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Response to the Letter to the Editor regarding: Middle ear fluid characteristics in pediatric otitis media with effusion
International Journal of Pediatric Otorhinolaryngology 77 (2013) 453–454
Contents lists available at SciVerse ScienceDirect
International Journal of Pediatric Otorhinolaryngology journal homepage: www.elsevier.com/locate/ijporl
Letter to the Editor Letter to the Editor regarding ‘‘Middle ear fluid characteristics in otitis media with effusion: Concern for fluid analysis’’ Sir, The recent report on middle ear fluid characteristics in otitis media with effusion is very interesting [1]. Dodson et al. concluded that ‘‘The best mucociliary transportability was measured in mucopurulent effusions [1]’’. Indeed, there are many concerns on body fluid analysis including to middle ear fluid analysis in laboratory medicine. As Dodson et al. observed in their experiment, the transportability was different in the group with the first and repeated procedures. It should be noted that the fluid collection procedure is referred as a pre-analytical phase in laboratory quality cycle. The good collection technique is very important. In any analysis, the error in pre-analytical phase especially for the specimen collection is very high and this can result in aberration of final laboratory results [2]. To perform a procedure for middle ear fluid collection, the practical guideline has to be used and strictly followed.
you for these kind remarks. We support his observation that specimen collection and preservation are as crucial as the analytic techniques employed for obtaining accurate data. This is particularly important when measuring the biophysical properties of small volumes of secretions as shear forces associated with collection, or degradation associated with inappropriate storage can alter the biophysical and transport properties. We have developed a specific procedure for collection and preservation of these specimens and would be happy to share these robust techniques with readers interested in the collection and analysis of airway secretions. Bruce K. Rubin Department of Pediatrics, Virginia Commonwealth University School of Medicine, Children’s Hospital of Richmond at VCU, 1001 East Marshall Street, PO Box 980646, Richmond, VA 23298 USA Randall Cohen Private Practice, Tucson, Az, USA
References [1] K.M. Dodson, R.S. Cohen, B.K. Rubin, Middle ear fluid characteristics in pediatric otitis media with effusion, Int. J. Pediatr. Otorhinolaryngol. September (2012), http://dx.doi.org/10.1016/j.ijporl.2012.09.005, pii:S0165-5876(12)00511-3 [Epub ahead of print]. [2] V. Wiwanitkit, Types and frequency of preanalytical mistakes in the first Thai ISO 9002:1994 certified clinical laboratory, a 6-month monitoring, BMC Clin. Pathol. 1 (1) (2001) 5. a
Viroj Wiwanitkita,b,c,d,* Wiwanitkit House, Bangkhae, Bangkok, Thailand b Hainan Medical University, China c University of Nis, Serbia d Joseph Ayobabalola University, Nigeria
Kelley M. Dodson* Department of Otolaryngology, Virginia Commonwealth University Health System, Children’s Hospital of Richmond at VCU, USA *Corresponding author. Tel.: +1 804 828 3965; fax: +1 804 828 5779 E-mail address: [email protected] (K.M. Dodson) 8 November 2012
http://dx.doi.org/10.1016/j.ijporl.2012.11.017
*Correspondence at: Wiwanitkit House, Bangkhae, Bangkok, Thailand. Tel.: +66 24132436 E-mail address: [email protected] (V. Wiwanitkit) 6 October 2012
Letter to the Editor
http://dx.doi.org/10.1016/j.ijporl.2012.11.015
Response to the Letter to the Editor
Response to the Letter to the Editor regarding ‘‘Pie-slice tympanoplasty for transcanal removal of small congenital cholesteatoma’’ Dear Editor,
Response to the Letter to the Editor regarding: Middle ear fluid characteristics in pediatric otitis media with effusion To the Editor, We are pleased that Professor Wiwanitkit is enthusiastic about this manuscript and has found this information valuable. Thank 0165-5876/$ – see front matter ß 2012 Elsevier Ireland Ltd. All rights reserved.
We thank Dr. Johannes Borgstein for the comments on our article, ‘‘Pie-slice tympanoplasty for transcanal removal of small congenital cholesteatoma’’. Below are our responses to the three points raised by Dr. Borgstein: Response 1: We do not believe that these were acquired cholesteatomas based on location, size, healthy, mastoid, etc. We
Contents lists available at SciVerse ScienceDirect
International Journal of Pediatric Otorhinolaryngology journal homepage: www.elsevier.com/locate/ijporl
Letter to the Editor Letter to the Editor regarding ‘‘Middle ear fluid characteristics in otitis media with effusion: Concern for fluid analysis’’ Sir, The recent report on middle ear fluid characteristics in otitis media with effusion is very interesting [1]. Dodson et al. concluded that ‘‘The best mucociliary transportability was measured in mucopurulent effusions [1]’’. Indeed, there are many concerns on body fluid analysis including to middle ear fluid analysis in laboratory medicine. As Dodson et al. observed in their experiment, the transportability was different in the group with the first and repeated procedures. It should be noted that the fluid collection procedure is referred as a pre-analytical phase in laboratory quality cycle. The good collection technique is very important. In any analysis, the error in pre-analytical phase especially for the specimen collection is very high and this can result in aberration of final laboratory results [2]. To perform a procedure for middle ear fluid collection, the practical guideline has to be used and strictly followed.
you for these kind remarks. We support his observation that specimen collection and preservation are as crucial as the analytic techniques employed for obtaining accurate data. This is particularly important when measuring the biophysical properties of small volumes of secretions as shear forces associated with collection, or degradation associated with inappropriate storage can alter the biophysical and transport properties. We have developed a specific procedure for collection and preservation of these specimens and would be happy to share these robust techniques with readers interested in the collection and analysis of airway secretions. Bruce K. Rubin Department of Pediatrics, Virginia Commonwealth University School of Medicine, Children’s Hospital of Richmond at VCU, 1001 East Marshall Street, PO Box 980646, Richmond, VA 23298 USA Randall Cohen Private Practice, Tucson, Az, USA
References [1] K.M. Dodson, R.S. Cohen, B.K. Rubin, Middle ear fluid characteristics in pediatric otitis media with effusion, Int. J. Pediatr. Otorhinolaryngol. September (2012), http://dx.doi.org/10.1016/j.ijporl.2012.09.005, pii:S0165-5876(12)00511-3 [Epub ahead of print]. [2] V. Wiwanitkit, Types and frequency of preanalytical mistakes in the first Thai ISO 9002:1994 certified clinical laboratory, a 6-month monitoring, BMC Clin. Pathol. 1 (1) (2001) 5. a
Viroj Wiwanitkita,b,c,d,* Wiwanitkit House, Bangkhae, Bangkok, Thailand b Hainan Medical University, China c University of Nis, Serbia d Joseph Ayobabalola University, Nigeria
Kelley M. Dodson* Department of Otolaryngology, Virginia Commonwealth University Health System, Children’s Hospital of Richmond at VCU, USA *Corresponding author. Tel.: +1 804 828 3965; fax: +1 804 828 5779 E-mail address: [email protected] (K.M. Dodson) 8 November 2012
http://dx.doi.org/10.1016/j.ijporl.2012.11.017
*Correspondence at: Wiwanitkit House, Bangkhae, Bangkok, Thailand. Tel.: +66 24132436 E-mail address: [email protected] (V. Wiwanitkit) 6 October 2012
Letter to the Editor
http://dx.doi.org/10.1016/j.ijporl.2012.11.015
Response to the Letter to the Editor
Response to the Letter to the Editor regarding ‘‘Pie-slice tympanoplasty for transcanal removal of small congenital cholesteatoma’’ Dear Editor,
Response to the Letter to the Editor regarding: Middle ear fluid characteristics in pediatric otitis media with effusion To the Editor, We are pleased that Professor Wiwanitkit is enthusiastic about this manuscript and has found this information valuable. Thank 0165-5876/$ – see front matter ß 2012 Elsevier Ireland Ltd. All rights reserved.
We thank Dr. Johannes Borgstein for the comments on our article, ‘‘Pie-slice tympanoplasty for transcanal removal of small congenital cholesteatoma’’. Below are our responses to the three points raised by Dr. Borgstein: Response 1: We do not believe that these were acquired cholesteatomas based on location, size, healthy, mastoid, etc. We