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RP009 Dynamics of high virus loads in disseminated adenovirus disease
Wissenschaftliches Programm 55. DGHM-Tagung 29. September-l. Oktober 2003 in Dresden Abstracts - Poster
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Dynamics of high virus loads in disseminated adenovirus disease Helm, A.1; Ebnet, C.1; Harste, G.1; Seidemann, K. 2
iMedizinische Hochschule Hannover; Institut fDr Virologie 2Medizinische Hochschule Hannover; P~diatrische Klinik Disseminated adenovirus infections are a life threatening complication of severe immunsuppression and are associated with high virus loads (104 copies/ml blood). Therefore, we search for adenovirus viremias in immunosuppressed patients by quantitative PCR. Adenovirus viremias were detected in 5 immunosuppressed patients (1 adult, 4 children) and lasted over periods of 17 - 45 days. Very high virus loads were detected in 2 of these patients (10 l° copies/ml) and were associated with a fatal outcome. However, virus loads increased slowly and some patients survived even virus loads of 109 copies/ml for several days. Another patient presented with virus loads (106 copies/ml) for 28 days suggesting the feasibility of antiviral therapy. Virus loads in serum were equal or even higher compared to virus loads in whole (EDTA-)blood indicating that most adenovirus DNA is not cell associated. Probably, virus shedding from infected organs (e.g. bowel, as observed in 2 patients) may cause high virus loads in blood. Species human adenovirus C predominated (4 of 5 patients, type 1 in two patients, type 2 and type 5) but one child suffered from a disseminated infection with human adenovirus A (type 31) which is usually found in gastroenteritis epidemics of children. Conclusion: Very high virus loads in disseminated adenovirus infections are clearly live threatening but several weeks of increasing viremia can be observed in advance. Therefore, therapeutic intervention may be possible.
Review of the influenza season 2 0 0 2 / 2 0 0 3 by the NIC Germany Heckler, R.1; Schweiger, B. 2
1Nieders. Landesgesundheitsamt; Virologie 2RKI; Virologie Influenza is still a severe infectious disease. On average 7000-8000 people die every year due to influenza or its complications. The repeating epidemics are supported by the high variability of the virus. By successive changes of the surface-antigens (drift) new variants are constantly emerging. Therefore long lasting immunity against influenza in general cannot be provided by past infections or vaccination. In order to cope with this problem a new vaccine combination must be prepared every few years. The main task of NIC is the detection of antigenic drift or even more antigenic shift. Diagnostic methods have to be constantly adapted and developed~ The global influenza surveillance is facilitated by close cooperation of WHO (Flunet) with the national reference centres. In Germany the NIC
http://www.dghm.org
433
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g
Dynamics of high virus loads in disseminated adenovirus disease Helm, A.1; Ebnet, C.1; Harste, G.1; Seidemann, K. 2
iMedizinische Hochschule Hannover; Institut fDr Virologie 2Medizinische Hochschule Hannover; P~diatrische Klinik Disseminated adenovirus infections are a life threatening complication of severe immunsuppression and are associated with high virus loads (104 copies/ml blood). Therefore, we search for adenovirus viremias in immunosuppressed patients by quantitative PCR. Adenovirus viremias were detected in 5 immunosuppressed patients (1 adult, 4 children) and lasted over periods of 17 - 45 days. Very high virus loads were detected in 2 of these patients (10 l° copies/ml) and were associated with a fatal outcome. However, virus loads increased slowly and some patients survived even virus loads of 109 copies/ml for several days. Another patient presented with virus loads (106 copies/ml) for 28 days suggesting the feasibility of antiviral therapy. Virus loads in serum were equal or even higher compared to virus loads in whole (EDTA-)blood indicating that most adenovirus DNA is not cell associated. Probably, virus shedding from infected organs (e.g. bowel, as observed in 2 patients) may cause high virus loads in blood. Species human adenovirus C predominated (4 of 5 patients, type 1 in two patients, type 2 and type 5) but one child suffered from a disseminated infection with human adenovirus A (type 31) which is usually found in gastroenteritis epidemics of children. Conclusion: Very high virus loads in disseminated adenovirus infections are clearly live threatening but several weeks of increasing viremia can be observed in advance. Therefore, therapeutic intervention may be possible.
Review of the influenza season 2 0 0 2 / 2 0 0 3 by the NIC Germany Heckler, R.1; Schweiger, B. 2
1Nieders. Landesgesundheitsamt; Virologie 2RKI; Virologie Influenza is still a severe infectious disease. On average 7000-8000 people die every year due to influenza or its complications. The repeating epidemics are supported by the high variability of the virus. By successive changes of the surface-antigens (drift) new variants are constantly emerging. Therefore long lasting immunity against influenza in general cannot be provided by past infections or vaccination. In order to cope with this problem a new vaccine combination must be prepared every few years. The main task of NIC is the detection of antigenic drift or even more antigenic shift. Diagnostic methods have to be constantly adapted and developed~ The global influenza surveillance is facilitated by close cooperation of WHO (Flunet) with the national reference centres. In Germany the NIC
http://www.dghm.org
433