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Screening test for respiratory sensitizing washing powder enzymes
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Information Section
PBTA-1---a novel mutagen Japanese researchers have identified a powerful Ames mutagen in river water sampled downstream from two sewage plants near Kyoto. When tested in Salmonella, its potency in the presence of a mammalian metabolizing system was said to be comparable to that of Glu-I-P, a mutagen formed in cooked foods by pyrolysis of amino acids. The new mutagen was identified as 2-[2-(acetylamino)-4-[bis(2.methoxyethyl) amino]-5-methoxyphenyl]-5amino-7-bromo-4-chloro-2H-benzotriazole, and is suspected of being derived from wastes arising from dye factories in Kyoto city (Nukaya H. et aL, Cl~raical Research m Toxicology, 1997, 10, 1061).
Sensitization to oleth-3-phosphate and oleth-5 In a hair wax Sensitization to polyethylene glycol ethers of oleyl alcohol has been described in a psoriasis patient. He developed a red itchy rash on the hands, scalp and face after almost daily use of a hair dressing product manufactured in Germany. Patch tests with components of the hair wax identified oleth-3phosphate and oleth-5 as the cause of the problem
(Abdullah A. et aL, Contact Dermatitis 1997, 37,
188). Screening test for respiratory sensltlzlng washing powder enzymes Tests carried out in guinea pigs by Procter & Gamble have been used to predict the relative sensitizing potential of detergent enzymes. The animals had different levels of enzyme protein instilled into the windpipe once a week for 10--12 consecutive weeks, and the amount of antibody was measured in the serum and compared with that produced by Alcalase, an enzyme for which effective operating guidelines and an ACGIH threshold limit value (of 60 ng/m 3) exist. The protease Savinasewas equivalent to Alcalase, while subtilisin B was less potent. The amylase, Termamyl, was about 10-fold more potent than Alcalase. Limitation of the levels of Termamyl in commercial products based on the animal data resulted in the number of workers sensitized to Termamyl (as demonstrated by positive skin prick tests) being comparable with the number sensitized to Alcalase (Sarlo K. et aL, Fundamental and Applied Toxicology 1997, 39, 44).
Information Section
PBTA-1---a novel mutagen Japanese researchers have identified a powerful Ames mutagen in river water sampled downstream from two sewage plants near Kyoto. When tested in Salmonella, its potency in the presence of a mammalian metabolizing system was said to be comparable to that of Glu-I-P, a mutagen formed in cooked foods by pyrolysis of amino acids. The new mutagen was identified as 2-[2-(acetylamino)-4-[bis(2.methoxyethyl) amino]-5-methoxyphenyl]-5amino-7-bromo-4-chloro-2H-benzotriazole, and is suspected of being derived from wastes arising from dye factories in Kyoto city (Nukaya H. et aL, Cl~raical Research m Toxicology, 1997, 10, 1061).
Sensitization to oleth-3-phosphate and oleth-5 In a hair wax Sensitization to polyethylene glycol ethers of oleyl alcohol has been described in a psoriasis patient. He developed a red itchy rash on the hands, scalp and face after almost daily use of a hair dressing product manufactured in Germany. Patch tests with components of the hair wax identified oleth-3phosphate and oleth-5 as the cause of the problem
(Abdullah A. et aL, Contact Dermatitis 1997, 37,
188). Screening test for respiratory sensltlzlng washing powder enzymes Tests carried out in guinea pigs by Procter & Gamble have been used to predict the relative sensitizing potential of detergent enzymes. The animals had different levels of enzyme protein instilled into the windpipe once a week for 10--12 consecutive weeks, and the amount of antibody was measured in the serum and compared with that produced by Alcalase, an enzyme for which effective operating guidelines and an ACGIH threshold limit value (of 60 ng/m 3) exist. The protease Savinasewas equivalent to Alcalase, while subtilisin B was less potent. The amylase, Termamyl, was about 10-fold more potent than Alcalase. Limitation of the levels of Termamyl in commercial products based on the animal data resulted in the number of workers sensitized to Termamyl (as demonstrated by positive skin prick tests) being comparable with the number sensitized to Alcalase (Sarlo K. et aL, Fundamental and Applied Toxicology 1997, 39, 44).