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Rice regenerated plants resistant to oxalysine
Cell Biology
international
Reports,
Vol. 11, No. 8, August
1987
RICE REGENERATED PLANTS RESISTANT TO OXALYSINE He Zhuo-Pei, Xu Shu-Ping, and Loo Shih-Wei* Shanghai Institute of Plant Physiology, Academia Shanghai, China 200032
Sinica
On the basis of the establishment of the tobacco and asparagus cell lines both resistant to 1-4-oxalysine(OL) and accumulating more lysine (Ho & Loo, 1986), the authors extended their experience to rice for getting certain rices with the corresponding traits. Hulled rice (Oryza sativa L. cv. Jingyin 127) caryopses were immersed in 75% ale. for 1 rnr and in calcium hypochlorite solution(38 available chlorine) for 25 min. then rinsed followed by 1% ethyl methanesulfonate for 1 hr. and rinsed the EMS away. After incubating on $MS solidified medium for 3 days in darkness, the embryos were isolated from the EMS-treated caryopses and cultured on R2 medium(Ohira et al. ,1973) to inducing callus. The surviving calli were subcultured on R2 medium containing OL(100 mg/l), none of the original type as the check can survive in this selective medium, for 5 weeks. The OL-resistant and growth active calli were selected out and transfered to MS supplemented with 6-benzylaminopurine 2 mg/l , 3-indoleacetic acid 0.5 mg/l, and OL 100 mg/l medium at 27”C(day) and tO”C(night) under cold white fluorescent light After 1.5 klux 12 hr photoperiod for selection and shoot regeneration. 9 weeks the green shoots were subcultured on MS supplemented with 1-naphthaleneacetic acid 0.1 mg/l, kinetin 0.1 mg/l, and OL 100 mg/l medium for 5 weeks, then transfered to R2 medium with NAA 0.1 mg/l, and kinetin 0.1 mg/l instead of 2,4-D until root formation (about four weeks). Then the rice plantlets were transfered to R2 medium with IAA 1 mg/l and OL 100 mg/l instead of 2,4-D for third selection at Through a week in hydroponic regenerated plant level for 5 weeks. the resistant green plants which roots culture as the hardening period, had been immersed in 1% available chlorine for 1 min. were transplanted Now the rice regenerated plants resisinto paddy pots in a phytotron. tant to oxalysine are flowering. (Fig. 1) another line of rice regenerated plants By using the similar method, resistant to OL from cultivar “Hanfeng” was selected out and cultured too. *To whom correspondence should be sent. research Acknowledgement: This :ras partially supported by the grant National Nature Sciences From the ‘oundation, No. 3861050. ‘-iefcrences I-io Chuaec;Pdui & Loo Shih-Wei (1986) analog-resistant cell Amino ’ mutant. In: D.A. Somers et al.(eds.) VIth International Congress of Plant and Cell Culture Abstracts, Tissue Aug. 3-8, 1986, Minneapolis, pp.77. Xu Loo Shih-Wei, Ho Chue-Pui, Li Wen and Zhu-Yun, Xu Shu-Ping 4-oxalvsine-resistant An 11983) mutant. f ram tobacco. callus. In: Cell and Tissue Culture Techniques Improvement. pp. for Cereal Crop Press, Beijing. Science 255-264. K., Ojima, K. & Fujiwara, Ohira, Studies on the ;ut;;;:zn K. (1973) of rice cell culture. I. growth for rapid defined medium and suspension culture. Plant 1113-1121. iill Physiology, 14, Received:
z/5/87
0309-1651/67/08062~01/$03.00/0
Accepted:
3/J/87 @ 1967 Academic
Press Lt@
international
Reports,
Vol. 11, No. 8, August
1987
RICE REGENERATED PLANTS RESISTANT TO OXALYSINE He Zhuo-Pei, Xu Shu-Ping, and Loo Shih-Wei* Shanghai Institute of Plant Physiology, Academia Shanghai, China 200032
Sinica
On the basis of the establishment of the tobacco and asparagus cell lines both resistant to 1-4-oxalysine(OL) and accumulating more lysine (Ho & Loo, 1986), the authors extended their experience to rice for getting certain rices with the corresponding traits. Hulled rice (Oryza sativa L. cv. Jingyin 127) caryopses were immersed in 75% ale. for 1 rnr and in calcium hypochlorite solution(38 available chlorine) for 25 min. then rinsed followed by 1% ethyl methanesulfonate for 1 hr. and rinsed the EMS away. After incubating on $MS solidified medium for 3 days in darkness, the embryos were isolated from the EMS-treated caryopses and cultured on R2 medium(Ohira et al. ,1973) to inducing callus. The surviving calli were subcultured on R2 medium containing OL(100 mg/l), none of the original type as the check can survive in this selective medium, for 5 weeks. The OL-resistant and growth active calli were selected out and transfered to MS supplemented with 6-benzylaminopurine 2 mg/l , 3-indoleacetic acid 0.5 mg/l, and OL 100 mg/l medium at 27”C(day) and tO”C(night) under cold white fluorescent light After 1.5 klux 12 hr photoperiod for selection and shoot regeneration. 9 weeks the green shoots were subcultured on MS supplemented with 1-naphthaleneacetic acid 0.1 mg/l, kinetin 0.1 mg/l, and OL 100 mg/l medium for 5 weeks, then transfered to R2 medium with NAA 0.1 mg/l, and kinetin 0.1 mg/l instead of 2,4-D until root formation (about four weeks). Then the rice plantlets were transfered to R2 medium with IAA 1 mg/l and OL 100 mg/l instead of 2,4-D for third selection at Through a week in hydroponic regenerated plant level for 5 weeks. the resistant green plants which roots culture as the hardening period, had been immersed in 1% available chlorine for 1 min. were transplanted Now the rice regenerated plants resisinto paddy pots in a phytotron. tant to oxalysine are flowering. (Fig. 1) another line of rice regenerated plants By using the similar method, resistant to OL from cultivar “Hanfeng” was selected out and cultured too. *To whom correspondence should be sent. research Acknowledgement: This :ras partially supported by the grant National Nature Sciences From the ‘oundation, No. 3861050. ‘-iefcrences I-io Chuaec;Pdui & Loo Shih-Wei (1986) analog-resistant cell Amino ’ mutant. In: D.A. Somers et al.(eds.) VIth International Congress of Plant and Cell Culture Abstracts, Tissue Aug. 3-8, 1986, Minneapolis, pp.77. Xu Loo Shih-Wei, Ho Chue-Pui, Li Wen and Zhu-Yun, Xu Shu-Ping 4-oxalvsine-resistant An 11983) mutant. f ram tobacco. callus. In: Cell and Tissue Culture Techniques Improvement. pp. for Cereal Crop Press, Beijing. Science 255-264. K., Ojima, K. & Fujiwara, Ohira, Studies on the ;ut;;;:zn K. (1973) of rice cell culture. I. growth for rapid defined medium and suspension culture. Plant 1113-1121. iill Physiology, 14, Received:
z/5/87
0309-1651/67/08062~01/$03.00/0
Accepted:
3/J/87 @ 1967 Academic
Press Lt@