood samples were collected in May and June 2008 and analyzed in the hospital laboratory. The concentration of 25(OH)D3 was defined as insufficient when < 50 nmol/l, deficient when <25 nmol/l and severe deficient when <12.5 nmol/l. Results: 146 patients with a mean age of 55 years (range 16 to 93) were included. 25(OH)D3 was sufficient in 47%, insufficient in 29%, deficient in 12% and severe deficient in 11% of the population. Participants taking vitamin D supplementation had higher 25(OH)D3 levels and participants with alcoholism or cirrhosis had lower levels of 25(OH)D3. Participants with severe 25(OH)D3 deficiency had higher levels of tAP and PTH. Conclusion: The prevalence of 25(OH)D3 deficiency is high in a hospitalized population at a department of gastroenterology. We recommend measuring the level of 25(OH)D3 and PTH in patients with chronic diseases especially inflammatory bowel disease, alcoholism or cirrhosis. Conflict of interest: None declared. doi:10.1016/j.bone.2009.03.208
P297 Relationship of bone state and hormonal status in men V. Povoroznyuk*, T. Orlyk, Y. Kreslov Department of Clinical Physiology and Pathology of Locomotor Apparatus, Institute of Gerontology AMS Ukraine, Kyiv, Ukraine The aim of the study was to determine the relationship of hormonal status and bone state in men. Materials and methods: We have examined 96 men aged from 30 to 79 years (M ± m): age — 54.4 ± 1.3 years; height — 1.75 ± 0.01 m; weight — 84.9 ± 1.5 kg), divided them into age dependent subgroups 30–49 (n = 36; age — 41.2 ± 1.2 years) and 50–79 years (n = 60; age — 64.4 ± 1.1 years). Levels of testosterone (Test, nmol/l) and sex
S365
hormone-binding globulin (SHBG, nmol/l) were determined by means of chemiluminescent immunoanalysis method. The bone mineral density (BMD, g/cm2) was evaluated for the total body, spine (L1–L4), femur (neck, trochanter and total) and radius (ultradistal, 33% and total) using dual energy X-ray absorptiometry by the Prodigy instrument (GE Medical systems, 2005). Results: The correlation analysis of age dependent sub-groups: in the group of 30–49 years there is a positive correlation between test and BMD ultradistal radius (r = 0.49, p < 0.05), along with the negative correlation between SHBG and total body in the group of 50–79 years (r = −0.31, p < 0.05). In the group of 60–79 years (n = 38; age — 69.7 ± 1.0 years) we have found a negative correlation between SHBG and total body (r = −0.60, p < 0.001), SHBG and trochanter (r = −0.47, p < 0.05), SHBG and total femur (r = −0.48, p < 0.05). Patients of the 50–79 year age group with normal bone, osteopenia and osteoporosis were chosen in correspondence to the WHO criteria. For analysis' sake, we have joint the osteopenia and osteoporosis patients. Normal mineral density of lumbar spine was found in 83.3%, osteopenia and osteoporosis — 17.7%, while in total femur — 75% and 25% respectively. SHBG in normal femur BMD subgroup (41.1 ± 2.6) was considerably lower than in osteopenia and osteoporosis subgroup (54.4 ± 5.6, p < 0.05). Conclusion: We have revealed a positive correlation between testosterone levels and ultradistal radius BMD and negative correlation between SHBG and total body BMD in patients of 50–79 year age group, trochanter and total femur in patients of 60–79 year age group. Conflict of interest: None declared. doi:10.1016/j.bone.2009.03.209
P298 Cumulative influence of calcemia and vitamin D on mouse dentition V.J.M. Descroixa,*, F. Lezota, D. Hottona, S. Katob, A. Berdala a Equipe 5 Physiologie Orale Moléculaire, Inserm UMRS 872 Centre de Recherche des Cordeliers, Paris, France b The Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan Background: Vitamin D pathway bio-inactivation and induced rickets may result from nutritional deficiency and mutations in the vitamin D receptor gene. The spectrum of dental phenotype in vitamin D-deficient rats raised under normocalcemic and normophosphatemic condition during two generations include crown dysmorphologies, enamel dysplasias, dentin defects and enamel protein level modulations. In order to analyse cumulative influences of (1) the vitamin D receptor per se, (2) calcium and phosphate homeostasis and (3) maternal environment, the dental phenotype was analysed in vitamin D receptor null mutant mice (VDR −/−) from 2 to 56 days. Methods: Heterozygous VDR +/− and homozygous VDR −/− mice were mated in order to obtain first VDR −/− and second (2nd) generation VDR −/− mouse pups, respectively. Normocalcemic and normophosphatemic (N) mice were generated by adding 20% lactose, 4% calcium and 1,26% phosphate to the control diet and compared to hypocalcemic and hypophosphatemic (H) mice. Results: Enamel protein (amelogenin, ameloblastin, enamelin) levels (light cycling-RT-PCR and immuno-localizations) were differentially modulated in incisor dental epithelial cells and enamel of H and N VDR−/− mice when compared to their corresponding control VDR +/+ and VDR +/− littermates. Morphological comparison of H and N VDR −/− mice enabled to show that molar root resorptions and increased number of tartrate-resistant acid phosphatase-positive osteoclasts in the alveolar bone were not directly related to the vitamin D receptor in dental cells, but to affected homeostasis. The most prominent structural defects (notably crown dysmorphologies and osteodentin formation) were recorded in 2nd VDR −/− mice.