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Role of tumor associated macrophages in lung cancer patients
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Camptotnecin sodium i~ the form of polyphase liposme was studied in this paper, It is assumed that CS in liposome-entrapped form might have more powerful antitumor activity and less toxicity than free CS. The effects of polyphase liposome of camptothecin sodium (PL-CSA) on the life cycle of Ehrlich ascites carcinoma cells in mice was studied by autoradiographic method. The Tc of the cells was found to be 39.2 hours. The T G , T , TG2 , and T M were 8.6, 24.7, 4.3 an~ i.~ hours, respectively. The growth fraction was 0.84. The experimental results showed that cells in S phase might be killed by PL-CSA 25 mg/kg. The mitotic index was also decreased ~igniflc~Dtly after the treatment. PL-CSA prevented strongly the cells progression from S phase into M phase. By microscopic examination, most cells accumulated in GA phase exhibited remarkable morpholoZ glcal changes, such as swollen, vacuolated nuclei and karyorrhexis, etc. Five hours after the administration of PL-CSA the damaged cells in Go phase were about 9.5%. Under the action of PL-CSA, the syntheses of DNA in vivo was inhibited significantly. Experimental results indicate that PL-CSA is a non-specific agent on the carcinoma cells cycle and that cells in S, G 2 phase are sensitive to this drug.
Expression of H~nan Lymphocite Antigens (HLA) in Human L~,g"- Cancer. . I Facchini I A., Mariani~, E., Mastrorill~ , M., Bragagl~a , R., Milanil, M., Villanacci , V., Ferrone , S., Gozzetti , G. i. Department of Surgery and Pathology, University of Bologna, Italy. 2. Department of Microbiology and Immunology, Valhalla University, New York, U.S.A. The expression of three cytoplasmic membrane antigens (HLA, HLA-Dr, B2-microglobulin ) was investigated in serial sections of twenty-one human lung cancers (non small cell lung cancer) using monoclonal antibodies and the Avidin-Biotin Peroxidase-Complex method. The frequency of positive reactions and the relationship among these antigens were determined. Some of these antigenic expression seem to be related to histological and cytological aspects of tumors and can provide important information about the prognosis and therapy.
Intratumoral Diversity of Phenot)~ie Attrabutes of Squamous Cell Lung Carcinomas: Consequences for Biological Analysis. Olsson, L., Rahbek S~rensen, H. State University Hospital, Copenhagen, Denmark. Cloned cell lines of squamous cell lung carcinomas were established in vitro and the phenotypic diversity analysed in respect to morphology, clonogenicity, antigenicity, and tumorigenicity. A remarkable diversity was found in all parameters, including antigenicity that was studied by the use of monoclonal antibodies with specificity for common cell surface components (histocompatibility angivens) and for antigens with high tumor specificity. In order to study the effect of changes in gene activity and degree of cell maturation on the expression of the phenotypic attributes analysed in this study, the cloned in vitro cell lines sere treated with 5-azacytidine(5-azaC) in order to obtain gene activity and with phorbol ester (TPA) and retinoid acid (RA) in order to induce cell differentiation. 5-AzaC changed the cloning efficiency of the cell lines up to 80 times and could also result in s~blines with both tumorigenic and metastatic activity in nude mice. In contrast, TPA or RA treatment resulted
Role of Tumor Associated Macrophages in Lung Cancer Patients. Takeo, S., Yasumoto, K., Miyazaki, K., Kuda, T., Nagashima, A., Yaita, H., Furukawa, T., Nomoto, K. Second Department of Surgery, Kyushu University Medical SchooL, ~ k a , Japan. Content and antitumor activity of tumor associated macrophages (TAM) were examined in 71 patients with resectable primary lung cancer. TAM was obtained by plastic adherence following trypsinization of surgical specimens. The plastic adherent cells were identified as TAM more than 90% by nonspecific esterase staining and morphological features, Content of TAM was widely distributed from 0.1% to 17.3% of total viable cell counts. Patients having more than 2% of TAM content exhibited 4/29 (13.8%) of recurrence rate. On the other hand, those having less than 2% did 8/26 (30.8%). However, there was no significant difference between them. Cytostatic activity of TAM was assessed by inhibition of DNA synthesis of allogenic lung cancer cell line of QG-56, and was ranged from -56% to 81%. Although 7 of 15 patients showing less than 30% of cytostatic activity have recurrent diseases following surgical treatment, only one of 19 patients showing more than 30% of it does. There was significant difference between them (P<0.005). Thus, the assessment of antitumor activity of TAM (not content of TAM) may give prognostic information of lung cancer patients.
in loss of tumorigenicity in vivo and clonogenicity in vitro. These data thus indicate that the amount of clonogenic/tumorigenic cells in such cell lines is determined by expression of a set of genes that in their expression seems regulated by the methylation pattern of the genome. The consequences for these aspects for attempts to identify genes involved in the tumorigenic behaviour of these cells are outlined. Moreover, the generation of metastatic variants should permit application of molecular biology technology to identify metastasis associated genes.
Camptotnecin sodium i~ the form of polyphase liposme was studied in this paper, It is assumed that CS in liposome-entrapped form might have more powerful antitumor activity and less toxicity than free CS. The effects of polyphase liposome of camptothecin sodium (PL-CSA) on the life cycle of Ehrlich ascites carcinoma cells in mice was studied by autoradiographic method. The Tc of the cells was found to be 39.2 hours. The T G , T , TG2 , and T M were 8.6, 24.7, 4.3 an~ i.~ hours, respectively. The growth fraction was 0.84. The experimental results showed that cells in S phase might be killed by PL-CSA 25 mg/kg. The mitotic index was also decreased ~igniflc~Dtly after the treatment. PL-CSA prevented strongly the cells progression from S phase into M phase. By microscopic examination, most cells accumulated in GA phase exhibited remarkable morpholoZ glcal changes, such as swollen, vacuolated nuclei and karyorrhexis, etc. Five hours after the administration of PL-CSA the damaged cells in Go phase were about 9.5%. Under the action of PL-CSA, the syntheses of DNA in vivo was inhibited significantly. Experimental results indicate that PL-CSA is a non-specific agent on the carcinoma cells cycle and that cells in S, G 2 phase are sensitive to this drug.
Expression of H~nan Lymphocite Antigens (HLA) in Human L~,g"- Cancer. . I Facchini I A., Mariani~, E., Mastrorill~ , M., Bragagl~a , R., Milanil, M., Villanacci , V., Ferrone , S., Gozzetti , G. i. Department of Surgery and Pathology, University of Bologna, Italy. 2. Department of Microbiology and Immunology, Valhalla University, New York, U.S.A. The expression of three cytoplasmic membrane antigens (HLA, HLA-Dr, B2-microglobulin ) was investigated in serial sections of twenty-one human lung cancers (non small cell lung cancer) using monoclonal antibodies and the Avidin-Biotin Peroxidase-Complex method. The frequency of positive reactions and the relationship among these antigens were determined. Some of these antigenic expression seem to be related to histological and cytological aspects of tumors and can provide important information about the prognosis and therapy.
Intratumoral Diversity of Phenot)~ie Attrabutes of Squamous Cell Lung Carcinomas: Consequences for Biological Analysis. Olsson, L., Rahbek S~rensen, H. State University Hospital, Copenhagen, Denmark. Cloned cell lines of squamous cell lung carcinomas were established in vitro and the phenotypic diversity analysed in respect to morphology, clonogenicity, antigenicity, and tumorigenicity. A remarkable diversity was found in all parameters, including antigenicity that was studied by the use of monoclonal antibodies with specificity for common cell surface components (histocompatibility angivens) and for antigens with high tumor specificity. In order to study the effect of changes in gene activity and degree of cell maturation on the expression of the phenotypic attributes analysed in this study, the cloned in vitro cell lines sere treated with 5-azacytidine(5-azaC) in order to obtain gene activity and with phorbol ester (TPA) and retinoid acid (RA) in order to induce cell differentiation. 5-AzaC changed the cloning efficiency of the cell lines up to 80 times and could also result in s~blines with both tumorigenic and metastatic activity in nude mice. In contrast, TPA or RA treatment resulted
Role of Tumor Associated Macrophages in Lung Cancer Patients. Takeo, S., Yasumoto, K., Miyazaki, K., Kuda, T., Nagashima, A., Yaita, H., Furukawa, T., Nomoto, K. Second Department of Surgery, Kyushu University Medical SchooL, ~ k a , Japan. Content and antitumor activity of tumor associated macrophages (TAM) were examined in 71 patients with resectable primary lung cancer. TAM was obtained by plastic adherence following trypsinization of surgical specimens. The plastic adherent cells were identified as TAM more than 90% by nonspecific esterase staining and morphological features, Content of TAM was widely distributed from 0.1% to 17.3% of total viable cell counts. Patients having more than 2% of TAM content exhibited 4/29 (13.8%) of recurrence rate. On the other hand, those having less than 2% did 8/26 (30.8%). However, there was no significant difference between them. Cytostatic activity of TAM was assessed by inhibition of DNA synthesis of allogenic lung cancer cell line of QG-56, and was ranged from -56% to 81%. Although 7 of 15 patients showing less than 30% of cytostatic activity have recurrent diseases following surgical treatment, only one of 19 patients showing more than 30% of it does. There was significant difference between them (P<0.005). Thus, the assessment of antitumor activity of TAM (not content of TAM) may give prognostic information of lung cancer patients.
in loss of tumorigenicity in vivo and clonogenicity in vitro. These data thus indicate that the amount of clonogenic/tumorigenic cells in such cell lines is determined by expression of a set of genes that in their expression seems regulated by the methylation pattern of the genome. The consequences for these aspects for attempts to identify genes involved in the tumorigenic behaviour of these cells are outlined. Moreover, the generation of metastatic variants should permit application of molecular biology technology to identify metastasis associated genes.